pAWP78-PVCpnf_pvc13-HA

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相关技术服务:质粒构建    基因合成    质粒大提

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载体基本信息

质粒名称: pAWP78-PVCpnf_pvc13-HA
目录编号: 198296
存储实验室: Feng Zhang

载体骨架基本信息
载体名称: pAWP78
完整质粒大小: 25157 bp
载体类型: Bacterial Expression
载体抗性: Kanamycin, 50 μg/mL
生长温度: 37 ℃
宿主菌: DH5a
拷贝数: 低拷贝

插入基因信息
插入基因名称: PVCpnf_pvc13-HA
物种来源: Photorhabdus asymbiotica
克隆方法: Gibson Cloning

文献引用方法
材料和方法部分: pAWP78-PVCpnf_pvc13-HA was a gift from Feng Zhang (Addgene plasmid # 198296 ; http://n2t.net/addgene:198296 ; RRID:Addgene_198296)
参考文献部分: Programmable protein delivery with a bacterial contractile injection system. Kreitz J, Friedrich MJ, Guru A, Lash B, Saito M, Macrae RK, Zhang F. Nature. 2023 Apr;616(7956):357-364. doi: 10.1038/s41586-023-05870-7. Epub 2023 Mar 29. 10.1038/s41586-023-05870-7 PubMed 36991127

载体图谱质粒图谱和多克隆位点信息

pAWP78-PVCpnf_pvc13-HA

载体简介

载体序列

LOCUS       pAWP78-PVCpnf_pvc13-HA               25157 bp ds-DNA     circular SYN 27-MAR-2023
DEFINITION  Produces PVCs retargeted with HA tag.
ACCESSION   .
VERSION     .
KEYWORDS    pAWP78-PVCpnf_pvc13-HA
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 25157)
  AUTHORS   Kreitz J, Friedrich MJ, Guru A, Lash B, Saito M, Macrae RK, Zhang F
  TITLE     Programmable protein delivery with a bacterial contractile injection
            system
  JOURNAL   Nature March 29, 2023
REFERENCE   2  (bases 1 to 25157)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Mar 27, 2023 from SnapGene Server 1.1.58
            http://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..25157
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     27..46
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     111..130
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             complement(423..1571)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(1843..2214)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSL*AYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            2247..2356
                     /note="incP origin of transfer"
     rep_origin      2509..3139
                     /label=oriV
                     /note="incP origin of replication"
     rep_origin      3448..4036
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3937..3956
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             18993..19019
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(24461..119)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
ORIGIN
        1 attgtcgcac ctgattgccc gacattatcg cgagcccatt tatacccata taaatcagca
       61 tccatgttgg aatttaatcg cggcctcgag caagacgttt cccgttgaat atggctcata
      121 acaccccttg tattactgtt tatgtaagca gacagtttta ttgttcatga tgatatattt
      181 ttatcttgtg caatgtaaca tcagagattt tgagacacaa cgtggctttc gacttccggc
      241 aagctatacg cgccctagaa ttgtcaattt taatcctctg tttatcggca gttcgtagag
      301 cgcgccgtgc gtcccgagcg atactgagcg aagcaagtgc gtcgagcagt gcccgcttgt
      361 tcctgaaatg ccagtaaagc gctggctgct gaacccccag ccggaactga ccccacaagg
      421 ccctagcgtt tgcaatgcac caggtcatca ttgacccagg cgtgttccac caggccgctg
      481 cctcgcaact cttcgcaggc ttcgccgacc tgctcgcgcc acttcttcac gcgggtggaa
      541 tccgatccgc acatgaggcg gaaggtttcc agcttgagcg ggtacggctc ccggtgcgag
      601 ctgaaatagt cgaacatccg tcgggccgtc ggcgacagct tgcggtactt ctcccatatg
      661 aatttcgtgt agtggtcgcc agcaaacagc acgacgattt cctcgtcgat caggacctgg
      721 caacgggacg ttttcttgcc acggtccagg acgcggaagc ggtgcagcag cgacaccgat
      781 tccaggtgcc caacgcggtc ggacgtgaag cccatcgccg tcgcctgtag gcgcgacagg
      841 cattcctcgg ccttcgtgta ataccggcca ttgatcgacc agcccaggtc ctggcaaagc
      901 tcgtagaacg tgaaggtgat cggctcgccg ataggggtgc gcttcgcgta ctccaacacc
      961 tgctgccaca ccagttcgtc atcgtcggcc cgcagctcga cgccggtgta ggtgatcttc
     1021 acgtccttgt tgacgtggaa aatgaccttg ttttgcagcg cctcgcgcgg gattttcttg
     1081 ttgcgcgtgg tgaacagggc agagcgggcc gtgtcgtttg gcatcgctcg catcgtgtcc
     1141 ggccacggcg caatatcgaa caaggaaagc tgcatttcct tgatctgctg cttcgtgtgt
     1201 ttcagcaacg cggcctgctt ggcctcgctg acctgttttg ccaggtcctc gccggcggtt
     1261 tttcgcttct tggtcgtcat agttcctcgc gtgtcgatgg tcatcgactt cgccaaacct
     1321 gccgcctcct gttcgagacg acgcgaacgc tccacggcgg ccgatggcgc gggcagggca
