| 质粒名称: | pAC-BETAipi |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 53277 |
| 存储实验室: | Francis X Cunningham Jr |
|
载体骨架基本信息 |
|
| 载体名称: | pAC-BETA |
| 载体出品公司: | Francis X. Cunningham, Jr. |
| 载体抗性: | 氯霉素 |
| 空载体大小: | 10609 bp |
| 完整质粒大小: | 9259 bp |
| 载体修饰: | 从pAC BETA中删除1.6kb的SphI-DrdI片段，得到pAC-BETA9.0（未发布）。从pAC-EHER中分离出一个2.6kb的HindIII钝性SphI片段，该片段含有编码2型异戊烯基二磷酸异构酶的CrtE和idi（ORF6），插入pAC-BETA9.0的HindIII-Klenow钝化SalI位点，得到pAC-BETAipi。 |
| 载体类型: | 低拷贝数细菌克隆载体 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 低拷贝 |
| 生长条件: | 30 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | idi,isopentenyl diphosphate isomerase,ORF6 |
| 物种来源: | Erwinia herbicola Eho10 |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | M87280.1 |
| 启动子 | endogenous promoter |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | HindIII(未破坏) |
| 3' 克隆位点: | SalI(未破坏) |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pAC-BETAipi was a gift from Francis X Cunningham Jr (Addgene plasmid # 53277 ; http://n2t.net/addgene:53277 ; RRID:Addgene_53277) |
| 参考文献部分: | A study in scarlet: enzymes of ketocarotenoid biosynthesis in the flowers of Adonis aestivalis. Cunningham FX Jr, Gantt E. Plant J. 2005 Feb;41(3):478-92. 10.1111/j.1365-313X.2004.02309.x PubMed 15659105 |
买家导航
pACBETAipi载体基本信息
pACBETAipi载体图谱质粒图谱和多克隆位点信息
pACBETAipi载体简介
pACBETAipi载体序列
LOCUS pAC-BETAipi 9259 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION Contains ctrE, crtB, crtI, crtY, and idi genes of Erwinia herbicola
(Pantoea agglomerans) Eho10 and thereby produces beta-carotene in
Escherichia coli.
ACCESSION .
VERSION .
KEYWORDS pAC-BETAipi
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9259)
AUTHORS Cunningham FX Jr, Gantt E
TITLE A study in scarlet: enzymes of ketocarotenoid biosynthesis in the
flowers of Adonis aestivalis.
JOURNAL Plant J. 2005 Feb;41(3):478-92.
PUBMED 15659105
REFERENCE 2 (bases 1 to 9259)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..9259
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind 134..153
/label=CAT-R
/note="Chloramphenicol resistance gene, reverse primer"
promoter complement(220..322)
/label=cat promoter
/note="promoter of the E. coli cat gene encoding
chloramphenicol acetyltransferase"
primer_bind complement(714..731)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(848..1393)
/direction=LEFT
/label=p15A ori
/note="Plasmids containing the medium-copy-number p15A
origin of replication can be propagated in E. coli cells
that contain a second plasmid with the ColE1 origin.
