质粒名称: | pAWP78-PVCpnf_pvc13-HA |
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目录编号: | 198296 |
存储实验室: | Feng Zhang |
载体骨架基本信息 |
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载体名称: | pAWP78 |
完整质粒大小: | 25157 bp |
载体类型: | Bacterial Expression |
载体抗性: | Kanamycin, 50 μg/mL |
生长温度: | 37 ℃ |
宿主菌: | DH5a |
拷贝数: | 低拷贝 |
插入基因信息 |
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插入基因名称: | PVCpnf_pvc13-HA |
物种来源: | Photorhabdus asymbiotica |
克隆方法: | Gibson Cloning |
文献引用方法 |
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材料和方法部分: | pAWP78-PVCpnf_pvc13-HA was a gift from Feng Zhang (Addgene plasmid # 198296 ; http://n2t.net/addgene:198296 ; RRID:Addgene_198296) |
参考文献部分: | Programmable protein delivery with a bacterial contractile injection system. Kreitz J, Friedrich MJ, Guru A, Lash B, Saito M, Macrae RK, Zhang F. Nature. 2023 Apr;616(7956):357-364. doi: 10.1038/s41586-023-05870-7. Epub 2023 Mar 29. 10.1038/s41586-023-05870-7 PubMed 36991127 |
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载体基本信息
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载体序列
LOCUS pAWP78-PVCpnf_pvc13-HA 25157 bp ds-DNA circular SYN 27-MAR-2023 DEFINITION Produces PVCs retargeted with HA tag. ACCESSION . VERSION . KEYWORDS pAWP78-PVCpnf_pvc13-HA SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 25157) AUTHORS Kreitz J, Friedrich MJ, Guru A, Lash B, Saito M, Macrae RK, Zhang F TITLE Programmable protein delivery with a bacterial contractile injection system JOURNAL Nature March 29, 2023 REFERENCE 2 (bases 1 to 25157) AUTHORS . TITLE Direct Submission JOURNAL Exported Mar 27, 2023 from SnapGene Server 1.1.58 http://www.biofeng.com FEATURES Location/Qualifiers source 1..25157 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 27..46 /label=Kan-R /note="Kanamycin resistance gene, reverse primer" primer_bind 111..130 /label=pENTR-R /note="pENTR vectors, reverse primer" CDS complement(423..1571) /codon_start=1 /product="trans-acting replication protein that binds to and activates oriV" /label=trfA /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR" CDS complement(1843..2214) /codon_start=1 /gene="traJ" /product="oriT-recognizing protein" /label=traJ /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSL*AYLLAV GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE KQDELGKVMMGVVRPRAEP" oriT 2247..2356 /note="incP origin of transfer" rep_origin 2509..3139 /label=oriV /note="incP origin of replication" rep_origin 3448..4036 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 3937..3956 /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS 18993..19019 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /label=HA /translation="YPYDVPDYA" CDS complement(24461..119) /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" ORIGIN 1 attgtcgcac ctgattgccc gacattatcg cgagcccatt tatacccata taaatcagca 61 tccatgttgg aatttaatcg cggcctcgag caagacgttt cccgttgaat atggctcata 121 acaccccttg tattactgtt tatgtaagca gacagtttta ttgttcatga tgatatattt 181 ttatcttgtg caatgtaaca tcagagattt tgagacacaa cgtggctttc gacttccggc 241 aagctatacg cgccctagaa ttgtcaattt taatcctctg tttatcggca gttcgtagag 301 cgcgccgtgc gtcccgagcg atactgagcg aagcaagtgc gtcgagcagt gcccgcttgt 361 tcctgaaatg ccagtaaagc gctggctgct gaacccccag ccggaactga ccccacaagg 421 ccctagcgtt tgcaatgcac caggtcatca ttgacccagg cgtgttccac caggccgctg 481 cctcgcaact cttcgcaggc ttcgccgacc tgctcgcgcc acttcttcac gcgggtggaa 541 tccgatccgc acatgaggcg gaaggtttcc agcttgagcg ggtacggctc ccggtgcgag 601 ctgaaatagt cgaacatccg tcgggccgtc ggcgacagct tgcggtactt ctcccatatg 661 aatttcgtgt agtggtcgcc agcaaacagc acgacgattt cctcgtcgat caggacctgg 721 caacgggacg ttttcttgcc acggtccagg acgcggaagc ggtgcagcag cgacaccgat 781 tccaggtgcc caacgcggtc ggacgtgaag cccatcgccg tcgcctgtag gcgcgacagg 841 cattcctcgg ccttcgtgta ataccggcca ttgatcgacc agcccaggtc ctggcaaagc 901 tcgtagaacg tgaaggtgat cggctcgccg ataggggtgc gcttcgcgta ctccaacacc 961 tgctgccaca ccagttcgtc atcgtcggcc cgcagctcga cgccggtgta ggtgatcttc 1021 acgtccttgt tgacgtggaa aatgaccttg ttttgcagcg cctcgcgcgg gattttcttg 1081 ttgcgcgtgg tgaacagggc agagcgggcc gtgtcgtttg