     1381 gggggagcca gttgcacgct gtcgcgctcg atcttggccg tagcttgctg gaccatcgag
     1441 ccgacggact ggaaggtttc gcggggcgca cgcatgacgg tgcggcttgc gatggtttcg
     1501 gcatcctcgg cggaaaaccc cgcgtcgatc agttcttgcc tgtatgcctt ccggtcaaac
     1561 gtccgattca ttcaccctcc ttgcgggatt gccccgactc acgccggggc aatgtgccct
     1621 tattcctgat ttgacccgcc tggtgccttg gtgtccagat aatccacctt atcggcaatg
     1681 aagtcggtcc cgtagaccgt ctggccgtcc ttctcgtact tggtattccg aatcttgccc
     1741 tgcacgaata ccagctccgc gaagtcgctc ttcttgatgg agcgcatggg gacgtgcttg
     1801 gcaatcacgc gcaccccccg gccgttttag cggctaaaaa agtcatggct ctgccctcgg
     1861 gcggaccacg cccatcatga ccttgccaag ctcgtcctgc ttctcttcga tcttcgccag
     1921 cagggcgagg atcgtggcat caccgaaccg cgccgtgcgc gggtcgtcgg tgagccagag
     1981 tttcagcagg ccgcccaggc ggcccaggtc gccattgatg cgggccagct cgcggacgtg
     2041 ctcatagtcc acgacgcccg tgattttgta gccctggccg acggccagca ggtaggccta
     2101 caggctcatg ccggccgccg ccgccttttc ctcaatcgct cttcgttcgt ctggaaggca
     2161 gtacaccttg ataggtgggc tgcccttcct ggttggcttg gtttcatcag ccatccgctt
     2221 gccctcatct gttacgccgg cggtagccgg ccagcctcgc agagcaggat tcccgttgag
     2281 caccgccagg tgcgaataag ggacagtgaa gaaggaacac ccgctcgcgg gtgggcctac
     2341 ttcacctatc ctgcccggct gacgccgttg gatacaccaa ggaaagtcta cacgaaccct
     2401 ttggcaaaat cctgtatatc gtgcgaaaaa ggatggatat accgaaaaaa tcgctataat
     2461 gaccccgaag cagggttatg cagcggaaaa gatccgtcga ccctttccga cgctcaccgg
     2521 gctggttgcc ctcgccgctg ggctggcggc cgtctatggc cctgcaaacg cgccagaaac
     2581 gccgtcgaag ccgtgtgcga gacaccgcgg ccgccggcgt tgtggatacc tcgcggaaaa
     2641 cttggccctc actgacagat gaggggcgga cgttgacact tgaggggccg actcacccgg
     2701 cgcggcgttg acagatgagg ggcaggctcg atttcggccg gcgacgtgga gctggccagc
     2761 ctcgcaaatc ggcgaaaacg cctgatttta cgcgagtttt ccacagatga tgtggacaag
     2821 cctggggata agtgccctgc ggtattgaca cttgaggggc gcgactactg acagatgagg
     2881 ggcgcgatcc ttgacacttg aggggcggag tgctgacaga tgaggggcgc acctattgac
     2941 atttgagggg ctgtccacag gcagaaaatc cagcatttgc aagggtttcc gcccgttttt
     3001 cggccaccgc taacctgtct tttaacctgc ttttaaacca atatttataa accttgtttt
     3061 aaccagggct gcgccctgtg cgcgtgaccg cgcacgccga aggggggtgc ccccccttct
     3121 cgaaccctcc cggcccgcta acgcgggcct cccatccccc caggggctgc gcccctcggc
     3181 cgcgaacggc ctcaccccaa aaatggcagc caagctgacc cgctagggac gtgaagtcga
     3241 ttccttcgat ggttagcaat caaagactca gagtgctgtc tggagcgtga atctaacggt
     3301 acgtatctcg attgctcggt cgctattcgc actctgcgaa agttcgtacc gctcattcac
     3361 taggttgcga atcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     3421 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     3481 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     3541 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     3601 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     3661 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     3721 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     3781 cacacagccc agcttgkagc gaacgaccta caccgaactg agatacctac agcgtgagct
     3841 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     3901 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     3961 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     4021 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     4081 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     4141 cgcctttgag tgagctgtcg cgaatgcatc tagatataat tacatcttca tcattcggta
     4201 ttttaaatga catatatcct ccttccgaat agttttaatg gtgatgttcg attggacagt
     4261 gttttccatt attaaaattt taagtatcac atcatgttta atgaaggatt gatgtttatt
     4321 agaatattca taatagcttt gtctttgcct ctatgtaaat gaaattgcat aaaaagcaaa
     4381 ttgcattatt ggcaatgtac ttattctgga tttttcggca cgataaatta ttggtgtaag
     4441 ttttatatca tgagtaaaaa ttcggaatga tattcctgtc attttgtggc ggtagcgtta
     4501 ctcaagatat tatcctacat catatgttga ttattgtaat caatcgagct attttttgtc
     4561 taaaaagggg cttttatgtc tacaagtaca tctcaaattg cggttgaata tcctattcct
     4621 gtctatcgct ttattgtttc tgtcggagat gagaaaattc catttaatag tgtttcagga
     4681 ttagatatta gttatgacac cattgaatac cgagatggtg ttggtaattg gttcaaaatg
     4741 ccgggtcaga gtcagagcac taatatcacc ttgcgtaaag gcgttttccc ggggaaaaca
     4801 gaactgtttg attggattaa ctctattcag cttaatcagg tagagaaaaa ggatattacc
     4861 atcagtttaa ctaatgatgc aggtaccgaa ttattaatga cctggaatgt ttctaatgct