"
primer_bind complement(928..947)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
promoter 1505..1533
/label=tet promoter
/note="E. coli promoter for tetracycline efflux protein
gene"
primer_bind complement(8592..8611)
/label=pBRrevBam
/note="pBR322 vectors, tet region, downstream of BamHI,
reverse primer"
CDS complement(8819..219)
/codon_start=1
/gene="cat"
/product="chloramphenicol acetyltransferase"
/label=CmR
/note="confers resistance to chloramphenicol"
/translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
ORIGIN
1 gaattccgga tgagcattca tcaggcgggc aagaatgtga ataaaggccg gataaaactt
61 gtgcttattt ttctttacgg tctttaaaaa ggccgtaata tccagctgaa cggtctggtt
121 ataggtacat tgagcaactg actgaaatgc ctcaaaatgt tctttacgat gccattggga
181 tatatcaacg gtggtatatc cagtgatttt tttctccatt ttagcttcct tagctcctga
241 aaatctcgat aactcaaaaa atacgcccgg tagtgatctt atttcattat ggtgaaagtt
301 ggaacctctt acgtgccgat caacgtctca ttttcgccaa aagttggccc agggcttccc
361 ggtatcaaca gggacaccag gatttattta ttctgcgaag tgatcttccg tcacaggtat
421 ttattcggcg caaagtgcgt cgggtgatgc tgccaactta ctgatttagt gtatgatggt
481 gtttttgagg tgctccagtg gcttctgttt ctatcagctg tccctcctgt tcagctactg
541 acggggtggt gcgtaacggc aaaagcaccg ccggacatca gcgctagcgg agtgtatact
601 ggcttactat gttggcactg atgagggtgt cagtgaagtg cttcatgtgg caggagaaaa
661 aaggctgcac cggtgcgtca gcagaatatg tgatacagga tatattccgc ttcctcgctc
721 actgactcgc tacgctcggt cgttcgactg cggcgagcgg aaatggctta cgaacggggc
781 ggagatttcc tggaagatgc caggaagata cttaacaggg aagtgagagg gccgcggcaa
841 agccgttttt ccataggctc cgcccccctg acaagcatca cgaaatctga cgctcaaatc
901 agtggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggcggctccc
961 tcgtgcgctc tcctgttcct gcctttcggt ttaccggtgt cattccgctg ttatggccgc
1021 gtttgtctca ttccacgcct gacactcagt tccgggtagg cagttcgctc caagctggac
1081 tgtatgcacg aaccccccgt tcagtccgac cgctgcgcct tatccggtaa ctatcgtctt
1141 gagtccaacc cggaaagaca tgcaaaagca ccactggcag cagccactgg taattgattt
1201 agaggagtta gtcttgaagt catgcgccgg ttaaggctaa actgaaagga caagttttgg
1261 tgactgcgct cctccaagcc agttacctcg gttcaaagag ttggtagctc agagaacctt
1321 cgaaaaaccg ccctgcaagg cggttttttc gttttcagag caagagatta cgcgcagacc
1381 aaaacgatct caagaagatc atcttattaa tcagataaaa tatttctaga tttcagtgca
1441 atttatctct tcaaatgtag cacctgaagt cagccccata cgatataagt tgtaattctc
1501 atgtttgaca gcttatcatc gataagcttt aatgcggtag tttatcacag ttaaattgct
1561 aacgcagtca ggcaccgtgt atgaaatcta acaatgcgct catcgtcatc ctcggcaccg
1621 tcaccctgga tgctgtaggc ataggcttgg ttatgccggt actgccgggc ctcttgcggg
1681 atatcgtcca ttccgacagc atcgccagtc actatggcgt gctgctagcg ctatatgcgt
1741 tgatgcaatt tctatgcgca cccgttctcg gagcactgtc cgaccgcttt ggccgccgcc
1801 cagtcctgct cgcttcgcta cttggagcca ctatcgacta cgcgatcatg gcgaccacac
1861 ccgtcctgtg gatctaaagg cacagcgtct catgcttcgc acaatgtaaa actgcttcag
1921 aacctggcga gagctatccg cgcggtctac ggttaactga tactaaaaga caattcagcg
