| 质粒名称: | pCMV-ABE7.10 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 102919 |
| 存储实验室: | David Liu |
|
载体骨架基本信息 |
|
| 载体名称: | pBR322/CMV |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 3388 bp |
| 完整质粒大小: | 8727bp |
| 载体修饰: | 人细胞色素c氧化酶亚基VIII（human cytochrome C oxidase subunit VIII） |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | 新霉素（G418） |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | ABE7.10 |
| 物种来源: | -- |
| 插入大小: | 5328 bp |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | 人细胞色素c氧化酶亚基VIII（N端） |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | AgeI(未破坏) |
| 3' 克隆位点: | NotI (未破坏) |
| 5' 测序引物 | CMV-F |
| 3' 测序引物 | SV40pA-R |
|
文献引用方法 |
|
| 材料和方法部分: | pCMV-ABE7.10 was a gift from David Liu (Addgene plasmid # 102919 ; http://n2t.net/addgene:102919 ; RRID:Addgene_102919) |
| 参考文献部分: |
Programmable base editing of A*T to G*C in genomic DNA without DNA cleavage. Gaudelli NM, Komor AC, Rees HA, Packer MS, Badran AH, Bryson DI, Liu DR. Nature. 2017 Nov 23;551(7681):464-471. doi: 10.1038/nature24644. Epub 2017 Oct 25. 10.1038/nature24644 PubMed 29160308
Related items:
From this article
David Liu Lab Plasmids
CRISPR/Cas Items
Commonly requested with:
BE4-Gam
pCMV-ABE7.9
pCMV-BE3
pCMV-ABE6.3
BE4
|
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pCMV-ABE7.10 8727 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION expresses ABE7.10 in mammalian cells.
ACCESSION .
VERSION .
KEYWORDS pCMV-ABE7.10
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8727)
AUTHORS Gaudelli NM, Komor AC, Rees HA, Packer MS, Badran AH, Bryson DI, Liu
DR
TITLE Programmable base editing of A*T to G*C in genomic DNA without DNA
cleavage.
JOURNAL Nature. 2017 Nov 23;551(7681):464-471. doi: 10.1038/nature24644.
Epub 2017 Oct 25.
PUBMED 29160308
REFERENCE 2 (bases 1 to 8727)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..8727
/organism="synthetic DNA construct"
/mol_type="other DNA"
CDS 1098..5198
/codon_start=1
/product="nickase mutant of the Cas9 endonuclease from the
Streptococcus pyogenes Type II CRISPR/Cas system"
/label=Cas9(D10A)
/note="generates RNA-guided single strand nicks in DNA due
to the D10A mutation in the RuvC catalytic domain"
/translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
ATLIHQSITGLYETRIDLSQLGGD"
CDS 5211..5231
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS 5240..5257
/codon_start=1
/product="6xHis affinity tag"
/label=6xHis
/translation="HHHHHH"
primer_bind complement(5280..5297)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 5286..5510
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
primer_bind complement(5581..5597)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(5581..5597)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(5594..5616)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 5605..5621
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(5629..5659)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 5674..5695
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(5812..5829)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(5983..6571)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(6063..6082)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(6742..7602)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 7365..7384
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(7603..7707)
/gene="bla"
/label=AmpR promoter
primer_bind complement(7786..7805)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
enhancer 7977..8356
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 8357..8560
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 8511..8531
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind 8602..8621
/label=T7
/note="T7 promoter, forward primer"
promoter 8602..8620
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
1 cagtactcgt gcataacaat cgcgtaatcg gcgaaggttg gaataggccg atcggacgcc
61 acgaccccac tgcacatgcg gaaatcatgg cccttcgaca gggagggctt gtgatgcaga
121 attatcgact tatcgatgcg acgctgtacg tcacgcttga accttgcgta atgtgcgcgg
181 gagctatgat tcactcccgc attggacgag ttgtattcgg tgcccgcgac gccaagacgg
241 gtgccgcagg ttcactgatg gacgtgctgc atcacccagg catgaaccac cgggtagaaa
301 tcacagaagg catattggcg gacgaatgtg cggcgctgtt gtccgacttt tttcgcatgc
361 ggaggcagga gatcaaggcc cagaaaaaag cacaatcctc tactgactct ggtggttctt
421 ctggtggttc tagcggcagc gagactcccg ggacctcaga gtccgccaca cccgaaagtt
481 ctggtggttc ttctggtggt tcttccgaag tcgagttttc ccatgagtac tggatgagac
541 acgcattgac tctcgcaaag agggctcgag atgaacgcga ggtgcccgtg ggggcagtac
