| 质粒名称: | pCMV-BE1 |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 73019 |
| 存储实验室: | David Liu |
|
载体骨架基本信息 |
|
| 载体名称: | CMV |
| 载体出品公司: | origene |
| 空载体大小: | 3399 bp |
| 完整质粒大小: | 8271 bp |
| 载体修饰: | -- |
| 载体类型: | 哺乳动物表达 |
| 筛选标记: | -- |
| 载体抗性: | 氨苄青霉素，100μg/mL |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 低拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | BE1 |
| 物种来源: | 褐家鼠；化脓性链球菌 |
| 插入大小: | 4872 bp |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子: | CMV |
| 标签/融合蛋白: | -- |
| 克隆方法: | Ligation Independent Cloning |
| 5’克隆位点: | -- |
| 3’克隆位点: | -- |
| 5’测序引物 | CMV |
| 3’测序引物 | TGGTTCTTTCCGCCTCAGAAGC |
|
文献引用方法 |
|
| 材料和方法部分: | pCMV-BE1 was a gift from David Liu (Addgene plasmid # 73019 ; http://n2t.net/addgene:73019 ; RRID:Addgene_73019) |
| 参考文献部分: | Programmable editing of a target base in genomic DNA without double-stranded DNA cleavage. Komor AC, Kim YB, Packer MS, Zuris JA, Liu DR. Nature. 2016 Apr 20. doi: 10.1038/nature17946. 10.1038/nature17946 PubMed 27096365 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pCMV-BE1 8271 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Expresses BE1 with C-terminal NLS in mammalian cells.
ACCESSION .
VERSION .
KEYWORDS pCMV-BE1
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8271)
AUTHORS Komor AC, Kim YB, Packer MS, Zuris JA, Liu DR
TITLE Programmable editing of a target base in genomic DNA without
double-stranded DNA cleavage.
JOURNAL Nature. 2016 Apr 20. doi: 10.1038/nature17946.
PUBMED 27096365
REFERENCE 2 (bases 1 to 8271)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..8271
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 120..499
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 500..703
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 654..674
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind 745..764
/label=T7
/note="T7 promoter, forward primer"
promoter 745..763
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
CDS 777..1463
/codon_start=1
/product="cytidine deaminase (C to U editing enzyme) from
rat"
/label=APOBEC-1
/note="can use ssDNA as a substrate (Komor et al., 2016)"
/translation="MSSETGPVAVDPTLRRRIEPHEFEVFFDPRELRKETCLLYEINWG
GRHSIWRHTSQNTNKHVEVNFIEKFTTERYFCPNTRCSITWFLSWSPCGECSRAITEFL
SRYPHVTLFIYIARLYHHADPRNRQGLRDLISSGVTIQIMTEQESGYCWRNFVNYSPSN
EAHWPRYPHLWVRLYVLELYCIILGLPPCLNILRRKQPQLTFFTIALQSCHYQRLPPHI
LWATGLK"
CDS 1512..5612
/codon_start=1
/product="catalytically dead mutant of the Cas9
endonuclease from the Streptococcus pyogenes Type II
CRISPR/Cas system"
/label=dCas9
/note="RNA-guided DNA-binding protein that lacks
endonuclease activity due to the D10A mutation in the RuvC
catalytic domain and the H840A mutation in the HNH
catalytic domain"
/translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
ATLIHQSITGLYETRIDLSQLGGD"
CDS 5625..5645
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS 5654..5671
/codon_start=1
/product="6xHis affinity tag"
/label=6xHis
/translation="HHHHHH"
primer_bind complement(5694..5711)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 5700..5924
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
primer_bind complement(5995..6011)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(5995..6011)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(6008..6030)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 6019..6035
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(6043..6073)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 6088..6109
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(6226..6243)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(6397..6985)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(6477..6496)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(7156..8016)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 7779..7798
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(8017..8121)
/gene="bla"
/label=AmpR promoter
primer_bind complement(8200..8219)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
ORIGIN
1 gtgcgcgagc aaaatttaag ctacaacaag gcaaggcttg accgacaatt gcatgaagaa
61 tctgcttagg gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg
121 acattgatta ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc
181 atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa
241 cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac
301 tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca
361 agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg
421 gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt
481 agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg
541 gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg
601 gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat
661 gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctggtttag tgaaccgtca
721 gatccgctag agatccgcgg ccgctaatac gactcactat agggagagcc gccaccatga
781 gctcagagac tggcccagtg gctgtggacc ccacattgag acggcggatc gagccccatg
841 agtttgaggt attcttcgat ccgagagagc tccgcaagga gacctgcctg ctttacgaaa
901 ttaattgggg gggccggcac tccatttggc gacatacatc acagaacact aacaagcacg
961 tcgaagtcaa cttcatcgag aagttcacga cagaaagata tttctgtccg aacacaaggt
1021 gcagcattac ctggtttctc agctggagcc catgcggcga atgtagtagg gccatcactg
1081 aattcctgtc aaggtatccc cacgtcactc tgtttattta catcgcaagg ctgtaccacc
1141 acgctgaccc ccgcaatcga caaggcctgc gggatttgat ctcttcaggt gtgactatcc
1201 aaattatgac tgagcaggag tcaggatact gctggagaaa ctttgtgaat tatagcccga
1261 gtaatgaagc ccactggcct aggtatcccc atctgtgggt acgactgtac gttcttgaac
1321 tgtactgcat catactgggc ctgcctcctt gtctcaacat tctgagaagg aagcagccac
1381 agctgacatt ctttaccatc gctcttcagt cttgtcatta ccagcgactg cccccacaca
1441 ttctctgggc caccgggttg aaaagcggca gcgagactcc cgggacctca gagtccgcca
1501 cacccgaaag tgataaaaag tattctattg gtttagccat cggcactaat tccgttggat
1561 gggctgtcat aaccgatgaa tacaaagtac cttcaaagaa atttaaggtg ttggggaaca
1621 cagaccgtca ttcgattaaa aagaatctta tcggtgccct cctattcgat agtggcgaaa
1681 cggcagaggc gactcgcctg aaacgaaccg ctcggagaag gtatacacgt cgcaagaacc
1741 gaatatgtta cttacaagaa atttttagca atgagatggc caaagttgac gattctttct
1801 ttcaccgttt ggaagagtcc ttccttgtcg aagaggacaa gaaacatgaa cggcacccca
1861 tctttggaaa catagtagat gaggtggcat atcatgaaaa gtacccaacg atttatcacc
1921 tcagaaaaaa gctagttgac tcaactgata aagcggacct gaggttaatc tacttggctc
1981 ttgcccatat gataaagttc cgtgggcact ttctcattga gggtgatcta aatccggaca
2041 actcggatgt cgacaaactg ttcatccagt tagtacaaac ctataatcag ttgtttgaag
2101 agaaccctat aaatgcaagt ggcgtggatg cgaaggctat tcttagcgcc cgcctctcta
2161 aatcccgacg gctagaaaac ctgatcgcac aattacccgg agagaagaaa aatgggttgt
2221 tcggtaacct tatagcgctc tcactaggcc tgacaccaaa ttttaagtcg aacttcgact
2281 tagctgaaga tgccaaattg cagcttagta aggacacgta cgatgacgat ctcgacaatc
2341 tactggcaca aattggagat cagtatgcgg acttattttt ggctgccaaa aaccttagcg
2401 atgcaatcct cctatctgac atactgagag ttaatactga gattaccaag gcgccgttat
2461 ccgcttcaat gatcaaaagg tacgatgaac atcaccaaga cttgacactt ctcaaggccc
2521 tagtccgtca gcaactgcct gagaaatata aggaaatatt ctttgatcag tcgaaaaacg
2581 ggtacgcagg ttatattgac ggcggagcga gtcaagagga attctacaag tttatcaaac
2641 ccatattaga gaagatggat gggacggaag agttgcttgt aaaactcaat cgcgaagatc
2701 tactgcgaaa gcagcggact ttcgacaacg gtagcattcc acatcaaatc cacttaggcg
2761 aattgcatgc tatacttaga aggcaggagg atttttatcc gttcctcaaa gacaatcgtg
2821 aaaagattga gaaaatccta acctttcgca taccttacta tgtgggaccc ctggcccgag
2881 ggaactctcg gttcgcatgg atgacaagaa agtccgaaga aacgattact ccatggaatt
2941 ttgaggaagt tgtcgataaa ggtgcgtcag ctcaatcgtt catcgagagg atgaccaact
3001 ttgacaagaa tttaccgaac gaaaaagtat tgcctaagca cagtttactt tacgagtatt
3061 tcacagtgta caatgaactc acgaaagtta agtatgtcac tgagggcatg cgtaaacccg
3121 cctttctaag cggagaacag aagaaagcaa tagtagatct gttattcaag accaaccgca
3181 aagtgacagt taagcaattg aaagaggact actttaagaa aattgaatgc ttcgattctg
3241 tcgagatctc cggggtagaa gatcgattta atgcgtcact tggtacgtat catgacctcc
3301 taaagataat taaagataag gacttcctgg ataacgaaga gaatgaagat atcttagaag
3361 atatagtgtt gactcttacc ctctttgaag atcgggaaat gattgaggaa agactaaaaa
3421 catacgctca cctgttcgac gataaggtta tgaaacagtt aaagaggcgt cgctatacgg
3481 gctggggacg attgtcgcgg aaacttatca acgggataag agacaagcaa agtggtaaaa
3541 ctattctcga ttttctaaag agcgacggct tcgccaatag gaactttatg cagctgatcc
3601 atgatgactc tttaaccttc aaagaggata tacaaaaggc acaggtttcc ggacaagggg
3661 actcattgca cgaacatatt gcgaatcttg ctggttcgcc agccatcaaa aagggcatac
3721 tccagacagt caaagtagtg gatgagctag ttaaggtcat gggacgtcac aaaccggaaa
3781 acattgtaat cgagatggca cgcgaaaatc aaacgactca gaaggggcaa aaaaacagtc
3841 gagagcggat gaagagaata gaagagggta ttaaagaact gggcagccag atcttaaagg
3901 agcatcctgt ggaaaatacc caattgcaga acgagaaact ttacctctat tacctacaaa
3961 atggaaggga catgtatgtt gatcaggaac tggacataaa ccgtttatct gattacgacg
4021 tcgatgccat tgtaccccaa tcctttttga aggacgattc aatcgacaat aaagtgctta
4081 cacgctcgga taagaaccga gggaaaagtg acaatgttcc aagcgaggaa gtcgtaaaga
