| 质粒名称: | pCMVβ-p300-myc |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 30489 |
| 存储实验室: | Tso-Pang Yao |
|
载体骨架基本信息 |
|
| 载体名称: | pCMVb |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 4800 bp |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | p300 |
| 物种来源: | H. sapiens (human) |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | Myc（插入的C端子） |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | CMV-Fwd |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pCMVβ-p300-myc was a gift from Tso-Pang Yao (Addgene plasmid # 30489 ; http://n2t.net/addgene:30489 ; RRID:Addgene_30489) |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pCMV-beta-p300-myc 11893 bp ds-DNA circular SYN 17-MAY-2019
DEFINITION Mammalian expression of human p300 with myc tag.
ACCESSION .
VERSION .
KEYWORDS pCMV-beta-p300-myc
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 11893)
TITLE p300 plasmids
REFERENCE 2 (bases 1 to 11893)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 17, 2019 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..11893
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind complement(30..52)
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind 152..171
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 385..404
/label=pBRrevBam
/note="pBR322 vectors, tet region, downstream of BamHI,
reverse primer"
promoter 694..712
/label=SP6 promoter
/note="promoter for bacteriophage SP6 RNA polymerase"
primer_bind 694..711
/label=SP6
/note="SP6 promoter, forward primer"
enhancer 736..1039
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 1040..1243
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 1194..1214
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind 1240..1264
/label=LNCX
/note="Human CMV promoter, forward primer"
primer_bind 1345..1361
/label=SK primer
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 1345..1361
/label=pBluescriptSK
/note="For pBluescript vector"
CDS 4572..6422
/codon_start=1
/gene="EP300"
/product="catalytic histone acetyltransferase core of the
human E1A-associated protein p300 (Delvecchio et al.,
2013)"
/label=p300 core
/translation="IFKPEELRQALMPTLEALYRQDPESLPFRQPVDPQLLGIPDYFDI
VKSPMDLSTIKRKLDTGQYQEPWQYVDDIWLMFNNAWLYNRKTSRVYKYCSKLSEVFEQ
EIDPVMQSLGYCCGRKLEFSPQTLCCYGKQLCTIPRDATYYSYQNRYHFCEKCFNEIQG
ESVSLGDDPSQPQTTINKEQFSKRKNDTLDPELFVECTECGRKMHQICVLHHEIIWPAG
FVCDGCLKKSARTRKENKFSAKRLPSTRLGTFLENRVNDFLRRQNHPESGEVTVRVVHA
SDKTVEVKPGMKARFVDSGEMAESFPYRTKALFAFEEIDGVDLCFFGMHVQEYGSDCPP
PNQRRVYISYLDSVHFFRPKCLRTAVYHEILIGYLEYVKKLGYTTGHIWACPPSEGDDY
IFHCHPPDQKIPKPKRLQEWYKKMLDKAVSERIVHDYKDIFKQATEDRLTSAKELPYFE
GDFWPNVLEESIKELEQEEEERKREENTSNESTDVTKGDSKNAKKKNNKKTSKNKSSLS
RGNKKKPGMPNVSNDLSQKLYATMEKHKEVFFVIRLIAGPAANSLPPIVDPDPLIPCDL
MDGRDAFLTLARDKHLEFSSLRRAQWSTMCMLVELHTQSQD"
regulatory 6186..6195
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 8673..8702
/codon_start=1
/product="Myc (human c-Myc proto-oncogene) epitope tag"
/label=Myc
/translation="EQKLISEEDL"
intron 8761..9333
/label=-beta-globin intron
/note="intron from rabbit beta-globin gene"
primer_bind complement(9465..9484)
/label=Bglob-pA-R
/note="Rabbit beta-globin polyA region, reverse primer"
polyA_site 9530..9585
/label=-beta-globin poly(A) signal
/note="rabbit beta-globin polyadenylation signal"
primer_bind complement(9584..9603)
/label=rbglobpA-R
/note="Rabbit beta-globin polyA, reverse primer. Also
called rb-glob-pA-term-R"
