| 质粒名称: | pAC-94N |
|---|---|
| 目录编号: | 53281 |
| 存储实验室: | Francis X Cunningham Jr |
|
载体骨架基本信息 |
|
| 载体名称: | pAC-BETA |
| 载体出品公司: | Francis X. Cunningham, Jr. |
| 完整质粒大小: | 9268 bp |
| 载体抗性: | Chloramphenicol, 25 μg/mL |
| 生长温度: | 30 ℃ |
| 宿主菌: | Top10 |
| 拷贝数: | 低拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | crtY, crtI, crtB |
| 物种来源: | Erwinia herbicola Eho10 |
| 插入大小: | 10609 bp |
| 基因突变: | Plasmid pAC-BETA was digested with EcoRV (to delete the N terminal portion of the crtE gene), and a ca. 9.27 kB fragment was then gel-purified and ligated to give pAC-94N. |
| GenBank编号 | M87280.1 |
| 启动子: | endogenous promoters |
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | EcoRV(未破坏) |
| 3’克隆位点: | EcoRV (未破坏) |
| 5’测序引物 | 无 |
| 3’测序引物 | 无 |
|
文献引用方法 |
|
| 材料和方法部分: | pAC-94N was a gift from Francis X Cunningham Jr (Addgene plasmid # 53281 ; http://n2t.net/addgene:53281 ; RRID:Addgene_53281) |
| 参考文献部分: | A portfolio of plasmids for identification and analysis of carotenoid pathway enzymes: Adonis aestivalis as a case study. Cunningham FX Jr, Gantt E. Photosynth Res. 2007 May;92(2):245-59. Epub 2007 Jul 17. 10.1007/s11120-007-9210-0 PubMed 17634749 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pAC-94N 9268 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Contains crtI, crtY, and crtI genes of Erwinia herbicola (Pantoea
agglomerans) Eho10, and produces beta-carotene in E. coli when
complemented with a gene encoding geranylgeranyl diphosphate
synthase.
ACCESSION .
VERSION .
KEYWORDS pAC-94N
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9268)
AUTHORS Cunningham FX Jr, Gantt E
TITLE A portfolio of plasmids for identification and analysis of
carotenoid pathway enzymes: Adonis aestivalis as a case study.
JOURNAL Photosynth Res. 2007 May;92(2):245-59. Epub 2007 Jul 17.
PUBMED 17634749
REFERENCE 2 (bases 1 to 9268)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..9268
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind 134..153
/label=CAT-R
/note="Chloramphenicol resistance gene, reverse primer"
promoter complement(220..322)
/label=cat promoter
/note="promoter of the E. coli cat gene encoding
chloramphenicol acetyltransferase"
primer_bind complement(714..731)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(848..1393)
/direction=LEFT
/label=p15A ori
/note="Plasmids containing the medium-copy-number p15A
origin of replication can be propagated in E. coli cells
that contain a second plasmid with the ColE1 origin.
"
primer_bind complement(928..947)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
promoter 1505..1533
/label=tet promoter
/note="E. coli promoter for tetracycline efflux protein
gene"
primer_bind complement(6956..6975)
/label=pBRrevBam
/note="pBR322 vectors, tet region, downstream of BamHI,
reverse primer"
