| 质粒名称: | pCMV-ABE7.9 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 102918 |
| 存储实验室: | David Liu |
|
载体骨架基本信息 |
|
| 载体名称: | pBR322/CMV |
| 载体出品公司: | Liu lab |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 3388 bp |
| 完整质粒大小: | 8727 bp |
| 载体修饰: | -- |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | ABE7.9,TadA-linker(32aa)-TadA*(7.9)-linker(32aa)-nCas9-NLS |
| 物种来源: | Synthetic; TadA is from E.coli (synthetic gene block, mammalian codon optimized), Cas9 is S. pyogenes (mammalian codon optimized) |
| 插入大小: | 5328 bp |
| 基因突变: | see manuscript |
| GenBank编号 | -- |
| 启动子 | CMV |
| 标签/融合蛋白: | NLS（插接件上的C端子） |
| 克隆方法: | Ligation Independent克隆 |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | T7 |
| 3' 测序引物 | M13 |
|
文献引用方法 |
|
| 材料和方法部分: | pCMV-ABE7.9 was a gift from David Liu (Addgene plasmid # 102918 ; http://n2t.net/addgene:102918 ; RRID:Addgene_102918) |
| 参考文献部分: | Programmable base editing of A*T to G*C in genomic DNA without DNA cleavage. Gaudelli NM, Komor AC, Rees HA, Packer MS, Badran AH, Bryson DI, Liu DR. Nature. 2017 Nov 23;551(7681):464-471. doi: 10.1038/nature24644. Epub 2017 Oct 25. 10.1038/nature24644 PubMed 29160308 |
买家导航
pCMVABE7.9载体基本信息
pCMVABE7.9载体图谱质粒图谱和多克隆位点信息
pCMVABE7.9载体简介
pCMVABE7.9载体序列
LOCUS pCMV-ABE7.9 8727 bp ds-DNA circular SYN 22-AUG-2018
DEFINITION expresses ABE7.9 in mammalian cells.
ACCESSION .
VERSION .
KEYWORDS pCMV-ABE7.9
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8727)
AUTHORS Gaudelli NM, Komor AC, Rees HA, Packer MS, Badran AH, Bryson DI, Liu
DR
TITLE Programmable base editing of A*T to G*C in genomic DNA without DNA
cleavage.
JOURNAL Nature. 2017 Nov 23;551(7681):464-471. doi: 10.1038/nature24644.
Epub 2017 Oct 25.
PUBMED 29160308
REFERENCE 2 (bases 1 to 8727)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 22, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..8727
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind complement(50..69)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
enhancer 241..620
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 621..824
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 775..795
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind 866..885
/label=T7
/note="T7 promoter, forward primer"
promoter 866..884
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
CDS 2089..6189
/codon_start=1
/product="nickase mutant of the Cas9 endonuclease from the
Streptococcus pyogenes Type II CRISPR/Cas system"
/label=Cas9(D10A)
/note="generates RNA-guided single strand nicks in DNA due
to the D10A mutation in the RuvC catalytic domain"
/translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
ATLIHQSITGLYETRIDLSQLGGD"
CDS 6202..6222
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS 6231..6248
/codon_start=1
/product="6xHis affinity tag"
/label=6xHis
/translation="HHHHHH"
primer_bind complement(6271..6288)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 6277..6501
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
primer_bind complement(6572..6588)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(6572..6588)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(6585..6607)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 6596..6612
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(6620..6650)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 6665..6686
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(6803..6820)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(6974..7562)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(7054..7073)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(7733..8593)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 8356..8375
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(8594..8698)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 tcgacggatc gggagatcga tctcccgatc ccctagggtc gactctcagt acaatctgct
61 ctgatgccgc atagttaagc cagtatctgc tccctgcttg tgtgttggag gtcgctgagt
121 agtgcgcgag caaaatttaa gctacaacaa ggcaaggctt gaccgacaat tgcatgaaga
181 atctgcttag ggttaggcgt tttgcgctgc ttcgcgatgt acgggccaga tatacgcgtt
241 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
301 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
361 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
421 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
481 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
541 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
601 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
661 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