     4921 tttcccactt cattgacttc accttcattt gatgccacca gtaatgatat tgcagtacag
     4981 gaaattacgc tgatggcaga tcgggtgatt atgcaggctg tttgaagcat tgatatttaa
     5041 tcatctcata taagggaact tttatgacaa ccgttaccag ttatcctggc gtttatattg
     5101 aagaattaaa tagcctggcc ttgtcagttt caaatagcgc cacagcggtt cctgtttttg
     5161 ctgtggacga acaaaaccaa tatattagtg aagataatgc aatccgtatt aattcgtgga
     5221 tggattatct taatctgatt ggcaatttta ataatgaaga caaattagat gtttctgtgc
     5281 gtgcttattt tgccaatgga ggtggatatt gttatctcgt caaaacaacg agtttagaaa
     5341 aaattattcc aaccttggat gatgtaacct tattggttgc tgcgggcgaa gatattaaaa
     5401 cgacagtaga tgttttatgt cagccaggaa aagggttatt cgcagtcttt gatggccctg
     5461 aaacagagtt gactatcaac ggtgcggaag aggcaaaaca agcctatacc gccacaccat
     5521 tcgctgcggt ttattatcct tggttgaaag cggattgggc taacatagat attccaccca
     5581 gtgcagtgat ggcgggagtt tatgcatcgg tggatttatc ccgtggtgta tggaaagcgc
     5641 ctgccaatgt tgcgttgaaa gggggcctgg aacctaaatt tttagtcacg gatgaattgc
     5701 agggtgaata taacactggc cgcgctatca atatgattcg taatttcagt aacacaggta
     5761 ctacggtttg gggtgcaaga accctggaag ataaagacaa ttggcgttat gttccagtgc
     5821 gacgcttgtt taattctgtg gagcgggata tcaagcgtgc catgagcttt gctatgttcg
     5881 agcctaataa tcagcctact tgggagcggg tacgggcggc gattagcaac tacctttata
     5941 gcctgtggca acagggggga ttagctggca gcaaagaaga agacgcttat tttgtgcaaa
     6001 ttggtaaagg tataacgatg acacaggagc agattgatgc agggcaaatg attgttaaag
     6061 tcggtttggc tgctgtacgg cctgcggaat ttatcattct ccagtttacg caagatgtag
     6121 aacagcgtta atcatatgat tatgaggagt tatcatgtct gctattctga aagcgcctgg
     6181 cgtttatatt gaagaagacg cttccctagc gttgtctgtc agtaacagcg cgactgccgt
     6241 gcctgttttt atcggaaaat ttactccgac agtggttgat tcaatccaag tctgtacccg
     6301 tatcagcaac tggcttgaat tcacttcctc tttttcccta gctccaacag ttgagattgt
     6361 tgtccaatct aacactgaat ctgaatctga atctgaaact taccactata ttgagacaat
     6421 caatttatct ccagctgtgg aagcattgcg actctatttt caaaatggcg gaggagcttg
     6481 ctatatctac ccattaaatg atgctgaaga tgaattggtt ctggcggcca taccagaagt
     6541 cattgaacag aaaggtgata ttactctgtt ggtttgcccg gaactcgatc tggattacaa
     6601 aactaagatc tatggcgcag tgagctcact gttgaatgat aacaaagtgg gctatttcct
     6661 gattgcggat agcaatgatg gagaatctgt gtcaggagta tggaatagtg ctaaggccgc
     6721 cgcctattat ccccagttgg aaactaacct aaaattttcc acgttgcctg gggataagga
     6781 cattcgtatc agcggttatc aggatgatga tgaaacacat aaaccgaaaa acttggatga
     6841 gctcaggaca atcaacgagg cgttggcaca ggatattgat gcaagattgc tcgaggagaa
     6901 acaacgtgct gtcatcattc cgccaagtgc tgccattgcg ggcatttatt gccaaacgga
     6961 taatcgtcgc ggtgtttgga aagcgccagc caacgttgcg ctcacaggga tcgggagttt
     7021 gcttgataag gtagacgatg aacggcaggg agagatgaat gacaagggaa tcaatgtcat
     7081 ccgttcattt accgaccgtg gttttatggt ctggggagcc cgtacttgtg tggacgctgc
     7141 caacatcagc tggcgttata ttcctgttcg tcgcctgttc aattccgttg aacgagatat
     7201 ccgccaggcg ctgcgcgctg tgttgtttga aactaatagt cagcctacct gggtacgtgc
     7261 taaggctgcc gttgatcaat atctttatac cctttggcag aaaaatgcat tgatgggtgc
     7321 tcgcccggaa gaagcttatt ttgtgcaaat tggtcaggat atcaccatgt ccgaggctga
     7381 tattaaacag ggtaagatga tcatgactgt tggtttggca gcagtgcggc cagctgagtt
     7441 catcattctg caatttacgc aggatgttgt tcagtaatct ccatgactaa acgccaggca
     7501 ctgtattgac agtgcctact ctaaccatct tggaggaggt gatgatgatg gagagactcc
     7561 aaccgggtgt gactttaaca gaaagtataa tcacgatggg tcagcaagag atacccagtg
     7621 ctgtgccggt gtttattggt tacaccgttc gttatccgga acaatcggaa gcatcagtcc
     7681 gtatcgacag tttggccgag tataccagcc tgtttggtga cgaccatgtg atgatgtttg
     7741 ctgtcaggca ctattttgat aatggcgggc aacaggcatt tgttttaccc ctgaaggaca
     7801 atatgccatc agtggagatg accacagctg aagcggaaaa tctgatagcc gcattgcgct
     7861 ctgctacggt tagcgaagcc attggtgggc atagtcagat tacactgatt ttggtaccgg
     7921 atatggctcg gcttaatgac agtgatattg atgactcctc aacccaggta agcctgtggt
     7981 cccaaggctg ggaggcgctg ctgcaattga gtcaggttag gcccaacctc tttgtgctgt
     8041 tagatgcgcc ggataatgtt gaacaggcgc agaagtgtat gacaacgcta tcgtcagatt
     8101 atcgtcaatg gggggcagca tattggcctc gtctggaaac tacctatcag aaagaaatat
     8161 ctggcaagga caatgaatct cagggaattt tccaggggac tgttctgtca cccacagccg
     8221 cggtcgcagc ggtaattcaa cgcacggata acgacgcggg tgtttggaaa gcaccggcca
