| 质粒名称: | pCR4-24XMS2SL-stable |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 31865 |
| 存储实验室: | Robert Singer |
|
载体骨架基本信息 |
|
| 载体名称: | pCR4Blunt-TOPO |
| 载体出品公司: | Invitrogen |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 3956 bp |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | -- |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | 24xMS2 Stem Loops - Stable |
| 物种来源: | -- |
| 插入大小: | 1300 bp |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | TOPO克隆 |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | M13F |
| 3' 测序引物 | M13R |
|
文献引用方法 |
|
| 材料和方法部分: | pCR4-24XMS2SL-stable was a gift from Robert Singer (Addgene plasmid # 31865 ; http://n2t.net/addgene:31865 ; RRID:Addgene_31865) |
| 参考文献部分: | Localization of ASH1 mRNA particles in living yeast. Bertrand E, Chartrand P, Schaefer M, Shenoy SM, Singer RH, Long RM. Mol Cell. 1998 Oct . 2(4):437-45. 10.1016/S1097-2765(00)80143-4 PubMed 9809065 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pCR4-24XMS2SL-stable 5256 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pCR4-24XMS2SL-stable
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5256)
AUTHORS Bertrand E, Chartrand P, Schaefer M, Shenoy SM, Singer RH, Long RM
TITLE Localization of ASH1 mRNA particles in living yeast.
JOURNAL Mol Cell. 1998 Oct . 2(4):437-45.
PUBMED 9809065
REFERENCE 2 (bases 1 to 5256)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..5256
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter complement(35..53)
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
primer_bind complement(74..90)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(74..90)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(87..109)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 98..114
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(122..152)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 167..188
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(305..322)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(476..1064)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(556..575)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS 1372..1750
/label=ISS
/note="immunostimulatory sequence from the AmpR gene;
contains unmethylated CpG dinucleotides in the context of
5'-AACGTT-3' (Sato et al., 1996)"
primer_bind complement(1406..1425)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
CDS complement(2296..3090)
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(2407..2426)
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
primer_bind 3017..3036
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
CDS complement(3439..78)
/codon_start=1
/gene="lacZ fragment"
/product="LacZ-alpha fragment of beta-galactosidase"
/label=lacZ-alpha
/translation="MTMITPSSELTLTKGTSPAGLNEFALRSDEPWNTGATRDRRRLKI
RPGGTADARAFLAISTVGSDEPWNTGATRDRRRLKIRPGGTADARAFLAISTVGSDEPW
NTGATRDRRRLKIRPGGTADARAFLAISTVGSDEPWNTGATRDRRRLKIRPGGTADARA
FLAISTVGSDEPWNTGATRDRRRLKIRPGGTADARAFLAISTVGSDEPWNTGATRDRRR
LKIRPGGTADARAFLAISTVGSDEPWNTGATRDRRRLKIRPGGTADARAFLAISTVGSD
EPWNTGATRDRRRLKIRPGGTADARAFLAISTVGSDEPWNTGATRDRRRLKIRPGGTAD
ARAFLAISTVGSDEPWNTGATRDRRRLKIRPGGTADARAFLAISTVGSDEPWNTGATRD
RRRLKIRPGGTADARAFLAISTVGSDEPWNTGATRDRRRLKIRPGGTADARAFLAISTV
GSKGEFAAAKFNSPYSESYYNSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEAR
TDRPSQQLRSLYVRQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKV
SRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
primer_bind complement(3541..3560)
/label=ccdB-fwd
/note="ccdB gene, forward primer"
primer_bind 3864..3886
/label=M13/pUC Forward
/note="In lacZ gene"
primer_bind 3878..3895
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind 3879..3895
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 3902..3921
/label=T7
/note="T7 promoter, forward primer"
promoter 3902..3920
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
1 aagggcgaat tcgtttaaac ctgcaggact agtcccttta gtgagggtta attctgagct
61 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
121 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
181 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
241 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
301 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
361 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
421 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
481 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
541 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
601 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
661 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
721 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
781 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
841 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
901 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
