LOCUS p16sheLL 10831 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS p16sheLL
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 10831)
AUTHORS Buck CB, Thompson CD, Roberts JN, Muller M, Lowy DR, Schiller JT
TITLE Carrageenan is a potent inhibitor of papillomavirus infection.
JOURNAL PLoS Pathog. 2006 Jul;2(7):e69.
PUBMED 16839203
REFERENCE 2 (bases 1 to 10831)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..10831
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 83..462
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 463..666
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 617..637
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
protein_bind 668..686
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
protein_bind 689..707
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
primer_bind 717..741
/label=LNCX
/note="Human CMV promoter, forward primer"
promoter 840..858
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(2396..2412)
/label=KS primer
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(2397..2413)
/label=pBluescriptKS
/note="For pBluescript vector"
misc_feature 4566..5154
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(4619..4639)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(5037..5048)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
primer_bind complement(5191..5208)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 5197..5421
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 5467..5895
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(5554..5573)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 5764..5785
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind complement(5904..5924)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
CDS 7854..8222
/codon_start=1
/gene="Sh ble from Streptoalloteichus hindustanus"
/product="antibiotic-binding protein"
/label=BleoR
/note="confers resistance to bleomycin, phleomycin, and
Zeocin(TM)"
/translation="KLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDDVT
LFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGREF
ALRDPAGNCVHFVAEEQD"
polyA_signal 8352..8473
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(8389..8408)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 8443..8462
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind complement(8522..8538)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(8522..8538)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(8535..8557)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 8546..8562
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(8570..8600)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 8615..8636
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(8753..8770)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(8924..9512)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(9004..9023)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(9683..10543)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 10306..10325
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(10544..10648)
/gene="bla"
/label=AmpR promoter
primer_bind complement(10723..10742)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
ORIGIN
1 ttgaccgaca attgcatgaa gaatctgctt agggttaggc gttttgcgct gcttcgcgat
61 gtacgggcca gatatacgcg ttgacattga ttattgacta gttattaata gtaatcaatt
121 acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat
181 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt
241 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa
301 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc
361 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct
421 acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag
481 tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt
541 gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac
601 aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc
661 agagctctcc ctatcagtga tagagatctc cctatcagtg atagagatcg tcgacgagct
721 cgtttagtga accgtcagat cgcctggaga cgccatccac gctgttttga cctccataga
781 agacaccggg accgatccag cctccggact ctagcgttta aacttaaggc tagagtactt
841 aatacgactc actataggct agagccacca tgagcctgtg gctgcccagc gaggccaccg
901 tgtacctgcc ccccgtgccc gtgagcaagg tggtgagcac cgacgagtac gtggccagga
961 ccaacatcta ctaccacgcc ggcaccagca ggctgctggc cgtgggccac ccctacttcc
1021 ccatcaagaa gcccaacaac aacaagatcc tggtgcccaa ggtgagcggc ctgcagtaca
1081 gggtgttcag gatccacctg cccgacccca acaagttcgg cttccccgac accagcttct
1141 acaaccccga cacccagagg ctggtgtggg cctgcgtggg cgtggaggtg ggcaggggcc
1201 agcccctggg cgtgggcatc agcggccacc ccctgctgaa caagctggac gacaccgaga
1261 acgccagcgc ctacgccgcc aacgccggcg tggacaacag ggagtgcatc agcatggact
1321 acaagcagac ccagctgtgc ctgatcggct gcaagccccc catcggcgag cactggggca
1381 agggcagccc ctgcaccaac gtggccgtga accccggcga ctgccccccc ctggagctga
1441 tcaacaccgt gatccaggac ggcgacatgg tggacaccgg cttcggcgcc atggacttca
1501 ccaccctgca ggccaacaag agcgaggtgc ccctggacat ctgcaccagc atctgcaagt
1561 accccgacta catcaagatg gtgagcgagc cctacggcga cagcctgttc ttctacctga
1621 ggagggagca gatgttcgtg aggcacctgt tcaacagggc cggcgccgtg ggcgagaacg