1981 ggtaaccttg caatggtgag tggcagtaaa gcgggcgttt cgcctcatcg cgaaatagaa
2041 gtaatgagac aatccattga cgatcacctg gctggcctgt tacctgaaac cgacagccag
2101 gatatcgtca gccttgcgat gcgtgaaggc gtcatggcac ccggtaaacg gatccgtccg
2161 ctgctgatgc tgctggccgc ccgcgacctc cgctaccagg gcagtatgcc tacgctgctc
2221 gatctcgcct gcgccgttga actgacccat accgcgtcgc tgatgctcga cgacatgccc
2281 tgcatggaca acgccgagct gcgccgcggt cagcccacta cccacaaaaa atttggtgag
2341 agcgtggcga tccttgcctc cgttgggctg ctctctaaag cctttggtct gatcgccgcc
2401 accggcgatc tgccggggga gaggcgtgcc caggcggtca acgagctctc taccgccgtg
2461 ggcgtgcagg gcctggtact ggggcagttt cgcgatctta acgatgccgc cctcgaccgt
2521 acccctgacg ctatcctcag caccaaccac ctcaagaccg gcattctgtt cagcgcgatg
2581 ctgcagatcg tcgccattgc ttccgcctcg tcgccgagca cgcgagagac gctgcacgcc
2641 ttcgccctcg acttcggcca ggcgtttcaa ctgctggacg atctgcgtga cgatcacccg
2701 gaaaccggta aagatcgcaa taaggacgcg ggaaaatcga cgctggtcaa ccggctgggc
2761 gcagacgcgg cccggcaaaa gctgcgcgag catattgatt ccgccgacaa acacctcact
2821 tttgcctgtc cgcagggcgg cgccatccga cagtttatgc atctgtggtt tggccatcac
2881 cttgccgact ggtcaccggt catgaaaatc gcctgatacc gcccttttgg gttcaagcag
2941 tacataacga tggaaccaca ttacaggagt agtgatgaat gaaggacgag cgccttgttc
3001 agcgtaagaa cgatcatctg gatatcgttc tcgacccccg tcgcgccgta actcaggcta
3061 gcgcaggttt tgagcgctgg cgctttaccc actgcgccct gccagagctg aattttagcg
3121 acatcacgct ggaaaccacc ttcctgaatc ggcagctaca ggctccgctg ctgatcagct
3181 ccatgaccgg cggcgttgag cgctcgcgcc atatcaaccg ccacctcgcc gaggcggcgc
3241 aggtgctaaa aattgcgatg ggggtgggct cccagcgcgt cgccattgag agcgacgcgg
3301 gcttagggct ggataaaacc ctgcggcagc tggctccgga cgtgccgctg ctggcgaacc
3361 tcggcgcggc gcagctgacc ggcagaaaag gtattgatta cgcccgacgg gccgtggaga
3421 tgatcgaggc ggatgcgctg attgtgcacc taaacccgct gcaggaggcg ctacagcccg
3481 gcggcgatcg cgactggcgc ggacggctgg cggctattga aactctggtc cgcgagctgc
3541 ccgttccgct ggtggtgaaa gaggtgggag ccggtatctc ccgaaccgtg gccgggcagc
3601 tgatcgatgc cggcgttacc gtgattgacg tcgcgggcgc gggcggcacc agctgggccg
3661 ccgttgaagg cgagcgggcg gccaccgagc agcagcgcag cgtggccaac gtctttgccg
3721 actgggggat ccccaccgct gaggcgctgg ttgacattgc cgaggcctgg ccgcagatgc
3781 cccttattgc ctcgggcggg attaaaaacg gcgtcgacgc ggcgaaagcg ctgcggctcg
3841 gcgcgtgcat ggtagggcag gccgccgccg tgctcggcag cgcaggcgtc tccacggaga
3901 aggtgatcga tcacttcaac gtgattattg agcagctgcg ggtggcctgc ttctgcaccg
3961 gcagccgcag cctgagcgat ctaaagcagg ctgatatccg ctatgtgcgg gatacgccat
4021 gagccatttt gccattgtgg caccgccgct ctacagtcat gcggtggcgc tgcatgccct
4081 ggcgctggag atggcccaac gcggccactc gaccagccgc gctacgttgc cgaggctaat
4141 ctggtgatca cccacggcgg tctcaatacc gtactggatg cgctggctgc cgcgacgccg
4201 gtgctggcgg tgccactctc tttcgaccag cccgccgtgg ctgcccggct ggtctataac
4261 gggctgggtc gccgggtatc gcgctttgcc agacagcaga cgctggcgga tgagattgcc
4321 caactgctgg gggatgagac gctgcatcag cgtctggcga cggcccgcca gcagcttaac
4381 gacgccgggg gcacgccccg tgcggcgacc ctgattgaac aggccatagc agggagtgag
