| 质粒名称: | p2CT-His-MBP-Lba_Cas13a_WT |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 91867 |
| 存储实验室: | Jennifer Doudna |
|
载体骨架基本信息 |
|
| 载体名称: | p2CT |
| 载体出品公司: | UC Berkeley Macrolab |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | -- |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 细菌表达 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | LbaCas13a (LbaC2c2) WT |
| 物种来源: | -- |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | His6-MBP-TEV site（主干上的N终端） |
| 克隆方法: | Ligation Independent 克隆 |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | p2CT-His-MBP-Lba_Cas13a_WT was a gift from Jennifer Doudna (Addgene plasmid # 91867 ; http://n2t.net/addgene:91867 ; RRID:Addgene_91867) |
| 参考文献部分: | RNA Targeting by Functionally Orthogonal Type VI-A CRISPR-Cas Enzymes. East-Seletsky A, O'Connell MR, Burstein D, Knott GJ, Doudna JA. Mol Cell. 2017 May 4;66(3):373-383.e3. doi: 10.1016/j.molcel.2017.04.008. 10.1016/j.molcel.2017.04.008 PubMed 28475872 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS p2CT-His-MBP-Lba_Cas13a_WT 10267 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION Bacterial expression for Cas13a.
ACCESSION .
VERSION .
KEYWORDS p2CT-His-MBP-Lba_Cas13a_WT
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 10267)
AUTHORS East-Seletsky A, O'Connell MR, Burstein D, Knott GJ, Doudna JA
TITLE RNA Targeting by Functionally Orthogonal Type VI-A CRISPR-Cas
Enzymes.
JOURNAL Mol Cell. 2017 May 4;66(3):373-383.e3. doi:
10.1016/j.molcel.2017.04.008.
PUBMED 28475872
REFERENCE 2 (bases 1 to 10267)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..10267
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter complement(233..261)
/label=tet promoter
/note="E. coli promoter for tetracycline efflux protein
gene"
primer_bind complement(288..306)
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
promoter 374..478
/gene="bla"
/label=AmpR promoter
CDS 479..1339
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(697..716)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 1510..2098
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 1999..2018
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 2252..2269
/label=L4440
/note="L4440 vector, forward primer"
misc_feature 2284..2423
/label=bom
/note="basis of mobility region from pBR322"
primer_bind complement(2349..2368)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 2509..2531
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
CDS complement(2525..2716)
/codon_start=1
/gene="rop"
/product="Rop protein, which maintains plasmids at low copy
number"
/label=rop
/translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
DELYRSCLARFGDDGENL"
primer_bind 4174..4193
/label=pBRrevBam
/note="pBR322 vectors, tet region, downstream of BamHI,
reverse primer"
primer_bind 4277..4296
/label=T7
/note="T7 promoter, forward primer"
promoter 4277..4295
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
RBS 4384..4406
/note="efficient ribosome binding site from bacteriophage
T7 gene 10 (Olins and Rangwala, 1989)"
CDS 4426..4443
/codon_start=1
/product="6xHis affinity tag"
/label=6xHis
/translation="HHHHHH"
CDS 4453..5553
/codon_start=1
/gene="malE (mutated)"
/product="maltose binding protein from E. coli"
/label=MBP
/note="This version of the gene does not encode a signal
sequence, so MBP will remain in the cytosol."