     8281 atattgcctt atcccaggtt attcgacctg ttaaatctta tcttcaggga agtgtactgt
     8341 ttaacagcag cggcacttcg ctcaatgtga tccgcagttt cccaggtaag ggcatacggg
     8401 tatggggatg ccgcactctg gaaaacacgg ataatacgca gtggcgctat ctgcaaacac
     8461 gtcggctggt ttcctatgta acagcgcatt tgacccaatt ggctcgcatg tatgtcttcg
     8521 agccaaataa tgaacttacc tggatgaagt taaaaggaca aagttacaac tggttacggc
     8581 aattatggtt gcagggtggc ttgtatggtt cacaggagga tgaggcattt aacattctgt
     8641 taggcgtaaa cgagacgatg actgaggatg atgttcgtgc aggaaaaatg atcatgaaag
     8701 ttgagttggc tgtgttgttt cctgccgaat ttattgagat cagtttggtg tttaataccc
     8761 aaacagaggc gctgtcttaa gaaggaaaaa gtacgatgaa cgattattac acacccgtgg
     8821 tatcccatcg ttttatggcg agttttattt ttaaccgcat tcccgatccg ctggatattc
     8881 gttttcagcg tatctctggc cttagtcggg aactacaggt gactcagtac agtgagggag
     8941 gagaaaatgc ccgtaataac tatttagctg agaaaatcca acacggtacg ttgactttgg
     9001 aacggggcgt gatgacagtc tcgccattga cctggatgtt tgatcgggta ttgagtggtg
     9061 aaaaaatcgc ttatgccgat gtggtggtga tgctactgaa tgaaaattca ctgccattgt
     9121 ccagttggac gttgagcaat gcgctgccgg tacgctggca aaccagcgac tttgacgcta
     9181 acagcaatgc catattggtg aatacccttg aattgcgtta ccaggatatg cgctggcttg
     9241 gagtcaaaat atgacagtag aaatcagaga gttacttatc caggcaaagg tagtgccatc
     9301 aacacgaccg actgaatcag aacggcaaaa ccattctttg atacaggaaa gtctggatga
     9361 ggcgacttgg gtggaaacga taaaacgcga agtgttggcc gcattacgcg atgaggaagg
     9421 gtggcgtcca tgagtctgat tgaacgtggt ttagctaagc tgacaattaa tgcttataag
     9481 gatagggaag ggaagatacg ggcaggaacg ttgcaggcca tgtataaccc tgactccttg
     9541 caactggatt accaaacgga ttatcagcaa tcccaagcga ttaatagcga aaagcaaagt
     9601 agcatttatg tacaggccaa gcccgcaggg ttatcacttg aattaatttt tgatgccacg
     9661 atgccgggta acaaaacccc cattgaagag cagctcatgc agctcaagca actgtgcagt
     9721 gtggatgcaa ccagtaacga gacgcgattc ctgcaagtta aatggggcaa aatgcgttgg
     9781 gaaagtcggg gttactttgc tggcagggcc aagagtttgt ctgtgaatta cactttgttt
     9841 gatcgtgatg cgactccctt gagggtacgg gtaatattgg cattagtggc tgatgaaagt
     9901 ctggtgttgc aggagactga acaaaatctg caatctccgg caaaaatcgc attacgcata
     9961 caggatgggg tatctctggc tctgatggca gccagtacgg catcaacatt gtcaggcggt
    10021 gtggattatc tgacgctggc ctggcaaaac ggtctggata atctcaatgg gttcgttccg
    10081 ggtgaaatat tgcaggccac caggggagac gaatcatgag ccaccaactg aaaattattg
    10141 cagatggtaa ggcactgtca cttttggccg cggtagatgt ggacacctgt tatcgggtta
    10201 acagtatacc ttctgcgaca ttgaaactga gcgtaccgga taggccactc tcttctttca
    10261 gtcagacgga tgttcagaca gaactggccc actgtcaggt agggaaaacc ctgcgtctgg
    10321 aattgattga tggtagcaaa aaatgggtgc tgtttaatgg tcttattacc cgtaaggctc
    10381 tgagaattaa gaataagcaa ttattgctca ctctggttgt caagcatcgg ttgcaactga
    10441 tggtggatac ccagcattca cagctgttta aagacaaaag cgaaaaagcg atcttaagca
    10501 cgctattgaa tcagaccgga atcaatgctc gcttcggaaa gatagcggcg ttagatcaaa
    10561 agcatgaaca gatggtgcaa tttcgttgtt cagactggca ttttctgttg tgccgactgt
    10621 cggcaaccgg tgcatggttg ttacctgcca tagaagacgt tcagtttgtt caacctgatg
    10681 ctctgaaatc aaactcagcc tataccttga agagcagggg ggatgagaac aaagacatcg
    10741 ttgtcaagga tgcttactgg cagtttgaca atcaaatcaa ccccgctttg ctggaagtca
    10801 gtggctggga tatcagtaag cagcaggtac aatcaggcgg tcgctacgga aaaatcgcgt
    10861 tgggtaaggc ggcactctct cctgatggat tggcatccct taataaaacg ggttgggaca
    10921 tttgttatag cagtccgtta acaacccagg aaagcggtta tctggcacag ggattattgc
    10981 ttaaccagcg catttctggg gtgacaggag aatttttgct caaaggagat gggcgttacc
    11041 agttgggaga caacattcag ctgactggat ttggttcaca gttagatggt acggcaagca
    11101 ttactgaggt tcgccaccgt cttaatcggc gaattgattg ggaaaccacg gtgagcattg
    11161 gtttacaaca tgaatatttg ccgatattac ctgatgctcc cgaactacat attgcgacag
    11221 tagcgaaata tcagcaggac agtgcggtgt taaaccgtat ccccattatt ctgccggtac
    11281 tgaatcgtcc caatgaattt ttgtgggcca gattggggaa accttatgct agccatgaaa
    11341 gcggtttctg tttttaccca gagccaggtg acgaagttat tattggtttt tttgaaaatg
    11401 atccgcgtta tccagttatt ttaggtgcta tgcataatcc gaaaaataag gccccttttg
    11461 aaccaaccca agataatagg gaaaaagtat tgatcgttaa aaaaggtgaa gcgcaacaac
    11521 aattagtcat tgatggcaaa gagaaaatga tccgaattaa tgcgggtgaa aatcaaataa
    11581 tgcttcagca agataaagac atttctctgt caacgaaaaa agaattaaca ctgaaagcgc
    11641 agacaatgaa tgccacgatg gataaatcat tggcaatgtc cgggaaaaac agtgttgaaa
