| 质粒名称: | mKeima-Red-C1 |
|---|---|
| 出品公司： | addgene |
| 目录编号: | 54546 |
| 存储实验室: | Michael Davidson |
|
载体骨架基本信息 |
|
| 载体名称: | mKeima-Red-C1 |
| 空载体大小: | 4750 bp |
| 完整质粒大小: | 4680 bp |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | 新霉素（G418） |
| 载体抗性: | 卡那霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | 未知 |
| 标签/融合蛋白: | mKeima-Red (N terminal on backbone) |
|
文献引用方法 |
|
| 材料和方法部分: | mKeima-Red-C1 was a gift from Michael Davidson (Addgene plasmid # 54546 ; http://n2t.net/addgene:54546 ; RRID:Addgene_54546) |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS mKeima-Red-C1 4680 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Localization: C1 Cloning Vector, Excitation: 440, Emission: 620.
ACCESSION .
VERSION .
KEYWORDS mKeima-Red-C1
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4680)
TITLE Michael Davidson Empty Backbones
REFERENCE 2 (bases 1 to 4680)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..4680
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 105..408
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 409..612
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 563..583
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
regulatory 651..660
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 657..1322
/codon_start=1
/product="monomeric Keima-Red fluorescent protein, also
known as mKeima"
/label=mKeima-Red
/translation="MVSVIAKQMTYKVYMSGTVNGHYFEVEGDGKGKPYEGEQTVKLTV
TKGGPLPFAWDILSPQLQYGSIPFTKYPEDIPDYFKQSFPEGYTWERSMNFEDGAVCTV
SNDSSIQGNCFIYNVKISGENFPPNGPVMQKKTQGWEPSTERLFARDGMLIGNDYMALK
LEGGGHYLCEFKSTYKAKKPVRMPGRHEIDRKLDVTSHNRDYTSVEQCEIAIARHSLLG
"
misc_feature 1323..1388
/label=MCS
/note="multiple cloning site"
polyA_signal 1512..1633
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(1549..1568)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 1603..1622
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
rep_origin complement(1640..2095)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(1777..1798)
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind 1989..2008
/label=F1ori-R
/note="F1 origin, reverse primer"
promoter 2122..2226
/gene="bla"
/label=AmpR promoter
promoter 2228..2585
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
primer_bind complement(2251..2271)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
rep_origin 2436..2571
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 2498..2517
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
CDS 2620..3414
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(2674..2693)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 3284..3303
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
primer_bind complement(3602..3621)
/label=TK-pA-R
/note="Thymidine kinase polyA, reverse primer"
polyA_signal 3646..3693
/label=HSV TK poly(A) signal
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
rep_origin 4022..4610
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 4511..4530
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
ORIGIN
1 cgttatcccc tgattctgtg gataaccgta ttaccgccat gcattagtta ttaatagtaa
61 tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg
121 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg
181 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta
241 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt
301 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac
361 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt
421 tggcagtaca tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac
481 cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt
541 cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat
601 ataagcagag ctggtttagt gaaccgtcag atccgctagc gctaccggtc gccaccatgg
661 tgagtgtgat cgctaaacaa atgacctaca aggtttatat gtcaggcacg gtcaatggac
721 actactttga ggtcgaaggc gatggaaaag gaaagcctta cgagggagag cagacagtaa
781 agctcactgt caccaagggt ggacctctgc catttgcttg ggatatttta tcaccacagc
841 ttcagtacgg aagcatacca ttcaccaagt accctgaaga catccctgat tatttcaagc
901 agtcattccc tgagggatat acatgggaga ggagcatgaa ctttgaagat ggtgcagtgt
961 gtactgtcag caatgattcc agcatccaag gcaactgttt catctacaat gtcaaaatct
1021 ctggtgagaa ctttcctccc aatggacctg ttatgcagaa gaagacacag ggctgggaac
1081 ccagcactga gcgtctcttt gcacgagatg gaatgctgat aggaaacgat tatatggctc
1141 tgaagttgga aggaggtggt cactatttgt gtgaatttaa atctacttac aaggcaaaga
1201 agcctgtgag gatgccaggg cgccacgaga ttgaccgcaa actggatgta accagtcaca
1261 acagggatta cacatctgtt gagcagtgtg aaatagccat tgcacgccac tctttgctcg
1321 gttccggact cagatctcga gctcaagctt cgaattctgc agtcgacggt accgcgggcc
1381 cgggatccac cggatctaga taactgatca taatcagcca taccacattt gtagaggttt
1441 tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa
1501 ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
1561 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
1621 tcaatgtatc ttaacgcgta aattgtaagc gttaatattt tgttaaaatt cgcgttaaat
1681 ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat cccttataaa
1741 tcaaaagaat agaccgagat agggttgagt gttgttccag tttggaacaa gagtccacta
1801 ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca
1861 ctacgtgaac catcacccta atcaagtttt ttggggtcga ggtgccgtaa agcactaaat
1921 cggaacccta aagggagccc ccgatttaga gcttgacggg gaaagccggc gaacgtggcg
1981 agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc
2041 acgctgcgcg taaccaccac acccgccgcg cttaatgcgc cgctacaggg cgcgtcaggt
2101 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
2161 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
2221 aagagtcctg aggcggaaag aaccagctgt ggaatgtgtg tcagttaggg tgtggaaagt
2281 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
2341 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
2401 agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt
2461 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg
2521 cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt
2581 gcaaagatcg atcaagagac aggatgagga tcgtttcgca tgattgaaca agatggattg
2641 cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag
2701 acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt
2761 tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aagacgaggc agcgcggcta
2821 tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg
2881 ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt
2941 gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat
3001 ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg
3061 atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca
3121 gccgaactgt tcgccaggct caaggcgagc atgcccgacg gcgaggatct cgtcgtgacc
3181 catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc
3241 gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat
3301 attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc
3361 gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgagcggga
3421 ctctggggtt cgaaatgacc gaccaagcga cgcccaacct gccatcacga gatttcgatt
3481 ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga
3541 tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccctagg gggaggctaa
3601 ctgaaacacg gaaggagaca ataccggaag gaacccgcgc tatgacggca ataaaaagac
3661 agaataaaac gcacggtgtt gggtcgtttg ttcataaacg cggggttcgg tcccagggct
3721 ggcactctgt cgatacccca ccgagacccc attggggcca atacgcccgc gtttcttcct
3781 tttccccacc ccacccccca agttcgggtg aaggcccagg gctcgcagcc aacgtcgggg
3841 cggcaggccc tgccatagcc tcaggttact catatatact ttagattgat ttaaaacttc
3901 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
3961 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
4021 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
4081 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
4141 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
4201 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
4261 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
4321 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
4381 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
4441 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
4501 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
4561 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
4621 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
//
载体图谱质粒图谱pdf版和相关资料下载
载体质粒应用举例
- 上一篇：pET42b-ABE7.10
- 下一篇：pF146 pSOD1WTAcGFP1