gcatcgctcg catcgtgtcc 1141 ggccacggcg caatatcgaa caaggaaagc tgcatttcct tgatctgctg cttcgtgtgt 1201 ttcagcaacg cggcctgctt ggcctcgctg acctgttttg ccaggtcctc gccggcggtt 1261 tttcgcttct tggtcgtcat agttcctcgc gtgtcgatgg tcatcgactt cgccaaacct 1321 gccgcctcct gttcgagacg acgcgaacgc tccacggcgg ccgatggcgc gggcagggca 1381 gggggagcca gttgcacgct gtcgcgctcg atcttggccg tagcttgctg gaccatcgag 1441 ccgacggact ggaaggtttc gcggggcgca cgcatgacgg tgcggcttgc gatggtttcg 1501 gcatcctcgg cggaaaaccc cgcgtcgatc agttcttgcc tgtatgcctt ccggtcaaac 1561 gtccgattca ttcaccctcc ttgcgggatt gccccgactc acgccggggc aatgtgccct 1621 tattcctgat ttgacccgcc tggtgccttg gtgtccagat aatccacctt atcggcaatg 1681 aagtcggtcc cgtagaccgt ctggccgtcc ttctcgtact tggtattccg aatcttgccc 1741 tgcacgaata ccagctccgc gaagtcgctc ttcttgatgg agcgcatggg gacgtgcttg 1801 gcaatcacgc gcaccccccg gccgttttag cggctaaaaa agtcatggct ctgccctcgg 1861 gcggaccacg cccatcatga ccttgccaag ctcgtcctgc ttctcttcga tcttcgccag 1921 cagggcgagg atcgtggcat caccgaaccg cgccgtgcgc gggtcgtcgg tgagccagag 1981 tttcagcagg ccgcccaggc ggcccaggtc gccattgatg cgggccagct cgcggacgtg 2041 ctcatagtcc acgacgcccg tgattttgta gccctggccg acggccagca ggtaggccta 2101 caggctcatg ccggccgccg ccgccttttc ctcaatcgct cttcgttcgt ctggaaggca 2161 gtacaccttg ataggtgggc tgcccttcct ggttggcttg gtttcatcag ccatccgctt 2221 gccctcatct gttacgccgg cggtagccgg ccagcctcgc agagcaggat tcccgttgag 2281 caccgccagg tgcgaataag ggacagtgaa gaaggaacac ccgctcgcgg gtgggcctac 2341 ttcacctatc ctgcccggct gacgccgttg gatacaccaa ggaaagtcta cacgaaccct 2401 ttggcaaaat cctgtatatc gtgcgaaaaa ggatggatat accgaaaaaa tcgctataat 2461 gaccccgaag cagggttatg cagcggaaaa gatccgtcga ccctttccga cgctcaccgg 2521 gctggttgcc ctcgccgctg ggctggcggc cgtctatggc cctgcaaacg cgccagaaac 2581 gccgtcgaag ccgtgtgcga gacaccgcgg ccgccggcgt tgtggatacc tcgcggaaaa 2641 cttggccctc actgacagat gaggggcgga cgttgacact tgaggggccg actcacccgg 2701 cgcggcgttg acagatgagg ggcaggctcg atttcggccg gcgacgtgga gctggccagc 2761 ctcgcaaatc ggcgaaaacg cctgatttta cgcgagtttt ccacagatga tgtggacaag 2821 cctggggata agtgccctgc ggtattgaca cttgaggggc gcgactactg acagatgagg 2881 ggcgcgatcc ttgacacttg aggggcggag tgctgacaga tgaggggcgc acctattgac 2941 atttgagggg ctgtccacag gcagaaaatc cagcatttgc aagggtttcc gcccgttttt 3001 cggccaccgc taacctgtct tttaacctgc ttttaaacca atatttataa accttgtttt 3061 aaccagggct gcgccctgtg cgcgtgaccg cgcacgccga aggggggtgc ccccccttct 3121 cgaaccctcc cggcccgcta acgcgggcct cccatccccc caggggctgc gcccctcggc 3181 cgcgaacggc ctcaccccaa aaatggcagc caagctgacc cgctagggac gtgaagtcga 3241 ttccttcgat ggttagcaat caaagactca gagtgctgtc tggagcgtga atctaacggt 3301 acgtatctcg attgctcggt cgctattcgc actctgcgaa agttcgtacc gctcattcac 3361 taggttgcga atcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga 3421 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg 3481 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc 3541 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct 3601 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc 3661 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt 3721 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg 3781 cacacagccc agcttgkagc gaacgaccta caccgaactg agatacctac agcgtgagct 3841 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag 3901 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag 3961 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg 4021 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg 4081 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac 4141 cgcctttgag tgagctgtcg cgaatgcatc tagatataat tacatcttca tcattcggta 4201 ttttaaatga catatatcct ccttccgaat agttttaatg gtgatgttcg attggacagt 4261 gttttccatt attaaaattt taagtatcac atcatgttta atgaaggatt gatgtttatt 4321 agaatattca taatagcttt gtctttgcct ctatgtaaat gaaattgcat aaaaagcaaa 4381 ttgcattatt ggcaatgtac ttattctgga tttttcggca cgataaatta ttggtgtaag 4441 ttttatatca tgagtaaaaa ttcggaatga tattcctgtc attttgtggc ggtagcgtta 4501 ctcaagatat tatcctacat catatgttga ttattgtaat caatcgagct attttttgtc 4561 taaaaagggg cttttatgtc tacaagtaca tctcaaattg cggttgaata tcctattcct 4621 gtctatcgct ttattgtttc tgtcggagat gagaaaattc catttaatag