primer_bind complement(9904..9921)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(10075..10663)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(10155..10174)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(10834..11694)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 11457..11476
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(11695..11799)
/gene="bla"
/label=AmpR promoter
primer_bind 11867..11885
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
ORIGIN
1 ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc
61 acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt
121 gttggcgggt gtcggggctg gcttaactat gcggcatcag agcagattgt actgagagtg
181 caccattcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt
241 gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag
301 tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa
361 gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc
421 tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatctggc tagcgatgac
481 cctgctgatt ggttcgctga ccatttccgg gtgcgggacg gcgttaccag aaactcagaa
541 ggttcgtcca accaaaccga ctctgacggc agtttacgag agagatgata gggtctgctt
601 cagtaagcca gatgctacac aattaggctt gtacatattg tcgttagaac gcggctacaa
661 ttaatacata accttatgta tcatacacat acgatttagg tgacactata gaatacacgg
721 aattcgagct cgccccgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa
781 cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac
841 tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca
901 agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg
961 gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt
1021 agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg
1081 gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg
1141 gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat
1201 gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctcgtttag tgaaccgtca
1261 gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagacacc gggaccgatc
1321 cagcctccgg gggatcgcgg ccgctctaga actagtggat cccccgggcc gaagaagaga
1381 tttcctgagg attctggttt tcctcgcttg tatctccgaa agaattaaaa atggccgaga
1441 atgtggtgga accggggccg ccttcagcca agcggcctaa actctcatct ccggccctct
1501 cggcgtccgc cagcgatggc acagattttg gctctctatt tgacttggag cacgacttac
1561 cagatgaatt aatcaactct acagaattgg gactaaccaa tggtggtgat attaatcagc
1621 ttcagacaag tcttggcatg gtacaagatg cagcttctaa acataaacag ctgtcagaat
1681 tgctgcgatc tggtagttcc cctaacctca atatgggagt tggtggccca ggtcaagtca
1741 tggccagcca ggcccaacag agcagtcctg gattaggttt gataaatagc atggtcaaaa
1801 gcccaatgac acaggcaggc ttgacttctc ccaacatggg gatgggcact agtggaccaa
1861 atcagggtcc tacgcagtca acaggtatga tgaacagtcc agtaaatcag cctgccatgg
1921 gaatgaacac agggatgaat gcgggcatga atcctggaat gttggctgca ggcaatggac
1981 aagggataat gcctaatcaa gtcatgaacg gttcaattgg agcaggccga gggcgacaga
2041 atatgcagta cccaaaccca ggcatgggaa gtgctggcaa cttactgact gagcctcttc
2101 agcagggctc tccccagatg ggaggacaaa caggattgag aggcccccag cctcttaaga
2161 tgggaatgat gaacaacccc aatccttatg gttcaccata tactcagaat cctggacagc
2221 agattggagc cagtggcctt ggtctccaga ttcagacaaa aactgtacta tcaaataact
2281 tatctccatt tgctatggac aaaaaggcag ttcctggtgg aggaatgccc aacatgggtc
2341 aacagccagc cccgcaggtc cagcagccag gtctggtgac tccagttgcc caagggatgg
2401 gttctggagc acatacagct gatccagaga agcgcaagct catccagcag cagcttgttc
2461 tccttttgca tgctcacaag tgccagcgcc gggaacaggc caatggggaa gtgaggcagt
2521 gcaaccttcc ccactgtcgc acaatgaaga atgtcctaaa ccacatgaca cactgccagt
2581 caggcaagtc ttgccaagtg gcacactgtg catcttctcg acaaatcatt tcacactgga
2641 agaattgtac aagacatgat tgtcctgtgt gtctccccct caaaaatgct ggtgataaga
2701 gaaatcaaca gccaattttg actggagcac ccgttggact tggaaatcct agctctctag
