| 质粒名称: | pCE-mp53DD |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 41856 |
| 存储实验室: | Shinya Yamanaka |
|
载体骨架基本信息 |
|
| 载体名称: | pCE |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 9364 bp |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | Trp53,p53,Tp53 |
| 物种来源: | M. musculus (mouse) |
| 插入大小: | 334 bp |
| 基因突变: | Δ40-903 |
| GenBank编号 | NM_011640 |
| 启动子 | CAG |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | EcoRI (未破坏) |
| 3' 克隆位点: | EcoRI (未破坏) |
| 5' 测序引物 | pCAG-F |
| 3' 测序引物 | TTAGCCAGAAGTCAGATGCTC |
|
文献引用方法 |
|
| 材料和方法部分: | pCE-mp53DD was a gift from Shinya Yamanaka (Addgene plasmid # 41856 ; http://n2t.net/addgene:41856 ; RRID:Addgene_41856) |
| 参考文献部分: | An Efficient Non-viral Method to Generate Integration-Free Human iPS Cells from Cord Blood and Peripheral Blood Cells. Okita K, Yamakawa T, Matsumura Y, Sato Y, Amano N, Watanabe A, Goshima N, Yamanaka S. Stem Cells. 2012 Nov 29. doi: 10.1002/stem.1293. 10.1002/stem.1293 PubMed 23193063 |
买家导航
pCEmp53DD载体基本信息
pCEmp53DD载体图谱质粒图谱和多克隆位点信息
pCEmp53DD载体简介
pCEmp53DD载体序列
LOCUS pCE-mp53DD 9690 bp ds-DNA circular SYN 08-FEB-2019
DEFINITION Non-integrating (episomal) expression of mouse p53DD - p53
carboxy-terminal dominant-negative fragment .
ACCESSION .
VERSION .
KEYWORDS pCE-mp53DD
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9690)
AUTHORS Okita K, Yamakawa T, Matsumura Y, Sato Y, Amano N, Watanabe A,
Goshima N, Yamanaka S
TITLE An Efficient Non-viral Method to Generate Integration-Free Human iPS
Cells from Cord Blood and Peripheral Blood Cells.
JOURNAL Stem Cells. 2012 Nov 29. doi: 10.1002/stem.1293.
PUBMED 23193063
REFERENCE 2 (bases 1 to 9690)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Feb 8, 2019 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..9690
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 4..383
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
intron 662..1678
/label=chimeric intron
/note="chimera between introns from chicken beta-actin and
rabbit beta-globin"
primer_bind 1602..1624
/label=pCAGGS-5
/note="Chimeric intron in CAG promoter, forward primer"
primer_bind 1686..1705
/label=pCAG-F
/note="Rabbit beta-globin intron, for pCAG plasmids,
forward primer"
primer_bind complement(2143..2162)
/label=Bglob-pA-R
/note="Rabbit beta-globin polyA region, reverse primer"
polyA_site 2208..2263
/label=-beta-globin poly(A) signal
/note="rabbit beta-globin polyadenylation signal"
primer_bind complement(2262..2281)
/label=rbglobpA-R
/note="Rabbit beta-globin polyA, reverse primer. Also
called rb-glob-pA-term-R"
primer_bind complement(2624..2640)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(2624..2640)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(2637..2659)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 2648..2664
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(2672..2702)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 2717..2738
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
CDS 3217..5136
/codon_start=1
/product="Epstein-Barr nuclear antigen 1, also known as
EBNA-1"
/label=EBNA1
/note="crucial for latent viral infection, and for episomal
amplification of vectors containing the oriP origin (Okita
et al., 2013)"
/translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR
GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG
AGGGAGAGGGAGGAGGAGGAG?GGG?GAGGGAGG?GG?G?GGGAG?GGGAGGAGAGGGA
GGGGG?GAGGGAGAGGGAGGAGAGGAGGAGGAGGGGGAG?GGGGGAGGAGAGGAGAGGG
AGGAGGAGAGGAGAGGAGAGGAGGGGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGG
AGGAGAGGGGG?G?GG?GG?GAGGAGGAGAGGGAGAGGAGAGGGGRGRGGSGGRGRGGS
GGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPPPG
RRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGRRK
KGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSLYN
LRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLKDA
IKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDEGE
EGQE"
rep_origin 5438..7227
/label=oriP
/note="Epstein-Barr virus oriP replication origin (Yates et
al., 2000)"
primer_bind complement(7768..7785)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(7939..8527)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(8019..8038)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(8698..9558)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 9321..9340
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(9559..9663)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 gtcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
181 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
361 tattagtcat cgctattacc atggtcgagg tgagccccac gttctgcttc actctcccca
421 tctccccccc ctccccaccc ccaattttgt atttatttat tttttaatta ttttgtgcag
481 cgatgggggc gggggggggg ggggggcgcg cgccaggcgg ggcggggcgg ggsggggggg
541 ggggssggbg kskcskrsrs gygcggcggc agccaatcag agcggcgcgc tccgaaagtt
601 tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc
661 gggagtcgct gcgcgctgcc ttcgccccgt gccccgctcc gccgccgcct cgcgccgccc
721 gccccggctc tgactgaccg cgttactccc acaggtgagc gggcgggacg gcccttctcc
781 tccgggctgt aattagcgct tggtttaatg acggcttgtt tcttttctgt ggctgcgtga
841 aagccttgag gggctccggg agggcccttt gtgcgggggg agcggctcgg ggggtgcgtg
901 cgtgtgtgtg tgcgtgggga gcgccgcgtg cggctccgcg ctgcccggcg gctgtgagcg
961 ctgcgggcgc ggcgcggggc tttgtgcgct ccgcagtgtg cgcgagggga gcgcggccgg
1021 gggcggtgcc ccgcggtgcg gggggggctg cgaggggaac aaaggctgcg tgcggggtgt
1081 gtgcgtgggg gggtgagcag ggggtgtggg cgcgtcggtc gggctgcaac cccccctgca
1141 cccccctccc cgagttgctg agcacggccc ggcttcgggt gcggggctcc gtacggggcg
1201 tggcgcgggg ctcgccgtgc cgggcggggg gtggcggcag gtgggggtgc cgggcggggc
1261 ggggccgcct cgggccgggg agggctcggg ggaggggcgc ggcggccccc ggagcgccgg
1321 cggctgtcga ggcgcggcga gccgcagcca ttgcctttta tggtaatcgt gcgagagggc
1381 gcagggactt cctttgtccc aaatctgtgc ggagccgaaa tctgggaggc gccgccgcac
1441 cccctctagc gggcgcgggg cgaagcggtg cggcgccggc aggaaggaaa tgggcgggga
1501 gggccttcgt gcgtcgccgc gccgccgtcc ccttctccct ctccagcctc ggggctgtcc
1561 gcggggggac ggctgccttc gggggggacg gggcagggcg gggttcggct tctggcgtgt
1621 gaccggcggc tctagagcct ctgctaacca tgttcatgcc ttcttctttt tcctacagct
1681 cctgggcaac gtgctggtta ttgtgctgtc tcatcatttt ggcaaagaat tcgcccttca
1741 ccaggatgac tgccatggag gagtcacagt cggatatcag cctcaagaga gcgctgccca
1801 cctgcacaag cgcctctccc ccgcaaaaga aaaaaccact tgatggagag tatttcaccc
1861 tcaagatccg cgggcgtaaa cgcttcgaga tgttccggga gctgaatgag gccttagagt
1921 taaaggatgc ccatgctaca gaggagtctg gagacagcag ggctcactcc agctacctga
1981 agaccaagaa gggccagtct acttcccgcc ataaaaaaac aatggtcaag aaagtggggc
2041 ctgactcaga ctgaagggcg aattcactcc tcaggtgcag gctgcctatc agaaggtggt
2101 ggctggtgtg gccaatgccc tggctcacaa ataccactga gatctttttc cctctgccaa
2161 aaattatggg gacatcatga agccccttga gcatctgact tctggctaat aaaggaaatt
2221 tattttcatt gcaatagtgt gttggaattt tttgtgtctc tcactcggaa ggacatatgg
2281 gagggcaaat catttaaaac atcagaatga gtatttggtt tagagtttgg caacatatgc
2341 ccatatgctg gctgccatga acaaaggttg gctataaaga ggtcatcagt atatgaaaca
2401 gccccctgct gtccattcct tattccatag aaaagccttg acttgaggtt agattttttt
2461 tatattttgt tttgtgttat ttttttcttt aacatcccta aaattttcct tacatgtttt
2521 actagccaga tttttcctcc tctcctgact actcccagtc atagctgtcc ctcttctctt
2581 atggagatcc ctcgacctgc agcccaagct tggcgtaatc atggtcatag ctgtttcctg
2641 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
2701 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
2761 ctttccagtc gggaaacctg tcgtgccagc ggatctcaat tctcatgttt gacagcttat
2821 catcgataag ctgatcctca caggccgcac ccagcttttc ttccgttgcc ccagtagcat
2881 ctctgtctgg tgaccttgaa gaggaagagg aggggtcccg agaatcccca tccctaccgt
2941 ccagcaaaaa gggggacgag gaatttgagg cctggcttga ggctcaggac gcaaatcttg