tgtttcagga 4681 ttagatatta gttatgacac cattgaatac cgagatggtg ttggtaattg gttcaaaatg 4741 ccgggtcaga gtcagagcac taatatcacc ttgcgtaaag gcgttttccc ggggaaaaca 4801 gaactgtttg attggattaa ctctattcag cttaatcagg tagagaaaaa ggatattacc 4861 atcagtttaa ctaatgatgc aggtaccgaa ttattaatga cctggaatgt ttctaatgct 4921 tttcccactt cattgacttc accttcattt gatgccacca gtaatgatat tgcagtacag 4981 gaaattacgc tgatggcaga tcgggtgatt atgcaggctg tttgaagcat tgatatttaa 5041 tcatctcata taagggaact tttatgacaa ccgttaccag ttatcctggc gtttatattg 5101 aagaattaaa tagcctggcc ttgtcagttt caaatagcgc cacagcggtt cctgtttttg 5161 ctgtggacga acaaaaccaa tatattagtg aagataatgc aatccgtatt aattcgtgga 5221 tggattatct taatctgatt ggcaatttta ataatgaaga caaattagat gtttctgtgc 5281 gtgcttattt tgccaatgga ggtggatatt gttatctcgt caaaacaacg agtttagaaa 5341 aaattattcc aaccttggat gatgtaacct tattggttgc tgcgggcgaa gatattaaaa 5401 cgacagtaga tgttttatgt cagccaggaa aagggttatt cgcagtcttt gatggccctg 5461 aaacagagtt gactatcaac ggtgcggaag aggcaaaaca agcctatacc gccacaccat 5521 tcgctgcggt ttattatcct tggttgaaag cggattgggc taacatagat attccaccca 5581 gtgcagtgat ggcgggagtt tatgcatcgg tggatttatc ccgtggtgta tggaaagcgc 5641 ctgccaatgt tgcgttgaaa gggggcctgg aacctaaatt tttagtcacg gatgaattgc 5701 agggtgaata taacactggc cgcgctatca atatgattcg taatttcagt aacacaggta 5761 ctacggtttg gggtgcaaga accctggaag ataaagacaa ttggcgttat gttccagtgc 5821 gacgcttgtt taattctgtg gagcgggata tcaagcgtgc catgagcttt gctatgttcg 5881 agcctaataa tcagcctact tgggagcggg tacgggcggc gattagcaac tacctttata 5941 gcctgtggca acagggggga ttagctggca gcaaagaaga agacgcttat tttgtgcaaa 6001 ttggtaaagg tataacgatg acacaggagc agattgatgc agggcaaatg attgttaaag 6061 tcggtttggc tgctgtacgg cctgcggaat ttatcattct ccagtttacg caagatgtag 6121 aacagcgtta atcatatgat tatgaggagt tatcatgtct gctattctga aagcgcctgg 6181 cgtttatatt gaagaagacg cttccctagc gttgtctgtc agtaacagcg cgactgccgt 6241 gcctgttttt atcggaaaat ttactccgac agtggttgat tcaatccaag tctgtacccg 6301 tatcagcaac tggcttgaat tcacttcctc tttttcccta gctccaacag ttgagattgt 6361 tgtccaatct aacactgaat ctgaatctga atctgaaact taccactata ttgagacaat 6421 caatttatct ccagctgtgg aagcattgcg actctatttt caaaatggcg gaggagcttg 6481 ctatatctac ccattaaatg atgctgaaga tgaattggtt ctggcggcca taccagaagt 6541 cattgaacag aaaggtgata ttactctgtt ggtttgcccg gaactcgatc tggattacaa 6601 aactaagatc tatggcgcag tgagctcact gttgaatgat aacaaagtgg gctatttcct 6661 gattgcggat agcaatgatg gagaatctgt gtcaggagta tggaatagtg ctaaggccgc 6721 cgcctattat ccccagttgg aaactaacct aaaattttcc acgttgcctg gggataagga 6781 cattcgtatc agcggttatc aggatgatga tgaaacacat aaaccgaaaa acttggatga 6841 gctcaggaca atcaacgagg cgttggcaca ggatattgat gcaagattgc tcgaggagaa 6901 acaacgtgct gtcatcattc cgccaagtgc tgccattgcg ggcatttatt gccaaacgga 6961 taatcgtcgc ggtgtttgga aagcgccagc caacgttgcg ctcacaggga tcgggagttt 7021 gcttgataag gtagacgatg aacggcaggg agagatgaat gacaagggaa tcaatgtcat 7081 ccgttcattt accgaccgtg gttttatggt ctggggagcc cgtacttgtg tggacgctgc 7141 caacatcagc tggcgttata ttcctgttcg tcgcctgttc aattccgttg aacgagatat 7201 ccgccaggcg ctgcgcgctg tgttgtttga aactaatagt cagcctacct gggtacgtgc 7261 taaggctgcc gttgatcaat atctttatac cctttggcag aaaaatgcat tgatgggtgc 7321 tcgcccggaa gaagcttatt ttgtgcaaat tggtcaggat atcaccatgt ccgaggctga 7381 tattaaacag ggtaagatga tcatgactgt tggtttggca gcagtgcggc cagctgagtt 7441 catcattctg caatttacgc aggatgttgt tcagtaatct ccatgactaa acgccaggca 7501 ctgtattgac agtgcctact ctaaccatct tggaggaggt gatgatgatg gagagactcc 7561 aaccgggtgt gactttaaca gaaagtataa tcacgatggg tcagcaagag atacccagtg 7621 ctgtgccggt gtttattggt tacaccgttc gttatccgga acaatcggaa gcatcagtcc 7681 gtatcgacag tttggccgag tataccagcc tgtttggtga cgaccatgtg atgatgtttg 7741 ctgtcaggca ctattttgat aatggcgggc aacaggcatt tgttttaccc ctgaaggaca 7801 atatgccatc agtggagatg accacagctg aagcggaaaa tctgatagcc gcattgcgct 7861 ctgctacggt tagcgaagcc attggtgggc atagtcagat tacactgatt ttggtaccgg 7921 atatggctcg gcttaatgac agtgatattg atgactcctc aacccaggta agcctgtggt 7981 cccaaggctg ggaggcgctg ctgcaattga gtcaggttag gcccaacctc tttgtgctgt 8041 tagatgcgcc ggataatgtt gaacaggcgc agaagtgtat gacaacgcta tcgtcagatt 8101 atcgtcaatg gggggcagca tattggcctc