pCVA

LOCUS       pCVA               14215 bp ds-DNA     circular SYN 13-MAY-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pCVA
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14215)
  AUTHORS   Tang Y, Wang F, Zhao J, Xie K, Hong Y, Liu Y
  TITLE     Virus-based microRNA expression for gene functional analysis in 
            plants.
  JOURNAL   Plant Physiol. 2010 Jun;153(2):632-41. Epub 2010 Apr 13.
  PUBMED    20388670
REFERENCE   2  (bases 1 to 14215)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 13, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..14215
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     intron          10..199
                     /label=cat1 intron
                     /note="castor bean catalase intron, modified"
     CDS             2024..2041
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      2076..2328
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    2350..2374
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             3674..4303
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             4737..5804
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     rep_origin      5870..6064
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(6300..6322)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    6408..6548
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     6463..6482
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(6563..6580)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6734..7322)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6814..6833)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(7409..8203)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    8628..8652
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    8730..8904
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(8944..9969)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     promoter        complement(10037..10714)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     protein_bind    10905..10926
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        10941..10971
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10979..10995
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10984..11006
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11003..11019
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11003..11019
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(13455..13472)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(13455..13471)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(13464..13486)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     promoter        13848..14193
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     14136..14156
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             join(14210..9,200..2017)
                     /codon_start=1
                     /product="beta-glucuronidase"
                     /label=GUS
                     /note="This version of the gusA gene has a 5' extension 
                     with a catalase intron to ensure expression in plants but 
                     not bacteria."
