| 质粒名称: | mEos3.2-C1 |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 54550 |
| 存储实验室: | Michael Davidson, Tao Xu |
|
载体骨架基本信息 |
|
| 载体名称: | mEos3.2-C1 |
| 载体出品公司: | -- |
| 载体抗性: | 卡那霉素 |
| 空载体大小: | 4750 bp |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | 新霉素（G418） |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | -- |
| 物种来源: | -- |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | mEos3.2（主干网上的N终端） |
| 克隆方法: | -- |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | mEos3.2-C1 was a gift from Michael Davidson & Tao Xu (Addgene plasmid # 54550 ; http://n2t.net/addgene:54550 ; RRID:Addgene_54550) |
| 参考文献部分: | Rational design of true monomeric and bright photoactivatable fluorescent proteins. Zhang M, Chang H, Zhang Y, Yu J, Wu L, Ji W, Chen J, Liu B, Lu J, Liu Y, Zhang J, Xu P, Xu T. Nat Methods. 2012 May 13;9(7):727-9. doi: 10.1038/nmeth.2021. 10.1038/nmeth.2021 PubMed 22581370 |
买家导航
mEos3.2C1载体基本信息
mEos3.2C1载体图谱质粒图谱和多克隆位点信息
mEos3.2C1载体简介
mEos3.2C1载体序列
LOCUS mEos3.2-C1 4692 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION Localization: C1 Cloning Vector, Excitation: 507 / 572, Emission:
516 / 580.
ACCESSION .
VERSION .
KEYWORDS mEos3.2-C1
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4692)
TITLE Michael Davidson Empty Backbones
REFERENCE 2 (bases 1 to 4692)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..4692
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 16..319
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 320..523
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 474..494
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
CDS 568..1245
/codon_start=1
/product="improved monomeric variant of green-to-red
photoswitchable fluorescent protein EosFP (Zhang et al.,
2012)"
/label=mEos3.2
/translation="MSAIKPDMKIKLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
EGGPLPFAFDILTTAFHYGNRVFAKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICNAR
NDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIEMALLL
EGNAHYRCDFRTTYKAKEKGVKLPGAHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
NARR"
misc_feature 1246..1311
/label=MCS
/note="multiple cloning site"
polyA_signal 1435..1556
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(1472..1491)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 1526..1545
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
rep_origin complement(1563..2018)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(1700..1721)
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind 1912..1931
/label=F1ori-R
/note="F1 origin, reverse primer"
promoter 2045..2149
/gene="bla"
/label=AmpR promoter
promoter 2151..2508
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
primer_bind complement(2174..2194)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
rep_origin 2359..2494
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 2421..2440
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
CDS 2543..3337
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(2597..2616)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 3207..3226
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
primer_bind complement(3525..3544)
/label=TK-pA-R
/note="Thymidine kinase polyA, reverse primer"
polyA_signal 3569..3616
/label=HSV TK poly(A) signal
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
rep_origin 3945..4533
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 4434..4453
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
ORIGIN
1 atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa
61 cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac
121 tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca
181 agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg
241 gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt
301 agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg
361 gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg
421 gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat
481 gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctggtttag tgaaccgtca
541 gatccgctag cgctaccggt cgccaccatg agtgcgatta agccagacat gaagatcaaa
601 ctccgtatgg aaggcaacgt aaacgggcac cactttgtga tcgacggaga tggtacaggc
661 aagccttttg agggaaaaca gagtatggat cttgaagtca aagagggcgg acctctgcct
721 tttgcctttg atatcctgac cactgcattc cattacggca acagggtatt cgccaaatat
781 ccagacaaca tacaagacta ttttaagcag tcgtttccta aggggtattc gtgggaacga
841 agcttgactt tcgaagacgg gggcatttgc aacgccagaa acgacataac aatggaaggg
901 gacactttct ataataaagt tcgattttat ggtaccaact ttcccgccaa tggtccagtt
961 atgcagaaga agacgctgaa atgggagccc tccactgaga aaatgtatgt gcgtgatgga
1021 gtgctgacgg gtgatattga gatggctttg ttgcttgaag gaaatgccca ttaccgatgt
1081 gacttcagaa ctacttacaa agctaaggag aagggtgtca agttaccagg cgcccacttt
1141 gtggaccact gcattgagat tttaagccat gacaaagatt acaacaaggt taagctgtat
1201 gagcatgctg ttgctcattc tggattgcct gacaatgcca gacgatccgg actcagatct
1261 cgagctcaag cttcgaattc tgcagtcgac ggtaccgcgg gcccgggatc caccggatct
1321 agataactga tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac
1381 ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg
1441 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
1501 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttaacgc
1561 gtaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
1621 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
1681 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
1741 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
1801 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
1861 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
1921 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
1981 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtca ggtggcactt ttcggggaaa
2041 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
2101 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagtc ctgaggcgga
2161 aagaaccagc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca
2221 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca
2281 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc
2341 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
2401 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta
2461 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaga tcgatcaaga
2521 gacaggatga ggatcgtttc gcatgattga acaagatgga ttgcacgcag gttctccggc
2581 cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga
2641 tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct
2701 gtccggtgcc ctgaatgaac tgcaagacga ggcagcgcgg ctatcgtggc tggccacgac
2761 gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct
2821 attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt
2881 atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt
2941 cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt
3001 cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag
3061 gctcaaggcg agcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt
3121 gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg
3181 tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg
3241 cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg
3301 catcgccttc tatcgccttc ttgacgagtt cttctgagcg ggactctggg gttcgaaatg
3361 accgaccaag cgacgcccaa cctgccatca cgagatttcg attccaccgc cgccttctat
3421 gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg
3481 gatctcatgc tggagttctt cgcccaccct agggggaggc taactgaaac acggaaggag
3541 acaataccgg aaggaacccg cgctatgacg gcaataaaaa gacagaataa aacgcacggt
3601 gttgggtcgt ttgttcataa acgcggggtt cggtcccagg gctggcactc tgtcgatacc
3661 ccaccgagac cccattgggg ccaatacgcc cgcgtttctt ccttttcccc accccacccc
3721 ccaagttcgg gtgaaggccc agggctcgca gccaacgtcg gggcggcagg ccctgccata
3781 gcctcaggtt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
3841 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
3901 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
3961 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
4021 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
4081 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
4141 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
4201 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
4261 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
4321 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
4381 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
4441 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
4501 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
4561 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
4621 gtggataacc gtattaccgc catgcattag ttattaatag taatcaatta cggggtcatt
4681 agttcatagc cc
//
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