gtctggaaac tacctatcag aaagaaatat 8161 ctggcaagga caatgaatct cagggaattt tccaggggac tgttctgtca cccacagccg 8221 cggtcgcagc ggtaattcaa cgcacggata acgacgcggg tgtttggaaa gcaccggcca 8281 atattgcctt atcccaggtt attcgacctg ttaaatctta tcttcaggga agtgtactgt 8341 ttaacagcag cggcacttcg ctcaatgtga tccgcagttt cccaggtaag ggcatacggg 8401 tatggggatg ccgcactctg gaaaacacgg ataatacgca gtggcgctat ctgcaaacac 8461 gtcggctggt ttcctatgta acagcgcatt tgacccaatt ggctcgcatg tatgtcttcg 8521 agccaaataa tgaacttacc tggatgaagt taaaaggaca aagttacaac tggttacggc 8581 aattatggtt gcagggtggc ttgtatggtt cacaggagga tgaggcattt aacattctgt 8641 taggcgtaaa cgagacgatg actgaggatg atgttcgtgc aggaaaaatg atcatgaaag 8701 ttgagttggc tgtgttgttt cctgccgaat ttattgagat cagtttggtg tttaataccc 8761 aaacagaggc gctgtcttaa gaaggaaaaa gtacgatgaa cgattattac acacccgtgg 8821 tatcccatcg ttttatggcg agttttattt ttaaccgcat tcccgatccg ctggatattc 8881 gttttcagcg tatctctggc cttagtcggg aactacaggt gactcagtac agtgagggag 8941 gagaaaatgc ccgtaataac tatttagctg agaaaatcca acacggtacg ttgactttgg 9001 aacggggcgt gatgacagtc tcgccattga cctggatgtt tgatcgggta ttgagtggtg 9061 aaaaaatcgc ttatgccgat gtggtggtga tgctactgaa tgaaaattca ctgccattgt 9121 ccagttggac gttgagcaat gcgctgccgg tacgctggca aaccagcgac tttgacgcta 9181 acagcaatgc catattggtg aatacccttg aattgcgtta ccaggatatg cgctggcttg 9241 gagtcaaaat atgacagtag aaatcagaga gttacttatc caggcaaagg tagtgccatc 9301 aacacgaccg actgaatcag aacggcaaaa ccattctttg atacaggaaa gtctggatga 9361 ggcgacttgg gtggaaacga taaaacgcga agtgttggcc gcattacgcg atgaggaagg 9421 gtggcgtcca tgagtctgat tgaacgtggt ttagctaagc tgacaattaa tgcttataag 9481 gatagggaag ggaagatacg ggcaggaacg ttgcaggcca tgtataaccc tgactccttg 9541 caactggatt accaaacgga ttatcagcaa tcccaagcga ttaatagcga aaagcaaagt 9601 agcatttatg tacaggccaa gcccgcaggg ttatcacttg aattaatttt tgatgccacg 9661 atgccgggta acaaaacccc cattgaagag cagctcatgc agctcaagca actgtgcagt 9721 gtggatgcaa ccagtaacga gacgcgattc ctgcaagtta aatggggcaa aatgcgttgg 9781 gaaagtcggg gttactttgc tggcagggcc aagagtttgt ctgtgaatta cactttgttt 9841 gatcgtgatg cgactccctt gagggtacgg gtaatattgg cattagtggc tgatgaaagt 9901 ctggtgttgc aggagactga acaaaatctg caatctccgg caaaaatcgc attacgcata 9961 caggatgggg tatctctggc tctgatggca gccagtacgg catcaacatt gtcaggcggt 10021 gtggattatc tgacgctggc ctggcaaaac ggtctggata atctcaatgg gttcgttccg 10081 ggtgaaatat tgcaggccac caggggagac gaatcatgag ccaccaactg aaaattattg 10141 cagatggtaa ggcactgtca cttttggccg cggtagatgt ggacacctgt tatcgggtta 10201 acagtatacc ttctgcgaca ttgaaactga gcgtaccgga taggccactc tcttctttca 10261 gtcagacgga tgttcagaca gaactggccc actgtcaggt agggaaaacc ctgcgtctgg 10321 aattgattga tggtagcaaa aaatgggtgc tgtttaatgg tcttattacc cgtaaggctc 10381 tgagaattaa gaataagcaa ttattgctca ctctggttgt caagcatcgg ttgcaactga 10441 tggtggatac ccagcattca cagctgttta aagacaaaag cgaaaaagcg atcttaagca 10501 cgctattgaa tcagaccgga atcaatgctc gcttcggaaa gatagcggcg ttagatcaaa 10561 agcatgaaca gatggtgcaa tttcgttgtt cagactggca ttttctgttg tgccgactgt 10621 cggcaaccgg tgcatggttg ttacctgcca tagaagacgt tcagtttgtt caacctgatg 10681 ctctgaaatc aaactcagcc tataccttga agagcagggg ggatgagaac aaagacatcg 10741 ttgtcaagga tgcttactgg cagtttgaca atcaaatcaa ccccgctttg ctggaagtca 10801 gtggctggga tatcagtaag cagcaggtac aatcaggcgg tcgctacgga aaaatcgcgt 10861 tgggtaaggc ggcactctct cctgatggat tggcatccct taataaaacg ggttgggaca 10921 tttgttatag cagtccgtta acaacccagg aaagcggtta tctggcacag ggattattgc 10981 ttaaccagcg catttctggg gtgacaggag aatttttgct caaaggagat gggcgttacc 11041 agttgggaga caacattcag ctgactggat ttggttcaca gttagatggt acggcaagca 11101 ttactgaggt tcgccaccgt cttaatcggc gaattgattg ggaaaccacg gtgagcattg 11161 gtttacaaca tgaatatttg ccgatattac ctgatgctcc cgaactacat attgcgacag 11221 tagcgaaata tcagcaggac agtgcggtgt taaaccgtat ccccattatt ctgccggtac 11281 tgaatcgtcc caatgaattt ttgtgggcca gattggggaa accttatgct agccatgaaa 11341 gcggtttctg tttttaccca gagccaggtg acgaagttat tattggtttt tttgaaaatg 11401 atccgcgtta tccagttatt ttaggtgcta tgcataatcc gaaaaataag gccccttttg 11461 aaccaaccca agataatagg gaaaaagtat tgatcgttaa aaaaggtgaa gcgcaacaac 11521 aattagtcat tgatggcaaa gagaaaatga tccgaattaa tgcgggtgaa