| 质粒名称: | NGFR |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 27489 |
| 存储实验室: | Warren Pear |
|
载体骨架基本信息 |
|
| 载体名称: | MSCV2.2 (MIG) |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | -- |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳动物表达，逆转录病毒 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | -- |
| 物种来源: | H. sapiens (human) |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: |
ECMV（插接件上的C端子）
IRES（插接件上的C端子）
NGFR（插入件上的C端子）
|
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | pLXSN 5' |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | NGFR was a gift from Warren Pear (Addgene plasmid # 27489 ; http://n2t.net/addgene:27489 ; RRID:Addgene_27489) |
| 参考文献部分: | Notch1 regulates maturation of CD4+ and CD8+ thymocytes by modulating TCR signal strength. Izon DJ, Punt JA, Xu L, Karnell FG, Allman D, Myung PS, Boerth NJ, Pui JC, Koretzky GA, Pear WS. Immunity. 2001 Mar . 14(3):253-64. 10.1016/S1074-7613(01)00107-8 PubMed 11290335 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS NGFR 6521 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS NGFR
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6521)
AUTHORS Izon DJ, Punt JA, Xu L, Karnell FG, Allman D, Myung PS, Boerth NJ,
Pui JC, Koretzky GA, Pear WS
TITLE Notch1 regulates maturation of CD4+ and CD8+ thymocytes by
modulating TCR signal strength.
JOURNAL Immunity. 2001 Mar . 14(3):253-64.
PUBMED 11290335
REFERENCE 2 (bases 1 to 6521)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..6521
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind complement(2..21)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(681..1541)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 1304..1323
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(1542..1646)
/gene="bla"
/label=AmpR promoter
primer_bind 1714..1732
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
primer_bind complement(1770..1792)
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind 1892..1911
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 2105..2127
/label=M13/pUC Forward
/note="In lacZ gene"
primer_bind 2255..2274
/label=pBRrevBam
/note="pBR322 vectors, tet region, downstream of BamHI,
reverse primer"
LTR 2474..2990
/label=5' LTR
/note="5' long terminal repeat from murine embryonic stem
cell virus"
misc_feature 3054..3395
/label=MESV Psi
/note="packaging signal of murine embryonic stem cell
virus"
CDS 3462..3878
/label=gag (truncated)
/note="truncated Moloney murine leukemia virus (MMLV) gag
gene lacking the start codon"
primer_bind 3808..3830
/label=pLXSN 5'
/note="Murine stem cell virus, forward primer. Also called
MSCV"
primer_bind 3846..3862
/label=pBABE 5'
/note="Psi packaging signal, forward primer for pBABE
vectors"
misc_feature 3923..4475
/label=IRES
/note="internal ribosome entry site (IRES) of the
encephalomyocarditis virus (EMCV)"
primer_bind complement(4067..4084)
/label=IRES reverse
/note="IRES internal ribosome entry site, reverse primer.
Also called pCDH-rev"
primer_bind 4294..4313
/label=IRES-F
/note="IRES internal ribosome entry site, forward primer"
LTR 5389..5903
/label=3' LTR
/note="3' long terminal repeat from murine embryonic stem
cell virus"
protein_bind 6065..6081
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(6089..6119)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 6134..6155
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(6272..6289)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(6443..510)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
61 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
121 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
181 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
241 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
301 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
361 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
421 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
481 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
541 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
601 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
661 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
721 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
781 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
841 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
901 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
961 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
1021 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
1081 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
1141 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
1201 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
1261 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
1321 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
1381 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
1441 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
1501 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
1561 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
1621 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa
1681 gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt
1741 ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc
1801 acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt
1861 gttggcgggt gtcggggctg gcttaactat gcggcatcag agcagattgt actgagagtg