    11701 tcaaaggcgc aaaaattaat cttacccaat gaaaggtgac gatgaatgga aaatcaaata
    11761 ctgacacaac tctatggtcg tggttgggct tttcctccgg tcttttccct tgaaaagggg
    11821 gtagagatgg ctgaaggggc ggaagatgtg agacaaagtt tgcagattct gtttagtact
    11881 gagccggggg aacgtcttat gcgtgaaaat tatggctgcg gattaaatga ttttatgttt
    11941 gaaaatatcc gcaatgaact tattgctgaa attgaatccc atatccatga caacgtatta
    12001 cgatatgaac cccgggctga tatgactgat attcaggttc gtcaatcccc tggcatgggg
    12061 aatactttgc aagtgcaggt catgtatcgc ctgagaggga gtgatatcaa tcaacaaatc
    12121 cagggagtac ttgcactgag tgaaggccgg gtgacggagg tagtatgagt gaagcgattg
    12181 tggtggatgg tgacgtgtta cagtttgatc ccaactttgg caatcggcag gtgacggttc
    12241 ccagcccagg aaaaattagc ggcacaggac atgcgcaggt aagtggaaaa aaagtgtgta
    12301 ttctggggga tgagaaacag gtcagggttt ctgcaaccta tattacaaca acacatacta
    12361 cgccgggaac aggaaccatt actatcagtg ctctggatgc tggccagcag gcccttcagt
    12421 gtaccagtgg ggcggcttta attatcaagg ggcagcaatt tacggcgatg tttacgcctg
    12481 aattgccagc catgaataat acagtgactc cgccacaacc ggatgttacg acaccttcat
    12541 caggaaaagg acgttttatc actcaacaaa attttgctac cgtaaattag agtattgact
    12601 gaattaaata gaattaacga aggtgtaaat aattatttat ttgctgacga atcgctgtga
    12661 caaataaaca caggtgatgt tatggaatta aatgagttaa ctaacaaatt gtcaaatttg
    12721 gtgccaatga ccgattttaa attagataat cgagccagtt tgcaattgct taaatatatt
    12781 gaagcgtata cgaagataat accctttaat tctggcgata aatattggaa tgactttttc
    12841 tttatgtcag gaaatacgcc agagaaactt gcaaaattat atcagaaaga aatagaaccc
    12901 aatggggagt tattacctca gcaggctttt ttgttggcgg ttttgcgttt attggaaaca
    12961 ccaatatcct tattaaatgt attacctgct gctcatcgtg agctctatta tcgggagctt
    13021 ttaggcttgt cttcccatgc ggcacagcct gatcaggttg ctttatctat ggaactgaat
    13081 tcgacagtga tggaacagct gctccctgaa ggaaccctgt ttgaggctgg tcaggatgaa
    13141 caaggcaatg cattgcaata tgccctggat gccagtttgc tggctaatcg tggatatatc
    13201 agtgacttgc gctggttacg gaatgacggg gaaaagcaat gggttacttc tgctccatgg
    13261 gatttacagg cacaggtgtc actgccgtct gatgggatac gattatttgg taagacaaat
    13321 agtgatcagc aggtatttgg tggggtgttg ataacgtcat cacttctggc gatggaagcg
    13381 gggataagga agatcattgt tacttttgag caggagatga acacccaaga actggtggca
    13441 caggtcagca gtggaaatca atggctaaca ttgacgtctg aggtaaataa gaaagaggtc
    13501 acactgacac tgtcagacaa agaaccggca atcagtgcgc cagaggatct ggataatctc
    13561 tttttcacgc aaccggtact caggctacag ggaaaggata gtcaggcact gccggaggtg
    13621 acgggtatca gcgtttcgga aaaggatgat actaaggata cctcttttga gatgtatcac
    13681 ttaacaccat ttggttatag cagtgatata gagccattgg aggaaaatcc agcgttatat
    13741 ttaggcttta ctgatgtaaa gccagggcaa acactggcgc tgtattggaa attaaaatcc
    13801 ccgcagcaac caaccgtttc ctggtattac ctggatcaac ataatcaatg ggctgaattg
    13861 gattcatggg tcagtgatgg aacccagaat ctgtatcagg atggtacttg gcacgttgag
    13921 ttgcctgtgg atgcatccaa tcaggcagag cagatgccag ttggacgcta ttggttgcgg
    13981 gcagtggtgg aggtacccgc tcatgagggg gcgttgggga aggctccttg gctatatggt
    14041 ctaatctata acgccatgac ggcaaccttg gttaatgtag atagcatcag tgacagccat
    14101 ttcttaaccc ctttgcctgc cagcagcata cagcggcccg ttgaacccat cattgtgttg
    14161 gcatcggtca accagccttg ggcatcatgg ggtggacgta tacctgaatc ctacagtgcc
    14221 ttttttgaac ggatagctca aaacctgtct catcgaaacc ggtccttaac ctggggaaat
    14281 atggtgacat tactcaaaga gcgttatgtc agcatctttg atgttaagta tccaggtaat
    14341 gatgaactca ccagagtgcc agcattggag cagcagcaac taacagtgat tccagcaaac
    14401 cggtacaacg atagcgatga ttctctgcgt ccggtactga atcctgctcg tctgcaagag
    14461 atggctgatt ggttgcagca gaaagactct ccctgggcct ctattgaggt caggaatcca
    14521 gaatacttgg atgtgaaaat ccattacgag gtgattttta aacctgatgt gaacgaagat
    14581 tttggctatc gccagctaca gcagcaactg tgtgaggtgt atatgccttg gagcatagat
    14641 gagcagcggc ccgttgtatt gaataacagc attaattatt tccagttgtt agccactatt
    14701 caacagcaac cgctggttga gcgagtcact cgtctgacac tacatcgggc tgattcttct
    14761 gatgagagtg atggtacagc atctgtggaa gccaaagata atgaagtgct tattttagtc
    14821 tgggaagagg acgataatct gcaataccga ggaaatgact atgagtaatc aggatgcact
    14881 gtttcatagc gttaaagacg atattcactt tgataccttg ctggaacaag ctcatcaggt
    14941 gattgaaaaa caggctgaaa aactgtggag tgatacggca gagcatgatc cgggtatcac
    15001 atttttgcag ggaatcagtt acggtgtgtc agatttggct taccgacata cattacccct
