| 质粒名称: | pCDH-METTL14-WT |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 141112 |
| 存储实验室: | Jianjun Chen |
|
载体骨架基本信息 |
|
| 载体名称: | pCDH |
| 载体出品公司: | -- |
| 空载体大小: | -- |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | 嘌呤霉素 |
| 载体抗性: | 氨苄霉素,100 μg/mL |
| 生长温度: | 37 ℃ |
| 宿主菌: | NEB Stable |
| 拷贝数: | -- |
|
插入基因信息 |
|
| 插入基因名称: | HMETTL14 |
| 物种来源: | H. sapiens (human) |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子: | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | -- |
| 5’克隆位点: | -- |
| 3’克隆位点: | -- |
| 5’测序引物 | -- |
| 3’测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pCDH-METTL14-WT was a gift from Jianjun Chen (Addgene plasmid # 141112 ; http://n2t.net/addgene:141112 ; RRID:Addgene_141112) |
| 参考文献部分: | METTL14 Inhibits Hematopoietic Stem/Progenitor Differentiation and Promotes Leukemogenesis via mRNA m(6)A Modification. Weng H, Huang H, Wu H, Qin X, Zhao BS, Dong L, Shi H, Skibbe J, Shen C, Hu C, Sheng Y, Wang Y, Wunderlich M, Zhang B, Dore LC, Su R, Deng X, Ferchen K, Li C, Sun M, Lu Z, Jiang X, Marcucci G, Mulloy JC, Yang J, Qian Z, Wei M, He C, Chen J. Cell Stem Cell. 2018 Feb 1;22(2):191-205.e9. doi: 10.1016/j.stem.2017.11.016. Epub 2017 Dec 28. 10.1016/j.stem.2017.11.016 PubMed 29290617 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pCDH-METTL14-WT 9590 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Overexpression of WT METTL14 in mammalian cells..
ACCESSION .
VERSION .
KEYWORDS pCDH-METTL14-WT
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9590)
AUTHORS Weng H, Huang H, Wu H, Qin X, Zhao BS, Dong L, Shi H, Skibbe J, Shen
C, Hu C, Sheng Y, Wang Y, Wunderlich M, Zhang B, Dore LC, Su R, Deng
X, Ferchen K, Li C, Sun M, Lu Z, Jiang X, Marcucci G, Mulloy JC,
Yang J, Qian Z, Wei M, He C, Chen J
TITLE METTL14 Inhibits Hematopoietic Stem/Progenitor Differentiation and
Promotes Leukemogenesis via mRNA m(6)A Modification.
JOURNAL Cell Stem Cell. 2018 Feb 1;22(2):191-205.e9. doi:
10.1016/j.stem.2017.11.016. Epub 2017 Dec 28.
PUBMED 29290617
REFERENCE 2 (bases 1 to 9590)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..9590
/organism="synthetic DNA construct"
/mol_type="other DNA"
misc_feature 168..284
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
promoter 374..577
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 528..548
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind 574..598
/label=LNCX
/note="Human CMV promoter, forward primer"
CDS 657..683
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
promoter 2093..2304
/label=EF-1-alpha core promoter
/note="core promoter for human elongation factor
EF-1-alpha"
LTR 2317..2585
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from human
T-cell leukemia virus (HTLV) type 1"
regulatory 2614..2623
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 2644..3306
/codon_start=1
/product="green fluorescent protein 2 from Pontellina
plumata, also known as ppluGFP2 (Shagin et al., 2004)"
/label=CopGFP
/translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
CDS 3376..3429
/codon_start=1
/product="2A peptide from Thosea asigna virus capsid
protein"
/label=T2A
/note="Eukaryotic ribosomes fail to insert a peptide bond
between the Gly and Pro residues, yielding separate
polypeptides."
