| 质粒名称: | Mfn2-Myc |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 23213 |
| 存储实验室: | David Chan |
|
载体骨架基本信息 |
|
| 载体名称: | pcDNA3.1(-)/Myc-His |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 5000 bp |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | -- |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | Mfn2 |
| 物种来源: | M. musculus (mouse) |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | Myc（插入件上的C端子） |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | BamHI（销毁后未知） |
| 5' 测序引物 | CMV-F |
| 3' 测序引物 | BGH-rev |
|
文献引用方法 |
|
| 材料和方法部分: | Mfn2-Myc was a gift from David Chan (Addgene plasmid # 23213 ; http://n2t.net/addgene:23213 ; RRID:Addgene_23213) |
| 参考文献部分: | Mitofusins Mfn1 and Mfn2 coordinately regulate mitochondrial fusion and are essential for embryonic development. Chen H, Detmer SA, Ewald AJ, Griffin EE, Fraser SE, Chan DC. J Cell Biol. 2003 Jan 20. 160(2):189-200. 10.1083/jcb.200211046 PubMed 12527753 |
买家导航
Mfn2Myc载体基本信息
Mfn2Myc载体图谱质粒图谱和多克隆位点信息
Mfn2Myc载体简介
Mfn2Myc载体序列
LOCUS Mfn2-Myc 8419 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS Mfn2-Myc
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8419)
AUTHORS Chen H, Detmer SA, Ewald AJ, Griffin EE, Fraser SE, Chan DC
TITLE Mitofusins Mfn1 and Mfn2 coordinately regulate mitochondrial fusion
and are essential for embryonic development.
JOURNAL J Cell Biol. 2003 Jan 20. 160(2):189-200.
PUBMED 12527753
REFERENCE 2 (bases 1 to 8419)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..8419
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind complement(44..63)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
enhancer 235..614
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 615..818
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 769..789
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind 863..882
/label=T7
/note="T7 promoter, forward primer"
promoter 863..881
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
CDS 3288..3317
/codon_start=1
/product="Myc (human c-Myc proto-oncogene) epitope tag"
/label=Myc
/translation="EQKLISEEDL"
CDS 3324..3353
/codon_start=1
/product="Myc (human c-Myc proto-oncogene) epitope tag"
/label=Myc
/translation="EQKLISEEDL"
CDS 3360..3389
/codon_start=1
/product="Myc (human c-Myc proto-oncogene) epitope tag"
/label=Myc
/translation="EQKLISEEDL"
CDS 3408..3437
/codon_start=1
/product="Myc (human c-Myc proto-oncogene) epitope tag"
/label=Myc
/translation="EQKLISEEDL"
CDS 3444..3473
/codon_start=1
/product="Myc (human c-Myc proto-oncogene) epitope tag"
/label=Myc
/translation="EQKLISEEDL"
CDS 3480..3509
/codon_start=1
/product="Myc (human c-Myc proto-oncogene) epitope tag"
/label=Myc
/translation="EQKLISEEDL"
CDS 3528..3557
/codon_start=1
/product="Myc (human c-Myc proto-oncogene) epitope tag"
/label=Myc
/translation="EQKLISEEDL"
CDS 3564..3593
/codon_start=1
/product="Myc (human c-Myc proto-oncogene) epitope tag"
/label=Myc
/translation="EQKLISEEDL"
CDS 3600..3629
/codon_start=1
/product="Myc (human c-Myc proto-oncogene) epitope tag"
/label=Myc
/translation="EQKLISEEDL"
CDS 3648..3677
/codon_start=1
/product="Myc (human c-Myc proto-oncogene) epitope tag"
/label=Myc
/translation="EQKLISEEDL"
CDS 3684..3713
/codon_start=1
/product="Myc (human c-Myc proto-oncogene) epitope tag"
/label=Myc
/translation="EQKLISEEDL"
CDS 3720..3749
/codon_start=1
/product="Myc (human c-Myc proto-oncogene) epitope tag"
/label=Myc
/translation="EQKLISEEDL"
CDS 3768..3797
/codon_start=1
/product="Myc (human c-Myc proto-oncogene) epitope tag"
/label=Myc
/translation="EQKLISEEDL"
CDS 3804..3833
/codon_start=1
/product="Myc (human c-Myc proto-oncogene) epitope tag"
/label=Myc
/translation="EQKLISEEDL"
CDS 3840..3869
/codon_start=1
/product="Myc (human c-Myc proto-oncogene) epitope tag"
/label=Myc
/translation="EQKLISEEDL"
CDS 3903..3932
/codon_start=1
/product="Myc (human c-Myc proto-oncogene) epitope tag"
/label=Myc
/translation="EQKLISEEDL"
CDS 3948..3965
/codon_start=1
/product="6xHis affinity tag"
/label=6xHis
/translation="HHHHHH"
primer_bind complement(4009..4026)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 4015..4239
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 4285..4713
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(4372..4391)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 4582..4603
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind complement(4722..4742)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 4727..5057
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 4908..5043
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 4970..4989
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
CDS 5124..5918
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(5178..5197)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 5788..5807