    15061 gaaagattta ctgactccgg cgccggatga gcagcagcaa gagggaattt ttcctgccga
    15121 atttggcccg cataatacac tgacttgtgg gccggtgaca gcggatgatt atcgcaaggc
    15181 attgttagat ctacacagca gcgacagcct ggatggtact cagcaggatg agggggattt
    15241 tctgttccgg agtgtgcaac tggtgcgtga accggaaaaa cagcgttata cctattggta
    15301 tgatgcaacc aagagggaat atagctttgt caacagtgaa ggggctaaag agtttacctt
    15361 gcgggggaat tactggttgt atctggaacc aacccgttgg actcagggta atattgccgc
    15421 tgctaccaga caactgacag aatttttgac taaaaatcgc aatattggtg aatctgtcag
    15481 caacattatc tggctacaac cggttgatct gccactgttg ctggatgttg aactggatga
    15541 tgatgtaggt gcacaggatg tccccggtat ttttgcggcg gtgtatagca ccgcagagca
    15601 gtatctgatg cctggagcac agcgttaccg tacggaagta ctgcaaaatg ctgggatgag
    15661 caatgatcaa atcttcgaag gtccattatt ggaacatggc tggataccag agctgccggc
    15721 agcccgtgat tatactcaaa ggctcactct caatcttagc cggttggtaa atagtctgct
    15781 tgagattgag ggcattaaac atgtgaatcg tcttcgtctg gatgatagct tcgataaaac
    15841 tgctattgaa cccgttaagg gggatacctg gtcgtggtcg atcaaagagg gctattatcc
    15901 acgtctttgg ggagaagacc cacttaacca attggcgcaa caaaatggcc cgcttagggt
    15961 gatagccaaa ggagggatta gcgtcagtgt gagtaaagag caaatccagg ccagtttacc
    16021 cagtcaatca ctgattcaaa atgagccggt aatattggct tacggccagc accgtgacgt
    16081 tggcagctat tatcccgtca gtgatacttt gccgccttgc tatggactac aacattcttt
    16141 gtctgaaagt gaacacttat tgccacttca tcaatttatg ttgccatttg aacaattatt
    16201 ggcctgtggt tgtcaacaga tagccatgct cccgcggtta ctggcttttc agcgcgaagg
    16261 ttatgaggtt tggggtgatc agtggccctt taagtcaggc tcagtgaatg atgacgccca
    16321 tcaagattat gcccctgcat taaaggattt gttaggacag attgcgctgg atagtgatca
    16381 tgaattggat attattaatt acttgctggg ttactttggc acacagcggg caccgcgtac
    16441 ctttacgaca caactcgatg attttcgtgc ggtccaacag ggttatctgg cccagcaacc
    16501 gacattgact taccaccgct ccaatattcg tatcgatcag gtatcgtcgc tacaaaaacg
    16561 tattgctgct cgcatggggc tgggcggtga gttgtttaaa cctcaaccgg atctgagcca
    16621 actgcctttt tatttgattg aacatcgagc gttgctgcca gtcaaaccca atagtcagtt
    16681 tgataaggaa cagaaaccag cctcggtgac agaggagggg ggcagccaaa caggtcaaca
    16741 ttatgtggtc attgaacaga agggcattga tggcaagctg acacaggggc aagtgatcaa
    16801 tttaattctg tatgaaggag agcagggaga aacccaattt acgatacgcg gtcagatggt
    16861 attcaaaacc gagggggata agttttggtt ggatgtgaat aatagtgcgc aactggaata
    16921 taatctggcg cgggtaatga cagcagccaa ggcgagtaaa ctcttttggc aaaacagccc
    16981 ggtatggatg gaggatatgg gctatcgtct ggcctatgct agtgaccaat cctcattgcc
    17041 tgtgaatcaa cggcgcttga cccgcacagt gcaaactcca ttcccgccga tggttgttgt
    17101 aggtagcgaa atcaccctgt taaagcaggt ggggatagtc aatttaaaaa aagcggagtc
    17161 agaaaaactt tatgcaaaag ttgttagctt tgatcgcatt gaagggacct tgattattga
    17221 gcgtttgggt aattccactc tggcttttcc tacctcggaa gaggcgtggc ggtatagttg
    17281 gtatttttcg ggggagaaat atgaaaggac tgaccgcttt tcatttgtga ttagcgtagt
    17341 agtgaacagt gacttaatta aattgcccgg tgttgatccc tataaattgg aagaatgggt
    17401 gaaagaaacg attcttaccg aatttccagc tcatatttct atgattatcc attggatgga
    17461 tcgggaagcc tttttaaatt tcgccaatac ctatcagcgt tggcaaaata atggtacgcc
    17521 actgggggat gcggcttatt ccattctaga aagtttgaca cttggtaaat tgccatctgc
    17581 cttaaaaggt gttggcacaa tgcgtattgc cacatctagt caaagagaag aagtggtggg
    17641 tagtaatggt gatcaatgga atacagatgg aataacccag aatgaattat tctatgttcc
    17701 taaagagagc taggaaaaat aaatatctgc cactaatgat gttgaattaa atatgttttc
    17761 tggagttaat catgaacgaa actcgttata atgcaactgt acaagaacaa caaacattat
    17821 ctaatccaaa agctgttgga cctgacatcg ataaattaaa ggataaattt aaagagggca
    17881 gtattcccct gcaaaccgat ttcaatgagt taattgatat tgccgatatt ggacgtaaag
    17941 cctgtggtca agcgccacaa caaaatggcc caggagaagg attgaaattg gctgatgacg
    18001 gtacgcttaa tttaaaaata ggcacttttt ccaataaaga cttttctcca ttaatattaa
    18061 aagatgatgt tttatctgta gatcttggta gtggtctgac taatgaaacc aatggaatct
    18121 gtgtcggtca gggcgatggt attacagtta acactagcaa tgtagctgta aaacaaggta
    18181 acggaattag cgttactagt agtggtggtg ttgccgttaa agttagtgct aataagggac
    18241 ttagcgttga tagtagtggt gttgcagtta aagttaatac tgataaggga attagcgttg
    18301 atggtaatgg tgttgcagtt aaagttaata ctagtaaagg aattagcgtt gataatacag
    18361 gtgttgcagt tatagctaat gctagtaagg gaattagcgt tgatggtagt ggtgttgcag
