| 质粒名称: | lenti sgRNA(MS2)_zeo backbone |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 61427 |
| 存储实验室: | Feng Zhang |
|
载体骨架基本信息 |
|
| 载体名称: | plenti |
| 载体抗性: | 氨苄霉素 |
| 载体类型: | 哺乳细胞表达,慢病毒 |
| 筛选标记: | 博莱霉素 |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 启动子 | U6 and EF1A |
| 克隆方法: | 限制性内切酶 |
| 5' 测序引物 | GAG GGC CTA TTT CCC ATG ATT CCT TCA TAT |
| 3' 测序引物 | cctagaaggtccattagctgcaaagattcc |
|
文献引用方法 |
|
| 材料和方法部分: | lenti sgRNA(MS2)_zeo backbone was a gift from Feng Zhang (Addgene plasmid # 61427) |
| 参考文献部分: | Genome-scale transcriptional activation by an engineered CRISPR-Cas9 complex. Konermann S, Brigham MD, Trevino AE, Joung J, Abudayyeh OO, Barcena C, Hsu PD, Habib N, Gootenberg JS, Nishimasu H, Nureki O, Zhang F. Nature. 2014 Dec 10. doi: 10.1038/nature14136. 10.1038/nature14136 PubMed 25494202 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
_zeo backbone质粒图谱.png)
载体简介
载体序列
LOCUS lenti sgRNA(MS2)_zeo backbone Sequencing Result 9997 bp DNA SYN
DEFINITION lenti sgRNA(MS2)_zeo backbone Sequencing Result
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
FEATURES Location/Qualifiers
source 1..9997
/organism="lenti sgRNA(MS2)_zeo backbone Sequencing Result"
/mol_type="other DNA"
misc_feature complement(13..33)
/label="pBABE_3_primer"
misc_feature complement(19..234)
/label="SV40_enhancer"
promoter 31..299
/label="SV40_promoter"
rep_origin 198..275
/label="SV40_origin"
misc_feature 260..279
/label="SV40pro_F_primer"
promoter 393..460
/label="EM7_promoter"
gene 461..832
/label="bleo"
/gene="bleo"
misc_feature 461..835
/label="sh_ble"
terminator 968..1087
/label="SV40_PA_terminator"
misc_feature 1056..1075
/label="EBV_rev_primer"
promoter complement(1131..1149)
/label="M13_reverse_primer"
misc_feature complement(1148..1170)
/label="M13_pUC_rev_primer"
promoter complement(1184..1213)
/label="lac_promoter"
gene complement(2296..3156)
/label="Ampicillin"
/gene="Ampicillin"
CDS complement(2296..3156)
/label="ORF frame 2"
promoter complement(3198..3226)
/label="AmpR_promoter"
promoter 3528..4104
/label="CMV_immearly_promoter"
misc_feature 3607..3894
/label="CAG_enhancer"
misc_feature 4061..4081
/label="CMV_fwd_primer"
misc_feature 4124..4304
/label="HIV-1_5_LTR"
misc_feature 4124..4304
/label="truncHIV-1_3_LTR"
misc_feature 4415..4459
/label="HIV-1_psi_pack"
CDS 4847..5734
/label="ORF frame 2"
misc_feature 4969..5202
/label="RRE"
misc_feature 5733..5748
/label="cPPT"
promoter 5905..6137
/label="hU6_promoter"
misc_feature 6067..6086