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
polyA_signal 6092..6213
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(6129..6148)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 6183..6202
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind complement(6262..6278)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(6262..6278)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(6275..6297)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 6286..6302
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(6310..6340)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 6355..6376
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(6493..6510)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(6664..7252)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(6744..6763)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(7423..8283)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 8046..8065
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(8284..8388)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt
481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
901 gtttaaacgg gccctctaga ctcgagtcct tttcatcttt ctgactccag ccatgtccac
961 gatgcccaac ctgtgaaggt ctcttaccag ctagaaacga gatgtccctg ctcttttctc
1021 gatgcaactc catcgtcacc gtcaagaagg ataagcgaca catggctgaa gtgaatgctt
1081 cccctctcaa gcactttgtc actgccaaga aaaagatcaa tggaatcttt gagcagctgg
1141 gggcctacat ccaagagagc gccagcttcc ttgaagacac ccacaggaac acagaactgg
1201 acccggttac cacggaagag caggtcctgg acgtcaaagg gtacctgtcc aaggtcaggg
1261 gtatcagcga agtgctggcc aggcggcaca tgaaggtggc tttttttggc cggacgagca
1321 atgggaagag caccgtgatc aatgccatgc tctgggacaa agttctgcca tctgggattg
1381 gtcataccac caattgcttc ctgcgggttg ggggcacaga tggccatgag gccttcctcc
1441 tcacagaggg ctcagaagag aagaagagtg tcaagactgt gaaccaactg gcccatgccc
1501 tccatcagga cgagcagttg catgcaggca gcatggtgag tgtgatgtgg cccaactcca
1561 agtgtccgct cctgaaggat gacctcgtgc tgatggacag ccctgggatc gatgttacca
1621 cggagctgga cagctggatt gataagtttt gcctggatgc tgatgtgttt gtgctggtgg
1681 ccaactcaga gtccacgctg atgcagacgg agaagcagtt cttccacaaa gtgagtgaac
1741 gtctctcccg gcccaacatc ttcatcctga acaaccgctg ggatgcgtct gcctcggagc
1801 ctgagtacat ggaggaggtg cggcggcagc acatggagcg ctgcaccagc tttctggtgg
1861 atgagctggg cgtggtggat cgagctcagg ctggggaccg gatcttcttc gtgtctgcca
1921 aggaggttct cagcgccagg gtccagaaag cccagggcat gccagaagga ggcggcgctc
1981 tcgcagaagg ttttcaagtg aggatgtttg agtttcagaa tttcgagagg cagtttgagg
2041 agtgcatttc ccagtctgca gtaaagacca aatttgagca gcacacagtc cgggccaagc
2101 agattgcaga ggccgttcgt ctcatcatgg attccctgca catcgcagct caggagcagc
2161 gggtttattg cctagaaatg cgggaagagc ggcaagaccg gctgaggttt attgacaagc
2221 agctggagct cctggctcaa gactacaagc tgcgaattaa gcagattacg gaggaagtgg
2281 aaaggcaggt gtccacagcc atggctgaag agatcaggcg cctctctgtg ctagttgacg
2341 agtaccagat ggacttccac ccatccccag ttgtcctcaa ggtttataag aacgagctgc
2401 accgccatat agaggaaggt ctgggccgga acctgtctga ccgctgctcc actgccattg
2461 ccagttcact gcagactatg cagcaggaca tgatagacgg cttgaagccc cttcttcctg
2521 tatctatgcg gaatcagata gacatgctgg tccctcgaca gtgtttctcc ctcagctatg
2581 acctgaattg tgacaagctg tgtgctgact ttcaggagga catcgagttc cacttctccc
2641 ttggatggac tatgctagtg aacaggttcc tgggccccaa gaatagccgc cgggccttgc
2701 taggctacag tgatcaggtt cagcgtcctc tccctctgac acctgccaac cccagcatgc
2761 cccccttgcc acagagctcc ctcacccagg aggagctcat ggtctccatg gttactggcc
2821 tggcctctct gacgtctagg acctccatgg gcattcttgt ggtcggagga gtggtgtgga
2881 aggcagtggg ctggagactc atcgccctct cctttggact gtatggcctc ctgtacgtct
2941 atgagcgact gacctggacc accaaagcca aagagagggc cttcaagcgc cagtttgtgg
3001 aatacgccag tgagaagcta cagctcatca tcagttacac cggctctaac tgcagccacc
3061 aagtccagca ggaattgtct gggacatttg ctcatctgtg ccagcaagtt gacatcaccc
3121 gagataatct ggagcaggaa attgctgcca tgaacaagaa agtcgaggct ctggattcac
3181 ttcagagcag agccaaactg ctcaggaata aagctggctg gttggacagc gaactcaaca
3241 tgttcacaca ccagtacctg cagcccagca gaggatcctc tagaggtgaa caaaagttga
3301 tttctgaaga agatttgaac ggtgaacaaa agctaatctc cgaggaagac ttgaacggtg
3361 aacaaaaatt aatctcagaa gaagacttga acggatcctc tagaggtgaa caaaagttga
3421 tttctgaaga agatttgaac ggtgaacaaa agctaatctc cgaggaagac ttgaacggtg
3481 aacaaaaatt aatctcagaa gaagacttga acggatcctc tagaggtgaa caaaagttga
3541 tttctgaaga agatttgaac ggtgaacaaa agctaatctc cgaggaagac ttgaacggtg
3601 aacaaaaatt aatctcagaa gaagacttga acggatcctc tagaggtgaa caaaagttga
3661 tttctgaaga agatttgaac ggtgaacaaa agctaatctc cgaggaagac ttgaacggtg
3721 aacaaaaatt aatctcagaa gaagacttga acggatcctc tagaggtgaa caaaagttga
3781 tttctgaaga agatttgaac ggtgaacaaa agctaatctc cgaggaagac ttgaacggtg
3841 aacaaaaatt aatctcagaa gaagacttga acggatccga gctcggtacc aagcttgggc
3901 ccgaacaaaa actcatctca gaagaggatc tgaatagcgc cgtcgaccat catcatcatc