/translation="MKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLE
EKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKL
IAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIA
ADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGE
TAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFL
ENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAF
WYAVRTAVINAASGRQTVDEALKDAQT"
primer_bind 5527..5550
/label=MBP-F
/note="Maltose binding protein, forward primer"
CDS 5608..5628
/codon_start=1
/product="tobacco etch virus (TEV) protease recognition and
cleavage site"
/label=TEV site
/translation="ENLYFQS"
primer_bind complement(10073..10091)
/label=T7 Term
/note="T7 terminator, reverse primer"
terminator 10087..10134
/label=T7 terminator
/note="transcription terminator for bacteriophage T7 RNA
polymerase"
ORIGIN
1 acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg
61 gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag
121 cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat
181 ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc
241 ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg
301 cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt
361 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
421 tccgctcatg agacaataac cctgataaat gcttcaataa cattgaaaaa ggaagagtat
481 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
541 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
601 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
661 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
721 tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
781 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
841 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
901 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
961 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
1021 tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
1081 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
1141 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
1201 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
1261 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
1321 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
1381 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
1441 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
1501 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
1561 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
1621 aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg
1681 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
1741 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
1801 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
1861 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
1921 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
1981 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
2041 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
2101 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
2161 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
2221 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaca
2281 gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcaatggtgc
2341 actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc
2401 tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac
2461 gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca
2521 tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat
2581 cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga
2641 gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt
2701 tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga
2761 taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt
2821 tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa
2881 tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc
2941 agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt
3001 ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg
3061 ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag
3121 taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc
3181 gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg
3241 cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat
3301 tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt
3361 cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc
3421 ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt
3481 gacgatcagc ggtccaatga tcgaagttag gctggtaaga gccgcgagcg atccttgaag
3541 ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc
3601 gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa
3661 cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc
3721 gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc
3781 gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa
3841 aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat
3901 aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc
3961 tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag
4021 tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc
4081 gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat
4141 gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc
4201 aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat
4261 ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctagt
4321 gccggctccg gagagctctt taattaagcg gccgccctgc aggactcgag ttctagaaat
4381 aattttgttt aactttaaga aggagatata catatgaaat cttctcacca tcaccatcac
4441 catggttctt ctatgaaaat cgaagaaggt aaactggtaa tctggattaa cggcgataaa
4501 ggctataacg gtctcgctga agtcggtaag aaattcgaga aagataccgg aattaaagtc
4561 accgttgagc atccggataa actggaagag aaattcccac aggttgcggc aactggcgat
4621 ggccctgaca ttatcttctg ggcacacgac cgctttggtg gctacgctca atctggcctg
4681 ttggctgaaa tcaccccgga caaagcgttc caggacaagc tgtatccgtt tacctgggat
4741 gccgtacgtt acaacggcaa gctgattgct tacccgatcg ctgttgaagc gttatcgctg
4801 atttataaca aagatctgct gccgaacccg ccaaaaacct gggaagagat cccggcgctg
4861 gataaagaac tgaaagcgaa aggtaagagc gcgctgatgt tcaacctgca agaaccgtac
4921 ttcacctggc cgctgattgc tgctgacggg ggttatgcgt tcaagtatga aaacggcaag
4981 tacgacatta aagacgtggg cgtggataac gctggcgcga aagcgggtct gaccttcctg
5041 gttgacctga ttaaaaacaa acacatgaat gcagacaccg attactccat cgcagaagct
5101 gcctttaata aaggcgaaac agcgatgacc atcaacggcc cgtgggcatg gtccaacatc
5161 gacaccagca aagtgaatta tggtgtaacg gtactgccga ccttcaaggg tcaaccatcc
5221 aaaccgttcg ttggcgtgct gagcgcaggt attaacgccg ccagtccgaa caaagagctg
5281 gcaaaagagt tcctcgaaaa ctatctgctg actgatgaag gtctggaagc ggttaataaa
5341 gacaaaccgc tgggtgccgt agcgctgaag tcttacgagg aagagttggc gaaagatcca
5401 cgtattgccg ccactatgga aaacgcccag aaaggtgaaa tcatgccgaa catcccgcag
5461 atgtccgctt tctggtatgc cgtgcgtact gcggtgatca acgccgccag cggtcgtcag
5521 actgtcgatg aagccctgaa agacgcgcag actaattcga gctcgaacaa caacaacaat
5581 aacaataaca acaacctcgg gatcgaggaa aacctgtact tccaatccaa tgcaatgaag
5641 atcagtaaag tacgcgaaga aaaccgtggg gccaaattga ctgtgaacgc caagactgct
5701 gtagtgtccg agaaccgttc gcaagaaggg atcctgtata atgacccaag tcgttacggt
5761 aaaagccgca aaaatgacga ggatcgtgac cgctacatcg agtcccgtct taagtcttct
5821 gggaaattat accgcatttt taatgaggac aagaacaaac gcgagacaga cgaactgcaa
5881 tggttcctta gtgagatcgt gaagaagatc aatcgtcgca atggtttggt attatcggat
5941 atgcttagtg tggacgaccg cgcattcgag aaagccttcg aaaagtatgc agagttatcg
6001 tatacgaatc gccgtaataa agtttctggt agcccggcgt tcgagacctg cggagtagat
6061 gcagcaacag ctgaacgttt aaaaggcatt atctcggaaa ccaactttat caaccgtatc
6121 aaaaacaata tcgacaacaa agtctcggag gacattatcg atcgtattat cgctaagtac
6181 ttgaagaaaa gcttgtgtcg tgagcgtgtc aaacgtgggt taaagaaact tttaatgaat
6241 gccttcgact tgccttacag tgaccctgac atcgatgtgc aacgcgattt tattgattac
6301 gttttagagg atttctacca tgttcgtgcc aagtcacaag tgtctcgttc gatcaaaaac
6361 atgaatatgc cggtgcagcc cgaaggcgat ggtaagtttg cgatcacagt aagtaagggt
6421 gggacggaat cggggaacaa acgctctgcc gaaaaagaag cattcaaaaa gttcctgagt
6481 gattacgcgt cgctggatga acgtgttcgt gacgacatgc ttcgtcgcat gcgccgcctg
6541 gtcgtcttgt acttttacgg atcagacgat agcaaattat cagatgttaa cgaaaaattc
6601 gacgtatggg aggaccatgc tgcccgccgc gtagacaatc gcgagttcat caagcttccg
6661 ttggagaata aacttgccaa cggcaagacc gataaagatg cggaacgtat ccgtaagaac
6721 accgttaaag aactgtatcg taaccaaaat attggatgct atcgccaggc tgttaaagcc
6781 gtagaggagg acaacaacgg tcgctacttc gacgacaaga tgctgaacat gttctttatt