/label="LKO1_5_primer"
promoter 6401..7572
/label="EF1a_promoter"
CDS complement(6692..7312)
/label="ORF frame 1"
misc_feature 7520..7540
/label="EF1a_fwd_primer"
misc_feature 7579..7953
/label="sh_ble"
gene 7579..7950
/label="bleo"
/gene="bleo"
misc_feature 7980..8567
/label="WPRE"
CDS 8068..9117
/label="ORF frame 1"
CDS 8081..8593
/label="ORF frame 2"
misc_feature complement(8570..8586)
/label="pBluescriptKS_primer"
misc_feature 8754..8769
/label="cPPT"
misc_feature 8754..8775
/label="U3PPT"
misc_feature 9091..9271
/label="HIV-1_5_LTR"
misc_feature 9091..9271
/label="truncHIV-1_3_LTR"
misc_feature complement(9297..9314)
/label="BGH_rev_primer"
terminator 9300..9527
/label="bGH_PA_terminator"
rep_origin 9590..9896
/label="f1_origin"
ORIGIN
1 AATTAATTCT GTGGAATGTG TGTCAGTTAG GGTGTGGAAA GTCCCCAGGC TCCCCAGCAG
61 GCAGAAGTAT GCAAAGCATG CATCTCAATT AGTCAGCAAC CAGGTGTGGA AAGTCCCCAG
121 GCTCCCCAGC AGGCAGAAGT ATGCAAAGCA TGCATCTCAA TTAGTCAGCA ACCATAGTCC
181 CGCCCCTAAC TCCGCCCATC CCGCCCCTAA CTCCGCCCAG TTCCGCCCAT TCTCCGCCCC
241 ATGGCTGACT AATTTTTTTT ATTTATGCAG AGGCCGAGGC CGCCTCTGCC TCTGAGCTAT
301 TCCAGAAGTA GTGAGGAGGC TTTTTTGGAG GCCTAGGCTT TTGCAAAAAG CTCCCGGGAG
361 CTTGTATATC CATTTTCGGA TCTGATCAGC ACGTGTTGAC AATTAATCAT CGGCATAGTA
421 TATCGGCATA GTATAATACG ACAAGGTGAG GAACTAAACC ATGGCCAAGT TGACCAGTGC
481 CGTTCCGGTG CTCACCGCGC GCGACGTCGC CGGAGCGGTC GAGTTCTGGA CCGACCGGCT
541 CGGGTTCTCC CGGGACTTCG TGGAGGACGA CTTCGCCGGT GTGGTCCGGG ACGACGTGAC
601 CCTGTTCATC AGCGCGGTCC AGGACCAGGT GGTGCCGGAC AACACCCTGG CCTGGGTGTG
661 GGTGCGCGGC CTGGACGAGC TGTACGCCGA GTGGTCGGAG GTCGTGTCCA CGAACTTCCG
721 GGACGCCTCC GGGCCGGCCA TGACCGAGAT CGGCGAGCAG CCGTGGGGGC GGGAGTTCGC
781 CCTGCGCGAC CCGGCCGGCA ACTGCGTGCA CTTCGTGGCC GAGGAGCAGG ACTGACACGT
841 GCTACGAGAT TTCGATTCCA CCGCCGCCTT CTATGAAAGG TTGGGCTTCG GAATCGTTTT
901 CCGGGACGCC GGCTGGATGA TCCTCCAGCG CGGGGATCTC ATGCTGGAGT TCTTCGCCCA
961 CCCCAACTTG TTTATTGCAG CTTATAATGG TTACAAATAA AGCAATAGCA TCACAAATTT
1021 CACAAATAAA GCATTTTTTT CACTGCATTC TAGTTGTGGT TTGTCCAAAC TCATCAATGT
1081 ATCTTATCAT GTCTGTATAC CGTCGACCTC TAGCTAGAGC TTGGCGTAAT CATGGTCATA
1141 GCTGTTTCCT GTGTGAAATT GTTATCCGCT CACAATTCCA CACAACATAC GAGCCGGAAG
1201 CATAAAGTGT AAAGCCTGGG GTGCCTAATG AGTGAGCTAA CTCACATTAA TTGCGTTGCG
1261 CTCACTGCCC GCTTTCCAGT CGGGAAACCT GTCGTGCCAG CTGCATTAAT GAATCGGCCA
1321 ACGCGCGGGG AGAGGCGGTT TGCGTATTGG GCGCTCTTCC GCTTCCTCGC TCACTGACTC
1381 GCTGCGCTCG GTCGTTCGGC TGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG
1441 GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA
1501 GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA
1561 CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG
1621 ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT
1681 TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG
1741 CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC
1801 CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT
1861 AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA
1921 TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC
1981 AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC
2041 TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT
2101 TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC
2161 TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT
2221 CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA
2281 AACTTGGTCT GACAGTTACC AATGCTTAAT CAGTGAGGCA CCTATCTCAG CGATCTGTCT
2341 ATTTCGTTCA TCCATAGTTG CCTGACTCCC CGTCGTGTAG ATAACTACGA TACGGGAGGG
2401 CTTACCATCT GGCCCCAGTG CTGCAATGAT ACCGCGAGAC CCACGCTCAC CGGCTCCAGA
2461 TTTATCAGCA ATAAACCAGC CAGCCGGAAG GGCCGAGCGC AGAAGTGGTC CTGCAACTTT
2521 ATCCGCCTCC ATCCAGTCTA TTAATTGTTG CCGGGAAGCT AGAGTAAGTA GTTCGCCAGT
2581 TAATAGTTTG CGCAACGTTG TTGCCATTGC TACAGGCATC GTGGTGTCAC GCTCGTCGTT
2641 TGGTATGGCT TCATTCAGCT CCGGTTCCCA ACGATCAAGG CGAGTTACAT GATCCCCCAT
2701 GTTGTGCAAA AAAGCGGTTA GCTCCTTCGG TCCTCCGATC GTTGTCAGAA GTAAGTTGGC
2761 CGCAGTGTTA TCACTCATGG TTATGGCAGC ACTGCATAAT TCTCTTACTG TCATGCCATC
2821 CGTAAGATGC TTTTCTGTGA CTGGTGAGTA CTCAACCAAG TCATTCTGAG AATAGTGTAT
2881 GCGGCGACCG AGTTGCTCTT GCCCGGCGTC AATACGGGAT AATACCGCGC CACATAGCAG
2941 AACTTTAAAA GTGCTCATCA TTGGAAAACG TTCTTCGGGG CGAAAACTCT CAAGGATCTT
3001 ACCGCTGTTG AGATCCAGTT CGATGTAACC CACTCGTGCA CCCAACTGAT CTTCAGCATC
3061 TTTTACTTTC ACCAGCGTTT CTGGGTGAGC AAAAACAGGA AGGCAAAATG CCGCAAAAAA
3121 GGGAATAAGG GCGACACGGA AATGTTGAAT ACTCATACTC TTCCTTTTTC AATATTATTG
3181 AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA
3241 TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG TCGACGGATC
3301 GGGAGATCTC CCGATCCCCT ATGGTGCACT CTCAGTACAA TCTGCTCTGA TGCCGCATAG
3361 TTAAGCCAGT ATCTGCTCCC TGCTTGTGTG TTGGAGGTCG CTGAGTAGTG CGCGAGCAAA
3421 ATTTAAGCTA CAACAAGGCA AGGCTTGACC GACAATTGCA TGAAGAATCT GCTTAGGGTT
3481 AGGCGTTTTG CGCTGCTTCG CGATGTACGG GCCAGATATA CGCGTTGACA TTGATTATTG
3541 ACTAGTTATT AATAGTAATC AATTACGGGG TCATTAGTTC ATAGCCCATA TATGGAGTTC
3601 CGCGTTACAT AACTTACGGT AAATGGCCCG CCTGGCTGAC CGCCCAACGA CCCCCGCCCA
3661 TTGACGTCAA TAATGACGTA TGTTCCCATA GTAACGCCAA TAGGGACTTT CCATTGACGT