2761 gggtgggtca acagtctgcc cccaacctaa gcactgttag tcagattgat cccagctcca
2821 tagaaagagc ctatgcagct cttggactac cctatcaagt aaatcagatg ccgacacaac
2881 cccaggtgca agcaaagaac cagcagaatc agcagcctgg gcagtctccc caaggcatgc
2941 ggcccatgag caacatgagt gctagtccta tgggagtaaa tggaggtgta ggagttcaaa
3001 cgccgagtct tctttctgac tcaatgttgc attcagccat aaattctcaa aacccaatga
3061 tgagtgaaaa tgccagtgtg ccctccctgg gtcctatgcc aacagcagct caaccatcca
3121 ctactggaat tcggaaacag tggcacgaag atattactca ggatcttcga aatcatcttg
3181 ttcacaaact cgtccaagcc atatttccta cgccggatcc tgctgcttta aaagacagac
3241 ggatggaaaa cctagttgca tatgctcgga aagttgaagg ggacatgtat gaatctgcaa
3301 acaatcgagc ggaatactac caccttctag ctgagaaaat ctataagatc cagaaagaac
3361 tagaagaaaa acgaaggacc agactacaga agcagaacat gctaccaaat gctgcaggca
3421 tggttccagt ttccatgaat ccagggccta acatgggaca gccgcaacca ggaatgactt
3481 ctaatggccc tctacctgac ccaagtatga tccgtggcag tgtgccaaac cagatgatgc
3541 ctcgaataac tccacaatct ggtttgaatc aatttggcca gatgagcatg gcccagcccc
3601 ctattgtacc ccggcaaacc cctcctcttc agcaccatgg acagttggct caacctggag
3661 ctctcaaccc gcctatgggc tatgggcctc gtatgcaaca gccttccaac cagggccagt
3721 tccttcctca gactcagttc ccatcacagg gaatgaatgt aacaaatatc cctttggctc
3781 cgtccagcgg tcaagctcca gtgtctcaag cacaaatgtc tagttcttcc tgcccggtga
3841 actctcctat aatgcctcca gggtctcagg ggagccacat tcactgtccc cagcttcctc
3901 aaccagctct tcatcagaat tcaccctcgc ctgtacctag tcgtaccccc acccctcacc
3961 atactccccc aagcataggg gctcagcagc caccagcaac aacaattcca gcccctgttc
4021 ctacacctcc tgccatgcca cctgggccac agtcccaggc tctacatccc cctccaaggc
4081 agacacctac accaccaaca acacaacttc cccaacaagt gcagccttca cttcctgctg
4141 caccttctgc tgaccagccc cagcagcagc ctcgctcaca gcagagcaca gcagcgtctg
4201 ttcctacccc aacagcaccg ctgcttcctc cgcagcctgc aactccactt tcccagccag
4261 ctgtaagcat tgaaggacag gtatcaaatc ctccatctac tagtagcaca gaagtgaatt
4321 ctcaggccat tgctgagaag cagccttccc aggaagtgaa gatggaggcc aaaatggaag
4381 tggatcaacc agaaccagca gatactcagc cggaggatat ttcagagtct aaagtggaag
4441 actgtaaaat ggaatctacc gaaacagaag agagaagcac tgagttaaaa actgaaataa
4501 aagaggagga agaccagcca agtacttcag ctacccagtc atctccggct ccaggacagt
4561 caaagaaaaa gattttcaaa ccagaagaac tacgacaggc actgatgcca actttggagg
4621 cactttaccg tcaggatcca gaatcccttc cctttcgtca acctgtggac cctcagcttt
4681 taggaatccc tgattacttt gatattgtga agagccccat ggatctttct accattaaga
4741 ggaagttaga cactggacag tatcaggagc cctggcagta tgtcgatgat atttggctta
4801 tgttcaataa tgcctggtta tataaccgga aaacatcacg ggtatacaaa tactgctcca
4861 agctctctga ggtctttgaa caagaaattg acccagtgat gcaaagcctt ggatactgtt
4921 gtggcagaaa gttggagttc tctccacaga cactgtgttg ctacggcaaa cagttgtgca
4981 caatacctcg tgatgccact tattacagtt accagaacag gtatcatttc tgtgagaagt
5041 gtttcaatga gatccaaggg gagagcgttt ctttggggga tgacccttcc cagcctcaaa
5101 ctacaataaa taaagaacaa ttttccaaga gaaaaaatga cacactggat cctgaactgt
5161 ttgttgaatg tacagagtgc ggaagaaaga tgcatcagat ctgtgtcctt caccatgaga
5221 tcatctggcc tgctggattc gtctgtgatg gctgtttaaa gaaaagtgca cgaactagga
5281 aagaaaataa gttttctgct aaaaggttgc catctaccag acttggcacc tttctagaga
5341 atcgtgtgaa tgactttctg aggcgacaga atcaccctga gtcaggagag gtcactgtta
5401 gagtagttca tgcttctgac aaaaccgtgg aagtaaaacc aggcatgaaa gcaaggtttg
5461 tggacagtgg agagatggca gaatcctttc cataccgaac caaagccctc tttgcctttg
5521 aagaaattga tggtgttgac ctgtgcttct ttggcatgca tgttcaagag tatggctctg
5581 actgccctcc acccaaccag aggagagtat acatatctta cctcgatagt gttcatttct
5641 tccgtcctaa atgcttgagg actgcagtct atcatgaaat cctaattgga tatttagaat
