| 质粒名称: | pFC330 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 87842 |
| 存储实验室: | Uffe Mortensen |
|
载体骨架基本信息 |
|
| 载体名称: | custom |
| 载体抗性: | 氨苄青霉素 |
| 完整质粒大小: | 15764 bp |
| 载体修饰: | AMA1元素 |
| 载体类型: | 细菌表达，CRISPR；真菌表达 |
| 筛选标记: | pyrG |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | Cas9 |
| 物种来源: | Synthetic; synthetic gene, codon optimized for A. niger |
| 插入大小: | 4131 bp |
| GenBank编号 | KT031983 |
| 启动子 | Aspergillus nidulans tef1 promoter |
| 标签/融合蛋白: | SV40 NLS（C端子插入） |
| 5' 测序引物 | CTTCTCTGCTCAGCACCTCTACG |
|
文献引用方法 |
|
| 材料和方法部分: | pFC330 was a gift from Uffe Mortensen (Addgene plasmid # 87842 ; http://n2t.net/addgene:87842 ; RRID:Addgene_87842) |
| 参考文献部分: | A CRISPR-Cas9 System for Genetic Engineering of Filamentous Fungi. Nodvig CS, Nielsen JB, Kogle ME, Mortensen UH. PLoS One. 2015 Jul 15;10(7):e0133085. doi: 10.1371/journal.pone.0133085. eCollection 2015. PONE-D-15-11561 [pii] PubMed 26177455 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pFC330 15753 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION AMA1 plasmid with Aspergillus optimized Cas9 and pyrG selection
marker.
ACCESSION .
VERSION .
KEYWORDS pFC330
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 15753)
AUTHORS Nodvig CS, Nielsen JB, Kogle ME, Mortensen UH
TITLE A CRISPR-Cas9 System for Genetic Engineering of Filamentous Fungi.
JOURNAL PLoS One. 2015 Jul 15;10(7):e0133085. doi:
10.1371/journal.pone.0133085. eCollection 2015.
PUBMED 26177455
REFERENCE 2 (bases 1 to 15753)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..15753
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind 622..641
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(860..951)
/gene="bla"
/label=AmpR promoter
primer_bind 993..1012
/label=Kan-R
/note="Kanamycin resistance gene, reverse primer"
primer_bind 1018..1037
/label=pENTR-R
/note="pENTR vectors, reverse primer"
protein_bind 1244..1277
/label=FRT (minimal)
/bound_moiety="FLP recombinase from the Saccharomyces
cerevisiae 2u plasmid"
/note="supports FLP-mediated excision but not integration
(Turan and Bode, 2011)"
CDS 8010..12113
/codon_start=1
/product="Cas9 (Csn1) endonuclease from the Streptococcus
pyogenes Type II CRISPR/Cas system"
/label=Cas9
/note="generates RNA-guided double strand breaks in DNA"
/translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
DATLIHQSITGLYETRIDLSQLGGD"
CDS 12117..12137
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
protein_bind complement(14253..14286)
/label=FRT (minimal)
/bound_moiety="FLP recombinase from the Saccharomyces
cerevisiae 2u plasmid"
/note="supports FLP-mediated excision but not integration
(Turan and Bode, 2011)"
primer_bind complement(14756..14773)
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind complement(14756..14772)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(14871..14888)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(15042..15630)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(15122..15141)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(15752..859)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