aatcaaataa 11581 tgcttcagca agataaagac atttctctgt caacgaaaaa agaattaaca ctgaaagcgc 11641 agacaatgaa tgccacgatg gataaatcat tggcaatgtc cgggaaaaac agtgttgaaa 11701 tcaaaggcgc aaaaattaat cttacccaat gaaaggtgac gatgaatgga aaatcaaata 11761 ctgacacaac tctatggtcg tggttgggct tttcctccgg tcttttccct tgaaaagggg 11821 gtagagatgg ctgaaggggc ggaagatgtg agacaaagtt tgcagattct gtttagtact 11881 gagccggggg aacgtcttat gcgtgaaaat tatggctgcg gattaaatga ttttatgttt 11941 gaaaatatcc gcaatgaact tattgctgaa attgaatccc atatccatga caacgtatta 12001 cgatatgaac cccgggctga tatgactgat attcaggttc gtcaatcccc tggcatgggg 12061 aatactttgc aagtgcaggt catgtatcgc ctgagaggga gtgatatcaa tcaacaaatc 12121 cagggagtac ttgcactgag tgaaggccgg gtgacggagg tagtatgagt gaagcgattg 12181 tggtggatgg tgacgtgtta cagtttgatc ccaactttgg caatcggcag gtgacggttc 12241 ccagcccagg aaaaattagc ggcacaggac atgcgcaggt aagtggaaaa aaagtgtgta 12301 ttctggggga tgagaaacag gtcagggttt ctgcaaccta tattacaaca acacatacta 12361 cgccgggaac aggaaccatt actatcagtg ctctggatgc tggccagcag gcccttcagt 12421 gtaccagtgg ggcggcttta attatcaagg ggcagcaatt tacggcgatg tttacgcctg 12481 aattgccagc catgaataat acagtgactc cgccacaacc ggatgttacg acaccttcat 12541 caggaaaagg acgttttatc actcaacaaa attttgctac cgtaaattag agtattgact 12601 gaattaaata gaattaacga aggtgtaaat aattatttat ttgctgacga atcgctgtga 12661 caaataaaca caggtgatgt tatggaatta aatgagttaa ctaacaaatt gtcaaatttg 12721 gtgccaatga ccgattttaa attagataat cgagccagtt tgcaattgct taaatatatt 12781 gaagcgtata cgaagataat accctttaat tctggcgata aatattggaa tgactttttc 12841 tttatgtcag gaaatacgcc agagaaactt gcaaaattat atcagaaaga aatagaaccc 12901 aatggggagt tattacctca gcaggctttt ttgttggcgg ttttgcgttt attggaaaca 12961 ccaatatcct tattaaatgt attacctgct gctcatcgtg agctctatta tcgggagctt 13021 ttaggcttgt cttcccatgc ggcacagcct gatcaggttg ctttatctat ggaactgaat 13081 tcgacagtga tggaacagct gctccctgaa ggaaccctgt ttgaggctgg tcaggatgaa 13141 caaggcaatg cattgcaata tgccctggat gccagtttgc tggctaatcg tggatatatc 13201 agtgacttgc gctggttacg gaatgacggg gaaaagcaat gggttacttc tgctccatgg 13261 gatttacagg cacaggtgtc actgccgtct gatgggatac gattatttgg taagacaaat 13321 agtgatcagc aggtatttgg tggggtgttg ataacgtcat cacttctggc gatggaagcg 13381 gggataagga agatcattgt tacttttgag caggagatga acacccaaga actggtggca 13441 caggtcagca gtggaaatca atggctaaca ttgacgtctg aggtaaataa gaaagaggtc 13501 acactgacac tgtcagacaa agaaccggca atcagtgcgc cagaggatct ggataatctc 13561 tttttcacgc aaccggtact caggctacag ggaaaggata gtcaggcact gccggaggtg 13621 acgggtatca gcgtttcgga aaaggatgat actaaggata cctcttttga gatgtatcac 13681 ttaacaccat ttggttatag cagtgatata gagccattgg aggaaaatcc agcgttatat 13741 ttaggcttta ctgatgtaaa gccagggcaa acactggcgc tgtattggaa attaaaatcc 13801 ccgcagcaac caaccgtttc ctggtattac ctggatcaac ataatcaatg ggctgaattg 13861 gattcatggg tcagtgatgg aacccagaat ctgtatcagg atggtacttg gcacgttgag 13921 ttgcctgtgg atgcatccaa tcaggcagag cagatgccag ttggacgcta ttggttgcgg 13981 gcagtggtgg aggtacccgc tcatgagggg gcgttgggga aggctccttg gctatatggt 14041 ctaatctata acgccatgac ggcaaccttg gttaatgtag atagcatcag tgacagccat 14101 ttcttaaccc ctttgcctgc cagcagcata cagcggcccg ttgaacccat cattgtgttg 14161 gcatcggtca accagccttg ggcatcatgg ggtggacgta tacctgaatc ctacagtgcc 14221 ttttttgaac ggatagctca aaacctgtct catcgaaacc ggtccttaac ctggggaaat 14281 atggtgacat tactcaaaga gcgttatgtc agcatctttg atgttaagta tccaggtaat 14341 gatgaactca ccagagtgcc agcattggag cagcagcaac taacagtgat tccagcaaac 14401 cggtacaacg atagcgatga ttctctgcgt ccggtactga atcctgctcg tctgcaagag 14461 atggctgatt ggttgcagca gaaagactct ccctgggcct ctattgaggt caggaatcca 14521 gaatacttgg atgtgaaaat ccattacgag gtgattttta aacctgatgt gaacgaagat 14581 tttggctatc gccagctaca gcagcaactg tgtgaggtgt atatgccttg gagcatagat 14641 gagcagcggc ccgttgtatt gaataacagc attaattatt tccagttgtt agccactatt 14701 caacagcaac cgctggttga gcgagtcact cgtctgacac tacatcgggc tgattcttct 14761 gatgagagtg atggtacagc atctgtggaa gccaaagata atgaagtgct tattttagtc 14821 tgggaagagg acgataatct gcaataccga ggaaatgact atgagtaatc aggatgcact 14881 gtttcatagc gttaaagacg atattcactt tgataccttg ctggaacaag ctcatcaggt 14941 gattgaaaaa caggctgaaa aactgtggag tgatacggca