721 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
781 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctggttta gtgaaccgtc
841 agatccgcta gagatccgcg gccgctaata cgactcacta tagggagagc cgccaccatg
901 tccgaagtcg agttttccca tgagtactgg atgagacacg cattgactct cgcaaagagg
961 gcttgggatg aacgcgaggt gcccgtgggg gcagtactcg tgcataacaa tcgcgtaatc
1021 ggcgaaggtt ggaataggcc gatcggacgc cacgacccca ctgcacatgc ggaaatcatg
1081 gcccttcgac agggagggct tgtgatgcag aattatcgac ttatcgatgc gacgctgtac
1141 gtcacgcttg aaccttgcgt aatgtgcgcg ggagctatga ttcactcccg cattggacga
1201 gttgtattcg gtgcccgcga cgccaagacg ggtgccgcag gttcactgat ggacgtgctg
1261 catcacccag gcatgaacca ccgggtagaa atcacagaag gcatattggc ggacgaatgt
1321 gcggcgctgt tgtccgactt ttttcgcatg cggaggcagg agatcaaggc ccagaaaaaa
1381 gcacaatcct ctactgactc tggtggttct tctggtggtt ctagcggcag cgagactccc
1441 gggacctcag agtccgccac acccgaaagt tctggtggtt cttctggtgg ttcttccgaa
1501 gtcgagtttt cccatgagta ctggatgaga cacgcattga ctctcgcaaa gagggctctc
1561 gatgaacgcg aggtgcccgt gggggcagta ctcgtgctca acaatcgcgt aatcggcgaa
1621 ggttggaata gggcaatcgg actccacgac cccactgcac atgcggaaat catggccctt
1681 cgacagggag ggcttgtgat gcagaattat cgacttatcg atgcgacgct gtacgtcacg
1741 tttgaacctt gcgtaatgtg cgcgggagct atgattcact cccgcattgg acgagttgta
1801 ttcggtgttc gcaacgccaa gacgggtgcc gcaggttcac tgatggacgt gctgcattac
1861 ccaggcatga accaccgggt agaaatcaca gaaggcatat tggcggacga atgtaacgcg
1921 ctgttgtgtt acttttttcg catgcccagg caggtcttta acgcccagaa aaaagcacaa
1981 tcctctactg actctggtgg ttcttctggt ggttctagcg gcagcgagac tcccgggacc
2041 tcagagtccg ccacacccga aagttctggt ggttcttctg gtggttctga taaaaagtat
2101 tctattggtt tagccatcgg cactaattcc gttggatggg ctgtcataac cgatgaatac
2161 aaagtacctt caaagaaatt taaggtgttg gggaacacag accgtcattc gattaaaaag
2221 aatcttatcg gtgccctcct attcgatagt ggcgaaacgg cagaggcgac tcgcctgaaa
2281 cgaaccgctc ggagaaggta tacacgtcgc aagaaccgaa tatgttactt acaagaaatt
2341 tttagcaatg agatggccaa agttgacgat tctttctttc accgtttgga agagtccttc
2401 cttgtcgaag aggacaagaa acatgaacgg caccccatct ttggaaacat agtagatgag
2461 gtggcatatc atgaaaagta cccaacgatt tatcacctca gaaaaaagct agttgactca
2521 actgataaag cggacctgag gttaatctac ttggctcttg cccatatgat aaagttccgt
2581 gggcactttc tcattgaggg tgatctaaat ccggacaact cggatgtcga caaactgttc
2641 atccagttag tacaaaccta taatcagttg tttgaagaga accctataaa tgcaagtggc
2701 gtggatgcga aggctattct tagcgcccgc ctctctaaat cccgacggct agaaaacctg
2761 atcgcacaat tacccggaga gaagaaaaat gggttgttcg gtaaccttat agcgctctca
2821 ctaggcctga caccaaattt taagtcgaac ttcgacttag ctgaagatgc caaattgcag
2881 cttagtaagg acacgtacga tgacgatctc gacaatctac tggcacaaat tggagatcag
2941 tatgcggact tatttttggc tgccaaaaac cttagcgatg caatcctcct atctgacata
3001 ctgagagtta atactgagat taccaaggcg ccgttatccg cttcaatgat caaaaggtac
3061 gatgaacatc accaagactt gacacttctc aaggccctag tccgtcagca actgcctgag
3121 aaatataagg aaatattctt tgatcagtcg aaaaacgggt acgcaggtta tattgacggc
3181 ggagcgagtc aagaggaatt ctacaagttt atcaaaccca tattagagaa gatggatggg
3241 acggaagagt tgcttgtaaa actcaatcgc gaagatctac tgcgaaagca gcggactttc
3301 gacaacggta gcattccaca tcaaatccac ttaggcgaat tgcatgctat acttagaagg
3361 caggaggatt tttatccgtt cctcaaagac aatcgtgaaa agattgagaa aatcctaacc
3421 tttcgcatac cttactatgt gggacccctg gcccgaggga actctcggtt cgcatggatg
3481 acaagaaagt ccgaagaaac gattactcca tggaattttg aggaagttgt cgataaaggt
3541 gcgtcagctc aatcgttcat cgagaggatg accaactttg acaagaattt accgaacgaa
3601 aaagtattgc ctaagcacag tttactttac gagtatttca cagtgtacaa tgaactcacg
3661 aaagttaagt atgtcactga gggcatgcgt aaacccgcct ttctaagcgg agaacagaag
3721 aaagcaatag tagatctgtt attcaagacc aaccgcaaag tgacagttaa gcaattgaaa
3781 gaggactact ttaagaaaat tgaatgcttc gattctgtcg agatctccgg ggtagaagat
3841 cgatttaatg cgtcacttgg tacgtatcat gacctcctaa agataattaa agataaggac
3901 ttcctggata acgaagagaa tgaagatatc ttagaagata tagtgttgac tcttaccctc
3961 tttgaagatc gggaaatgat tgaggaaaga ctaaaaacat acgctcacct gttcgacgat
4021 aaggttatga aacagttaaa gaggcgtcgc tatacgggct ggggacgatt gtcgcggaaa
4081 cttatcaacg ggataagaga caagcaaagt ggtaaaacta ttctcgattt tctaaagagc
4141 gacggcttcg ccaataggaa ctttatgcag ctgatccatg atgactcttt aaccttcaaa
4201 gaggatatac aaaaggcaca ggtttccgga caaggggact cattgcacga acatattgcg
4261 aatcttgctg gttcgccagc catcaaaaag ggcatactcc agacagtcaa agtagtggat
4321 gagctagtta aggtcatggg acgtcacaaa ccggaaaaca ttgtaatcga gatggcacgc
4381 gaaaatcaaa cgactcagaa ggggcaaaaa aacagtcgag agcggatgaa gagaatagaa
4441 gagggtatta aagaactggg cagccagatc ttaaaggagc atcctgtgga aaatacccaa
4501 ttgcagaacg agaaacttta cctctattac ctacaaaatg gaagggacat gtatgttgat