1681 tgcccgacga cctgtacatc aagggcagcg gcagcaccgc caacctggcc agcagcaact
1741 acttccccac ccccagcggc agcatggtga ccagcgacgc ccagatcttc aacaagccct
1801 actggctgca gagggcccag ggccacaaca acggcatctg ctggggcaac cagctgttcg
1861 tgaccgtggt ggacaccacc aggagcacca acatgagcct gtgcgccgcc atcagcacca
1921 gcgagaccac ctacaagaac accaacttca aggagtacct gaggcacggc gaggagtacg
1981 acctgcagtt catcttccag ctgtgcaaga tcaccctgac cgccgacgtg atgacctaca
2041 tccacagcat gaacagcacc atcctggagg actggaactt cggcctgcag cccccccccg
2101 gcggcaccct ggaggacacc tacaggttcg tgaccagcca ggccatcgcc tgccagaagc
2161 acaccccccc cgcccccaag gaggaccccc tgaagaagta caccttctgg gaggtgaacc
2221 tgaaggagaa gttcagcgcc gacctggacc agttccccct gggcaggaag ttcctgctgc
2281 aggccggcct gaaggccaag cccaagttca ccctgggcaa gaggaaggcc acccccacca
2341 ccagcagcac cagcaccacc gccaagagga agaagaggaa gctgtgaaag cttatcgata
2401 ccgtcgacct cgacctgcag aagcttaaaa cagctctggg gttgtaccca ccccagaggc
2461 ccacgtggcg gctagtactc cggtattgcg gtacccttgt acgcctgttt tatactccct
2521 tcccgtaact tagacgcaca aaaccaagtt caatagaagg gggtacaaac cagtaccacc
2581 acgaacaagc acttctgttt ccccggtgat gtcgtataga ctgcttgcgt ggttgaaagc
2641 gacggatccg ttatccgctt atgtacttcg agaagcccag taccacctcg gaatcttcga
2701 tgcgttgcgc tcagcactca accccagagt gtagcttagg ctgatgagtc tggacatccc
2761 tcaccggtga cggtggtcca ggctgcgttg gcggcctacc tatggctaac gccatgggac
2821 gctagttgtg aacaaggtgt gaagagccta ttgagctaca taagaatcct ccggcccctg
2881 aatgcggcta atcccaacct cggagcaggt ggtcacaaac cagtgattgg cctgtcgtaa
2941 cgcgcaagtc cgtggcggaa ccgactactt tgggtgtccg tgtttccttt tattttattg
3001 tggctgctta tggtgacaat cacagattgt tatcataaag cgaattggat tgcggccgct
3061 ctagagccac catgaggcac aagaggagcg ccaagaggac caagagggcc agcgccaccc
3121 agctgtacaa gacctgcaag caggccggca cctgcccccc cgacatcatc cccaaggtgg
3181 agggcaagac catcgccgac cagatcctgc agtacggcag catgggcgtg ttcttcggcg
3241 gcctgggcat cggcaccggc agcggcaccg gcggcaggac cggctacatc cccctgggca
3301 ccaggccccc caccgccacc gacaccctgg cccccgtgag gccccccctg accgtggacc
3361 ccgtgggccc cagcgacccc agcatcgtga gcctggtgga ggagaccagc ttcatcgacg
3421 ccggcgcccc caccagcgtg cccagcatcc cccccgacgt gagcggcttc agcatcacca
3481 ccagcaccga caccaccccc gccatcctgg acatcaacaa caccgtgacc accgtgacca
3541 cccacaacaa ccccaccttc accgacccca gcgtgctgca gccccccacc cccgccgaga
3601 ccggcggcca cttcaccctg agcagcagca ccatcagcac ccacaactac gaggagatcc
3661 ccatggacac cttcatcgtg agcaccaacc ccaacaccgt gaccagcagc acccccatcc
3721 ccggcagcag gcccgtggcc aggctgggcc tgtacagcag gaccacccag caggtgaagg
3781 tggtggaccc cgccttcgtg accaccccca ccaagctgat cacctacgac aaccccgcct
3841 acgagggcat cgacgtggac aacaccctgt acttcagcag caacgacaac agcatcaaca
3901 tcgcccccga ccccgacttc ctggacatcg tggccctgca caggcccgcc ctgaccagca
3961 ggaggaccgg catcaggtac agcaggatcg gcaacaagca gaccctgagg accaggagcg
4021 gcaagagcat cggcgccaag gtgcactact actacgacct gagcaccatc gaccccgccg
4081 aggagatcga gctgcagacc atcaccccca gcacctacac caccaccagc cacgccgcca
4141 gccccaccag catcaacaac ggcctgtacg acatctacgc cgacgacttc atcaccgaca
4201 ccagcaccac ccccgtgccc agcgtgccca gcaccagcct gagcggctac atccccgcca
4261 acaccaccat ccccttcggt ggcgcctaca acatccccct ggtgagcggc cccgacatcc
4321 ccatcaacat caccgaccag gcccccagcc tgatccccat cgtgcccggc agcccccagt
4381 acaccatcat cgccgacgcc ggcgacttct acctgcaccc cagctactac atgctgagga
4441 agaggaggaa gaggctgccc tacttcttca gcgacgtgag cctggccgcc tgaaagcttt
4501 ttgaattctt tggatccact agtggatccc ccgggctgca ggaattcgat atcaagctta
4561 tcgataatca acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg
4621 ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt
4681 cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg
4741 agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc
4801 ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc
4861 tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc
4921 ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc
4981 tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg
5041 ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc
5101 gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcatcgat
5161 accgtcggcc cgtttaaacc cgctgatcag cctcgactgt gccttctagt tgccagccat
5221 ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc
5281 tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg
5341 ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg
5401 gggatgcggt gggctctatg gcttctgagg cggaaagaac cagctggggc tctagggggt
5461 atccccacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
5521 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
5581 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
5641 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
5701 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
5761 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg
5821 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa
5881 aatttaacgc gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg
5941 ctccccagca ggcagaagta tgcaaagcat gcagaattct atcaaatatt taaagaaaaa
6001 aaaattgtat caactttcta caatctcttt cagaagacag aagcagaggg aatacttcct
6061 aaatcattca actaggccag cattacctta ataccggaac tagaaaatga cattacaaga
6121 aaagaaaaca acagaccaat atctctcatg aacaaagata caaacatttt caacaaaata