961 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
1021 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
1081 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
1141 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
1201 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
1261 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
1321 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
1381 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
1441 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
1501 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
1561 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
1621 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
1681 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
1741 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
1801 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gccttccggc
1861 tggctggttt attgctgata aatctggagc cggtgagcgt ggtctcgcgg tatcattgca
1921 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
1981 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
2041 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
2101 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgagcgg atacatattt
2161 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
2221 cctgatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggaaatt
2281 gtaagcgtta ataattcaga agaactcgtc aagaaggcga tagaaggcga tgcgctgcga
2341 atcgggagcg gcgataccgt aaagcacgag gaagcggtca gcccattcgc cgccaagctc
2401 ttcagcaata tcacgggtag ccaacgctat gtcctgatag cggtccgcca cacccagccg
2461 gccacagtcg atgaatccag aaaagcggcc attttccacc atgatattcg gcaagcaggc
2521 atcgccatgg gtcacgacga gatcctcgcc gtcgggcatg ctcgccttga gcctggcgaa
2581 cagttcggct ggcgcgagcc cctgatgctc ttcgtccaga tcatcctgat cgacaagacc
2641 ggcttccatc cgagtacgtg ctcgctcgat gcgatgtttc gcttggtggt cgaatgggca
2701 ggtagccgga tcaagcgtat gcagccgccg cattgcatca gccatgatgg atactttctc
2761 ggcaggagca aggtgagatg acaggagatc ctgccccggc acttcgccca atagcagcca
2821 gtcccttccc gcttcagtga caacgtcgag cacagctgcg caaggaacgc ccgtcgtggc
2881 cagccacgat agccgcgctg cctcgtcttg cagttcattc agggcaccgg acaggtcggt
2941 cttgacaaaa agaaccgggc gcccctgcgc tgacagccgg aacacggcgg catcagagca
3001 gccgattgtc tgttgtgccc agtcatagcc gaatagcctc tccacccaag cggccggaga
3061 acctgcgtgc aatccatctt gttcaatcat gcgaaacgat cctcatcctg tctcttgatc
3121 agagcttgat cccctgcgcc atcagatcct tggcggcaag aaagccatcc agtttacttt
3181 gcagggcttc ccaaccttac cagagggcgc cccagctggc aattccggtt cgcttgctgt
3241 ccataaaacc gcccagtcta gctatcgcca tgtaagccca ctgcaagcta cctgctttct
3301 ctttgcgctt gcgttttccc ttgtccagat agcccagtag ctgacattca tccggggtca
3361 gcaccgtttc tgcggactgg ctttctacgt gaaaaggatc taggtgaaga tcctttttga
3421 taatctcatg cctgacattt atattcccca gaacatcagg ttaatggcgt ttttgatgtc
3481 attttcgcgg tggctgagat cagccacttc ttccccgata acggagaccg gcacactggc
3541 catatcggtg gtcatcatgc gccagctttc atccccgata tgcaccaccg ggtaaagttc
3601 acgggagact ttatctgaca gcagacgtgc actggccagg gggatcacca tccgtcgccc
3661 cggcgtgtca ataatatcac tctgtacatc cacaaacaga cgataacggc tctctctttt
3721 ataggtgtaa accttaaact gccgtacgta taggctgcgc aactgttggg aagggcgatc
3781 ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt
3841 aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt
3901 gtaatacgac tcactatagg gcgaattgaa tttagcggcc gcgaattcgc ccttggatcc
3961 tacggtactt attgccaaga aagcacgagc atcagccgtg cctccaggtc gaatcttcaa
4021 acgacgacga tcacgcgtcg ctccagtatt ccagggttca tcagatccta cggtacttat
4081 tgccaagaaa gcacgagcat cagccgtgcc tccaggtcga atcttcaaac gacgacgatc
4141 acgcgtcgct ccagtattcc agggttcatc agatcctacg gtacttattg ccaagaaagc
4201 acgagcatca gccgtgcctc caggtcgaat cttcaaacga cgacgatcac gcgtcgctcc
4261 agtattccag ggttcatcag atcctacggt acttattgcc aagaaagcac gagcatcagc
4321 cgtgcctcca ggtcgaatct tcaaacgacg acgatcacgc gtcgctccag tattccaggg
4381 ttcatcagat cctacggtac ttattgccaa gaaagcacga gcatcagccg tgcctccagg
4441 tcgaatcttc aaacgacgac gatcacgcgt cgctccagta ttccagggtt catcagatcc
4501 tacggtactt attgccaaga aagcacgagc atcagccgtg cctccaggtc gaatcttcaa
4561 acgacgacga tcacgcgtcg ctccagtatt ccagggttca tcagatccta cggtacttat
4621 tgccaagaaa gcacgagcat cagccgtgcc tccaggtcga atcttcaaac gacgacgatc
4681 acgcgtcgct ccagtattcc agggttcatc agatcctacg gtacttattg ccaagaaagc
4741 acgagcatca gccgtgcctc caggtcgaat cttcaaacga cgacgatcac gcgtcgctcc
4801 agtattccag ggttcatcag atcctacggt acttattgcc aagaaagcac gagcatcagc
4861 cgtgcctcca ggtcgaatct tcaaacgacg acgatcacgc gtcgctccag tattccaggg
4921 ttcatcagat cctacggtac ttattgccaa gaaagcacga gcatcagccg tgcctccagg
4981 tcgaatcttc aaacgacgac gatcacgcgt cgctccagta ttccagggtt catcagatcc
5041 tacggtactt attgccaaga aagcacgagc atcagccgtg cctccaggtc gaatcttcaa
5101 acgacgacga tcacgcgtcg ctccagtatt ccagggttca tcagatccta cggtacttat
5161 tgccaagaaa gcacgagcat cagccgtgcc tccaggtcga atcttcaaac gacgacgatc
5221 acgcgtcgct ccagtattcc agggttcatc agatct
//