ORIGIN
1 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
61 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
121 gcgctgcgat gataccgcga gaaccacgct caccggctcc ggatttatca gcaataaacc
181 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
241 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
301 ttgttgccat cgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
361 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
421 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
481 tggttatggc agcgctacat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
541 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
601 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
661 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
721 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
781 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
841 ggaaatgttg aatactcata ttcttccttt ttcaatatta ttgaagcatt tatcagggtt
901 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggtca
961 gtgttacaac caattaacca attctgaaca ttatcgcgag cccatttata cctgaatatg
1021 gctcataaca ccccttgttt gcctggcggc agtagcgcgg tggtcccacc tgaccccatg
1081 ccgaactcag aagtgaaacg ccgtagcgcc gatggtagtg tggggactcc ccatgcgaga
1141 gtagggaact gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg
1201 cccgggctaa ttatggggtg tcgcccttat tcgactctat agtgaagttc ctattctcta
1261 gaaagtatag gaacttctga agtggggatt taaatgcggc cgcgctgagg gtttaatcga
1321 cgaagcagct gacggccagt gccaagctta acgcgtaccg ggcccagtat atgttccgca
1381 gatgactgga gctctgccat acgtgccctc tcaagcacca tttgttccat ctacagagac
1441 tagtcaccaa ctagtctatc aagactcaca gggtacattg ctgagaccaa ctgaccagag
1501 gcagggtagc ggattgacgg ctccatctcc ttcacttaca aggtctattg aaagcccttt
1561 agcatcacca agcggagaat agattgttaa gcttattttt tgtatactgt tttgtgatag
1621 cacgaagttt ttccacggta tcttgttaaa aatatatatt tgtggcgggc ttacctacat
1681 caaattaata agagactaat tataaactaa acacacaagc aagctacttt agggtaaaag
1741 tttataaatg cttttgacgt ataaacgttg cttgtattta ttattacaat taaaggtgga
1801 tagaaaacct agagactagt tagaaactaa tctcaggttt gcgttaaact aaatcagagc
1861 ccgagaggtt aacagaacct agaaggggac tagatatccg ggtagggaaa caaaaaaaaa
1921 aancaagaca gccacatatt agggagacta gttagaagct agttccagga ctaggaaaat
1981 aaaagacaat gataccacag tctagttgac aactagatag attctagatt gaggccaaag
2041 tctctgagat ccaggttagt tgcaactaat actagttagt atctagtctc ctataactct
2101 gaagctagaa taacttacta ctattatcct caccactgtt cagctgcgca aacggagtga
2161 ttgcaaggtg ttcagagact agttattgac tagtcagtga ctagcaataa ctaacaaggt
2221 attaacctac catgtctgcc atcaccctgc acttcctcgg gctcagcagc cttttcctcc
2281 tcattttcat gctcattttc cttgtttaag actgtgacta gtcraagact agtccagaac
2341 cacaaaggag aaatgtctta ccactttctt cattgcttgt ctcttttgca ttatccatgt
2401 ctgcaactag ttagagtcta gttagtgact agtccgacga ggacttgctt gtctccggat
2461 tgttggagga actctccagg gcctcaagat ccacaacaga gccttctaga agactggtca