3721 CAATGGGTGG AGTATTTACG GTAAACTGCC CACTTGGCAG TACATCAAGT GTATCATATG
3781 CCAAGTACGC CCCCTATTGA CGTCAATGAC GGTAAATGGC CCGCCTGGCA TTATGCCCAG
3841 TACATGACCT TATGGGACTT TCCTACTTGG CAGTACATCT ACGTATTAGT CATCGCTATT
3901 ACCATGGTGA TGCGGTTTTG GCAGTACATC AATGGGCGTG GATAGCGGTT TGACTCACGG
3961 GGATTTCCAA GTCTCCACCC CATTGACGTC AATGGGAGTT TGTTTTGGCA CCAAAATCAA
4021 CGGGACTTTC CAAAATGTCG TAACAACTCC GCCCCATTGA CGCAAATGGG CGGTAGGCGT
4081 GTACGGTGGG AGGTCTATAT AAGCAGCGCG TTTTGCCTGT ACTGGGTCTC TCTGGTTAGA
4141 CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA AGCCTCAATA
4201 AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT CTGGTAACTA
4261 GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG CCCGAACAGG
4321 GACTTGAAAG CGAAAGGGAA ACCAGAGGAG CTCTCTCGAC GCAGGACTCG GCTTGCTGAA
4381 GCGCGCACGG CAAGAGGCGA GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT TTTGACTAGC
4441 GGAGGCTAGA AGGAGAGAGA TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG GAGAATTAGA
4501 TCGCGATGGG AAAAAATTCG GTTAAGGCCA GGGGGAAAGA AAAAATATAA ATTAAAACAT
4561 ATAGTATGGG CAAGCAGGGA GCTAGAACGA TTCGCAGTTA ATCCTGGCCT GTTAGAAACA
4621 TCAGAAGGCT GTAGACAAAT ACTGGGACAG CTACAACCAT CCCTTCAGAC AGGATCAGAA
4681 GAACTTAGAT CATTATATAA TACAGTAGCA ACCCTCTATT GTGTGCATCA AAGGATAGAG
4741 ATAAAAGACA CCAAGGAAGC TTTAGACAAG ATAGAGGAAG AGCAAAACAA AAGTAAGACC
4801 ACCGCACAGC AAGCGGCCGC TGATCTTCAG ACCTGGAGGA GGAGATATGA GGGACAATTG
4861 GAGAAGTGAA TTATATAAAT ATAAAGTAGT AAAAATTGAA CCATTAGGAG TAGCACCCAC
4921 CAAGGCAAAG AGAAGAGTGG TGCAGAGAGA AAAAAGAGCA GTGGGAATAG GAGCTTTGTT
4981 CCTTGGGTTC TTGGGAGCAG CAGGAAGCAC TATGGGCGCA GCGTCAATGA CGCTGACGGT
5041 ACAGGCCAGA CAATTATTGT CTGGTATAGT GCAGCAGCAG AACAATTTGC TGAGGGCTAT
5101 TGAGGCGCAA CAGCATCTGT TGCAACTCAC AGTCTGGGGC ATCAAGCAGC TCCAGGCAAG
5161 AATCCTGGCT GTGGAAAGAT ACCTAAAGGA TCAACAGCTC CTGGGGATTT GGGGTTGCTC
5221 TGGAAAACTC ATTTGCACCA CTGCTGTGCC TTGGAATGCT AGTTGGAGTA ATAAATCTCT
5281 GGAACAGATT TGGAATCACA CGACCTGGAT GGAGTGGGAC AGAGAAATTA ACAATTACAC
5341 AAGCTTAATA CACTCCTTAA TTGAAGAATC GCAAAACCAG CAAGAAAAGA ATGAACAAGA
5401 ATTATTGGAA TTAGATAAAT GGGCAAGTTT GTGGAATTGG TTTAACATAA CAAATTGGCT
5461 GTGGTATATA AAATTATTCA TAATGATAGT AGGAGGCTTG GTAGGTTTAA GAATAGTTTT
5521 TGCTGTACTT TCTATAGTGA ATAGAGTTAG GCAGGGATAT TCACCATTAT CGTTTCAGAC
5581 CCACCTCCCA ACCCCGAGGG GACCCGACAG GCCCGAAGGA ATAGAAGAAG AAGGTGGAGA
5641 GAGAGACAGA GACAGATCCA TTCGATTAGT GAACGGATCG GCACTGCGTG CGCCAATTCT
5701 GCAGACAAAT GGCAGTATTC ATCCACAATT TTAAAAGAAA AGGGGGGATT GGGGGGTACA
5761 GTGCAGGGGA AAGAATAGTA GACATAATAG CAACAGACAT ACAAACTAAA GAATTACAAA