3001 aggatgttca gcgggagttt tccgggctgc gagtaattgg tgatgaggac gaggatggtt
3061 cggaggatgg ggaattttca gacctggatc tgtctgacag cgaccatgaa ggggatgagg
3121 gtgggggggc tgttggaggg ggcaggagtc tgcactccct gtattcactg agcgtcgtct
3181 aataaagatg tctattgatc tcttttagtg tgaatcatgt ctgacgaggg gccaggtaca
3241 ggacctggaa atggcctagg agagaaggga gacacatctg gaccagaagg ctccggcggc
3301 agtggacctc aaagaagagg gggtgataac catggacgag gacggggaag aggacgagga
3361 cgaggaggcg gaagaccagg agccccgggc ggctcaggat cagggccaag acatagagat
3421 ggtgtccgga gaccccaaaa acgtccaagt tgcattggct gcaaagggac ccacggtgga
3481 acaggagcag gagcaggagc gggaggggca ggagcaggag gggcaggagc aggaggaggg
3541 gcaggagcag gaggaggggc aggaggggca ggaggggcag gaggggcagg agsaggagga
3601 ggggsaggag caggaggagg ggcaggaggg gsaggagggg saggagsagg aggaggggca
3661 ggagsaggag gaggggcagg aggggcagga gcaggaggag gggcaggagg gggaggaggg
3721 gsaggagcag gaggaggggc aggagcagga ggaggggcag gaggggcagg agcaggaggg
3781 gcaggagggg caggaggggc aggaggagga ggaggggcag gagsaggagg gggaggaggg
3841 gcaggagggg caggagcagg aggggcagga gcaggaggag gggcaggagg ggcaggaggg
3901 gcaggagcag gaggggcagg agcaggaggg gcaggagcag gaggggcagg aggrggaggr
3961 gcaggagggg caggagcagg aggggcagga ggggcaggag caggaggggc aggaggggca
4021 ggagcaggag gggcaggagg ggcaggagca ggaggagggg caggaggggc aggagcagga
4081 gggggaggag gggsaggags aggaggggsa ggaggggsag gagcaggagg ggcaggaggg
4141 gcaggagcag gaggaggggc aggagcagga ggggcaggag caggaggtgg aggccggggt
4201 cgaggaggca gtggaggccg gggtcgagga ggtagtggag gccggggtcg aggaggtagt
4261 ggaggccgcc ggggtagagg acgtgaaaga gccagggggg gaagtcgtga aagagccagg
4321 gggagaggtc gtggacgtgg agaaaagagg cccaggagtc ccagtagtca gtcatcatca
4381 tccgggtctc caccgcgcag gccccctcca ggtagaaggc catttttcca ccctgtaggg
4441 gaagccgatt attttgaata ccaccaagaa ggtggcccag atggtgagcc tgacgtgccc
4501 ccgggagcga tagagcaggg ccccgcagat gacccaggag aaggcccaag cactggaccc
4561 cggggtcagg gtgatggagg caggcgcaaa aaaggagggt ggtttggaaa gcatcgtggt
4621 caaggaggtt ccaacccgaa atttgagaac attgcagaag gtttaagagc tctcctggct
4681 aggagtcacg tagaaaggac taccgacgaa ggaacttggg tcgccggtgt gttcgtatat
4741 ggaggtagta agacctccct ttacaaccta aggcgaggaa ctgcccttgc tattccacaa
4801 tgtcgtctta caccattgag tcgtctcccc tttggaatgg cccctggacc cggcccacaa
4861 cctggcccgc taagggagtc cattgtctgt tatttcatgg tctttttaca aactcatata
4921 tttgctgagg ttttgaagga tgcgattaag gaccttgtta tgacaaagcc cgctcctacc
4981 tgcaatatca gggtgactgt gtgcagcttt gacgatggag tagatttgcc tccctggttt
5041 ccacctatgg tggaaggggc tgccgcggag ggtgatgacg gagatgacgg agatgaagga
5101 ggtgatggag atgagggtga ggaagggcag gagtgatgta acttgttagg agacgccctc
5161 aatcgtatta aaagccgtgt attcccccgc actaaagaat aaatccccag tagacatcat
5221 gcgtgctgtt ggtgtatttc tggccatctg tcttgtcacc attttcgtcc tcccaacatg
5281 gggcaattgg gcatacccat gttgtcacgt cactcagctc cgcgctcaac accttctcgc
5341 gttggaaaac attagcgaca tttacctggt gagcaatcag acatgcgacg gctttagcct
5401 ggcctcctta aattcaccta agaatgggag caaccagcag gaaaaggaca agcagcgaaa
5461 attcacgccc ccttgggagg tggcggcata tgcaaaggat agcactccca ctctactact
5521 gggtatcata tgctgactgt atatgcatga ggatagcata tgctacccgg atacagatta
5581 ggatagcata tactacccag atatagatta ggatagcata tgctacccag atatagatta
5641 ggatagccta tgctacccag atataaatta ggatagcata tactacccag atatagatta
5701 ggatagcata tgctacccag atatagatta ggatagccta tgctacccag atatagatta
5761 ggatagcata tgctacccag atatagatta ggatagcata tgctatccag atatttgggt
5821 agtatatgct acccagatat aaattaggat agcatatact accctaatct ctattaggat
5881 agcatatgct acccggatac agattaggat agcatatact acccagatat agattaggat
5941 agcatatgct acccagatat agattaggat agcctatgct acccagatat aaattaggat
6001 agcatatact acccagatat agattaggat agcatatgct acccagatat agattaggat
6061 agcctatgct acccagatat agattaggat agcatatgct atccagatat ttgggtagta
6121 tatgctaccc atggcaacat tagcccaccg tgctctcagc gacctcgtga atatgaggac
6181 caacaaccct gtgcttggcg ctcaggcgca agtgtgtgta atttgtcctc cagatcgcag