2521 ataactagtt ggtctttgtc tgagtctgac ttacgaggtt gcatactcgc tccctttgcc
2581 tcgtcaatcg atgagaaaaa gcgccaaaac tcgcaatatg gctttgaacc acacggtgct
2641 gagactagtt agaatctagt cccaaactag cttggatagc ttacctttgc cctttgcgtt
2701 gcgacaggtc ttgcagggta tggttccttt ctcaccagct gatttagctg ccttgctacc
2761 ctcacggcgg atctgcataa agagtggcta gaggttataa attagcactg atcctaggta
2821 cggggctgaa tgtaacttgc cyttcctttc tcatcgcgcg gcaagacagg cttgctcaaa
2881 ttcctaccag tcacaggggt atgcacggcg tacggaccac ttgaactagt cacagattag
2941 ttagcaacta gtctgcattg aatggctgta cttacgggcc ctcgccattg tcctgatcat
3001 ttccagcttc accctcgttg ctgcaaagta gttagtgact agtcaaggac tagttgaaat
3061 gggagaagaa actcacgaat tctcgacacc cttagtattg tggtccttgg acttggtgct
3121 gctatatatt agctaataca ctagttagac tcacrgaaac ttacgcagct cgcttgcgct
3181 tcttggtagg agtcggggtt gggagaacag tgccttcaaa caagccttca taccatgcta
3241 cttgactagt cagggactag tcaccaagta atctagatag gacttgcctt tggcctccat
3301 cagttccttc atagtgggag gtccattgtg caatgtaaac tccatgccgt gggagttctt
3361 gtccttcaag tgcttgacca atatgtttct gttggcagag ggaacctgtc aactagttaa
3421 taactagtca gaaactagta tagcagtaga ctcactgtac gcttgaggca tcccttcact
3481 cggcagtaga cttcatatgg atggatatca ggcacgccat tgtcgtcctg tggactagtc
3541 agtaactagg cttaaagcta gtcgggtcgg cttactatct tgaaatccgg cagcgtaagc
3601 tccccgtcct taactgcctc gagatagtga cagtactctg gggactttcg gagatcgtta
3661 tcgcgaatgc tcggcatact aatcgttgac tagtcttgga ctagtcccga gcaaaaagga
3721 ttggaggagg aggaggaagg tgagagtgag acaaagagcg aaataagagc ttcaaaggct
3781 atctctaagc agtatgaagg ttaagtatct agttcttgac tagatttaaa agagatttcg
3841 actagttatg tacctggagt ttggatatag gaatgtgttg tggtaacgaa atgtaagggg
3901 gaggaaagaa aaagtcggtc aagaggtaac tctaagtcgg ccattccttt ttgggaggcg
3961 ctaaccataa acggcatggt cgacttagag ttagctcagg gaatttaggg agttatctgc
4021 gaccaccgag gaacggcgga atgccaaaga atcccgatgg agctctagct ggcggttgac
4081 aaccccacct tttggcgttt ctgcggcgtt gcaggcggga ctggatactt cgtagaacca
4141 gaaaggcaag gcagaacgcg ctcagcaaga gtgttggaag tgatagcatg atgtgccttg
4201 ttaactaggt caaaatctgc agtatgcttg atgttatcca aagtgtgaga gaggaaggtc
4261 caaacataca cgattgggag agggcctagg tataagagtt tttgagtaga acgcatgtga
4321 gcccagccat ctcgaggaga ttaaacacgg gccggcattt gatggctatg ttagtacccc
4381 aatggaaacg gtgagagtcc agtggtcgca gataactccc taaattccct gagctaactc
4441 taagtcgacc atgccgttta tggttagcgc ctcccaaaaa ggaatggccg acttagagtt
4501 acctcttgac cgactttttc tttcctcccc cttacatttc gttaccacaa cacattccta
4561 tatccaaact ccaggtacat aactagtcga aatctctttt aaatctagtc aagaactaga
4621 tacttaacct tcatactgct tagagatagc ctttgaagct cttatttcgc tctttgtctc
4681 actctcacct tcctcctcct cctccaatcc tttttgctcg ggactagtcc aagactagtc
4741 aacgattagt atgccgagca ttcgcgataa cgatctccga aagtccccag agtactgtca
4801 ctatctcgag gcagttaagg acggggagct tacgctgccg gatttcaaga tagtaagccg
4861 acccgactag ctttaagcct agttactgac tagtccacag gacgacaatg gcgtgcctga
4921 tatccatcca tatgaagtct actgccgagt gaagggatgc ctcaagcgta cagtgagtct
4981 actgctatac tagtttctga ctagttatta actagttgac aggttccctc tgccaacaga