5821 AACAAATTAC AAAAATTCAA AATTTTCGGG TTTATTACAG GGACAGCAGA GATCCAGTTT
5881 GGTTAATTAG CTAGCGAGGG CCTATTTCCC ATGATTCCTT CATATTTGCA TATACGATAC
5941 AAGGCTGTTA GAGAGATAAT TGGAATTAAT TTGACTGTAA ACACAAAGAT ATTAGTACAA
6001 AATACGTGAC GTAGAAAGTA ATAATTTCTT GGGTAGTTTG CAGTTTTAAA ATTATGTTTT
6061 AAAATGGACT ATCATATGCT TACCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT
6121 ATCTTGTGGA AAGGACGAAA CACCGGAGAC GGGATACCGT CTCTGTTTTA GAGCTAGGCC
6181 AACATGAGGA TCACCCATGT CTGCAGGGCC TAGCAAGTTA AAATAAGGCT AGTCCGTTAT
6241 CAACTTGGCC AACATGAGGA TCACCCATGT CTGCAGGGCC AAGTGGCACC GAGTCGGTGC
6301 TTTTTTTGGA TCCTGCAAAG ATGGATAAAG TTTTAAACAG AGAGGAATCT TTGCAGCTAA
6361 TGGACCTTCT AGGTCTTGAA AGGAGTGGGA ATTGGCTCCG GTGCCCGTCA GTGGGCAGAG
6421 CGCACATCGC CCACAGTCCC CGAGAAGTTG GGGGGAGGGG TCGGCAATTG AACCGGTGCC
6481 TAGAGAAGGT GGCGCGGGGT AAACTGGGAA AGTGATGTCG TGTACTGGCT CCGCCTTTTT
6541 CCCGAGGGTG GGGGAGAACC GTATATAAGT GCAGTAGTCG CCGTGAACGT TCTTTTTCGC
6601 AACGGGTTTG CCGCCAGAAC ACAGGTAAGT GCCGTGTGTG GTTCCCGCGG GCCTGGCCTC
6661 TTTACGGGTT ATGGCCCTTG CGTGCCTTGA ATTACTTCCA CCTGGCTGCA GTACGTGATT
6721 CTTGATCCCG AGCTTCGGGT TGGAAGTGGG TGGGAGAGTT CGAGGCCTTG CGCTTAAGGA
6781 GCCCCTTCGC CTCGTGCTTG AGTTGAGGCC TGGCCTGGGC GCTGGGGCCG CCGCGTGCGA
6841 ATCTGGTGGC ACCTTCGCGC CTGTCTCGCT GCTTTCGATA AGTCTCTAGC CATTTAAAAT
6901 TTTTGATGAC CTGCTGCGAC GCTTTTTTTC TGGCAAGATA GTCTTGTAAA TGCGGGCCAA
6961 GATCTGCACA CTGGTATTTC GGTTTTTGGG GCCGCGGGCG GCGACGGGGC CCGTGCGTCC
7021 CAGCGCACAT GTTCGGCGAG GCGGGGCCTG CGAGCGCGGC CACCGAGAAT CGGACGGGGG
7081 TAGTCTCAAG CTGGCCGGCC TGCTCTGGTG CCTGGCCTCG CGCCGCCGTG TATCGCCCCG
7141 CCCTGGGCGG CAAGGCTGGC CCGGTCGGCA CCAGTTGCGT GAGCGGAAAG ATGGCCGCTT
7201 CCCGGCCCTG CTGCAGGGAG CTCAAAATGA AGGACGCGGC GCTCGGGAGA GCGGGCGGGT
7261 GAGTCACCCA CACAAAGGAA AAGGGCCTTT CCGTCCTCAG CCGTCGCTTC ATGTGACTCC
7321 ACGGAGTACC GGGCGCCGTC CAGGCACCTC GATTAGTTCT CGAGCTTTTG GAGTACGTCG
7381 TCTTTAGGTT GGGGGGAGGG GTTTTATGCG ATGGAGTTTC CCCACACTGA GTGGGTGGAG
7441 ACTGAAGTTA GGCCAGCTTG GCACTTGATG TAATTCTCCT TGGAATTTGC CCTTTTTGAG
7501 TTTGGATCTT GGTTCATTCT CAAGCCTCAG ACAGTGGTTC AAAGTTTTTT TCTTCCATTT
7561 CAGGTGTCGT GATGTACAAT GGCCAAGTTG ACCAGTGCCG TTCCGGTGCT CACCGCGCGC
7621 GACGTCGCCG GAGCGGTCGA GTTCTGGACC GACCGGCTCG GGTTCTCCCG GGACTTCGTG
7681 GAGGACGACT TCGCCGGTGT GGTCCGGGAC GACGTGACCC TGTTCATCAG CGCGGTCCAG
7741 GACCAGGTGG TGCCGGACAA CACCCTGGCC TGGGTGTGGG TGCGCGGCCT GGACGAGCTG
7801 TACGCCGAGT GGTCGGAGGT CGTGTCCACG AACTTCCGGG ACGCCTCCGG GCCGGCCATG
7861 ACCGAGATCG GCGAGCAGCC GTGGGGGCGG GAGTTCGCCC TGCGCGACCC GGCCGGCAAC