CDS complement(8828..219)
/codon_start=1
/gene="cat"
/product="chloramphenicol acetyltransferase"
/label=CmR
/note="confers resistance to chloramphenicol"
/translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
ORIGIN
1 gaattccgga tgagcattca tcaggcgggc aagaatgtga ataaaggccg gataaaactt
61 gtgcttattt ttctttacgg tctttaaaaa ggccgtaata tccagctgaa cggtctggtt
121 ataggtacat tgagcaactg actgaaatgc ctcaaaatgt tctttacgat gccattggga
181 tatatcaacg gtggtatatc cagtgatttt tttctccatt ttagcttcct tagctcctga
241 aaatctcgat aactcaaaaa atacgcccgg tagtgatctt atttcattat ggtgaaagtt
301 ggaacctctt acgtgccgat caacgtctca ttttcgccaa aagttggccc agggcttccc
361 ggtatcaaca gggacaccag gatttattta ttctgcgaag tgatcttccg tcacaggtat
421 ttattcggcg caaagtgcgt cgggtgatgc tgccaactta ctgatttagt gtatgatggt
481 gtttttgagg tgctccagtg gcttctgttt ctatcagctg tccctcctgt tcagctactg
541 acggggtggt gcgtaacggc aaaagcaccg ccggacatca gcgctagcgg agtgtatact
601 ggcttactat gttggcactg atgagggtgt cagtgaagtg cttcatgtgg caggagaaaa
661 aaggctgcac cggtgcgtca gcagaatatg tgatacagga tatattccgc ttcctcgctc
721 actgactcgc tacgctcggt cgttcgactg cggcgagcgg aaatggctta cgaacggggc
781 ggagatttcc tggaagatgc caggaagata cttaacaggg aagtgagagg gccgcggcaa
841 agccgttttt ccataggctc cgcccccctg acaagcatca cgaaatctga cgctcaaatc
901 agtggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggcggctccc
961 tcgtgcgctc tcctgttcct gcctttcggt ttaccggtgt cattccgctg ttatggccgc
1021 gtttgtctca ttccacgcct gacactcagt tccgggtagg cagttcgctc caagctggac
1081 tgtatgcacg aaccccccgt tcagtccgac cgctgcgcct tatccggtaa ctatcgtctt
1141 gagtccaacc cggaaagaca tgcaaaagca ccactggcag cagccactgg taattgattt
1201 agaggagtta gtcttgaagt catgcgccgg ttaaggctaa actgaaagga caagttttgg
1261 tgactgcgct cctccaagcc agttacctcg gttcaaagag ttggtagctc agagaacctt
1321 cgaaaaaccg ccctgcaagg cggttttttc gttttcagag caagagatta cgcgcagacc
1381 aaaacgatct caagaagatc atcttattaa tcagataaaa tatttctaga tttcagtgca
1441 atttatctct tcaaatgtag cacctgaagt cagccccata cgatataagt tgtaattctc
1501 atgtttgaca gcttatcatc gataagcttt aatgcggtag tttatcacag ttaaattgct
1561 aacgcagtca ggcaccgtgt atgaaatcta acaatgcgct catcgtcatc ctcggcaccg
1621 tcaccctgga tgctgtaggc ataggcttgg ttatgccggt actgccgggc ctcttgcggg
1681 atatcgttct cgacccccgt cgcgccgtaa ctcaggctag cgcaggtttt gagcgctggc
1741 gctttaccca ctgcgccctg ccagagctga attttagcga catcacgctg gaaaccacct
1801 tcctgaatcg gcagctacag gctccgctgc tgatcagctc catgaccggc ggcgttgagc
1861 gctcgcgcca tatcaaccgc cacctcgccg aggcggcgca ggtgctaaaa attgcgatgg
1921 gggtgggctc ccagcgcgtc gccattgaga gcgacgcggg cttagggctg gataaaaccc
1981 tgcggcagct ggctccggac gtgccgctgc tggcgaacct cggcgcggcg cagctgaccg
2041 gcagaaaagg tattgattac gcccgacggg ccgtggagat gatcgaggcg gatgcgctga
2101 ttgtgcacct aaacccgctg caggaggcgc tacagcccgg cggcgatcgc gactggcgcg
2161 gacggctggc ggctattgaa actctggtcc gcgagctgcc cgttccgctg gtggtgaaag