gagcatgatc cgggtatcac 15001 atttttgcag ggaatcagtt acggtgtgtc agatttggct taccgacata cattacccct 15061 gaaagattta ctgactccgg cgccggatga gcagcagcaa gagggaattt ttcctgccga 15121 atttggcccg cataatacac tgacttgtgg gccggtgaca gcggatgatt atcgcaaggc 15181 attgttagat ctacacagca gcgacagcct ggatggtact cagcaggatg agggggattt 15241 tctgttccgg agtgtgcaac tggtgcgtga accggaaaaa cagcgttata cctattggta 15301 tgatgcaacc aagagggaat atagctttgt caacagtgaa ggggctaaag agtttacctt 15361 gcgggggaat tactggttgt atctggaacc aacccgttgg actcagggta atattgccgc 15421 tgctaccaga caactgacag aatttttgac taaaaatcgc aatattggtg aatctgtcag 15481 caacattatc tggctacaac cggttgatct gccactgttg ctggatgttg aactggatga 15541 tgatgtaggt gcacaggatg tccccggtat ttttgcggcg gtgtatagca ccgcagagca 15601 gtatctgatg cctggagcac agcgttaccg tacggaagta ctgcaaaatg ctgggatgag 15661 caatgatcaa atcttcgaag gtccattatt ggaacatggc tggataccag agctgccggc 15721 agcccgtgat tatactcaaa ggctcactct caatcttagc cggttggtaa atagtctgct 15781 tgagattgag ggcattaaac atgtgaatcg tcttcgtctg gatgatagct tcgataaaac 15841 tgctattgaa cccgttaagg gggatacctg gtcgtggtcg atcaaagagg gctattatcc 15901 acgtctttgg ggagaagacc cacttaacca attggcgcaa caaaatggcc cgcttagggt 15961 gatagccaaa ggagggatta gcgtcagtgt gagtaaagag caaatccagg ccagtttacc 16021 cagtcaatca ctgattcaaa atgagccggt aatattggct tacggccagc accgtgacgt 16081 tggcagctat tatcccgtca gtgatacttt gccgccttgc tatggactac aacattcttt 16141 gtctgaaagt gaacacttat tgccacttca tcaatttatg ttgccatttg aacaattatt 16201 ggcctgtggt tgtcaacaga tagccatgct cccgcggtta ctggcttttc agcgcgaagg 16261 ttatgaggtt tggggtgatc agtggccctt taagtcaggc tcagtgaatg atgacgccca 16321 tcaagattat gcccctgcat taaaggattt gttaggacag attgcgctgg atagtgatca 16381 tgaattggat attattaatt acttgctggg ttactttggc acacagcggg caccgcgtac 16441 ctttacgaca caactcgatg attttcgtgc ggtccaacag ggttatctgg cccagcaacc 16501 gacattgact taccaccgct ccaatattcg tatcgatcag gtatcgtcgc tacaaaaacg 16561 tattgctgct cgcatggggc tgggcggtga gttgtttaaa cctcaaccgg atctgagcca 16621 actgcctttt tatttgattg aacatcgagc gttgctgcca gtcaaaccca atagtcagtt 16681 tgataaggaa cagaaaccag cctcggtgac agaggagggg ggcagccaaa caggtcaaca 16741 ttatgtggtc attgaacaga agggcattga tggcaagctg acacaggggc aagtgatcaa 16801 tttaattctg tatgaaggag agcagggaga aacccaattt acgatacgcg gtcagatggt 16861 attcaaaacc gagggggata agttttggtt ggatgtgaat aatagtgcgc aactggaata 16921 taatctggcg cgggtaatga cagcagccaa ggcgagtaaa ctcttttggc aaaacagccc 16981 ggtatggatg gaggatatgg gctatcgtct ggcctatgct agtgaccaat cctcattgcc 17041 tgtgaatcaa cggcgcttga cccgcacagt gcaaactcca ttcccgccga tggttgttgt 17101 aggtagcgaa atcaccctgt taaagcaggt ggggatagtc aatttaaaaa aagcggagtc 17161 agaaaaactt tatgcaaaag ttgttagctt tgatcgcatt gaagggacct tgattattga 17221 gcgtttgggt aattccactc tggcttttcc tacctcggaa gaggcgtggc ggtatagttg 17281 gtatttttcg ggggagaaat atgaaaggac tgaccgcttt tcatttgtga ttagcgtagt 17341 agtgaacagt gacttaatta aattgcccgg tgttgatccc tataaattgg aagaatgggt 17401 gaaagaaacg attcttaccg aatttccagc tcatatttct atgattatcc attggatgga 17461 tcgggaagcc tttttaaatt tcgccaatac ctatcagcgt tggcaaaata atggtacgcc 17521 actgggggat gcggcttatt ccattctaga aagtttgaca cttggtaaat tgccatctgc 17581 cttaaaaggt gttggcacaa tgcgtattgc cacatctagt caaagagaag aagtggtggg 17641 tagtaatggt gatcaatgga atacagatgg aataacccag aatgaattat tctatgttcc 17701 taaagagagc taggaaaaat aaatatctgc cactaatgat gttgaattaa atatgttttc 17761 tggagttaat catgaacgaa actcgttata atgcaactgt acaagaacaa caaacattat 17821 ctaatccaaa agctgttgga cctgacatcg ataaattaaa ggataaattt aaagagggca 17881 gtattcccct gcaaaccgat ttcaatgagt taattgatat tgccgatatt ggacgtaaag 17941 cctgtggtca agcgccacaa caaaatggcc caggagaagg attgaaattg gctgatgacg 18001 gtacgcttaa tttaaaaata ggcacttttt ccaataaaga cttttctcca ttaatattaa 18061 aagatgatgt tttatctgta gatcttggta gtggtctgac taatgaaacc aatggaatct 18121 gtgtcggtca gggcgatggt attacagtta acactagcaa tgtagctgta aaacaaggta 18181 acggaattag cgttactagt agtggtggtg ttgccgttaa agttagtgct aataagggac 18241 ttagcgttga tagtagtggt gttgcagtta aagttaatac tgataaggga attagcgttg 18301 atggtaatgg tgttgcagtt aaagttaata ctagtaaagg aattagcgtt gataatacag 18361 gtgttgcagt tatagctaat