7921 TGCGTGCACT TCGTGGCCGA GGAGCAGGAC TGAGAATTCG ATATCAAGCT TATCGGTAAT
7981 CAACCTCTGG ATTACAAAAT TTGTGAAAGA TTGACTGGTA TTCTTAACTA TGTTGCTCCT
8041 TTTACGCTAT GTGGATACGC TGCTTTAATG CCTTTGTATC ATGCTATTGC TTCCCGTATG
8101 GCTTTCATTT TCTCCTCCTT GTATAAATCC TGGTTGCTGT CTCTTTATGA GGAGTTGTGG
8161 CCCGTTGTCA GGCAACGTGG CGTGGTGTGC ACTGTGTTTG CTGACGCAAC CCCCACTGGT
8221 TGGGGCATTG CCACCACCTG TCAGCTCCTT TCCGGGACTT TCGCTTTCCC CCTCCCTATT
8281 GCCACGGCGG AACTCATCGC CGCCTGCCTT GCCCGCTGCT GGACAGGGGC TCGGCTGTTG
8341 GGCACTGACA ATTCCGTGGT GTTGTCGGGG AAATCATCGT CCTTTCCTTG GCTGCTCGCC
8401 TGTGTTGCCA CCTGGATTCT GCGCGGGACG TCCTTCTGCT ACGTCCCTTC GGCCCTCAAT
8461 CCAGCGGACC TTCCTTCCCG CGGCCTGCTG CCGGCTCTGC GGCCTCTTCC GCGTCTTCGC
8521 CTTCGCCCTC AGACGAGTCG GATCTCCCTT TGGGCCGCCT CCCCGCATCG ATACCGTCGA
8581 CCTCGAGACC TAGAAAAACA TGGAGCAATC ACAAGTAGCA ATACAGCAGC TACCAATGCT
8641 GATTGTGCCT GGCTAGAAGC ACAAGAGGAG GAGGAGGTGG GTTTTCCAGT CACACCTCAG
8701 GTACCTTTAA GACCAATGAC TTACAAGGCA GCTGTAGATC TTAGCCACTT TTTAAAAGAA
8761 AAGGGGGGAC TGGAAGGGCT AATTCACTCC CAACGAAGAC AAGATATCCT TGATCTGTGG
8821 ATCTACCACA CACAAGGCTA CTTCCCTGAT TGGCAGAACT ACACACCAGG GCCAGGGATC
8881 AGATATCCAC TGACCTTTGG ATGGTGCTAC AAGCTAGTAC CAGTTGAGCA AGAGAAGGTA
8941 GAAGAAGCCA ATGAAGGAGA GAACACCCGC TTGTTACACC CTGTGAGCCT GCATGGGATG
9001 GATGACCCGG AGAGAGAAGT ATTAGAGTGG AGGTTTGACA GCCGCCTAGC ATTTCATCAC
9061 ATGGCCCGAG AGCTGCATCC GGACTGTACT GGGTCTCTCT GGTTAGACCA GATCTGAGCC
9121 TGGGAGCTCT CTGGCTAACT AGGGAACCCA CTGCTTAAGC CTCAATAAAG CTTGCCTTGA
9181 GTGCTTCAAG TAGTGTGTGC CCGTCTGTTG TGTGACTCTG GTAACTAGAG ATCCCTCAGA
9241 CCCTTTTAGT CAGTGTGGAA AATCTCTAGC AGGGCCCGTT TAAACCCGCT GATCAGCCTC
9301 GACTGTGCCT TCTAGTTGCC AGCCATCTGT TGTTTGCCCC TCCCCCGTGC CTTCCTTGAC
9361 CCTGGAAGGT GCCACTCCCA CTGTCCTTTC CTAATAAAAT GAGGAAATTG CATCGCATTG
9421 TCTGAGTAGG TGTCATTCTA TTCTGGGGGG TGGGGTGGGG CAGGACAGCA AGGGGGAGGA
9481 TTGGGAAGAC AATAGCAGGC ATGCTGGGGA TGCGGTGGGC TCTATGGCTT CTGAGGCGGA
9541 AAGAACCAGC TGGGGCTCTA GGGGGTATCC CCACGCGCCC TGTAGCGGCG CATTAAGCGC
9601 GGCGGGTGTG GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC
9661 TCCTTTCGCT TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT
9721 AAATCGGGGG CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA
9781 ACTTGATTAG GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC
9841 TTTGACGTTG GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT
9901 CAACCCTATC TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG
9961 GTTAAAAAAT GAGCTGATTT AACAAAAATT TAACGCG
//