4441 agcgtatcgt gagggatctg attttagtcg gcggcggcct ggccaacggg ctgatcgcct
4501 ggcgtctgcg ccagcgctac ccgcagctta acctgctgct gatcgaggcc ggggagcagc
4561 ccggcgggaa ccatacctgg tcattccatg aagacgatct gactcccggg cagcacgcct
4621 ggctggcccc gctggtggcc cacgcctggc cgggctatga ggtgcagttt cccgatcttc
4681 gccgtcgcct cgcgcgcggc tactactcca ttacctcaga gcgctttgcc gaggccctgc
4741 atcaggcgct gggggagaac atctggctaa actgttcggt gagcgaggtg ttacccaata
4801 gcgtgcgcct tgccaacggt gaggcgctgc ttgccggagc ggtgattgac ggacgcggcg
4861 tgaccgccag ttcggcgatg caaaccggct atcagctctt tcttggtcag cagtggcggc
4921 tgacacagcc ccacggcctg accgtaccga tcctgatgga tgccacggtg gcgcagcagc
4981 agggctatcg ctttgtctac acgctgccgc tctccgccga cacgctgctg atcgaggata
5041 cgcgctacgc caatgtcccg cagcgtgatg ataatgccct acgccagacg gttaccgact
5101 atgctcacag caaagggtgg cagctggccc agcttgaacg cgaggagacc ggctgtctgc
5161 cgattaccct ggcgggtgac atccaggctc tgtgggccga tgcgccgggc gtgccgcgct
5221 cgggaatgcg ggctgggcta tttcacccta ccactggcta ttcgctgccg ctggcggtgg
5281 cccttgccga cgcgattgcc gacagcccgc ggctgggcag cgttccgctc tatcagctca
5341 cccggcagtt tgccgaacgc cactggcgca ggcagggatt cttccgcctg ctgaaccgga
5401 tgcttttcct ggccgggcgc gaggagaacc gctggcgggt gatgcagcgc ttttatgggc
5461 tgccggagcc caccgtagag cgcttttacg ccggtcggct ctctctcttt gataaggccc
5521 gcattttgac gggcaagcca ccggttccgc tgggcgaagc ctggcgggcg gcgctgaacc
5581 attttcctga cagacgagat aaaggatgaa aaaaaccgtt gtgattggcg caggctttgg
5641 tggcctggcg ctggcgattc gcctgcaggc ggcagggatc ccaaccgtac tgctggagca
5701 gcgggacaag cccggcggtc gggcctacgt ctggcatgac cagggcttta cctttgacgc
5761 cgggccgacg gtgatcaccg atcctaccgc gcttgaggcg ctgttcaccc tggccggcag
5821 gcgcatggag gattacgtca ggctgctgcc ggtaaaaccc ttctaccgac tctgctggga
5881 gtccgggaag accctcgact atgctaacga cagcgccgag cttgaggcgc agattaccca
5941 gttcaacccc cgcgacgtcg agggctaccg gcgctttctg gcttactccc aggcggtatt
6001 ccaggaggga tatttgcgcc tcggcagcgt gccgttcctc tcttttcgcg acatgctgcg
6061 cgccgggccg cagctgctta agctccaggc gtggcagagc gtctaccagt cggtttcgcg
6121 ctttattgag gatgagcatc tgcggcaggc cttctcgttc cactccctgc tggtaggcgg
6181 caaccccttc accacctcgt ccatctacac cctgatccac gcccttgagc gggagtgggg
6241 ggtctggttc cctgagggcg gcaccggggc gctggtgaac ggcatggtga agctgtttac
6301 cgatctgggc ggggagatcg aactcaacgc ccgggtcgaa gagctggtgg tggccgataa
6361 ccgcgtaagc caggtccggc tggcggatgg tcggatcttt gacaccgacg ccgtagcctc
6421 gaacgctgac gtggtgaaca cctataaaaa gctgctcggc caccatccgg tggggcagaa
6481 gcgggcggca gcgctggagc gcaagagcat gagcaactcg ctgtttgtgc tctacttcgg
6541 cctgaaccag cctcattccc agctggcgca ccataccatc tgttttggtc cccgctaccg
6601 ggagctgatc gacgagatct ttaccggcag cgcgctggcg gatgacttct cgctctacct
6661 gcactcgccc tgcgtgaccg atccctcgct cgcgcctccc ggctgcgcca gcttctacgt
6721 gctggccccg gtgccgcatc ttggcaacgc gccgctggac tgggcgcagg aggggccgaa
6781 gctgcgcgac cgcatctttg actaccttga agagcgctat atgcccggcc tgcgtagcca