gctagtaagg gaattagcgt tgatggtagt ggtgttgcag 18421 ttatagctaa tactagtaaa ggaattagcg ttgatggtag tggtgttgca gttatagcta 18481 atactagtaa aggaattagc gttgataata caggtgttgc agttatagct aatgctagta 18541 agggaattag cgttgatggt agtggtgttg cagttatagc taatactagt aaaggaatta 18601 gcgttgatgg tagtggtgtt gcagttatag ctaatactag taaaggaatt agcgttgata 18661 gtagtggtgt tgcagttaaa gttaaagcta atggcggaat taaagtagat gctaatggtg 18721 ttgcaattga tcctaataat gtactcccca agggagtgat tgtaatgttc tctggcagta 18781 ctgcaccaac tggttgggcg ttatgtgatg gcaataatgg tacaccaaat ttaatcgatc 18841 gatttatttt aggtgggaaa gggactgata ttaatggagt gagtactaat acagcttcag 18901 gtactaaaaa tagtaagtta ttcgatttca gttctgatga agctacatta actattgatg 18961 gtaaaacact ggggagaggt ggctctggtg gttatccgta tgatgttccg gattatgcag 19021 gcggttctgg cggccacgat catgatatta agataacggg cacaggaaaa cattctcaca 19081 aaaacaaagt aacagtccct tattatattc tggctttcat cataaagctt taatatatat 19141 gaaaaattga aaatataaat tatccattaa taataaagag gatattagca tgacttcgga 19201 gccaaatctg ttaaaccgga ttacaattac tattgaagct aataatcaac aagtagctag 19261 aaaagtattg catggctcct tgcttaatca agctaatata aataaattat ttaattcata 19321 ctttaatgaa tatgaaatta ataggggtgt ttatttagaa acattaatcc tgaatcttgg 19381 tacgataaat ttccatgatt ttaattcatt gtttcctact ctcctaaaag ctgcattgaa 19441 taaagaattc agtcaatatc agataaacaa ccatagggaa gaaatgctat ttaatgagac 19501 aatatcaaat caagctactg ataagtctta catatttggc gataacaaat taattgatgc 19561 agagaatttc attcactttt tatatcaaaa gcattccaca ttaaatctag tagaagcaat 19621 gggaaataat ggtattgaaa aattaacaaa tcagttaaca caaatagaaa ataaatttgc 19681 gttattattg gcaaaaagtt gtttgtctga ggaaggctta aaacgactct tggctatcaa 19741 acaacccgat ttattaatcg ctatcaatcg cagattatct gaaagaataa atagaccaca 19801 atatcaggag aagcttgttt cctgcggaca actgatattt agtgctctgg gatatataca 19861 acagtacaat atacaggaaa ttcctaaacc ggatgaaaaa gttattgcac gcataacaac 19921 tgaacttaat aataatggtt tgcttaatac aatacctatt attacactat ttcgtcagag 19981 tgggattaac gattcatcac taaatgattg gctaaagaaa atctggcagg tgagatcaat 20041 ttcacagtta tgcagaaagt atctttctgc taaggaatac caatatctgt cagaacattt 20101 tgtttcaaag agcgtcgata aaaatagata tgatgaagag cccgtaaatc agagcatatt 20161 atcaaggttg aataataatt ccattaaaga aggaaataat cacagtcaac tctgtactct 20221 cagtagacta tattctgaac ccgttgtatt acctgaacaa accattctac gtcaggttag 20281 taatacagta gatcagagca tattatcaag gttgaataat gcctccatta aagaaggaaa 20341 taaccaaagt caacttcgca ctctcagtag actatattct gagcccgttg cattacctga 20401 acaaaccatt ccacgtcagg ttagtaatac aggtatatta attctatggc caatgctacc 20461 tacactattt aaccagcttg gtctacttga gaaaaagaaa tttatccatc gtcaggccca 20521 gtttaatgcc gttgattttc ttgattacct gatttgggga accgaagatg tgaaagtgga 20581 acgaaaggtt ttgaataatg ttctatgtgg gttaatggct gatgaaatta ctgaaccaat 20641 gcctattgaa ccagaaaaac aatggataat aattcaatgg ctggacgcta ttatctccca 20701 actttctggc tggaaaaagt taagtcgtaa tgacgtccgt caattatttc tacaacgacc 20761 aggagaatta ctgatcaatg aacaggaaat taaaatcaca atacagcaac aaccatttga 20821 tgctctgtta actgattggc cgtggccaat gaatatggct tgttttagct ggttgagtca 20881 accattaacc attacgtggt tataaccatt gaccacaatg acttagtctg agtaaaaaat 20941 atgaatatat cgcctgtttt ttatgattca ttgaatcagg ataacgaccg tgatctatcg 21001 tttttattta gcgaactgga acgaatagat ctcgctcttc aacaccattt ttattgtgta 21061 gaaagtcagc gaagtgagct cctggatgag tttctgctca ctgaggcgga agtggtgacc 21121 aggctggata agccacttgg taaacctcat tggataaatg atgattatct ggcgatatcg 21181 caaaagggca atgtaagcct aatggcagcg tccagattaa tggatctgat cgaacgcttt 21241 gaactgactg attttgagcg cgatgtttta ctattaggct tattgcccca ttttgatagc 21301 cgctattatc gactgttttc gctgattcaa gggggacaac agggtcgatt accttctttt 21361 gcgctggcat tggaactgtt ttgccactcg gcgctggaga aacaggtaca gcaagcgagt 21421 tttctgcacc gggcaccttt gatgggttgc cagctattat ccatcgatac tagtcaaaaa 21481 acgctggcct ggctccagac tccctttatt actgacagcg gggtatatca ctttttactg 21541 gggcatcact acattatgcc ggctttagaa cattgtgctg agtggttaac accgacaggg 21601 attggctgtt atcctgaagg attaaaacaa gtactgggta acgtattgtt atctgacaac 21661 gataatatta gaccgattgt cttattacgg ggaatggccg gcagtgccag agcttatacc 21721 attactaata tgatggcttc agaagggaag caaacactgc tggtagatat atccaaactt 21781 gctgatagcg