| 质粒名称: | pCRY2PHR-mCherryN1 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 26866 |
| 存储实验室: | Chandra Tucker |
|
载体骨架基本信息 |
|
| 载体名称: | pmCherryN1 |
| 载体出品公司: | Clontech |
| 载体抗性: | 卡那霉素 |
| 空载体大小: | 4722 bp |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | CRY2PHR-mCh,cryptochrome 2,mCherry |
| 物种来源: | A. thaliana (mustard weed) |
| 插入大小: | 1507 bp |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | mCherry（主干上的C终端） |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | Xho I (未破坏) |
| 3' 克隆位点: | Xho I (未破坏) |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pCRY2PHR-mCherryN1 was a gift from Chandra Tucker (Addgene plasmid # 26866 ; http://n2t.net/addgene:26866 ; RRID:Addgene_26866) |
| 参考文献部分: | Rapid blue-light-mediated induction of protein interactions in living cells. Kennedy MJ, Hughes RM, Peteya LA, Schwartz JW, Ehlers MD, Tucker CL. Nat Methods. 2010 Oct 31. ():. 10.1038/nmeth.1524 PubMed 21037589 |
买家导航
pCRY2PHRmCherryN1载体基本信息
pCRY2PHRmCherryN1载体图谱质粒图谱和多克隆位点信息
pCRY2PHRmCherryN1载体简介
pCRY2PHRmCherryN1载体序列
LOCUS pCRY2PHR-mCherryN1 6188 bp ds-DNA circular SYN 23-APR-2020
DEFINITION Expresses CRY2(1-498)-mCherry fusion for use in light-inducible
protein interaction modules.
ACCESSION .
VERSION .
KEYWORDS pCRY2PHR-mCherryN1
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6188)
AUTHORS Kennedy MJ, Hughes RM, Peteya LA, Schwartz JW, Ehlers MD, Tucker CL
TITLE Rapid blue-light-mediated induction of protein interactions in
living cells.
JOURNAL Nat Methods. 2010 Oct 31. ():.
PUBMED 21037589
REFERENCE 2 (bases 1 to 6188)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Apr 23, 2020 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..6188
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 61..364
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 365..568
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 519..539
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
regulatory 2137..2146
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 2143..2853
/codon_start=1
/product="monomeric derivative of DsRed fluorescent protein
(Shaner et al., 2004)"
/label=mCherry
/note="mammalian codon-optimized"
/translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
EGRHSTGGMDELYK"
primer_bind complement(2288..2306)
/label=mCherry-R
/note="mCherry, reverse primer"
primer_bind 2556..2575
/label=mCherry-F
/note="mCherry, forward primer"
polyA_signal 2976..3097
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(3013..3032)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 3067..3086
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
rep_origin complement(3104..3559)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(3241..3262)
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind 3453..3472
/label=F1ori-R
/note="F1 origin, reverse primer"
promoter 3586..3690
/gene="bla"
/label=AmpR promoter
promoter 3692..4049
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
primer_bind complement(3715..3735)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
rep_origin 3900..4035
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 3962..3981
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
CDS 4084..4878
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(4138..4157)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 4748..4767
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
primer_bind complement(5066..5085)
/label=TK-pA-R
/note="Thymidine kinase polyA, reverse primer"
polyA_signal 5110..5157
/label=HSV TK poly(A) signal
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
rep_origin 5486..6074
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 5975..5994
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
ORIGIN
1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
601 ccggactcag atctcgaggc caccatgaag atggacaaaa agactatagt ttggtttaga
661 agagacctaa ggattgagga taatcctgca ttagcagcag ctgctcacga aggatctgtt
721 tttcctgtct tcatttggtg tcctgaagaa gaaggacagt tttatcctgg aagagcttca
781 agatggtgga tgaaacaatc acttgctcac ttatctcaat ccttgaaggc tcttggatct
841 gacctcactt taatcaaaac ccacaacacg atttcagcga tcttggattg tatccgcgtt
901 accggtgcta caaaagtcgt ctttaaccac ctctatgatc ctgtttcgtt agttcgggac
961 cataccgtaa aggagaagct ggtggaacgt gggatctctg tgcaaagcta caatggagat
1021 ctattgtatg aaccgtggga gatatactgc gaaaagggca aaccttttac gagtttcaat
1081 tcttactgga agaaatgctt agatatgtcg attgaatccg ttatgcttcc tcctccttgg
1141 cggttgatgc caataactgc agcggctgaa gcgatttggg cgtgttcgat tgaagaacta
1201 gggctggaga atgaggccga gaaaccgagc aatgcgttgt taactagagc ttggtctcca
1261 ggatggagca atgctgataa gttactaaat gagttcatcg agaagcagtt gatagattat
1321 gcaaagaaca gcaagaaagt tgttgggaat tctacttcac tactttctcc gtatctccat
1381 ttcggggaaa taagcgtcag acacgttttc cagtgtgccc ggatgaaaca aattatatgg
1441 gcaagagata agaacagtga aggagaagaa agtgcagatc tttttcttag gggaatcggt
1501 ttaagagagt attctcggta tatatgtttc aacttcccgt ttactcacga gcaatcgttg
1561 ttgagtcatc ttcggttttt cccttgggat gctgatgttg ataagttcaa ggcctggaga
1621 caaggcagga ccggttatcc gttggtggat gccggaatga gagagctttg ggctaccgga