6181 ttagcaaaaa gaatccaaga atgtatcaaa aaatatacac cacaaccaag tagaatttat
6241 tccagatatg taagggtggt tcaacgtttg aaaatcaatt aacgtaattt gtcccatcaa
6301 caggttaaag aagaaaatca catggtcata ttgatagaca cagaaaaagc atttgacaaa
6361 atttaacacc cattcatgat gcaatctctc agtaaactag gaatagagga aaacttcctc
6421 agcttgaatg taccttcctc tcaattttgc tatgaacctg aaactcctct taaaaaataa
6481 agtttttcat ttaaaaagaa aacaaaaaac atggaggagc gttgatgtat ctcattttag
6541 accaatcagc tatggatagt taggcgacag cacagatagc tgctgtactt ctgtttctgg
6601 caatgttcca gactacattt aaaaaatttt taattataga cttgtactta atgttcaaga
6661 aaaatatgaa aatggctttg ccgtgttaat gctactcttt tttaaaaaaa aactaaagtt
6721 caaactttat ttatatttca ttagtttttt agctactgtt ctttttctgt tctgggatct
6781 cattcagaat gccacattac atataattct catgtctcct tgggttcctc ttagttttga
6841 cagttcctca gacttttctt atttttgatg accttgacag ttttgaggag tactggttag
6901 atatagggta atggttttta aagtatattt gtcatgattt atactggggt aagggtttgg
6961 ggaggaagcc catggggtaa agtactgttc tcatcacatc atatcaaggt tatataccat
7021 caatattgcc acagatgtta cttagccttt taatatttct ctaatttagt gtatatgcaa
7081 tgatagttct ctgatttctg agattgagtt tctcatgtgt aatgattatt tagagtttct
7141 ctttcatctg ttcaaatttt tgtctagttt tattttttac tgatttgtaa gacttctttt
7201 tataatctgc atattacaat tctctttact ggggtgttgc aaatattttc tgtcattcta
7261 tggcctgact tttcttaatg gttttttaat tttaaaaata agtcttaata ttcatgcaat
7321 ctaattaaca atcttttctt tgtggttagg actttgagtc ataagaaatt tttctctaca
7381 ctgaagtcat gatggcatgc ttctatatta ttttctaaaa gatttaaagt tttgccttct
7441 ccatttagac ttataattca ctggaatttt tttgtgtgta tggtatgaca tatgggttcc
7501 cttttatttt ttacatataa atatatttcc ctgtttttct aaaaaagaaa aagatcatca
7561 ttttcccatt gtaaaatgcc atattttttt cataggtcac ttacatatat caatgggtct
7621 gtttctgagc tctactctat tttatcagcc tcactgtcta tccccacaca tctcatgctt
7681 tgctctaaat cttgatattt agtggaacat tctttcccat tttgttctac aagaatattt
7741 ttgttattgt cttttgggct tctatataca ttttagaatg aggttggcaa gttaacaaac
7801 agcttttttg gggtgaacat attgactaca aatttatgtg gaaagaaagt accaagttga
7861 ccagtgccgt tccggtgctc accgcgcgcg acgtcgccgg agcggtcgag ttctggaccg
7921 accggctcgg gttctcccgg gacttcgtgg aggacgactt cgccggtgtg gtccgggacg
7981 acgtgaccct gttcatcagc gcggtccagg accaggtggt gccggacaac accctggcct
8041 gggtgtgggt gcgcggcctg gacgagctgt acgccgagtg gtcggaggtc gtgtccacga
8101 acttccggga cgcctccggg ccggccatga ccgagatcgg cgagcagccg tgggggcggg
8161 agttcgccct gcgcgacccg gccggcaact gcgtgcactt cgtggccgag gagcaggact
8221 gacacgtgct acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa
8281 tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct
8341 tcgcccaccc caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
8401 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
8461 tcaatgtatc ttatcatgtc tgtataccgt cgacctctag ctagagcttg gcgtaatcat
8521 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
8581 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
8641 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
8701 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
8761 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
8821 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
8881 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
8941 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
9001 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
9061 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
9121 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
9181 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
9241 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
9301 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
9361 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
9421 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
9481 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
9541 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
9601 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
9661 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
9721 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
9781 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
9841 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
9901 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
9961 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
10021 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
10081 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
10141 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
10201 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
10261 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
10321 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
10381 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
10441 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
10501 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
10561 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
10621 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtcg
10681 acggatcggg agatctcccg atcccctatg gtgcactctc agtacaatct gctctgatgc
10741 cgcatagtta agccagtatc tgctccctgc ttgtgtgttg gaggtcgctg agtagtgcgc
10801 gagcaaaatt taagctacaa caaggcaagg c
//