6241 caatcgcgcc cctatcttgg cccgcccacc tacttatgca ggtattcccc ggggtgccat
6301 tagtggtttt gtgggcaagt ggtttgaccg cagtggttag cggggttaca atcagccaag
6361 ttattacacc cttattttac agtccaaaac cgcagggcgg cgtgtggggg ctgacgcgtg
6421 cccccactcc acaatttcaa aaaaaagagt ggccacttgt ctttgtttat gggccccatt
6481 ggcgtggagc cccgtttaat tttcgggggt gttagagaca accagtggag tccgctgctg
6541 tcggcgtcca ctctctttcc ccttgttaca aatagagtgt aacaacatgg ttcacctgtc
6601 ttggtccctg cctgggacac atcttaataa ccccagtatc atattgcact aggattatgt
6661 gttgcccata gccataaatt cgtgtgagat ggacatccag tctttacggc ttgtccccac
6721 cccatggatt tctattgtta aagatattca gaatgtttca ttcctacact agtatttatt
6781 gcccaagggg tttgtgaggg ttatattggt gtcatagcac aatgccacca ctgaaccccc
6841 cgtccaaatt ttattctggg ggcgtcacct gaaaccttgt tttcgagcac ctcacataca
6901 ccttactgtt cacaactcag cagttattct attagctaaa cgaaggagaa tgaagaagca
6961 ggcgaagatt caggagagtt cactgcccgc tccttgatct tcagccactg cccttgtgac
7021 taaaatggtt cactaccctc gtggaatcct gaccccatgt aaataaaacc gtgacagctc
7081 atggggtggg agatatcgct gttccttagg acccttttac taaccctaat tcgatagcat
7141 atgcttcccg ttgggtaaca tatgctattg aattagggtt agtctggata gtatatacta
7201 ctacccggga agcatatgct acccgtttag ggttaacaag ggggccttat aaacactatt
7261 gctaatgccc tcttgagggt ccgcttatcg gtagctacac aggcccctct gattgacgtt
7321 ggtgtagcct cccgtagtct tcctgggccc ctgggaggta catgtccccc agcattggtg
7381 taagagcttc agccaagagt tacacataaa ggcaatgttg tgttgcagtc cacagactgc
7441 aaagtctgct ccaggatgaa agccactcag tgttggcaaa tgtgcacatc catttataag
7501 gatgtcaact acagtcagag aacccctttg tgtttggtcc ccccccgtgt cacatgtgga
7561 acagggccca gttggcaagt tgtaccaacc aactgaaggg attacatgca ctgccccgcg
7621 aagaaggggc agagatgtcg tagtcaggtt tagttcgtcc ggggcgggga tcgatcctct
7681 agagtcgacg ctagcggatc cgctgcatta atgaatcggc caacgcgcgg ggagaggcgg
7741 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
7801 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
7861 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
7921 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
7981 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
8041 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
8101 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
8161 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
8221 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
8281 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
8341 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
8401 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
8461 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
8521 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
8581 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
8641 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
8701 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
8761 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
8821 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
8881 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
8941 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
9001 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
9061 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
9121 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
9181 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
9241 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
9301 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
9361 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
9421 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
9481 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
9541 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
9601 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
9661 gcgcacattt ccccgaaaag tgccacctgg
//
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pCEmp53DD载体质粒应用举例
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