1681 tggatgcata acagaataag agtgattgtt tcaagctttg ctgtgaagtt tcttctcctt
1741 ccatggaaat ggggaatgaa gtatttctgg gatacacttt tggatgctga tttggaatgt
1801 gacatccttg gctggcagta tatctctggg agtatccccg atggccacga gcttgatcgc
1861 ttggacaatc ccgcgttaca aggcgccaaa tatgacccag aaggtgagta cataaggcaa
1921 tggcttcccg agcttgcgag attgccaact gaatggatcc atcatccatg ggacgctcct
1981 ttaaccgtac tcaaagcttc tggtgtggaa ctcggaacaa actatgcgaa acccattgta
2041 gacatcgaca cagctcgtga gctactagct aaagctattt caagaacccg tgaagcacag
2101 atcatgatcg gagcagcagc ccgggatcca ccggtcgcca ccatggtgag caagggcgag
2161 gaggataaca tggccatcat caaggagttc atgcgcttca aggtgcacat ggagggctcc
2221 gtgaacggcc acgagttcga gatcgagggc gagggcgagg gccgccccta cgagggcacc
2281 cagaccgcca agctgaaggt gaccaagggt ggccccctgc ccttcgcctg ggacatcctg
2341 tcccctcagt tcatgtacgg ctccaaggcc tacgtgaagc accccgccga catccccgac
2401 tacttgaagc tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac
2461 ggcggcgtgg tgaccgtgac ccaggactcc tccctgcagg acggcgagtt catctacaag
2521 gtgaagctgc gcggcaccaa cttcccctcc gacggccccg taatgcagaa gaagaccatg
2581 ggctgggagg cctcctccga gcggatgtac cccgaggacg gcgccctgaa gggcgagatc
2641 aagcagaggc tgaagctgaa ggacggcggc cactacgacg ctgaggtcaa gaccacctac
2701 aaggccaaga agcccgtgca gctgcccggc gcctacaacg tcaacatcaa gttggacatc
2761 acctcccaca acgaggacta caccatcgtg gaacagtacg aacgcgccga gggccgccac
2821 tccaccggcg gcatggacga gctgtacaag taaagcggcc gcgactctag atcataatca
2881 gccataccac atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga
2941 acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg
3001 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
3061 ctagttgtgg tttgtccaaa ctcatcaatg tatcttaagg cgtaaattgt aagcgttaat
3121 attttgttaa aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc
3181 gaaatcggca aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt
3241 ccagtttgga acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa
3301 accgtctatc agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg
3361 tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga
3421 cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct
3481 agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat
3541 gcgccgctac agggcgcgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
3601 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
3661 atgcttcaat aatattgaaa aaggaagagt cctgaggcgg aaagaaccag ctgtggaatg
3721 tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
3781 tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa
3841 gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta actccgccca
3901 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
3961 ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag
4021 gcttttttgg aggcctaggc ttttgcaaag atcgatcaag agacaggatg aggatcgttt
4081 cgcatgattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta
4141 ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg
4201 tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa
4261 ctgcaagacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct
4321 gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg
4381 caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca
4441 atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat
4501 cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac
4561 gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gagcatgccc
4621 gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa
4681 aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag
4741 gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc
4801 ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt
4861 cttgacgagt tcttctgagc gggactctgg ggttcgaaat gaccgaccaa gcgacgccca
4921 acctgccatc acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa
4981 tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct
5041 tcgcccaccc tagggggagg ctaactgaaa cacggaagga gacaataccg gaaggaaccc
5101 gcgctatgac ggcaataaaa agacagaata aaacgcacgg tgttgggtcg tttgttcata
5161 aacgcggggt tcggtcccag ggctggcact ctgtcgatac cccaccgaga ccccattggg
5221 gccaatacgc ccgcgtttct tccttttccc caccccaccc cccaagttcg ggtgaaggcc
5281 cagggctcgc agccaacgtc ggggcggcag gccctgccat agcctcaggt tactcatata
5341 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
5401 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
5461 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
5521 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
5581 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
5641 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
5701 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
5761 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
5821 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
5881 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
5941 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
6001 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
6061 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
6121 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
6181 ccatgcat
//
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