                     /translation="MVDLRNRRLVRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWES
                     ALQESRAIAVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGK
                     VWVNNQEVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGK
                     KKQSYFHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSV
                     ELRDADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELEVTAKSQTESDIYPLRVGIRS
                     VAVKGQQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYP
                     YAEEMLDWADEHGIVVIDETAAVGFQLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQA
                     IKELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVDT
                     LAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGNKK
                     GIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
ORIGIN
        1 gatctgaggg taaatttcta gtttttctcc ttcattttct tggttaggac ccttttctct
       61 ttttattttt ttgagctttg atctttcttt aaactgatct attttttaat tgattggtta
      121 tggtgtaaat attacatagc tttaactgat aatctgatta ctttatttcg tgtgtctatg
      181 atgatgatga tagttacaga accgacgact cgtccgtcct gtagaaaccc caacccgtga
      241 aatcaaaaaa ctcgacggcc tgtgggcatt cagtctggat cgcgaaaact gtggaattga
      301 tcagcgttgg tgggaaagcg cgttacaaga aagccgggca attgctgtgc caggcagttt
      361 taacgatcag ttcgccgatg cagatattcg taattatgcg ggcaacgtct ggtatcagcg
      421 cgaagtcttt ataccgaaag gttgggcagg ccagcgtatc gtgctgcgtt tcgatgcggt
      481 cactcattac ggcaaagtgt gggtcaataa tcaggaagtg atggagcatc agggcggcta
      541 tacgccattt gaagccgatg tcacgccgta tgttattgcc gggaaaagtg tacgtatcac
      601 cgtttgtgtg aacaacgaac tgaactggca gactatcccg ccgggaatgg tgattaccga
      661 cgaaaacggc aagaaaaagc agtcttactt ccatgatttc tttaactatg ccggaatcca
      721 tcgcagcgta atgctctaca ccacgccgaa cacctgggtg gacgatatca ccgtggtgac
      781 gcatgtcgcg caagactgta accacgcgtc tgttgactgg caggtggtgg ccaatggtga
      841 tgtcagcgtt gaactgcgtg atgcggatca acaggtggtt gcaactggac aaggcactag
      901 cgggactttg caagtggtga atccgcacct ctggcaaccg ggtgaaggtt atctctatga
      961 actcgaagtc acagccaaaa gccagacaga gtctgatatc tacccgcttc gcgtcggcat
     1021 ccggtcagtg gcagtgaagg gccaacagtt cctgattaac cacaaaccgt tctactttac
     1081 tggctttggt cgtcatgaag atgcggactt acgtggcaaa ggattcgata acgtgctgat
     1141 ggtgcacgac cacgcattaa tggactggat tggggccaac tcctaccgta cctcgcatta
     1201 cccttacgct gaagagatgc tcgactgggc agatgaacat ggcatcgtgg tgattgatga
     1261 aactgctgct gtcggctttc agctgtcttt aggcattggt ttcgaagcgg gcaacaagcc
     1321 gaaagaactg tacagcgaag aggcagtcaa cggggaaact cagcaagcgc acttacaggc
     1381 gattaaagag ctgatagcgc gtgacaaaaa ccacccaagc gtggtgatgt ggagtattgc
     1441 caacgaaccg gatacccgtc cgcaaggtgc acgggaatat ttcgcgccac tggcggaagc
     1501 aacgcgtaaa ctcgacccga cgcgtccgat cacctgcgtc aatgtaatgt tctgcgacgc
     1561 tcacaccgat accatcagcg atctctttga tgtgctgtgc ctgaaccgtt attacggatg
     1621 gtatgtccaa agcggcgatt tggaaacggc agagaaggta ctggaaaaag aacttctggc
     1681 ctggcaggag aaactgcatc agccgattat catcaccgaa tacggcgtgg atacgttagc