atgaaaaaaa cattattctt cagataaagc atattttgcg ggaaacccgc 21841 atgcatggag catgtttatt attacggaat ttttgcttgt tagtggaaca gaataaacaa 21901 ctattggact ccctgtcaga gttattgaat caacctgaat taagaattgt ttgcctgatt 21961 gagccttatt ccccattggt atggctgaaa aagataccgg tattactgat tgagatgcca 22021 cttttaacgc ctgcggaaaa agccagattg ttaattgcca gcttaccgga taattgttcc 22081 gaggatattg atacgataac tttaagccag cgttacactt ttaacccaga aaccctgcca 22141 ttgattttgc aagaggccca gctttatcaa cagcagcgag atccgctgga tatcttgcag 22201 caatgcgata tacgccaggc attaaatttg cgtgctcaac aaaatttcgg tcaattggca 22261 cagcggatta ttcctaagcg ctcattaaag gatttattgg tatccgatga gattgctcag 22321 cagttacggg aaatactcat agcaattaag tatcgggaac aggttctggc gggagggttt 22381 aaagataaaa ttgcctatgg cactggtatc agcgccctgt tttatggtga ttcaggcact 22441 ggaaaaacca tggcagcaga agtgattgct gaccacattg gcgttgactt aataaaagtg 22501 gatttatcta cagtagtgaa taaatacatc ggtgaaacag aaaaaaactt atcccgtatt 22561 ttcgatttgg cggaacagga tgcaggggta ttattctttg atgaagctga cgcactgttt 22621 ggtaaacgca gtgaaactaa agattcccag gacagacatg ccaatattga agtttcttac 22681 ttattacagc gcctggagaa ttacccgggt ctggtcattt tatccaccaa taatcgtggt 22741 catttagaca gtgcttttaa tcgtcgtttt actttcatta cccgttttac ttacccggat 22801 gaaaaaatcc gtaaaaaaat gtggcaggaa atttggccta gaaatataaa aatatcggaa 22861 gatatcgatt ttaacgaatt agctcaacga acaagcgtga ctggcgcgaa tatccgcaat 22921 attgctttat tgtcttcatt ctttgcttca gagcagggga atgatgaagt cagtaatgaa 22981 aatattgaaa ttgcattgaa gcgtgaatta gctaaagtcg gacgattaac attttaaaag 23041 ttatcacaat gaaagtattg aaatattaaa taaatttatt accaaaaagt tatcacgata 23101 taatttaaga gaggtttttt atgttaaaca cgcaaactat tattgatgtc aataaggcaa 23161 tggatgccat gctgcgcgca tatctgaatc aagatattgc cattcgtttt gatctacctg 23221 aattggatac tatgcaatct gatgcgatgg taagtatctt tctttatgac attcatgaag 23281 atttacagct tcgctcggca gaatcaagag ggtttgatgt ttatgccggg aggttattgc 23341 ctggttgggt aaatattaaa tgtaactatc tgattaccta ttgggaagct tctaagccag 23401 cgactgatgc cagcagtccg gatagccaac ctgataacca ggcaatacaa gtgatgtcac 23461 aagtattaaa tgccttgatt aataatcgtc aattggcagg tattcctggt gcttatactc 23521 aggttgtacc gcctaaagag agtttaaata gcctggggaa tttctggcaa tcactgggta 23581 atcgcccacg gctttctctc aattattcag tgacagtacc tgttagccta aacgatggtc 23641 aggatagcgc gactccggtt accgcggttt cttctacagt ggaacaaacg gcatcgctca 23701 gtcaagaagt ggttagtcat gctttacgcg aattactcat tacggaatta ggaggaggag 23761 aggataaccg gttggtactg agtaaagttg aattatccgc agtgaaagag acgatgactc 23821 aagacagtcc ggctcagatg attatattgt tgtctgtttc aggcattaca cgacaggaat 23881 atttgaagga aattgataat atctttgatc gttgggtaaa taatgctgaa gttattacca 23941 ctattgatga ttgtgggatt agaattgaaa gtataacgaa agataatctt gtaggaattt 24001 aaattttttt aattaaataa aggaattaat attgatttga tagagcatgt aatcaataac 24061 ttctatgaaa atgttgaaaa acaatacacg gaaacactta acttacaatt aaattgttaa 24121 aggagatgat gataagaatg gtcagtcacc agataaaatg atctctggtc aattttagga 24181 aagctgaaaa aacctgacca aatgcgtcgt aaattctatg cttagaaaga ctcggtgcca 24241 gtgtaatacc taagatatgg tttttatttc catatctgtt cgaaaactta gaaataacaa 24301 gttcaacaat aaccagggac tttaaatatc ggatccttgt cgggaagatg cgtgatctga 24361 tccttcaact cagcaaaagt tcgatttatt caacaaagcc gccgtcccgt caagtcagcg 24421 taatgctctg ccagtgttac aaccaattaa ccaattctga ttagaaaaac tcatcgagca 24481 tcaaatgaaa ctgcaattta ttcatatcag gattatcaat accatatttt tgaaaaagcc 24541 gtttctgtaa tgaaggagaa aactcaccga ggcagttcca taggatggca agatcctggt 24601 atcggtctgc gattccgact cgtccaacat caatacaacc tattaatttc ccctcgtcaa 24661 aaataaggtt atcaagtgag aaatcaccat gagtgacgac tgaatccggt gagaatggca 24721 aaagcttatg catttctttc cagacttgtt caacaggcca gccattacgc tcgtcatcaa 24781 aatcactcgc atcaaccaaa ccgttattca ttcgtgattg cgcctgagcg agacgaaata 24841 cgcgatcgct gttaaaagga caattacaaa caggaatcga atgcaaccgg cgcaggaaca 24901 ctgccagcgc atcaacaata ttttcacctg aatcaggata ttcttctaat acctggaatg 24961 ctgttttccc ggggatcgca gtggtgagta accatgcatc atcaggagta cggataaaat 25021 gcttgatggt cggaagaggc ataaattccg tcagccagtt tagtctgacc atctcatctg 25081 taacatcatt ggcaacgcta cctttgccat gtttcagaaa caactctggc gcatcgggct 25141 tcccatacaa tcgatag //
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