4141 aaatgaagaa ctattggcgg cagctcctaa atgcgaaact gataacgcaa agaaagttcg
4201 ataacttaac taaagctgag aggggtggct tgtctgaact tgacaaggcc ggatttatta
4261 aacgtcagct cgtggaaacc cgccaaatca caaagcatgt tgcacagata ctagattccc
4321 gaatgaatac gaaatacgac gagaacgata agctgattcg ggaagtcaaa gtaatcactt
4381 taaagtcaaa attggtgtcg gacttcagaa aggattttca attctataaa gttagggaga
4441 taaataacta ccaccatgcg cacgacgctt atcttaatgc cgtcgtaggg accgcactca
4501 ttaagaaata cccgaagcta gaaagtgagt ttgtgtatgg tgattacaaa gtttatgacg
4561 tccgtaagat gatcgcgaaa agcgaacagg agataggcaa ggctacagcc aaatacttct
4621 tttattctaa cattatgaat ttctttaaga cggaaatcac tctggcaaac ggagagatac
4681 gcaaacgacc tttaattgaa accaatgggg agacaggtga aatcgtatgg gataagggcc
4741 gggacttcgc gacggtgaga aaagttttgt ccatgcccca agtcaacata gtaaagaaaa
4801 ctgaggtgca gaccggaggg ttttcaaagg aatcgattct tccaaaaagg aatagtgata
4861 agctcatcgc tcgtaaaaag gactgggacc cgaaaaagta cggtggcttc gatagcccta
4921 cagttgccta ttctgtccta gtagtggcaa aagttgagaa gggaaaatcc aagaaactga
4981 agtcagtcaa agaattattg gggataacga ttatggagcg ctcgtctttt gaaaagaacc
5041 ccatcgactt ccttgaggcg aaaggttaca aggaagtaaa aaaggatctc ataattaaac
5101 taccaaagta tagtctgttt gagttagaaa atggccgaaa acggatgttg gctagcgccg
5161 gagagcttca aaaggggaac gaactcgcac taccgtctaa atacgtgaat ttcctgtatt
5221 tagcgtccca ttacgagaag ttgaaaggtt cacctgaaga taacgaacag aagcaacttt
5281 ttgttgagca gcacaaacat tatctcgacg aaatcataga gcaaatttcg gaattcagta
5341 agagagtcat cctagctgat gccaatctgg acaaagtatt aagcgcatac aacaagcaca
5401 gggataaacc catacgtgag caggcggaaa atattatcca tttgtttact cttaccaacc
5461 tcggcgctcc agccgcattc aagtattttg acacaacgat agatcgcaaa cgatacactt
5521 ctaccaagga ggtgctagac gcgacactga ttcaccaatc catcacggga ttatatgaaa
5581 ctcggataga tttgtcacag cttgggggtg actctggtgg ttctcccaag aagaagagga
5641 aagtctaacc ggtcatcatc accatcacca ttgagtttaa acccgctgat cagcctcgac
5701 tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct
5761 ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct
5821 gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg
5881 ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaag
5941 aaccagctgg ggctcgatac cgtcgacctc tagctagagc ttggcgtaat catggtcata
6001 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
6061 cataaagtgt aaagcctagg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
6121 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
6181 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
6241 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
6301 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
6361 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
6421 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
6481 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
6541 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
6601 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
6661 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
6721 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
6781 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
6841 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
6901 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
6961 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
7021 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
7081 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
7141 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
7201 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
7261 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
7321 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
7381 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
7441 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
7501 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
7561 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
7621 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
7681 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
7741 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
7801 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
7861 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
7921 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
7981 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
8041 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
8101 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc
8161 gggagatcga tctcccgatc ccctagggtc gactctcagt acaatctgct ctgatgccgc
8221 atagttaagc cagtatctgc tccctgcttg tgtgttggag gtcgctgagt a
//