3961 atcattgagt ttaaacggtc tccagcttaa gtttaaaccg ctgatcagcc tcgactgtgc
4021 cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag
4081 gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta
4141 ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag
4201 acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg gaaagaacca
4261 gctggggctc tagggggtat ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg
4321 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg
4381 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
4441 gcatcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
4501 agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt
4561 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta
4621 tctcggtcta ttcttttgat ttataaggga ttttggggat ttcggcctat tggttaaaaa
4681 atgagctgat ttaacaaaaa tttaacgcga attaattctg tggaatgtgt gtcagttagg
4741 gtgtggaaag tccccaggct ccccaggcag gcagaagtat gcaaagcatg catctcaatt
4801 agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
4861 tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa
4921 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
4981 aggccgaggc cgcctctgcc tctgagctat tccagaagta gtgaggaggc ttttttggag
5041 gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc cattttcgga tctgatcaag
5101 agacaggatg aggatcgttt cgcatgattg aacaagatgg attgcacgca ggttctccgg
5161 ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg
5221 atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc
5281 tgtccggtgc cctgaatgaa ctgcaggacg aggcagcgcg gctatcgtgg ctggccacga
5341 cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc
5401 tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag
5461 tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat
5521 tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg
5581 tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca
5641 ggctcaaggc gcgcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct
5701 tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg
5761 gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg
5821 gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc
5881 gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc gggactctgg ggttcgaaat
5941 gaccgaccaa gcgacgccca acctgccatc acgagatttc gattccaccg ccgccttcta
6001 tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg
6061 ggatctcatg ctggagttct tcgcccaccc caacttgttt attgcagctt ataatggtta
6121 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
6181 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tgtataccgt cgacctctag
6241 ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
6301 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
6361 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
6421 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
6481 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
6541 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
6601 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
6661 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
6721 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
6781 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
6841 aagcgtggcg ctttctcaat gctcacgctg taggtatctc agttcggtgt aggtcgttcg
6901 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
6961 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
7021 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
7081 gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt
7141 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
7201 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
7261 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
7321 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
7381 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
7441 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
7501 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
7561 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
7621 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
7681 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
7741 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
7801 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
7861 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
7921 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
7981 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
8041 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
8101 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
8161 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
8221 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
8281 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
8341 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
8401 aaaagtgcca cctgacgtc
//
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Mfn2Myc载体质粒应用举例
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