     1741 cgggctgcac tcaatgtaca ccgacatgtg gagtgaagag tatcagtgtg catggctgga
     1801 tatgtatcac cgcgtctttg atcgcgtcag cgccgtcgtc ggtgaacagg tatggaattt
     1861 cgccgatttt gcgacctcgc aaggcatatt gcgcgttggc ggtaacaaga aagggatctt
     1921 cactcgcgac cgcaaaccga agtcggcggc ttttctgctg caaaaacgct ggactggcat
     1981 gaacttcggt gaaaaaccgc agcagggagg caaacaagct agccaccacc accaccacca
     2041 cgtgtgaatt acaggtgacc agctcgaatt tccccgatcg ttcaaacatt tggcaataaa
     2101 gtttcttaag attgaatcct gttgccggtc ttgcgatgat tatcatataa tttctgttga
     2161 attacgttaa gcatgtaata attaacatgt aatgcatgac gttatttatg agatgggttt
     2221 ttatgattag agtcccgcaa ttatacattt aatacgcgat agaaaacaaa atatagcgcg
     2281 caaactagga taaattatcg cgcgcggtgt catctatgtt actagatcgg gaattaaact
     2341 atcagtgttt gacaggatat attggcgggt aaacctaaga gaaaagagcg tttattagaa
     2401 taacggatat ttaaaagggc gtgaaaaggt ttatccgttc gtccatttgt atgtgcatgc
     2461 caaccacagg gttcccctcg ggatcaaagt actttgatcc aacccctccg ctgctatagt
     2521 gcagtcggct tctgacgttc agtgcagccg tcttctgaaa acgacatgtc gcacaagtcc
     2581 taagttacgc gacaggctgc cgccctgccc ttttcctggc gttttcttgt cgcgtgtttt
     2641 agtcgcataa agtagaatac ttgcgactag aaccggagac attacgccat gaacaagagc
     2701 gccgccgctg gcctgctggg ctatgcccgc gtcagcaccg acgaccagga cttgaccaac
     2761 caacgggccg aactgcacgc ggccggctgc accaagctgt tttccgagaa gatcaccggc
     2821 accaggcgcg accgcccgga gctggccagg atgcttgacc acctacgccc tggcgacgtt
     2881 gtgacagtga ccaggctaga ccgcctggcc cgcagcaccc gcgacctact ggacattgcc
     2941 gagcgcatcc aggaggccgg cgcgggcctg cgtagcctgg cagagccgtg ggccgacacc
     3001 accacgccgg ccggccgcat ggtgttgacc gtgttcgccg gcattgccga gttcgagcgt
     3061 tccctaatca tcgaccgcac ccggagcggg cgcgaggccg ccaaggcccg aggcgtgaag
     3121 tttggccccc gccctaccct caccccggca cagatcgcgc acgcccgcga gctgatcgac
     3181 caggaaggcc gcaccgtgaa agaggcggct gcactgcttg gcgtgcatcg ctcgaccctg
     3241 taccgcgcac ttgagcgcag cgaggaagtg acgcccaccg aggccaggcg gcgcggtgcc
     3301 ttccgtgagg acgcattgac cgaggccgac gccctggcgg ccgccgagaa tgaacgccaa
     3361 gaggaacaag catgaaaccg caccaggacg gccaggacga accgtttttc attaccgaag
     3421 agatcgaggc ggagatgatc gcggccgggt acgtgttcga gccgcccgcg cacgtctcaa
     3481 ccgtgcggct gcatgaaatc ctggccggtt tgtctgatgc caagctggcg gcctggccgg
     3541 ccagcttggc cgctgaagaa accgagcgcc gccgtctaaa aaggtgatgt gtatttgagt
     3601 aaaacagctt gcgtcatgcg gtcgctgcgt atatgatgcg atgagtaaat aaacaaatac
     3661 gcaaggggaa cgcatgaagg ttatcgctgt acttaaccag aaaggcgggt caggcaagac
     3721 gaccatcgca acccatctag cccgcgccct gcaactcgcc ggggccgatg ttctgttagt
     3781 cgattccgat ccccagggca gtgcccgcga ttgggcggcc gtgcgggaag atcaaccgct
     3841 aaccgttgtc ggcatcgacc gcccgacgat tgaccgcgac gtgaaggcca tcggccggcg
     3901 cgacttcgta gtgatcgacg gagcgcccca ggcggcggac ttggctgtgt ccgcgatcaa
     3961 ggcagccgac ttcgtgctga ttccggtgca gccaagccct tacgacatat gggccaccgc
     4021 cgacctggtg gagctggtta agcagcgcat tgaggtcacg gatggaaggc tacaagcggc
     4081 ctttgtcgtg tcgcgggcga tcaaaggcac gcgcatcggc ggtgaggttg ccgaggcgct
     4141 ggccgggtac gagctgccca ttcttgagtc ccgtatcacg cagcgcgtga gctacccagg