601 tcgtgctcaa caatcgcgta atcggcgaag gttggaatag ggcaatcgga ctccacgacc
661 ccactgcaca tgcggaaatc atggcccttc gacagggagg gcttgtgatg cagaattatc
721 gacttatcga tgcgacgctg tacgtcacgt ttgaaccttg cgtaatgtgc gcgggagcta
781 tgattcactc ccgcattgga cgagttgtat tcggtgttcg caacgccaag acgggtgccg
841 caggttcact gatggacgtg ctgcattacc caggcatgaa ccaccgggta gaaatcacag
901 aaggcatatt ggcggacgaa tgtgcggcgc tgttgtgtta cttttttcgc atgcccaggc
961 aggtctttaa cgcccagaaa aaagcacaat cctctactga ctctggtggt tcttctggtg
1021 gttctagcgg cagcgagact cccgggacct cagagtccgc cacacccgaa agttctggtg
1081 gttcttctgg tggttctgat aaaaagtatt ctattggttt agccatcggc actaattccg
1141 ttggatgggc tgtcataacc gatgaataca aagtaccttc aaagaaattt aaggtgttgg
1201 ggaacacaga ccgtcattcg attaaaaaga atcttatcgg tgccctccta ttcgatagtg
1261 gcgaaacggc agaggcgact cgcctgaaac gaaccgctcg gagaaggtat acacgtcgca
1321 agaaccgaat atgttactta caagaaattt ttagcaatga gatggccaaa gttgacgatt
1381 ctttctttca ccgtttggaa gagtccttcc ttgtcgaaga ggacaagaaa catgaacggc
1441 accccatctt tggaaacata gtagatgagg tggcatatca tgaaaagtac ccaacgattt
1501 atcacctcag aaaaaagcta gttgactcaa ctgataaagc ggacctgagg ttaatctact
1561 tggctcttgc ccatatgata aagttccgtg ggcactttct cattgagggt gatctaaatc
1621 cggacaactc ggatgtcgac aaactgttca tccagttagt acaaacctat aatcagttgt
1681 ttgaagagaa ccctataaat gcaagtggcg tggatgcgaa ggctattctt agcgcccgcc
1741 tctctaaatc ccgacggcta gaaaacctga tcgcacaatt acccggagag aagaaaaatg
1801 ggttgttcgg taaccttata gcgctctcac taggcctgac accaaatttt aagtcgaact
1861 tcgacttagc tgaagatgcc aaattgcagc ttagtaagga cacgtacgat gacgatctcg
1921 acaatctact ggcacaaatt ggagatcagt atgcggactt atttttggct gccaaaaacc
1981 ttagcgatgc aatcctccta tctgacatac tgagagttaa tactgagatt accaaggcgc
2041 cgttatccgc ttcaatgatc aaaaggtacg atgaacatca ccaagacttg acacttctca
2101 aggccctagt ccgtcagcaa ctgcctgaga aatataagga aatattcttt gatcagtcga
2161 aaaacgggta cgcaggttat attgacggcg gagcgagtca agaggaattc tacaagttta
2221 tcaaacccat attagagaag atggatggga cggaagagtt gcttgtaaaa ctcaatcgcg
2281 aagatctact gcgaaagcag cggactttcg acaacggtag cattccacat caaatccact
2341 taggcgaatt gcatgctata cttagaaggc aggaggattt ttatccgttc ctcaaagaca
2401 atcgtgaaaa gattgagaaa atcctaacct ttcgcatacc ttactatgtg ggacccctgg
2461 cccgagggaa ctctcggttc gcatggatga caagaaagtc cgaagaaacg attactccat
2521 ggaattttga ggaagttgtc gataaaggtg cgtcagctca atcgttcatc gagaggatga
2581 ccaactttga caagaattta ccgaacgaaa aagtattgcc taagcacagt ttactttacg
2641 agtatttcac agtgtacaat gaactcacga aagttaagta tgtcactgag ggcatgcgta
2701 aacccgcctt tctaagcgga gaacagaaga aagcaatagt agatctgtta ttcaagacca
2761 accgcaaagt gacagttaag caattgaaag aggactactt taagaaaatt gaatgcttcg
2821 attctgtcga gatctccggg gtagaagatc gatttaatgc gtcacttggt acgtatcatg
2881 acctcctaaa gataattaaa gataaggact tcctggataa cgaagagaat gaagatatct
2941 tagaagatat agtgttgact cttaccctct ttgaagatcg ggaaatgatt gaggaaagac
3001 taaaaacata cgctcacctg ttcgacgata aggttatgaa acagttaaag aggcgtcgct
3061 atacgggctg gggacgattg tcgcggaaac ttatcaacgg gataagagac aagcaaagtg
3121 gtaaaactat tctcgatttt ctaaagagcg acggcttcgc caataggaac tttatgcagc
3181 tgatccatga tgactcttta accttcaaag aggatataca aaaggcacag gtttccggac
3241 aaggggactc attgcacgaa catattgcga atcttgctgg ttcgccagcc atcaaaaagg
3301 gcatactcca gacagtcaaa gtagtggatg agctagttaa ggtcatggga cgtcacaaac
3361 cggaaaacat tgtaatcgag atggcacgcg aaaatcaaac gactcagaag gggcaaaaaa
3421 acagtcgaga gcggatgaag agaatagaag agggtattaa agaactgggc agccagatct
3481 taaaggagca tcctgtggaa aatacccaat tgcagaacga gaaactttac ctctattacc
3541 tacaaaatgg aagggacatg tatgttgatc aggaactgga cataaaccgt ttatctgatt
3601 acgacgtcga tcacattgta ccccaatcct ttttgaagga cgattcaatc gacaataaag
3661 tgcttacacg ctcggataag aaccgaggga aaagtgacaa tgttccaagc gaggaagtcg
3721 taaagaaaat gaagaactat tggcggcagc tcctaaatgc gaaactgata acgcaaagaa
3781 agttcgataa cttaactaaa gctgagaggg gtggcttgtc tgaacttgac aaggccggat
3841 ttattaaacg tcagctcgtg gaaacccgcc aaatcacaaa gcatgttgca cagatactag
3901 attcccgaat gaatacgaaa tacgacgaga acgataagct gattcgggaa gtcaaagtaa
3961 tcactttaaa gtcaaaattg gtgtcggact tcagaaagga ttttcaattc tataaagtta
4021 gggagataaa taactaccac catgcgcacg acgcttatct taatgccgtc gtagggaccg
4081 cactcattaa gaaatacccg aagctagaaa gtgagtttgt gtatggtgat tacaaagttt
4141 atgacgtccg taagatgatc gcgaaaagcg aacaggagat aggcaaggct acagccaaat
4201 acttctttta ttctaacatt atgaatttct ttaagacgga aatcactctg gcaaacggag
4261 agatacgcaa acgaccttta attgaaacca atggggagac aggtgaaatc gtatgggata
4321 agggccggga cttcgcgacg gtgagaaaag ttttgtccat gccccaagtc aacatagtaa
4381 agaaaactga ggtgcagacc ggagggtttt caaaggaatc gattcttcca aaaaggaata