1921 caccatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggcgc
1981 cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta
2041 ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg
2101 ttttcccagt cacgacgttg taaaacgacg gcgcaaggaa tggtgcatgc aaggagatgg
2161 cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca
2221 tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag
2281 caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggcgattag
2341 tccaatttgt taaagacagg atatcagtgg tccaggctct agttttgact caacaatatc
2401 accagctgaa gcctatagag tacgagccat agataaaata aaagatttta tttagtctcc
2461 agaaaaaggg gggaatgaaa gaccccacct gtaggtttgg caagctagct taagtaacgc
2521 cattttgcaa ggcatggaaa atacataact gagaatagag aagttcagat caaggttagg
2581 aacagagaga cagcagaata tgggccaaac aggatatctg tggtaagcag ttcctgcccc
2641 ggctcagggc caagaacaga tggtccccag atgcggtccc gccctcagca gtttctagag
2701 aaccatcaga tgtttccagg gtgccccaag gacctgaaat gaccctgtgc cttatttgaa
2761 ctaaccaatc agttcgcttc tcgcttctgt tcgcgcgctt ctgctccccg agctcaataa
2821 aagagcccac aacccctcac tcggcgcgcc agtcctccga tagactgcgt cgcccgggta
2881 cccgtattcc caataaagcc tcttgctgtt tgcatccgaa tcgtggactc gctgatcctt
2941 gggagggtct cctcagattg attgactgcc cacctcgggg gtctttcatt tggaggttcc
3001 accgagattt ggagacccct gcccagggac caccgacccc cccgccggga ggtaagctgg
3061 ccagcggtcg tttcgtgtct gtctctgtct ttgtgcgtgt ttgtgccggc atctaatgtt
3121 tgcgcctgcg tctgtactag ttagctaact agctctgtat ctggcggacc cgtggtggaa
3181 ctgacgagtt ctgaacaccc ggccgcaacc ctgggagacg tcccagggac tttgggggcc
3241 gtttttgtgg cccgacctga ggaagggagt cgatgtggaa tccgaccccg tcaggatatg
3301 tggttctggt aggagacgag aacctaaaac agttcccgcc tccgtctgaa tttttgcttt
3361 cggtttggaa ccgaagccgc gcgtcttgtc tgctgcagcg ctgcagcatc gttctgtgtt
3421 gtctctgtct gactgtgttt ctgtatttgt ctgaaaatta gggccagact gttaccactc
3481 ccttaagttt gaccttaggt cactggaaag atgtcgagcg gatcgctcac aaccagtcgg
3541 tagatgtcaa gaagagacgt tgggttacct tctgctctgc agaatggcca acctttaacg
3601 tcggatggcc gcgagacggc acctttaacc gagacctcat cacccaggtt aagatcaagg
3661 tcttttcacc tggcccgcat ggacacccag accaggtccc ctacatcgtg acctgggaag
3721 ccttggcttt tgacccccct ccctgggtca agccctttgt acaccctaag cctccgcctc
3781 ctcttcctcc atccgccccg tctctccccc ttgaacctcc tcgttcgacc ccgcctcgat
3841 cctcccttta tccagccctc actccttctc taggcgccgg aattagatct ctcgaggtta
3901 acgaattccg cccccccccc taacgttact ggccgaagcc gcttggaata aggccggtgt
3961 gcgtttgtct atatgttatt ttccaccata ttgccgtctt ttggcaatgt gagggcccgg
4021 aaacctggcc ctgtcttctt gacgagcatt cctaggggtc tttcccctct cgccaaagga
4081 atgcaaggtc tgttgaatgt cgtgaaggaa gcagttcctc tggaagcttc ttgaagacaa
4141 acaacgtctg tagcgaccct ttgcaggcag cggaaccccc cacctggcga caggtgcctc
4201 tgcggccaaa agccacgtgt ataagataca cctgcaaagg cggcacaacc ccagtgccac
4261 gttgtgagtt ggatagttgt ggaaagagtc aaatggctct cctcaagcgt attcaacaag
4321 gggctgaagg atgcccagaa ggtaccccat tgtatgggat ctgatctggg gcctcggtgc
4381 acatgcttta catgtgttta gtcgaggtta aaaaaacgtc taggcccccc gaaccacggg
4441 gacgtggttt tcctttgaaa aacacgatga taatatggcc acaaccatgg acgggccgcg
4501 cctgctgctg ttgctgcttc tgggggtgtc ccttggaggt gccaaggagg catgccccac
4561 aggcctgtac acacacagcg gtgagtgctg caaagcctgc aacctgggcg agggtgtggc
4621 ccagccttgt ggagccaacc agaccgtgtg tgagccctgc ctggacagcg tgacgttctc
4681 cgacgtggtg agcgcgaccg agccgtgcaa gccgtgcacc gagtgcgtgg ggctccagag
4741 catgtcggcg ccgtgcgtgg aggccgacga cgccgtgtgc cgctgcgcct acggctacta
4801 ccaggatgag acgactgggc gctgcgaggc gtgccgcgtg tgcgaggcgg gctcgggcct
4861 cgtgttctcc tgccaggaca agcagaacac cgtgtgcgag gagtgccccg acggcacgta
4921 ttccgacgag gccaaccacg tggacccgtg cctgccctgc accgtgtgcg aggacaccga
4981 gcgccagctc cgcgagtgca cacgctgggc cgacgccgag tgcgaggaga tccctggccg
5041 ttggattaca cggtccacac ccccagaggg ctcggacagc acagccccca gcacccagga
5101 gcctgaggca cctccagaac aagacctcat agccagcacg gtggcaggtg tggtgaccac
5161 agtgatgggc agctcccagc ccgtggtgac ccgaggcacc accgacaacc tcatccctgt
5221 ctattgctcc atcctggctg ctgtggttgt gggccttgtg gcctacatag ccttcaagag
5281 gtggaacagt catcgatatc ctcgaggtca ccgcggtcta gagtcgacct gcagccaagc
5341 ttatcgataa aataaaagat tttatttagt ctccagaaaa aggggggaat gaaagacccc
5401 acctgtaggt ttggcaagct agcttaagta acgccatttt gcaaggcatg gaaaatacat
5461 aactgagaat agagaagttc agatcaaggt taggaacaga gagacagcag aatatgggcc
5521 aaacaggata tctgtggtaa gcagttcctg ccccggctca gggccaagaa cagatggtcc
5581 ccagatgcgg tcccgccctc agcagtttct agagaaccat cagatgtttc cagggtgccc
5641 caaggacctg aaatgaccct gtgccttatt tgaactaacc aatcagttcg cttctcgctt
5701 ctgttcgcgc gcttctgctc cccgagctca ataaaagagc ccacaacccc tcactcggcg
5761 cgccagtcct ccgatagact gcgtcgcccg ggtacccgtg tatccaataa accctcttgc
5821 agttgcatcc gacttgtggt ctcgctgttc cttgggaggg tctcctctga gtgattgact
5881 acccgtcagc gggggtcttt catgggtaac agtttcttga agttggagaa caacattctg
5941 agggtaggag tcgaatatta agtaatcctg actcaattag ccactgtttt gaatccacat
6001 actccaatac tcctgaaata gttcattatg gacagcgcag aaagagctgg ggagaattgt
6061 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
6121 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
6181 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
6241 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
6301 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
6361 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
6421 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
6481 atcgacgctc aagtcagagg tggcgaaacc cgacaggact a
//