     4201 cactgccgcc gccggcacaa ccgttcttga atcagaaccc gagggcgacg ctgcccgcga
     4261 ggtccaggcg ctggccgctg aaattaaatc aaaactcatt tgagttaatg aggtaaagag
     4321 aaaatgagca aaagcacaaa cacgctaagt gccggccgtc cgagcgcacg cagcagcaag
     4381 gctgcaacgt tggccagcct ggcagacacg ccagccatga agcgggtcaa ctttcagttg
     4441 ccggcggagg atcacaccaa gctgaagatg tacgcggtac gccaaggcaa gaccattacc
     4501 gagctgctat ctgaatacat cgcgcagcta ccagagtaaa tgagcaaatg aataaatgag
     4561 tagatgaatt ttagcggcta aaggaggcgg catggaaaat caagaacaac caggcaccga
     4621 cgccgtggaa tgccccatgt gtggaggaac gggcggttgg ccaggcgtaa gcggctgggt
     4681 tgtctgccgg ccctgcaatg gcactggaac ccccaagccc gaggaatcgg cgtgacggtc
     4741 gcaaaccatc cggcccggta caaatcggcg cggcgctggg tgatgacctg gtggagaagt
     4801 tgaaggccgc gcaggccgcc cagcggcaac gcatcgaggc agaagcacgc cccggtgaat
     4861 cgtggcaagc ggccgctgat cgaatccgca aagaatcccg gcaaccgccg gcagccggtg
     4921 cgccgtcgat taggaagccg cccaagggcg acgagcaacc agattttttc gttccgatgc
     4981 tctatgacgt gggcacccgc gatagtcgca gcatcatgga cgtggccgtt ttccgtctgt
     5041 cgaagcgtga ccgacgagct ggcgaggtga tccgctacga gcttccagac gggcacgtag
     5101 aggtttccgc agggccggcc ggcatggcca gtgtgtggga ttacgacctg gtactgatgg
     5161 cggtttccca tctaaccgaa tccatgaacc gataccggga agggaaggga gacaagcccg
     5221 gccgcgtgtt ccgtccacac gttgcggacg tactcaagtt ctgccggcga gccgatggcg
     5281 gaaagcagaa agacgacctg gtagaaacct gcattcggtt aaacaccacg cacgttgcca
     5341 tgcagcgtac gaagaaggcc aagaacggcc gcctggtgac ggtatccgag ggtgaagcct
     5401 tgattagccg ctacaagatc gtaaagagcg aaaccgggcg gccggagtac atcgagatcg
     5461 agctagctga ttggatgtac cgcgagatca cagaaggcaa gaacccggac gtgctgacgg
     5521 ttcaccccga ttactttttg atcgatcccg gcatcggccg ttttctctac cgcctggcac
     5581 gccgcgccgc aggcaaggca gaagccagat ggttgttcaa gacgatctac gaacgcagtg
     5641 gcagcgccgg agagttcaag aagttctgtt tcaccgtgcg caagctgatc gggtcaaatg
     5701 acctgccgga gtacgatttg aaggaggagg cggggcaggc tggcccgatc ctagtcatgc
     5761 gctaccgcaa cctgatcgag ggcgaagcat ccgccggttc ctaatgtacg gagcagatgc
     5821 tagggcaaat tgccctagca ggggaaaaag gtcgaaaagg tctctttcct gtggatagca
     5881 cgtacattgg gaacccaaag ccgtacattg ggaaccggaa cccgtacatt gggaacccaa
     5941 agccgtacat tgggaaccgg tcacacatgt aagtgactga tataaaagag aaaaaaggcg
     6001 atttttccgc ctaaaactct ttaaaactta ttaaaactct taaaacccgc ctggcctgtg
     6061 cataactgtc tggccagcgc acagccgaag agctgcaaaa agcgcctacc cttcggtcgc
     6121 tgcgctccct acgccccgcc gcttcgcgtc ggcctatcgc ggccgctggc cgctcaaaaa
     6181 tggctggcct acggccaggc aatctaccag ggcgcggaca agccgcgccg tcgccactcg
     6241 accgccggcg cccacatcaa ggcaccctgc ctcgcgcgtt tcggtgatga cggtgaaaac
     6301 ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc
     6361 agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc agccatgacc
     6421 cagtcacgta gcgatagcgg agtgtatact ggcttaacta tgcggcatca gagcagattg
     6481 tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc
     6541 gcatcaggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     6601 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     6661 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     6721 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     6781 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     6841 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     6901 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     6961 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     7021 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     7081 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     7141 tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc
     7201 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     7261 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     7321 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     7381 aagggatttt ggtcatgcat tctaggtact aaaacaattc atccagtaaa atataatatt
     7441 ttattttctc ccaatcaggc ttgatcccca gtaagtcaaa aaatagctcg acatactgtt
     7501 cttccccgat atcctccctg atcgaccgga cgcagaaggc aatgtcatac cacttgtccg
     7561 ccctgccgct tctcccaaga tcaataaagc cacttacttt gccatctttc acaaagatgt
     7621 tgctgtctcc caggtcgccg tgggaaaaga caagttcctc ttcgggcttt tccgtcttta
     7681 aaaaatcata cagctcgcgc ggatctttaa atggagtgtc ttcttcccag ttttcgcaat
     7741 ccacatcggc cagatcgtta ttcagtaagt aatccaattc ggctaagcgg ctgtctaagc
     7801 tattcgtata gggacaatcc gatatgtcga tggagtgaaa gagcctgatg cactccgcat
     7861 acagctcgat aatcttttca gggctttgtt catcttcata ctcttccgag caaaggacgc
     7921 catcggcctc actcatgagc agattgctcc agccatcatg ccgttcaaag tgcaggacct
     7981 ttggaacagg cagctttcct tccagccata gcatcatgtc cttttcccgt tccacatcat
     8041 aggtggtccc tttataccgg ctgtccgtca tttttaaata taggttttca ttttctccca
     8101 ccagcttata taccttagca ggagacattc cttccgtatc ttttacgcag cggtattttt
     8161 cgatcagttt tttcaattcc ggtgatattc tcattttagc catttattat ttccttcctc
     8221 ttttctacag tatttaaaga taccccaaga agctaattat aacaagacga actccaattc
     8281 actgttcctt gcattctaaa accttaaata ccagaaaaca gctttttcaa agttgttttc
     8341 aaagttggcg tataacatag tatcgacgga gccgattttg aaaccgcggt gatcacaggc
     8401 agcaacgctc tgtcatcgtt acaatcaaca tgctaccctc cgcgagatca tccgtgtttc
     8461 aaacccggca gcttagttgc cgttcttccg aatagcatcg gtaacatgag caaagtctgc
     8521 cgccttacaa cggctctccc gctgacgccg tcccggactg atgggctgcc tgtatcgagt
     8581 ggtgattttg tgccgagctg ccggtcgggg agctgttggc tggctggtgg caggatatat
     8641 tgtggtgtaa acaaattgac gcttagacaa cttaataaca cattgcggac gtttttaatg
     8701 tactgaatta acgccgaatt aattcggggg atctggattt tagtactgga ttttggtttt
     8761 aggaattaga aattttattg atagaagtat tttacaaata caaatacata ctaagggttt
     8821 cttatatgct caacacatga gcgaaaccct ataggaaccc taattccctt atctgggaac
     8881 tactcacaca ttattatgga gaaactcgag cttgtcgatc gacagatccg gtcggcatct
     8941 actctatttc tttgccctcg gacgagtgct ggggcgtcgg tttccactat cggcgagtac
     9001 ttctacacag ccatcggtcc agacggccgc gcttctgcgg gcgatttgtg tacgcccgac
     9061 agtcccggct ccggatcgga cgattgcgtc gcatcgaccc tgcgcccaag ctgcatcatc
     9121 gaaattgccg tcaaccaagc tctgatagag ttggtcaaga ccaatgcgga gcatatacgc
     9181 ccggagtcgt ggcgatcctg caagctccgg atgcctccgc tcgaagtagc gcgtctgctg
     9241 ctccatacaa gccaaccacg gcctccagaa gaagatgttg gcgacctcgt attgggaatc