    18421 ttatagctaa tactagtaaa ggaattagcg ttgatggtag tggtgttgca gttatagcta
    18481 atactagtaa aggaattagc gttgataata caggtgttgc agttatagct aatgctagta
    18541 agggaattag cgttgatggt agtggtgttg cagttatagc taatactagt aaaggaatta
    18601 gcgttgatgg tagtggtgtt gcagttatag ctaatactag taaaggaatt agcgttgata
    18661 gtagtggtgt tgcagttaaa gttaaagcta atggcggaat taaagtagat gctaatggtg
    18721 ttgcaattga tcctaataat gtactcccca agggagtgat tgtaatgttc tctggcagta
    18781 ctgcaccaac tggttgggcg ttatgtgatg gcaataatgg tacaccaaat ttaatcgatc
    18841 gatttatttt aggtgggaaa gggactgata ttaatggagt gagtactaat acagcttcag
    18901 gtactaaaaa tagtaagtta ttcgatttca gttctgatga agctacatta actattgatg
    18961 gtaaaacact ggggagaggt ggctctggtg gttatccgta tgatgttccg gattatgcag
    19021 gcggttctgg cggccacgat catgatatta agataacggg cacaggaaaa cattctcaca
    19081 aaaacaaagt aacagtccct tattatattc tggctttcat cataaagctt taatatatat
    19141 gaaaaattga aaatataaat tatccattaa taataaagag gatattagca tgacttcgga
    19201 gccaaatctg ttaaaccgga ttacaattac tattgaagct aataatcaac aagtagctag
    19261 aaaagtattg catggctcct tgcttaatca agctaatata aataaattat ttaattcata
    19321 ctttaatgaa tatgaaatta ataggggtgt ttatttagaa acattaatcc tgaatcttgg
    19381 tacgataaat ttccatgatt ttaattcatt gtttcctact ctcctaaaag ctgcattgaa
    19441 taaagaattc agtcaatatc agataaacaa ccatagggaa gaaatgctat ttaatgagac
    19501 aatatcaaat caagctactg ataagtctta catatttggc gataacaaat taattgatgc
    19561 agagaatttc attcactttt tatatcaaaa gcattccaca ttaaatctag tagaagcaat
    19621 gggaaataat ggtattgaaa aattaacaaa tcagttaaca caaatagaaa ataaatttgc
    19681 gttattattg gcaaaaagtt gtttgtctga ggaaggctta aaacgactct tggctatcaa
    19741 acaacccgat ttattaatcg ctatcaatcg cagattatct gaaagaataa atagaccaca
    19801 atatcaggag aagcttgttt cctgcggaca actgatattt agtgctctgg gatatataca
    19861 acagtacaat atacaggaaa ttcctaaacc ggatgaaaaa gttattgcac gcataacaac
    19921 tgaacttaat aataatggtt tgcttaatac aatacctatt attacactat ttcgtcagag
    19981 tgggattaac gattcatcac taaatgattg gctaaagaaa atctggcagg tgagatcaat
    20041 ttcacagtta tgcagaaagt atctttctgc taaggaatac caatatctgt cagaacattt
    20101 tgtttcaaag agcgtcgata aaaatagata tgatgaagag cccgtaaatc agagcatatt
    20161 atcaaggttg aataataatt ccattaaaga aggaaataat cacagtcaac tctgtactct
    20221 cagtagacta tattctgaac ccgttgtatt acctgaacaa accattctac gtcaggttag
    20281 taatacagta gatcagagca tattatcaag gttgaataat gcctccatta aagaaggaaa
    20341 taaccaaagt caacttcgca ctctcagtag actatattct gagcccgttg cattacctga
    20401 acaaaccatt ccacgtcagg ttagtaatac aggtatatta attctatggc caatgctacc
    20461 tacactattt aaccagcttg gtctacttga gaaaaagaaa tttatccatc gtcaggccca
    20521 gtttaatgcc gttgattttc ttgattacct gatttgggga accgaagatg tgaaagtgga
    20581 acgaaaggtt ttgaataatg ttctatgtgg gttaatggct gatgaaatta ctgaaccaat
    20641 gcctattgaa ccagaaaaac aatggataat aattcaatgg ctggacgcta ttatctccca
    20701 actttctggc tggaaaaagt taagtcgtaa tgacgtccgt caattatttc tacaacgacc
    20761 aggagaatta ctgatcaatg aacaggaaat taaaatcaca atacagcaac aaccatttga
    20821 tgctctgtta actgattggc cgtggccaat gaatatggct tgttttagct ggttgagtca
    20881 accattaacc attacgtggt tataaccatt gaccacaatg acttagtctg agtaaaaaat
    20941 atgaatatat cgcctgtttt ttatgattca ttgaatcagg ataacgaccg tgatctatcg
    21001 tttttattta gcgaactgga acgaatagat ctcgctcttc aacaccattt ttattgtgta
    21061 gaaagtcagc gaagtgagct cctggatgag tttctgctca ctgaggcgga agtggtgacc
    21121 aggctggata agccacttgg taaacctcat tggataaatg atgattatct ggcgatatcg
    21181 caaaagggca atgtaagcct aatggcagcg tccagattaa tggatctgat cgaacgcttt
    21241 gaactgactg attttgagcg cgatgtttta ctattaggct tattgcccca ttttgatagc
    21301 cgctattatc gactgttttc gctgattcaa gggggacaac agggtcgatt accttctttt
    21361 gcgctggcat tggaactgtt ttgccactcg gcgctggaga aacaggtaca gcaagcgagt
    21421 tttctgcacc gggcaccttt gatgggttgc cagctattat ccatcgatac tagtcaaaaa
    21481 acgctggcct ggctccagac tccctttatt actgacagcg gggtatatca ctttttactg
    21541 gggcatcact acattatgcc ggctttagaa cattgtgctg agtggttaac accgacaggg
    21601 attggctgtt atcctgaagg attaaaacaa gtactgggta acgtattgtt atctgacaac
    21661 gataatatta gaccgattgt cttattacgg ggaatggccg gcagtgccag agcttatacc
    21721 attactaata tgatggcttc agaagggaag caaacactgc tggtagatat atccaaactt
    21781 gctgatagcg atgaaaaaaa cattattctt cagataaagc atattttgcg ggaaacccgc