4561 caggaactgg acataaaccg tttatctgat tacgacgtcg atcacattgt accccaatcc
4621 tttttgaagg acgattcaat cgacaataaa gtgcttacac gctcggataa gaaccgaggg
4681 aaaagtgaca atgttccaag cgaggaagtc gtaaagaaaa tgaagaacta ttggcggcag
4741 ctcctaaatg cgaaactgat aacgcaaaga aagttcgata acttaactaa agctgagagg
4801 ggtggcttgt ctgaacttga caaggccgga tttattaaac gtcagctcgt ggaaacccgc
4861 caaatcacaa agcatgttgc acagatacta gattcccgaa tgaatacgaa atacgacgag
4921 aacgataagc tgattcggga agtcaaagta atcactttaa agtcaaaatt ggtgtcggac
4981 ttcagaaagg attttcaatt ctataaagtt agggagataa ataactacca ccatgcgcac
5041 gacgcttatc ttaatgccgt cgtagggacc gcactcatta agaaataccc gaagctagaa
5101 agtgagtttg tgtatggtga ttacaaagtt tatgacgtcc gtaagatgat cgcgaaaagc
5161 gaacaggaga taggcaaggc tacagccaaa tacttctttt attctaacat tatgaatttc
5221 tttaagacgg aaatcactct ggcaaacgga gagatacgca aacgaccttt aattgaaacc
5281 aatggggaga caggtgaaat cgtatgggat aagggccggg acttcgcgac ggtgagaaaa
5341 gttttgtcca tgccccaagt caacatagta aagaaaactg aggtgcagac cggagggttt
5401 tcaaaggaat cgattcttcc aaaaaggaat agtgataagc tcatcgctcg taaaaaggac
5461 tgggacccga aaaagtacgg tggcttcgat agccctacag ttgcctattc tgtcctagta
5521 gtggcaaaag ttgagaaggg aaaatccaag aaactgaagt cagtcaaaga attattgggg
5581 ataacgatta tggagcgctc gtcttttgaa aagaacccca tcgacttcct tgaggcgaaa
5641 ggttacaagg aagtaaaaaa ggatctcata attaaactac caaagtatag tctgtttgag
5701 ttagaaaatg gccgaaaacg gatgttggct agcgccggag agcttcaaaa ggggaacgaa
5761 ctcgcactac cgtctaaata cgtgaatttc ctgtatttag cgtcccatta cgagaagttg
5821 aaaggttcac ctgaagataa cgaacagaag caactttttg ttgagcagca caaacattat
5881 ctcgacgaaa tcatagagca aatttcggaa ttcagtaaga gagtcatcct agctgatgcc
5941 aatctggaca aagtattaag cgcatacaac aagcacaggg ataaacccat acgtgagcag
6001 gcggaaaata ttatccattt gtttactctt accaacctcg gcgctccagc cgcattcaag
6061 tattttgaca caacgataga tcgcaaacga tacacttcta ccaaggaggt gctagacgcg
6121 acactgattc accaatccat cacgggatta tatgaaactc ggatagattt gtcacagctt
6181 gggggtgact ctggtggttc tcccaagaag aagaggaaag tctaaccggt catcatcacc
6241 atcaccattg agtttaaacc cgctgatcag cctcgactgt gccttctagt tgccagccat
6301 ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc
6361 tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg
6421 ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg
6481 gggatgcggt gggctctatg gcttctgagg cggaaagaac cagctggggc tcgataccgt
6541 cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
6601 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctagggtg
6661 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
6721 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
6781 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
6841 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
6901 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
6961 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
7021 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
7081 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
7141 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
7201 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
7261 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
7321 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
7381 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
7441 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
7501 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
7561 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
7621 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
7681 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
7741 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
7801 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
7861 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
7921 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
7981 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
8041 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
8101 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
8161 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
8221 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
8281 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
8341 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
8401 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
8461 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
8521 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
8581 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
8641 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
8701 catttccccg aaaagtgcca cctgacg
//
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