2221 aggtgggagc cggtatctcc cgaaccgtgg ccgggcagct gatcgatgcc ggcgttaccg
2281 tgattgacgt cgcgggcgcg ggcggcacca gctgggccgc cgttgaaggc gagcgggcgg
2341 ccaccgagca gcagcgcagc gtggccaacg tctttgccga ctgggggatc cccaccgctg
2401 aggcgctggt tgacattgcc gaggcctggc cgcagatgcc ccttattgcc tcgggcggga
2461 ttaaaaacgg cgtcgaccag ccgcgctacg ttgccgaggc taatctggtg atcacccacg
2521 gcggtctcaa taccgtactg gatgcgctgg ctgccgcgac gccggtgctg gcggtgccac
2581 tctctttcga ccagcccgcc gtggctgccc ggctggtcta taacgggctg ggtcgccggg
2641 tatcgcgctt tgccagacag cagacgctgg cggatgagat tgcccaactg ctgggggatg
2701 agacgctgca tcagcgtctg gcgacggccc gccagcagct taacgacgcc gggggcacgc
2761 cccgtgcggc gaccctgatt gaacaggcca tagcagggag tgagagcgta tcgtgaggga
2821 tctgatttta gtcggcggcg gcctggccaa cgggctgatc gcctggcgtc tgcgccagcg
2881 ctacccgcag cttaacctgc tgctgatcga ggccggggag cagcccggcg ggaaccatac
2941 ctggtcattc catgaagacg atctgactcc cgggcagcac gcctggctgg ccccgctggt
3001 ggcccacgcc tggccgggct atgaggtgca gtttcccgat cttcgccgtc gcctcgcgcg
3061 cggctactac tccattacct cagagcgctt tgccgaggcc ctgcatcagg cgctggggga
3121 gaacatctgg ctaaactgtt cggtgagcga ggtgttaccc aatagcgtgc gccttgccaa
3181 cggtgaggcg ctgcttgccg gagcggtgat tgacggacgc ggcgtgaccg ccagttcggc
3241 gatgcaaacc ggctatcagc tctttcttgg tcagcagtgg cggctgacac agccccacgg
3301 cctgaccgta ccgatcctga tggatgccac ggtggcgcag cagcagggct atcgctttgt
3361 ctacacgctg ccgctctccg ccgacacgct gctgatcgag gatacgcgct acgccaatgt
3421 cccgcagcgt gatgataatg ccctacgcca gacggttacc gactatgctc acagcaaagg
3481 gtggcagctg gcccagcttg aacgcgagga gaccggctgt ctgccgatta ccctggcggg
3541 tgacatccag gctctgtggg ccgatgcgcc gggcgtgccg cgctcgggaa tgcgggctgg
3601 gctatttcac cctaccactg gctattcgct gccgctggcg gtggcccttg ccgacgcgat
3661 tgccgacagc ccgcggctgg gcagcgttcc gctctatcag ctcacccggc agtttgccga
3721 acgccactgg cgcaggcagg gattcttccg cctgctgaac cggatgcttt tcctggccgg
3781 gcgcgaggag aaccgctggc gggtgatgca gcgcttttat gggctgccgg agcccaccgt
3841 agagcgcttt tacgccggtc ggctctctct ctttgataag gcccgcattt tgacgggcaa
3901 gccaccggtt ccgctgggcg aagcctggcg ggcggcgctg aaccattttc ctgacagacg
3961 agataaagga tgaaaaaaac cgttgtgatt ggcgcaggct ttggtggcct ggcgctggcg
4021 attcgcctgc aggcggcagg gatcccaacc gtactgctgg agcagcggga caagcccggc
4081 ggtcgggcct acgtctggca tgaccagggc tttacctttg acgccgggcc gacggtgatc
4141 accgatccta ccgcgcttga ggcgctgttc accctggccg gcaggcgcat ggaggattac
4201 gtcaggctgc tgccggtaaa acccttctac cgactctgct gggagtccgg gaagaccctc
4261 gactatgcta acgacagcgc cgagcttgag gcgcagatta cccagttcaa cccccgcgac
4321 gtcgagggct accggcgctt tctggcttac tcccaggcgg tattccagga gggatatttg
4381 cgcctcggca gcgtgccgtt cctctctttt cgcgacatgc tgcgcgccgg gccgcagctg
4441 cttaagctcc aggcgtggca gagcgtctac cagtcggttt cgcgctttat tgaggatgag
4501 catctgcggc aggccttctc gttccactcc ctgctggtag gcggcaaccc cttcaccacc