/translation="EGRGSLLTCGDVEENPGP"
CDS 3430..4029
/codon_start=1
/gene="pac from Streptomyces alboniger"
/product="puromycin N-acetyltransferase"
/label=PuroR
/note="confers resistance to puromycin"
/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
primer_bind complement(3430..3449)
/label=Puro-R
/note="Puromycin resistance gene, reverse primer. Also
called puro-variant-R"
primer_bind 3926..3946
/label=Puro-F
/note="Puromycin resistance gene, forward primer"
misc_feature 4030..4618
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(4083..4103)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(4501..4512)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
LTR 4692..4925
/label=3' LTR (Delta-U3)
/note="self-inactivating 3' long terminal repeat (LTR) from
HIV-1"
polyA_signal 4997..5118
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(5034..5053)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 5088..5107
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
rep_origin 5158..5293
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 5220..5239
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
primer_bind complement(5326..5342)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(5326..5342)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(5339..5361)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 5350..5366
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(5374..5404)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 5419..5440
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(5557..5574)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(5728..6316)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(5808..5827)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(6487..7347)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 7110..7129
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(7348..7452)
/gene="bla"
/label=AmpR promoter
primer_bind 7520..7538
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
primer_bind complement(7576..7598)
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind 7698..7717
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 7911..7933
/label=M13/pUC Forward
/note="In lacZ gene"
primer_bind 7925..7942
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind 7926..7942
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
promoter 7957..8183
/label=RSV promoter
/note="Rous sarcoma virus enhancer/promoter"
LTR 8184..8364
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
misc_feature 8411..8536
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 9029..9262
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
CDS 9447..9491
/codon_start=1
/product="antigenic peptide corresponding to amino acids
655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
et al., 2013)"
/label=gp41 peptide
/note="recognized by the 2H10 single-chain llama nanobody"
/translation="KNEQELLELDKWASL"
ORIGIN
1 tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca
61 accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga
121 gacagatcca ttcgattagt gaacggatct cgacggtatc ggttaacttt taaaagaaaa
181 ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata
241 caaactaaag aattacaaaa acaaattaca aaattcaaaa ttttatcgat actagtatta
301 tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat
361 cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga
421 ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca
481 aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg
541 taggcgtgta cggtgggagg tttatataag cagagctcgt ttagtgaacc gtcagatcgc
601 ctggagacgc catccacgct gttttgacct ccatagaaga ttctagaacc accatgtacc
661 catacgatgt tccagattac gctgatagcc gcttgcagga gatccgggag cggcagaagt
721 tacggcgaca gctcctcgcg cagcagttgg gagctgaaag tgccgacagc attggtgccg
781 tgttaaatag caaagatgag cagagagaaa ttgctgaaac aagagaaact tgcagggctt
841 cctatgatac ctctgctcca aatgcaaaac gtaagtatct ggatgaagga gagacagatg
901 aagacaaaat ggaagaatat aaggatgaac tagaaatgca acaggatgaa gaaaatttgc
961 catatgaaga agagatttac aaagattcta gtacttttct taagggaaca cagagcttaa
1021 atccccataa tgattactgc caacattttg tagacactgg acatagacct cagaatttca
1081 tcagggatgt aggtttagct gacagatttg aagaatatcc taaactgagg gagctcatca
1141 ggctaaagga tgagttaata gctaaatcta acactcctcc catgtactta caagccgata
1201 tagaagcctt tgacatcaga gaactaacac ccaaatttga tgtgattctt ctggaacccc
1261 ctttagaaga atattacaga gaaactggca tcactgctaa tgaaaaatgc tggacttggg
1321 atgatattat gaagttagaa attgatgaga ttgcagcacc tcgatcattt atttttctct
1381 ggtgtggttc tggggagggg ttggaccttg gaagagtgtg tttacgaaaa tggggttaca
1441 gaagatgtga agatatttgt tggattaaaa ccaataaaaa caatcctggg aagactaaga
1501 ctttagatcc aaaggctgtc tttcagagaa caaaggaaca ctgcctcatg gggatcaaag
1561 gaactgtgaa gcgtagcaca gacggggact tcattcatgc taatgttgac attgacttaa
1621 ttatcacaga agaacctgaa attggcaata tagaaaaacc tgtagaaatt tttcatataa
1681 ttgagcattt ttgtcttggt agaagacgcc ttcatctatt tggaagagat agtacaattc
1741 gaccaggctg gctcacagtt ggaccaacgc ttacaaatag caactacaat gcagaaacat
1801 atgcatccta tttcagtgct cctaattcct acttgactgg ttgtacagaa gaaattgaga
1861 gacttcgacc aaaatcgcct cctcccaaat ctaaatctga ccgaggaggt ggagctccca
1921 gaggtggagg aagaggtgga acttctgctg gccgtggacg agaaagaaat agatctaact
1981 tccgaggaga aagaggtggc tttagagggg gccgtggagg agcacacaga ggtggctttc
2041 cacctcgata agcggccgcg aaggatctgc gatcgctccg gtgcccgtca gtgggcagag
2101 cgcacatcgc ccacagtccc cgagaagttg gggggagggg tcggcaattg aacgggtgcc
2161 tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg tgtactggct ccgccttttt
2221 cccgagggtg ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt tctttttcgc
2281 aacgggtttg ccgccagaac acagctgaag cttcgagggg ctcgcatctc tccttcacgc
2341 gcccgccgcc ctacctgagg ccgccatcca cgccggttga gtcgcgttct gccgcctccc
2401 gcctgtggtg cctcctgaac tgcgtccgcc gtctaggtaa gtttaaagct caggtcgaga
2461 ccgggccttt gtccggcgct cccttggagc ctacctagac tcagccggct ctccacgctt
2521 tgcctgaccc tgcttgctca actctacgtc tttgtttcgt tttctgttct gcgccgttac
2581 agatccaagc tgtgaccggc gcctacgcta gacgccacca tggagagcga cgagagcggc
2641 ctgcccgcca tggagatcga gtgccgcatc accggcaccc tgaacggcgt ggagttcgag
2701 ctggtgggcg gcggagaggg cacccccaag cagggccgca tgaccaacaa gatgaagagc
2761 accaaaggcg ccctgacctt cagcccctac ctgctgagcc acgtgatggg ctacggcttc
2821 taccacttcg gcacctaccc cagcggctac gagaacccct tcctgcacgc catcaacaac
2881 ggcggctaca ccaacacccg catcgagaag tacgaggacg gcggcgtgct gcacgtgagc
2941 ttcagctacc gctacgaggc cggccgcgtg atcggcgact tcaaggtggt gggcaccggc
3001 ttccccgagg acagcgtgat cttcaccgac aagatcatcc gcagcaacgc caccgtggag
3061 cacctgcacc ccatgggcga taacgtgctg gtgggcagct tcgcccgcac cttcagcctg
3121 cgcgacggcg gctactacag cttcgtggtg gacagccaca tgcacttcaa gagcgccatc
3181 caccccagca tcctgcagaa cgggggcccc atgttcgcct tccgccgcgt ggaggagctg
3241 cacagcaaca ccgagctggg catcgtggag taccagcacg ccttcaagac ccccatcgcc