     9301 cccgaacatc gcctcgctcc agtcaatgac cgctgttatg cggccattgt ccgtcaggac
     9361 attgttggag ccgaaatccg cgtgcacgag gtgccggact tcggggcagt cctcggccca
     9421 aagcatcagc tcatcgagag cctgcgcgac ggacgcactg acggtgtcgt ccatcacagt
     9481 ttgccagtga tacacatggg gatcagcaat cgcgcatatg aaatcacgcc atgtagtgta
     9541 ttgaccgatt ccttgcggtc cgaatgggcc gaacccgctc gtctggctaa gatcggccgc
     9601 agcgatcgca tccatagcct ccgcgaccgg ttgtagaaca gcgggcagtt cggtttcagg
     9661 caggtcttgc aacgtgacac cctgtgcacg gcgggagatg caataggtca ggctctcgct
     9721 aaactcccca atgtcaagca cttccggaat cgggagcgcg gccgatgcaa agtgccgata
     9781 aacataacga tctttgtaga aaccatcggc gcagctattt acccgcagga catatccacg
     9841 ccctcctaca tcgaagctga aagcacgaga ttcttcgccc tccgagagct gcatcaggtc
     9901 ggagacgctg tcgaactttt cgatcagaaa cttctcgaca gacgtcgcgg tgagttcagg
     9961 ctttttcata tctcattgcc ccccgggatc tgcgaaagct cgagagagat agatttgtag
    10021 agagagactg gtgatttcag cgtgtcctct ccaaatgaaa tgaacttcct tatatagagg
    10081 aaggtcttgc gaaggatagt gggattgtgc gtcatccctt acgtcagtgg agatatcaca
    10141 tcaatccact tgctttgaag acgtggttgg aacgtcttct ttttccacga tgctcctcgt
    10201 gggtgggggt ccatctttgg gaccactgtc ggcagaggca tcttgaacga tagcctttcc
    10261 tttatcgcaa tgatggcatt tgtaggtgcc accttccttt tctactgtcc ttttgatgaa
    10321 gtgacagata gctgggcaat ggaatccgag gaggtttccc gatattaccc tttgttgaaa
    10381 agtctcaata gccctttggt cttctgagac tgtatctttg atattcttgg agtagacgag
    10441 agtgtcgtgc tccaccatgt tatcacatca atccacttgc tttgaagacg tggttggaac
    10501 gtcttctttt tccacgatgc tcctcgtggg tgggggtcca tctttgggac cactgtcggc
    10561 agaggcatct tgaacgatag cctttccttt atcgcaatga tggcatttgt aggtgccacc
    10621 ttccttttct actgtccttt tgatgaagtg acagatagct gggcaatgga atccgaggag
    10681 gtttcccgat attacccttt gttgaaaagt ctcaatagcc ctttggtctt ctgagactgt
    10741 atctttgata ttcttggagt agacgagagt gtcgtgctcc accatgttgg caagctgctc
    10801 tagccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac
    10861 gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc
    10921 actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt
    10981 gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacga attcgaggtc
    11041 gacatagaac gccacggggc atcccgctta ggcatttcga agtaaagcat aaattgaaat
    11101 cacatgtctt tatataggcc cacagcgcac tcagttagcc ttaatagcta accttgtcag
    11161 gcggcatgtg attggttaga tttcatttaa tgcgtggtcc cacgcaagac accaaaaaaa
    11221 tcgcggccat ccggtaatat tattgcggat ggccgctttt gcccctggtg ttccccagag
    11281 gcttttagta tatattggac accaagacac caaaaacaat aggttttaga gagagctctc
    11341 ctggcgtcct cccctggagt cctaaatatt atttacaaaa atgccacgaa accctaaatc
    11401 gtttcgttta gcagcccgaa atatattctt aactcaccta aggtgcgaca tacccaaaga
    11461 tgaagctctt cagatgcttc aaaccctgtc gtggtcagtc gtcaaaccca catacatcag
    11521 agtcgcaaga gaggaacatt cagacggttc cccatttaca cactgtctca tccaactatc
    11581 aggaaagtcg aacatcaagg atgctagatt tttcgacatc actcacccca gaaggtctgc
    11641 caattttcac ccaaacattc aggcagccaa agacaccaat gccgtcaaga attacatcac
    11701 caaagatggt gattattgtg aatccgggca gtacaaggtg tctgggggta caaaggcaaa