4561 tcgtccatct acaccctgat ccacgccctt gagcgggagt ggggggtctg gttccctgag
4621 ggcggcaccg gggcgctggt gaacggcatg gtgaagctgt ttaccgatct gggcggggag
4681 atcgaactca acgcccgggt cgaagagctg gtggtggccg ataaccgcgt aagccaggtc
4741 cggctggcgg atggtcggat ctttgacacc gacgccgtag cctcgaacgc tgacgtggtg
4801 aacacctata aaaagctgct cggccaccat ccggtggggc agaagcgggc ggcagcgctg
4861 gagcgcaaga gcatgagcaa ctcgctgttt gtgctctact tcggcctgaa ccagcctcat
4921 tcccagctgg cgcaccatac catctgtttt ggtccccgct accgggagct gatcgacgag
4981 atctttaccg gcagcgcgct ggcggatgac ttctcgctct acctgcactc gccctgcgtg
5041 accgatccct cgctcgcgcc tcccggctgc gccagcttct acgtgctggc cccggtgccg
5101 catcttggca acgcgccgct ggactgggcg caggaggggc cgaagctgcg cgaccgcatc
5161 tttgactacc ttgaagagcg ctatatgccc ggcctgcgta gccagctggt gacccagcgg
5221 atctttaccc cggcagactt ccacgacacg ctggatgcgc atctgggatc ggccttctcc
5281 atcgagccgc tgctgaccca aagcgcctgg ttccgcccgc acaaccgcga cagcgacatt
5341 gccaacctct acctggtggg cgcaggtact caccctgggg cgggcattcc tggcgtagtg
5401 gcctcggcga aagccaccgc cagcctgatg attgaggatc tgcaatgagc caaccgccgc
5461 tgcttgacca cgccacgcag accatggcca acggctcgaa aagttttgcc accgctgcga
5521 agctgttcga cccggccacc cgccgtagcg tgctgatgct ctacacctgg tgccgccact
5581 gcgatgacgt cattgacgac cagacccacg gcttcgccag cgaggccgcg gcggaggagg
5641 aggccaccca gcgcctggcc cggctgcgca cgctgaccct ggcggcgttt gaaggggccg
5701 agatgcagga tccggccttc gctgcctttc aggaggtggc gctgacccac ggtattacgc
5761 cccgcatggc gctcgatcac ctcgacggct ttgcgatgga cgtggctcag acccgctatg
5821 tcacctttga ggatacgctg cgctactgct atcacgtggc gggcgtggtg ggtctgatga
5881 tggccagggt gatgggcgtg cgggatgagc gggtgctgga tcgcgcctgc gatctggggc
5941 tggccttcca gctgacgaat atcgcccggg atattattga cgatgcggct attgaccgct
6001 gctatctgcc cgccgagtgg ctgcaggatg ccgggctgac cccggagaac tatgccgcgc
6061 gggagaatcg ggccgcgctg gcgcgggtgg cggagcggct tattgatgcc gcagagccgt
6121 actacatctc ctcccaggcc gggctacacg atctgccgcc gcgctgcgcc tgggcgatcg
6181 ccaccgcccg cagcgtctac cgggagatcg gtattaaggt aaaagcggcg ggaggcagcg
6241 cctgggatcg ccgccagcac accagcaaag gtgaaaaaat tgccatgctg atggcggcac
6301 cggggcaggt tattcgggcg aagacgacga gggtgacgcc gcgtccggcc ggtctttggc
6361 agcgtcccgt ttaggcgggc ggccatgacg ttcacgcagg atcgcctgta ggtcggcagg
6421 cttgcgggcg taaataaaac cgaaggagac gcagccctcc cggccgcgca ccgcgtggtg
6481 caggcggtgg gcgacgtaga gccgcttcag gtagccccgg cgcgggatcc cgcctcgccg
6541 tgctgtccgg tctcaacctg atccgccaga atcgagccaa cgggatcggc cagcacgaat
6601 tcggtatgcg gagagtgttt ggcaaaccat gcctgcaagc cacccagcgt gccgccggag
6661 ccaacgccta ctaccacggc atcaaccctg cctgccagct ggtcgaacag ctccggtgcg
6721 gtggtggtgg cgtgcgccag cgggttggcc gggttagaga actgatcaat atagtaagca
6781 cccggcgtct cctctgccag gcggcgggcg tagtcctgat agtactccgg gtggcccttt
6841 gtcacgtcgg agcgggtcag gcgcacatca acacccagcg cacgcaggtg gtagatcctc
6901 tacgccggac gcatcgtggc cggcatcacc ggcgccacag gtgcggttgc tggcgcctat