4441 gtgataagct catcgctcgt aaaaaggact gggacccgaa aaagtacggt ggcttcgata
4501 gccctacagt tgcctattct gtcctagtag tggcaaaagt tgagaaggga aaatccaaga
4561 aactgaagtc agtcaaagaa ttattgggga taacgattat ggagcgctcg tcttttgaaa
4621 agaaccccat cgacttcctt gaggcgaaag gttacaagga agtaaaaaag gatctcataa
4681 ttaaactacc aaagtatagt ctgtttgagt tagaaaatgg ccgaaaacgg atgttggcta
4741 gcgccggaga gcttcaaaag gggaacgaac tcgcactacc gtctaaatac gtgaatttcc
4801 tgtatttagc gtcccattac gagaagttga aaggttcacc tgaagataac gaacagaagc
4861 aactttttgt tgagcagcac aaacattatc tcgacgaaat catagagcaa atttcggaat
4921 tcagtaagag agtcatccta gctgatgcca atctggacaa agtattaagc gcatacaaca
4981 agcacaggga taaacccata cgtgagcagg cggaaaatat tatccatttg tttactctta
5041 ccaacctcgg cgctccagcc gcattcaagt attttgacac aacgatagat cgcaaacgat
5101 acacttctac caaggaggtg ctagacgcga cactgattca ccaatccatc acgggattat
5161 atgaaactcg gatagatttg tcacagcttg ggggtgactc tggtggttct cccaagaaga
5221 agaggaaagt ctaaccggtc atcatcacca tcaccattga gtttaaaccc gctgatcagc
5281 ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt
5341 gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca
5401 ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga
5461 ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc
5521 ggaaagaacc agctggggct cgataccgtc gacctctagc tagagcttgg cgtaatcatg
5581 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
5641 cggaagcata aagtgtaaag cctagggtgc ctaatgagtg agctaactca cattaattgc
5701 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
5761 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
5821 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
5881 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
5941 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
6001 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
6061 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
6121 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
6181 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
6241 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
6301 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
6361 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
6421 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
6481 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
6541 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
6601 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
6661 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
6721 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
6781 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
6841 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
6901 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
6961 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
7021 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
7081 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
7141 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
7201 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
7261 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
7321 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
7381 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
7441 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
7501 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
7561 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
7621 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
7681 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcga
7741 cggatcggga gatcgatctc ccgatcccct agggtcgact ctcagtacaa tctgctctga
7801 tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg
7861 cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca tgaagaatct
7921 gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata cgcgttgaca
7981 ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc atagcccata
8041 tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga
8101 cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt
8161 ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt
8221 gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca
8281 ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt
8341 catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt
8401 tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca
8461 ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg
8521 cggtaggcgt gtacggtggg aggtctatat aagcagagct ggtttagtga accgtcagat
8581 ccgctagaga tccgcggccg ctaatacgac tcactatagg gagagccgcc accatgtccg
8641 aagtcgagtt ttcccatgag tactggatga gacacgcatt gactctcgca aagagggctt
8701 gggatgaacg cgaggtgccc gtggggg
//