3301 ttcgccagat cccgcgctca gtcgtccaat tctgccgtgg acggcaccgc cggacccggc
3361 tccaccggat ctcgcgaggg cagaggaagt cttctaacat gcggtgacgt ggaggagaat
3421 cccggcccta tgaccgagta caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc
3481 agggccgtac gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc
3541 gatccggacc gccacatcga gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc
3601 gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc
3661 acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg catggccgag
3721 ttgagcggtt cccggctggc cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg
3781 cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca ccagggcaag
3841 ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc
3901 gccttcctgg agacctccgc gccccgcaac ctccccttct acgagcggct cggcttcacc
3961 gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc
4021 ggtgcctgaa atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac
4081 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt
4141 gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat
4201 gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca
4261 acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc
4321 cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg
4381 gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct
4441 tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct
4501 tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt
4561 ccgcgtctcc gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcct
4621 ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga
4681 aaagggggga ctggaagggc taattcactc ccaacgaaga taagatctgc tttttgcttg
4741 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
4801 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
4861 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
4921 tagcagtagt agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat
4981 atcagagagt gagaggaact tgtttattgc agcttataat ggttacaaat aaagcaatag
5041 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
5101 actcatcaat gtatcttatc atgtctggct ctagctatcc cgcccctaac tccgcccatc
5161 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
5221 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc
5281 ttttttggag gcctagactt ttgcagagac caaattcgta atcatgtcat agctgtttcc
5341 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
5401 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
5461 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
5521 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
5581 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
5641 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
5701 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
5761 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
5821 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
5881 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
5941 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
6001 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
6061 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
6121 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
6181 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
6241 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
6301 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
6361 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
6421 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
6481 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
6541 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
6601 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
6661 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
6721 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
6781 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
6841 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
6901 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
6961 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
7021 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
7081 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
7141 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
7201 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
7261 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
7321 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
7381 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
7441 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat
7501 catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg
7561 tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta
7621 agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg
7681 gggctggctt aactatgcgg catcagagca gattgtactg agagtgcacc atatgcggtg
7741 tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgccatt cgccattcag
7801 gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc
7861 gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg
7921 acgttgtaaa acgacggcca gtgccaagct gacgcgtgta gtcttatgca atactcttgt
7981 agtcttgcaa catggtaacg atgagttagc aacatgcctt acaaggagag aaaaagcacc
8041 gtgcatgccg attggtggaa gtaaggtggt acgatcgtgc cttattagga aggcaacaga
8101 cgggtctgac atggattgga cgaaccactg aattgccgca ttgcagagat attgtattta
8161 agtgcctagc tcgatacata aacgggtctc tctggttaga ccagatctga gcctgggagc
8221 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
8281 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
8341 agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag cgaaagggaa
8401 accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga
8461 ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga aggagagaga
8521 tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg
8581 gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg caagcaggga
8641 gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct gtagacaaat
8701 actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat cattatataa
8761 tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca ccaaggaagc
8821 tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc aagcggccac
8881 tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat
8941 ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg
9001 tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag
9061 caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt
9121 ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt
9181 tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat
9241 acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca
9301 ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca
9361 cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa
9421 ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat
9481 gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata aaattattca
9541 taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt
//