6841 gctggtgacc cagcggatct ttaccccggc agacttccac gacacgctgg atgcgcatct
6901 gggatcggcc ttctccatcg agccgctgct gacccaaagc gcctggttcc gcccgcacaa
6961 ccgcgacagc gacattgcca acctctacct ggtgggcgca ggtactcacc ctggggcggg
7021 cattcctggc gtagtggcct cggcgaaagc caccgccagc ctgatgattg aggatctgca
7081 atgagccaac cgccgctgct tgaccacgcc acgcagacca tggccaacgg ctcgaaaagt
7141 tttgccaccg ctgcgaagct gttcgacccg gccacccgcc gtagcgtgct gatgctctac
7201 acctggtgcc gccactgcga tgacgtcatt gacgaccaga cccacggctt cgccagcgag
7261 gccgcggcgg aggaggaggc cacccagcgc ctggcccggc tgcgcacgct gaccctggcg
7321 gcgtttgaag gggccgagat gcaggatccg gccttcgctg cctttcagga ggtggcgctg
7381 acccacggta ttacgccccg catggcgctc gatcacctcg acggctttgc gatggacgtg
7441 gctcagaccc gctatgtcac ctttgaggat acgctgcgct actgctatca cgtggcgggc
7501 gtggtgggtc tgatgatggc cagggtgatg ggcgtgcggg atgagcgggt gctggatcgc
7561 gcctgcgatc tggggctggc cttccagctg acgaatatcg cccgggatat tattgacgat
7621 gcggctattg accgctgcta tctgcccgcc gagtggctgc aggatgccgg gctgaccccg
7681 gagaactatg ccgcgcggga gaatcgggcc gcgctggcgc gggtggcgga gcggcttatt
7741 gatgccgcag agccgtacta catctcctcc caggccgggc tacacgatct gccgccgcgc
7801 tgcgcctggg cgatcgccac cgcccgcagc gtctaccggg agatcggtat taaggtaaaa
7861 gcggcgggag gcagcgcctg ggatcgccgc cagcacacca gcaaaggtga aaaaattgcc
7921 atgctgatgg cggcaccggg gcaggttatt cgggcgaaga cgacgagggt gacgccgcgt
7981 ccggccggtc tttggcagcg tcccgtttag gcgggcggcc atgacgttca cgcaggatcg
8041 cctgtaggtc ggcaggcttg cgggcgtaaa taaaaccgaa ggagacgcag ccctcccggc
8101 cgcgcaccgc gtggtgcagg cggtgggcga cgtagagccg cttcaggtag ccccggcgcg
8161 ggatcccgcc tcgccgtgct gtccggtctc aacctgatcc gccagaatcg agccaacggg
8221 atcggccagc acgaattcgg tatgcggaga gtgtttggca aaccatgcct gcaagccacc
8281 cagcgtgccg ccggagccaa cgcctactac cacggcatca accctgcctg ccagctggtc
8341 gaacagctcc ggtgcggtgg tggtggcgtg cgccagcggg ttggccgggt tagagaactg
8401 atcaatatag taagcacccg gcgtctcctc tgccaggcgg cgggcgtagt cctgatagta
8461 ctccgggtgg ccctttgtca cgtcggagcg ggtcaggcgc acatcaacac ccagcgcacg
8521 caggtggtag atcctctacg ccggacgcat cgtggccggc atcaccggcg ccacaggtgc
8581 ggttgctggc gcctatatcg ccgacatcac cgatggggaa gatcgggctc gccacttcgg
8641 gctcatgagc gcttgtttcg gcgtgggtat ggtggcaggc cccgtggccg ggggactgtt
8701 gggcgccatc tccttggggt cgaatttgct ttcgaatttc tgccattcat ccgcttatta
8761 tcacttattc aggcgtagca ccaggcgttt aagggcacca ataactgcct taaaaaaatt
8821 acgccccgcc ctgccactca tcgcagtact gttgtaattc attaagcatt ctgccgacat
8881 ggaagccatc acagacggca tgatgaacct gaatcgccag cggcatcagc accttgtcgc
8941 cttgcgtata atatttgccc atggtgaaaa cgggggcgaa gaagttgtcc atattggcca
9001 cgtttaaatc aaaactggtg aaactcaccc agggattggc tgagacgaaa aacatattct
9061 caataaaccc tttagggaaa taggccaggt tttcaccgta acacgccaca tcttgcgaat
9121 atatgtgtag aaactgccgg aaatcgtcgt ggtattcact ccagagcgat gaaaacgttt
9181 cagtttgctc atggaaaacg gtgtaacaag ggtgaacact atcccatatc accagctcac
9241 cgtctttcat tgccatacg
//