    11761 taaagacgac gtctaccaca acgccgtcaa tgcgggatgt gtggaagagc gtctcgcaat
    11821 tataagggct ggagttccaa agacgttcat tgttagttat cataatgtta gagctaacat
    11881 agagcgactc tttactaagg ctccggaacc atgggctcct ccgtttcaac tctcctcctt
    11941 tactaacgtc ccggacgaga tgagttcatg ggcagatgac tattttggtc ggagtgccgc
    12001 tgcgcgggcg gaaagaccta ttagtatcat agttgaaggt gattcacgaa ccggcaagac
    12061 catgtgggcg cgtgctttag gaccacataa ttatttgagt gggcacctcg actttaattc
    12121 aaaggtcttt tcaaataatg cggagtataa cgtcattgat gacatagctc cgcattatct
    12181 aaagctaaag cactggaaag agcttattgg ggcccaaagg gactggcaat caaactgtaa
    12241 gtacggcaag ccagttcaaa ttaaaggtgg cataccctca atcgtgctgt gcaatccagg
    12301 agaggggagc agttatataa gtttcctcaa caaagaggaa aatgcatcac taagagcgtg
    12361 gactaccaaa aatgcaaaat tcatcactct tgaagccccc ctctatcaaa gcacagcaca
    12421 agattgctaa aaggagagct gtacgccgaa gacgcattga tttgaactgc gggtgttcta
    12481 tcttcctcca catcaattgc gcagataatg gattcacgca ccggggagag catcactgta
    12541 gctcaggcag agaattccgt ttttatctgg gaggttccaa atcccctata tttcaggata
    12601 caacgcgtag aggacccgtt gtacaccaga accaggatct accacatcca agtccggttc
    12661 aaccacaacc tacggaaagc attggatctc cgcaaagcct acttcaactt ccaagtctgg
    12721 acgacttcga tgagagcttc tgggccgaca tatttaagta gattcaaatg ccttgtaatg
    12781 tctcatttag ataacctagg tgttattggt attaatcatg taattagagc tgttcgtttc
    12841 gcaacggaca gatcctatgt aactcatgtt cacgagaatc atgtaataaa tttcaaaatt
    12901 tattaatttg ttatcgaatc gtaaaaatag atccgaattt ttaatgtcgc atacacagga
    12961 ttaggctagc gagctcagat ctggatcctc tagactcgag ggtaccgcta gccgcttagg
    13021 catttcgaag taaagcataa attgaaatca catgtcttta tataggccca cagcgcactc
    13081 agttagcctt aatagctaac cttgtcaggc ggcatgtgat tggttagatt tcatttaatg
    13141 cgtggtccca cgcaagacac caaaaaaatc gcggccatcc ggtaatatta ttgcggatgg
    13201 ccgcttttgc ccctggtgtt ccccagaggc ttttagtata tattggacac caagacacca
    13261 aaaacaatag gttttagaga gagctctcct ggcgtcctcc cctggagtcc taaatattat
    13321 ttacaaaaat gccacgaaac cctaaatcgt ttcgtttagc agcccgaaat atattcttaa
    13381 ctcacctaag gtgcgacata cccaaagatg aagcggggga tccactagag cggccgccag
    13441 tcgacaagct tggcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt
    13501 acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag
    13561 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg ctagagcagc
    13621 ttgagcttgg atcagattgt cgtttcccgc cttcagttta gcttcatgga gtcaaagatt
    13681 caaatagagg acctaacaga actcgccgta aagactggcg aacagttcat acagagtctc
    13741 ttacgactca atgacaagaa gaaaatcttc gtcaacatgg tggagcacga cacacttgtc
    13801 tactccaaaa atatcaaaga tacagtctca gaagaccaaa gggcaattga gacttttcaa
    13861 caaagggtaa tatccggaaa cctcctcgga ttccattgcc cagctatctg tcactttatt
    13921 gtgaagatag tggaaaagga aggtggctcc tacaaatgcc atcattgcga taaaggaaag
    13981 gccatcgttg aagatgcctc tgccgacagt ggtcccaaag atggaccccc acccacgagg
    14041 agcatcgtgg aaaaagaaga cgttccaacc acgtcttcaa agcaagtgga ttgatgtgat
    14101 atctccactg acgtaaggga tgacgcacaa tcccactatc cttcgcaaga cccttcctct
    14161 atataaggaa gttcatttca tttggagaga acacggggga ctcttgacca tggta
//