    21841 atgcatggag catgtttatt attacggaat ttttgcttgt tagtggaaca gaataaacaa
    21901 ctattggact ccctgtcaga gttattgaat caacctgaat taagaattgt ttgcctgatt
    21961 gagccttatt ccccattggt atggctgaaa aagataccgg tattactgat tgagatgcca
    22021 cttttaacgc ctgcggaaaa agccagattg ttaattgcca gcttaccgga taattgttcc
    22081 gaggatattg atacgataac tttaagccag cgttacactt ttaacccaga aaccctgcca
    22141 ttgattttgc aagaggccca gctttatcaa cagcagcgag atccgctgga tatcttgcag
    22201 caatgcgata tacgccaggc attaaatttg cgtgctcaac aaaatttcgg tcaattggca
    22261 cagcggatta ttcctaagcg ctcattaaag gatttattgg tatccgatga gattgctcag
    22321 cagttacggg aaatactcat agcaattaag tatcgggaac aggttctggc gggagggttt
    22381 aaagataaaa ttgcctatgg cactggtatc agcgccctgt tttatggtga ttcaggcact
    22441 ggaaaaacca tggcagcaga agtgattgct gaccacattg gcgttgactt aataaaagtg
    22501 gatttatcta cagtagtgaa taaatacatc ggtgaaacag aaaaaaactt atcccgtatt
    22561 ttcgatttgg cggaacagga tgcaggggta ttattctttg atgaagctga cgcactgttt
    22621 ggtaaacgca gtgaaactaa agattcccag gacagacatg ccaatattga agtttcttac
    22681 ttattacagc gcctggagaa ttacccgggt ctggtcattt tatccaccaa taatcgtggt
    22741 catttagaca gtgcttttaa tcgtcgtttt actttcatta cccgttttac ttacccggat
    22801 gaaaaaatcc gtaaaaaaat gtggcaggaa atttggccta gaaatataaa aatatcggaa
    22861 gatatcgatt ttaacgaatt agctcaacga acaagcgtga ctggcgcgaa tatccgcaat
    22921 attgctttat tgtcttcatt ctttgcttca gagcagggga atgatgaagt cagtaatgaa
    22981 aatattgaaa ttgcattgaa gcgtgaatta gctaaagtcg gacgattaac attttaaaag
    23041 ttatcacaat gaaagtattg aaatattaaa taaatttatt accaaaaagt tatcacgata
    23101 taatttaaga gaggtttttt atgttaaaca cgcaaactat tattgatgtc aataaggcaa
    23161 tggatgccat gctgcgcgca tatctgaatc aagatattgc cattcgtttt gatctacctg
    23221 aattggatac tatgcaatct gatgcgatgg taagtatctt tctttatgac attcatgaag
    23281 atttacagct tcgctcggca gaatcaagag ggtttgatgt ttatgccggg aggttattgc
    23341 ctggttgggt aaatattaaa tgtaactatc tgattaccta ttgggaagct tctaagccag
    23401 cgactgatgc cagcagtccg gatagccaac ctgataacca ggcaatacaa gtgatgtcac
    23461 aagtattaaa tgccttgatt aataatcgtc aattggcagg tattcctggt gcttatactc
    23521 aggttgtacc gcctaaagag agtttaaata gcctggggaa tttctggcaa tcactgggta
    23581 atcgcccacg gctttctctc aattattcag tgacagtacc tgttagccta aacgatggtc
    23641 aggatagcgc gactccggtt accgcggttt cttctacagt ggaacaaacg gcatcgctca
    23701 gtcaagaagt ggttagtcat gctttacgcg aattactcat tacggaatta ggaggaggag
    23761 aggataaccg gttggtactg agtaaagttg aattatccgc agtgaaagag acgatgactc
    23821 aagacagtcc ggctcagatg attatattgt tgtctgtttc aggcattaca cgacaggaat
    23881 atttgaagga aattgataat atctttgatc gttgggtaaa taatgctgaa gttattacca
    23941 ctattgatga ttgtgggatt agaattgaaa gtataacgaa agataatctt gtaggaattt
    24001 aaattttttt aattaaataa aggaattaat attgatttga tagagcatgt aatcaataac
    24061 ttctatgaaa atgttgaaaa acaatacacg gaaacactta acttacaatt aaattgttaa
    24121 aggagatgat gataagaatg gtcagtcacc agataaaatg atctctggtc aattttagga
    24181 aagctgaaaa aacctgacca aatgcgtcgt aaattctatg cttagaaaga ctcggtgcca
    24241 gtgtaatacc taagatatgg tttttatttc catatctgtt cgaaaactta gaaataacaa
    24301 gttcaacaat aaccagggac tttaaatatc ggatccttgt cgggaagatg cgtgatctga
    24361 tccttcaact cagcaaaagt tcgatttatt caacaaagcc gccgtcccgt caagtcagcg
    24421 taatgctctg ccagtgttac aaccaattaa ccaattctga ttagaaaaac tcatcgagca
    24481 tcaaatgaaa ctgcaattta ttcatatcag gattatcaat accatatttt tgaaaaagcc
    24541 gtttctgtaa tgaaggagaa aactcaccga ggcagttcca taggatggca agatcctggt
    24601 atcggtctgc gattccgact cgtccaacat caatacaacc tattaatttc ccctcgtcaa
    24661 aaataaggtt atcaagtgag aaatcaccat gagtgacgac tgaatccggt gagaatggca
    24721 aaagcttatg catttctttc cagacttgtt caacaggcca gccattacgc tcgtcatcaa
    24781 aatcactcgc atcaaccaaa ccgttattca ttcgtgattg cgcctgagcg agacgaaata
    24841 cgcgatcgct gttaaaagga caattacaaa caggaatcga atgcaaccgg cgcaggaaca
    24901 ctgccagcgc atcaacaata ttttcacctg aatcaggata ttcttctaat acctggaatg
    24961 ctgttttccc ggggatcgca gtggtgagta accatgcatc atcaggagta cggataaaat
    25021 gcttgatggt cggaagaggc ataaattccg tcagccagtt tagtctgacc atctcatctg
    25081 taacatcatt ggcaacgcta cctttgccat gtttcagaaa caactctggc gcatcgggct
    25141 tcccatacaa tcgatag
//

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