| 质粒名称: | mCh-alpha-tubulin |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 49149 |
| 存储实验室: | Gia Voeltz |
|
载体骨架基本信息 |
|
| 载体名称: | EYFP-alpha-tubulin |
| 载体出品公司: | Takara Bio Inc |
| 载体抗性: | 卡那霉素 |
| 空载体大小: | -- |
| 完整质粒大小: | -- |
| 载体修饰: | mCherry 取代 EYFP |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | 新霉素（G418） |
| 高拷贝/低拷贝: | -- |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | alpha tubulin,tubulin, alpha 1b,α tubulin,TUBA1B |
| 物种来源: | H. sapiens (human) |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | CMV |
| 标签/融合蛋白: | mCherry（主干上的N终端） |
| 克隆方法: | -- |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | mCherry-F (ccccgtaatgcagaagaaga) |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | mCh-alpha-tubulin was a gift from Gia Voeltz (Addgene plasmid # 49149 ; http://n2t.net/addgene:49149 ; RRID:Addgene_49149) |
| 参考文献部分: | ER sliding dynamics and ER-mitochondrial contacts occur on acetylated microtubules. Friedman JR, Webster BM, Mastronarde DN, Verhey KJ, Voeltz GK. J Cell Biol. 2010 Aug 9;190(3):363-75. doi: 10.1083/jcb.200911024. 10.1083/jcb.200911024 PubMed 20696706 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS mCh-alpha-tubulin 6036 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION expression of alpha-tubulin fused to mCherry. Used for visualization
of microtubules..
ACCESSION .
VERSION .
KEYWORDS mCh-alpha-tubulin
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6036)
AUTHORS Friedman JR, Webster BM, Mastronarde DN, Verhey KJ, Voeltz GK
TITLE ER sliding dynamics and ER-mitochondrial contacts occur on
acetylated microtubules.
JOURNAL J Cell Biol. 2010 Aug 9;190(3):363-75. doi: 10.1083/jcb.200911024.
PUBMED 20696706
REFERENCE 2 (bases 1 to 6036)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..6036
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 68..371
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 372..575
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 526..546
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
regulatory 614..623
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 620..1327
/codon_start=1
/product="monomeric derivative of DsRed fluorescent protein
(Shaner et al., 2004)"
/label=mCherry
/note="mammalian codon-optimized"
/translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
EGRHSTGGMDELYK"
primer_bind complement(765..783)
/label=mCherry-R
/note="mCherry, reverse primer"
primer_bind 1033..1052
/label=mCherry-F
/note="mCherry, forward primer"
polyA_signal 2831..2952
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(2868..2887)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 2922..2941
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
rep_origin complement(2959..3414)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(3096..3117)
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind 3308..3327
/label=F1ori-R
/note="F1 origin, reverse primer"
promoter 3441..3545
/gene="bla"
/label=AmpR promoter
promoter 3547..3904
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
primer_bind complement(3570..3590)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
rep_origin 3755..3890
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 3817..3836
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
CDS 3939..4733
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(3993..4012)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 4603..4622
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
primer_bind complement(4921..4940)
/label=TK-pA-R
/note="Thymidine kinase polyA, reverse primer"
polyA_signal 4965..5012
/label=HSV TK poly(A) signal
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
rep_origin 5341..5929
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 5830..5849
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
ORIGIN
1 catgcattag ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg
61 agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc
121 gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt
181 gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc
241 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
301 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
361 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
421 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
481 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
541 ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct
601 agcgctaccg gtcgccacca tggtgagcaa gggcgaggag gataacatgg ccatcatcaa
661 ggagttcatg cgcttcaagg tgcacatgga gggctccgtg aacggccacg agttcgagat
721 cgagggcgag ggcgagggcc gcccctacga gggcacccag accgccaagc tgaaggtgac
781 caagggtggc cccctgccct tcgcctggga catcctgtcc cctcagttca tgtacggctc
841 caaggcctac gtgaagcacc ccgccgacat ccccgactac ttgaagctgt ccttccccga
901 gggcttcaag tgggagcgcg tgatgaactt cgaggacggc ggcgtggtga ccgtgaccca
961 ggactcctcc ctgcaggacg gcgagttcat ctacaaggtg aagctgcgcg gcaccaactt
1021 cccctccgac ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct cctccgagcg
1081 gatgtacccc gaggacggcg ccctgaaggg cgagatcaag cagaggctga agctgaagga
1141 cggcggccac tacgacgctg aggtcaagac cacctacaag gccaagaagc ccgtgcagct
1201 gcccggcgcc tacaacgtca acatcaagtt ggacatcacc tcccacaacg aggactacac
1261 catcgtggaa cagtacgaac gcgccgaggg ccgccactcc accggcggca tggacgagct