6961 atcgccgaca tcaccgatgg ggaagatcgg gctcgccact tcgggctcat gagcgcttgt
7021 ttcggcgtgg gtatggtggc aggccccgtg gccgggggac tgttgggcgc catctccttg
7081 catgcaccat tccttgcggc ggcggtgctc aacggcctca acctactact gggctgcttc
7141 ctaatgcagg agtcgcataa gggagagcgt cgaccgatgc ccttgagagc cttcaaccca
7201 gtcagctcct tccggtgggc gcggggcatg actatcgtcg ccgcacttat gactgtcttc
7261 tttatcatgc aactcgtagg acaggtgccg gcagcgctct gggtcatttt cggcgaggac
7321 cgctttcgct ggagcgcgac gatgatcggc ctgtcgcttg cggtattcgg aatcttgcac
7381 gccctcgctc aagccttcgt cactggtccc gccaccaaac gtttcggcga gaagcaggcc
7441 attatcgccg gcatggcggc cgacgcgctg ggctacgtct tgctggcgtt cgcgacgcga
7501 ggctggatgg ccttccccat tatgattctt ctcgcttccg gcggcatcgg gatgcccgcg
7561 ttgcaggcca tgctgtccag gcaggtagat gacgaccatc agggacagct tcaaggatcg
7621 ctcgcggctc ttaccagcct aacttcgatc actggaccgc tgatcgtcac ggcgatttat
7681 gccgcctcgg cgagcacatg gaacgggttg gcatggattg taggcgccgc cctatacctt
7741 gtctgcctcc ccgcgttgcg tcgcggtgca tggagccggg ccacctcgac ctgaatggaa
7801 gccggcggca cctcgctaac ggattcacca ctccaagaat tggagccaat caattcttgc
7861 ggagaactgt gaatgcgcaa accaaccctt ggcagaacat atccatcgcg tccgccatct
7921 ccagcagccg cacgcggcgc atctcgggca gcgttgggtc ctggccacgg gtgcgcatga
7981 tcgtgctcct gtcgttgagg acccggctag gctggcgggg ttgccttact ggttagcaga
8041 atgaatcacc gatacgcgag cgaacgtgaa gcgactgctg ctgcaaaacg tctgcgacct
8101 gagcaacaac atgaatggtc ttcggtttcc gtgtttcgta aagtctggaa acgcggaagt
8161 cccctacgtg ctgctgaagt tgcccgcaac agagagtgga accaaccggt gataccacga
8221 tactatgact gagagtcaac gccatgagcg gcctcatttc ttattctgag ttacaacagt
8281 ccgcaccgct gtccggtagc tccttccggt gggcgcgggg catgactatc gtcgccgcac
8341 ttatgactgt cttctttatc atgcaactcg taggacaggt gccggcagcg cccaacagtc
8401 ccccggccac ggggcctgcc accataccca cgccgaaaca agcgccctgc accattatgt
8461 tccggatctg catcgcagga tgctgctggc taccctgtgg aacacctaca tctgtattaa
8521 cgaagcgcta accgttttta tcaggctctg ggaggcagaa taaatgatca tatcgtcaat
8581 tattacctcc acggggagag cctgagcaaa ctggcctcag gcatttgaga agcacacggt
8641 cacactgctt ccggtagtca ataaaccggt aaaccagcaa tagacataag cggctattta
8701 acgaccctgc cctgaaccga cgaccgggtc gaatttgctt tcgaatttct gccattcatc
8761 cgcttattat cacttattca ggcgtagcac caggcgttta agggcaccaa taactgcctt
8821 aaaaaaatta cgccccgccc tgccactcat cgcagtactg ttgtaattca ttaagcattc
8881 tgccgacatg gaagccatca cagacggcat gatgaacctg aatcgccagc ggcatcagca
8941 ccttgtcgcc ttgcgtataa tatttgccca tggtgaaaac gggggcgaag aagttgtcca
9001 tattggccac gtttaaatca aaactggtga aactcaccca gggattggct gagacgaaaa
9061 acatattctc aataaaccct ttagggaaat aggccaggtt ttcaccgtaa cacgccacat
9121 cttgcgaata tatgtgtaga aactgccgga aatcgtcgtg gtattcactc cagagcgatg
9181 aaaacgtttc agtttgctca tggaaaacgg tgtaacaagg gtgaacacta tcccatatca
9241 ccagctcacc gtctttcatt gccatacg
//
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