6841 caccgcatcg agtacggtgt ggaaaagatt tacgccaatt tgaaacaagt cacggagttc
6901 aaggcccgca ctgggtatct ttcagagaaa atctggaaag acttgatcaa ttacatttcc
6961 attaaataca tcgctatggg caaggcagtc tacaattacg ccatggatga gctgaatgcc
7021 tcagacaaaa aggagatcga gctgggcaag atcagtgaag aatatttgtc cgggatcagt
7081 tcgtttgact atgaattgat caaagccgaa gagatgctgc aacgcgagac cgctgtatat
7141 gttgctttcg cggctcgcca cttgtcctcc cagaccgttg aactggattc cgagaactcc
7201 gacttccttc ttttgaagcc aaagggaact atggacaaga acgataaaaa caagttagcc
7261 tccaacaaca ttcttaattt tcttaaggac aaggaaacac ttcgcgatac cattttacag
7321 tactttggcg gccacagtct gtggaccgac tttccgtttg acaaatactt ggccggcggg
7381 aaagacgacg tcgacttcct gacagacctt aaagatgtga tttactccat gcgtaatgat
7441 agttttcact atgctactga aaaccacaac aatggaaaat ggaataagga attaattagt
7501 gcaatgtttg aacatgaaac agagcgtatg acagtagtca tgaaagataa attctactcg
7561 aataatcttc ctatgttcta taagaatgac gacttgaaga aattgctgat cgatttgtac
7621 aaggataacg tagagcgtgc gagccaggtc ccatctttta acaaggtctt cgtgcgcaag
7681 aattttcctg ctttggttcg cgataaagac aacttgggga ttgaattaga ccttaaggcg
7741 gacgcggata aaggtgagaa cgagttaaaa ttctacaacg ctctttatta catgtttaaa
7801 gagatctact ataacgcgtt cttaaatgat aagaatgtgc gtgaacgttt tattactaag
7861 gcgacgaaag tggccgataa ctatgatcgc aataaggagc gcaaccttaa agaccgtatt
7921 aagtccgcgg ggtcggatga gaaaaaaaag ttacgtgaac agctgcagaa ttacattgct
7981 gagaatgact tcgggcagcg tatcaagaat atcgtgcagg tcaatccgga ttataccctg
8041 gctcaaatct gccaacttat tatgacggag tataatcaac aaaacaacgg gtgcatgcag
8101 aaaaagtctg ctgcccgcaa agacatcaat aaggatagtt accagcatta caagatgctg
8161 cttttggtta atttacgcaa agcatttttg gaatttatta aggagaatta tgcttttgtt
8221 ttgaaaccct ataaacacga cctttgtgat aaagccgatt tcgtgccaga tttcgccaaa
8281 tacgtcaaac cctacgctgg cttgatcagc cgtgtagcag gatcaagtga gctgcaaaag
8341 tggtacatcg tgagccgctt cttgtctccg gcacaggcca atcacatgct tgggttcctg
8401 cactcgtaca agcagtacgt atgggatatt taccgccgcg catccgaaac cggtacagag
8461 attaaccact ctatcgcaga ggacaagatt gctggggttg acattaccga cgtcgacgca
8521 gtgatcgatt tatcggtgaa attgtgcgga acaatctcta gcgagatttc ggattatttc
8581 aaggatgacg aggtctacgc ggagtacatc tcatcatatt tagatttcga atacgatggt
8641 ggtaattaca aagactcgct gaaccgtttc tgtaattcag acgcagtaaa tgaccaaaaa
8701 gtggcactgt attatgacgg cgaacacccg aaattgaatc gtaacattat cttgtcgaaa
8761 ctttacggag agcgccgttt cttggagaaa atcactgacc gcgtttctcg ttcagatatt
8821 gtggagtatt acaagctgaa gaaggagacg tcgcagtatc agacgaaagg gattttcgat
8881 agtgaggacg aacagaagaa tattaagaag tttcaggaga tgaaaaacat cgtggaattt
8941 cgtgatctga tggattactc ggagatcgcg gacgaattac aaggacaact gatcaactgg
9001 atttatcttc gtgaacgtga cctgatgaac ttccaactgg ggtatcatta cgcctgcttg
9061 aacaacgact caaataagca agctacttat gtaactcttg attaccaagg taaaaaaaac
9121 cgcaagatta acggtgcgat tttatatcag atttgtgcaa tgtatattaa tggactgccg
9181 ctttactacg tcgataaaga cagttcggag tggacggtga gcgacggtaa ggaatccacg
9241 ggagccaaga ttggtgaatt ctaccgctat gcaaagtcct ttgaaaatac gtctgactgc
9301 tacgcttcgg gcttagagat cttcgagaat atcagtgagc acgacaacat cacggaatta
9361 cgcaactaca ttgaacattt tcgttactac agctcttttg accgttcctt tttggggatc
9421 tattcagagg tgtttgatcg ctttttcaca tacgacttaa agtatcgcaa aaacgtacct
9481 accatcttgt ataatatttt actgcaacac tttgttaatg tccgcttcga gttcgtaagc
9541 ggcaaaaaaa tgattggcat tgacaaaaag gaccgcaaga ttgccaaaga gaaggaatgc
9601 gctcgtatta caatccgcga aaagaacggt gtctactcgg agcagttcac ttacaagttg
9661 aagaatggga ccgtgtatgt cgatgcgcgt gacaaacgct acctgcagtc cattattcgt
9721 cttttgtttt accccgaaaa ggtcaacatg gacgaaatga tcgaagttaa ggaaaagaag
9781 aagccgagtg acaataatac gggaaagggc tatagcaaac gcgaccgcca gcaggatcgt
9841 aaagaatatg acaagtacaa agaaaaaaag aaaaaggagg ggaatttcct ttcaggtatg
9901 ggaggcaata tcaactggga cgagattaac gctcaactta agaactagta aattggaagt
9961 ggataacgga tccgcgatcg tggcgcgcca cctggtggcc ggccggtacc acgcgtgcgc
10021 gctgatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag
10081 caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa
10141 ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat
10201 agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc
10261 tccgaga
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