5701 atgtcaagaa attaggttac acaacagggc atatttgggc atgtccacca agtgagggag
5761 atgattatat cttccattgc catcctcctg accagaagat acccaagccc aagcgactgc
5821 aggaatggta caaaaaaatg cttgacaagg ctgtatcaga gcgtattgtc catgactaca
5881 aggatatttt taaacaagct actgaagata gattaacaag tgcaaaggaa ttgccttatt
5941 tcgagggtga tttctggccc aatgttctgg aagaaagcat taaggaactg gaacaggagg
6001 aagaagagag aaaacgagag gaaaacacca gcaatgaaag cacagatgtg accaagggag
6061 acagcaaaaa tgctaaaaag aagaataata agaaaaccag caaaaataag agcagcctga
6121 gtaggggcaa caagaagaaa cccgggatgc ccaatgtatc taacgacctc tcacagaaac
6181 tatatgccac catggagaag cataaagagg tcttctttgt gatccgcctc attgctggcc
6241 ctgctgccaa ctccctgcct cccattgttg atcctgatcc tctcatcccc tgcgatctga
6301 tggatggtcg ggatgcgttt ctcacgctgg caagggacaa gcacctggag ttctcttcac
6361 tccgaagagc ccagtggtcc accatgtgca tgctggtgga gctgcacacg cagagccagg
6421 accgctttgt ctacacctgc aatgaatgca agcaccatgt ggagacacgc tggcactgta
6481 ctgtctgtga ggattatgac ttgtgtatca cctgctataa cactaaaaac catgaccaca
6541 aaatggagaa actaggcctt ggcttagatg atgagagcaa caaccagcag gctgcagcca
6601 cccagagccc aggcgattct cgccgcctga gtatccagcg ctgcatccag tctctggtcc
6661 atgcttgcca gtgtcggaat gccaattgct cactgccatc ctgccagaag atgaagcggg
6721 ttgtgcagca taccaagggt tgcaaacgga aaaccaatgg cgggtgcccc atctgcaagc
6781 agctcattgc cctctgctgc taccatgcca agcactgcca ggagaacaaa tgcccggtgc
6841 cgttctgcct aaacatcaag cagaagctcc ggcagcaaca gctgcagcac cgactacagc
6901 aggcccaaat gcttcgcagg aggatggcca gcatgcagcg gactggtgtg gttgggcagc
6961 aacagggcct cccttccccc actcctgcca ctccaacgac accaactggc caacagccaa
7021 ccaccccgca gacgccccag cccacttctc agcctcagcc tacccctccc aatagcatgc
7081 caccctactt gcccaggact caagctgctg gccctgtgtc ccagggtaag gcagcaggcc
7141 aggtgacccc tccaacccct cctcagactg ctcagccacc ccttccaggg cccccacctg
7201 cagcagtgga aatggcaatg cagattcaga gagcagcgga gacgcagcgc cagatggccc
7261 acgtgcaaat ttttcaaagg ccaatccaac accagatgcc cccgatgact cccatggccc
7321 ccatgggtat gaacccacct cccatgacca gaggtcccag tgggcatttg gagccaggga
7381 tgggaccgac agggatgcag caacagccac cctggagcca aggaggattg cctcagcccc
7441 agcaactaca gtctgggatg ccaaggccag ccatgatgtc agtggcccag catggtcaac
7501 ctttgaacat ggctccacaa ccaggattgg gccaggtagg tatcagccca ctcaaaccag
7561 gcactgtgtc tcaacaagcc ttacaaaacc ttttgcggac tctcaggtct cccagctctc
7621 ccctgcagca gcaacaggtg cttagtatca ttcacgccaa cccccagctg ttggctgcat
7681 tcatcaagca gcgggctgcc aagtatgcca actctaatcc acaacccatc cctgggcagc
7741 ctggcatgcc ccaggggcag ccagggctac agccacctac catgccaggt cagcaggggg
7801 tccactccaa tccagccatg cagaacatga atccaatgca ggcgggcgtt cagagggctg
7861 gcctgcccca gcagcaacca cagcagcaac tccagccacc catgggaggg atgagccccc
7921 aggctcagca gatgaacatg aaccacaaca ccatgccttc acaattccga gacatcttga
7981 gacgacagca aatgatgcaa cagcagcagc aacagggagc agggccagga ataggccctg
8041 gaatggccaa ccataaccag ttccagcaac cccaaggagt tggctaccca ccacagcagc
8101 agcagcggat gcagcatcac atgcaacaga tgcaacaagg aaatatggga cagataggcc
8161 agcttcccca ggccttggga gcagaggcag gtgccagtct acaggcctat cagcagcgac
8221 tccttcagca acagatgggg tcccctgttc agcccaaccc catgagcccc cagcagcata
8281 tgctcccaaa tcaggcccag tccccacacc tacaaggcca gcagatccct aattctctct
8341 ccaatcaagt gcgctctccc cagcctgtcc cttctccacg gccacagtcc cagccccccc
8401 actccagtcc ttccccaagg atgcagcctc agccttctcc acaccacgtt tccccacaga
8461 caagttcccc acatcctgga ctggtagctg cccaggccaa ccccatggaa caagggcatt
8521 ttgccagccc ggaccagaat tcaatgcttt ctcagcttgc tagcaatcca ggcatggcaa
8581 acctccatgg tgcaagcgcc acggacctgg gactcagcac cgataactca gacttgaatt
8641 caaacctctc acagagtaca ctagacatac acgaacaaaa acttatttct gaagaagacc