1321 gtacaagtcc ggactcagat ctcgagtgcg tgagtgcatc tccatccacg ttggccaggc
1381 tggtgtccag attggcaatg cctgctggga gctctactgc ctggaacacg gcatccagcc
1441 cgatggccag atgccaagtg acaagaccat tgggggagga gatgactcct tcaacacctt
1501 cttcagtgag acgggcgctg gcaagcacgt gccccgggct gtgtttgtag acttggaacc
1561 cacagtcatt gatgaagttc gcactggcac ctaccgccag ctcttccacc ctgagcagct
1621 catcacaggc aaggaagatg ctgccaataa ctatgcccga gggcactaca ccattggcaa
1681 ggagatcatt gaccttgtgt tggaccgaat tcgcaagctg gctgaccagt gcaccggtct
1741 tcagggcttc ttggttttcc acagctttgg tgggggaact ggttctgggt tcacctccct
1801 gctcatggaa cgtctctcag ttgattatgg caagaagtcc aagctggagt tctccattta
1861 cccagcaccc caggtttcca cagctgtagt tgagccctac aactccatcc tcaccaccca
1921 caccaccctg gagcactctg attgtgcctt catggtagac aatgaggcca tctatgacat
1981 ctgtcgtaga aacctcgata tcgagcgccc aacctacact aaccttaacc gccttattag
2041 ccagattgtg tcctccatca ctgcttccct gagatttgat ggagccctga atgttgacct
2101 gacagaattc cagaccaacc tggtgcccta cccccgcatc cacttccctc tggccacata
2161 tgcccctgtc atctctgctg agaaagccta ccatgaacag ctttctgtag cagagatcac
2221 caatgcttgc tttgagccag ccaaccagat ggtgaaatgt gaccctcgcc atggtaaata
2281 catggcttgc tgcctgttgt accgtggtga cgtggttccc aaagatgtca atgctgccat
2341 tgccaccatc aaaaccaagc gcagcatcca gtttgtggat tggtgcccca ctggcttcaa
2401 ggttggcatc aactaccagc ctcccactgt ggtgcctggt ggagacctgg ccaaggtaca
2461 gagagctgtg tgcatgctga gcaacaccac agccattgct gaggcctggg ctcgcctgga
2521 ccacaagttt gacctgatgt atgccaagcg tgcctttgtt cactggtacg tgggtgaggg
2581 gatggaggaa ggcgagtttt cagaggcccg tgaagatatg gctgcccttg agaaggatta
2641 tgaggaggtt ggtgtggatt ctgttgaagg agagggtgag gaagaaggag aggaatacta
2701 aggatccacc ggatctagat aactgatcat aatcagccat accacatttg tagaggtttt
2761 acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat
2821 tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
2881 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
2941 caatgtatct taaggcgtaa attgtaagcg ttaatatttt gttaaaattc gcgttaaatt
3001 tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat
3061 caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat
3121 taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac
3181 tacgtgaacc atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc
3241 ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga
3301 gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca
3361 cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtcaggtg
3421 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
3481 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
3541 agagtcctga ggcggaaaga accagctgtg gaatgtgtgt cagttagggt gtggaaagtc
3601 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag
3661 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
3721 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
3781 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
3841 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg
3901 caaagatcga tcaagagaca ggatgaggat cgtttcgcat gattgaacaa gatggattgc
3961 acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga
4021 caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt
4081 ttgtcaagac cgacctgtcc ggtgccctga atgaactgca agacgaggca gcgcggctat
4141 cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg
4201 gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg
4261 ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc
4321 cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga
4381 tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag
4441 ccgaactgtt cgccaggctc aaggcgagca tgcccgacgg cgaggatctc gtcgtgaccc
4501 atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg
4561 actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata
4621 ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg
4681 ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagcgggac
4741 tctggggttc gaaatgaccg accaagcgac gcccaacctg ccatcacgag atttcgattc
4801 caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat
4861 gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccctaggg ggaggctaac
4921 tgaaacacgg aaggagacaa taccggaagg aacccgcgct atgacggcaa taaaaagaca
4981 gaataaaacg cacggtgttg ggtcgtttgt tcataaacgc ggggttcggt cccagggctg
5041 gcactctgtc gataccccac cgagacccca ttggggccaa tacgcccgcg tttcttcctt
5101 ttccccaccc caccccccaa gttcgggtga aggcccaggg ctcgcagcca acgtcggggc
5161 ggcaggccct gccatagcct caggttactc atatatactt tagattgatt taaaacttca
5221 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
5281 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
5341 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
5401 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
5461 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
5521 caagaactct gtatcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
5581 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
5641 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
5701 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
5761 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
5821 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
5881 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
5941 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
6001 gttatcccct gattctgtgg ataaccgtat taccgc
//
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