8701 tatagaagct tatgcataga tctatgcata gatctatgca tagatcctga gaacttcagg
8761 gtgagtttgg ggacccttga ttgttctttc tttttcgcta ttgtaaaatt catgttatat
8821 ggagggggca aagttttcag ggtgttgttt agaatgggaa gatgtccctt gtatcaccat
8881 ggaccctcat gataattttg tttctttcac tttctactct gttgacaacc attgtctcct
8941 cttattttct tttcattttc tgtaactttt tcgttaaact ttagcttgca tttgtaacga
9001 atttttaaat tcacttttgt ttatttgtca gattgtaagt actttctcta atcacttttt
9061 tttcaaggca atcagggtat attatattgt acttcagcac agttttagag aacaattgtt
9121 ataattaaat gataaggtag aatatttctg catataaatt ctggctggcg tggaaatatt
9181 cttattggta gaaacaacta caccctggtc atcatcctgc ctttctcttt atggttacaa
9241 tgatatacac tgtttgagat gaggataaaa tactctgagt ccaaaccggg cccctctgct
9301 aaccatgttc atgccttctt ctctttccta cagctcctgg gcaacgtgct ggttgttgtg
9361 ctgtctcatc attttggcaa agaattcact cctcaggtgc aggctgccta tcagaaggtg
9421 gtggctggtg tggccaatgc cctggctcac aaataccact gagatctttt tccctctgcc
9481 aaaaattatg gggacatcat gaagcccctt gagcatctga cttctggcta ataaaggaaa
9541 tttattttca ttgcaatagt gtgttggaat tttttgtgtc tctcactcgg aaggacatat
9601 gggagggcaa atcatttaaa acatcagaat gagtatttgg tttagagttt ggcaacatat
9661 gcccatatgc tggctgccat gaacaaaggt tggctataaa gaggtcatca gtatatgaaa
9721 cagccccctg ctgtccattc cttattccat agaaaagcct tgacttgagg ttagattttt
9781 tttatatttt gttttgtgtt atttttttct ttaacatccc taaaattttc cttacatgct
9841 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
9901 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
9961 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
10021 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
10081 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
10141 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
10201 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
10261 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
10321 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
10381 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
10441 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
10501 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
10561 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
10621 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
10681 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
10741 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
10801 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
10861 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
10921 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
10981 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
11041 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
11101 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
11161 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
11221 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
11281 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
11341 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
11401 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
11461 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
11521 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
11581 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
11641 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
11701 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
11761 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
11821 acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac
11881 gaggcccttt cgt
//
载体图谱质粒图谱pdf版和相关资料下载
载体质粒应用举例
- 上一篇：mEos3.2-Clathrin-15
- 下一篇：pCAGGS-HA-hUBE1L