5041 aacatattgg tcaagcactt gaaggacaag aactcccacg gcatggagtt tacattgcac
5101 aatggacctc ccactatgaa ggaactgatg gaggccaaag gcaagtccta tctagattac
5161 ttggtgacta gtccctgact agtcaagtag catggtatga aggcttgttt gaaggcactg
5221 ttctcccaac cccgactcct accaagaagc gcaagcgagc tgcgtaagtt tcygtgagtc
5281 taactagtgt attagctaat atatagcagc accaagtcca aggaccacaa tactaagggt
5341 gtcgagaatt cgtgagtttc ttctcccatt tcaactagtc cttgactagt cactaactac
5401 tttgcagcaa cgagggtgaa gctggaaatg atcaggacaa tggcgagggc ccgtaagtac
5461 agccattcaa tgcagactag ttgctaacta atctgtgact agttcaagtg gtccgtacgc
5521 cgtgcatacc cctgtgactg gtaggaattt gagcaagcct gtcttgccgc gcgatgagaa
5581 aggaarggca agttacattc agccccgtac ctaggatcag tgctaattta taacctctag
5641 ccactcttta tgcagatccg ccgtgagggt agcaaggcag ctaaatcagc tggtgagaaa
5701 ggaaccatac cctgcaagac ctgtcgcaac gcaaagggca aaggtaagct atccaagcta
5761 gtttgggact agattctaac tagtctcagc accgtgtggt tcaaagccat attgcgagtt
5821 ttggcgcttt ttctcatcga ttgacgaggc aaagggagcg agtatgcaac ctcgtaagtc
5881 agactcagac aaagaccaac tagttattga ccagtcttct agaaggctct gttgtggatc
5941 ttgaggccct ggagagttcc tccaacaatc cggagacaag caagtcctcg tcggactagt
6001 cactaactag actctaacta gttgcagaca tggataatgc aaaagagaca agcaatgaag
6061 aaagtggtaa gacatttctc ctttgtggtt ctggactagt cttygactag tcacagtctt
6121 aaacaaggaa aatgagcatg aaaatgagga ggaaaaggct gctgagcccg aggaagtgca
6181 gggtgatggc agacatggta ggttaatacc ttgttagtta ttgctagtca ctgactagtc
6241 aataactagt ctctgaacac cttgcaatca ctccgtttgc gcagctgaac agtggtgagg
6301 ataatagtag taagttattc tagcttcaga gttataggag actagatact aactagtatt
6361 agttgcaact aacctggatc tcagagactt tggcctcaat ctagaatcta tctagttgtc
6421 aactagactg tggtatcatt gtcttttatt ttcctagtcc tggaactagc ttctaactag
6481 tctccctaat atgtggctgt cttgtttttt ttttttgttt ccctacccgg atatctagtc
6541 cccttctagg ttctgttaac ctctcgggct ctgatttagt ttaacgcaaa cctgagatta
6601 gtttctaact agtctctagg ttttctatcc acctttaatt gtaataataa atacaagcaa
6661 cgtttatacg tcaaaagcat ttataaactt ttaccctaaa gtagcttgct tgtgtgttta
6721 gtttataatt agtctcttat taatttgatg taggtaagcc cgccacaaat atatattttt
6781 aacaagatac cgtggaaaaa cttcgtgcta tcacaaaaca gtatacaaaa aataagctta
6841 acaatctatt ctccgcttgg tgatgctaaa gggctttcaa tagaccttgt aagtgaagga
6901 gatggagccg tcaatccgct accctgcctc tggtcagttg gtctcagcaa tgtaccctgt
6961 gagtcttgat agactagttg gtgactagtc tctgtagatg gaacaaatgg tgcttgagag
7021 ggcacgtatg gcagagctcc agtcatctgc ggaacatata ctgggcccgg gaagatctca
7081 tggtcatagc tgtttccgct gagggtttaa ttaagacctc agccgagaca gcagaatcac
7141 cgcccaagtt aagcctttgt gctgatcatg ctctcgaacg ggccaagttc gggaaaagca
7201 aaggagcgtt tagtgagggg caatttgact cacctcccag gcaacagatg aggggggcaa
7261 aaagaaagaa attttcgtga gtcaatatgg attccgagca tcattttctt gcggtctatc
7321 ttgctacgta tgttgatctt gacgctgtgg atcaagcaac gccactcgct cgctccatcg
7381 caggctggtc gcagacaaat taaaaggcgg caaactcgta cagccgcggg gttgtccgct
7441 gcaaagtaca gagtgataaa agccgccatg cgaccatcaa cgcgttgatg cccagctttt
7501 tcgatccgag aatccaccgt agaggcgata gcaagtaaag aaaagctaaa caaaaaaaaa
7561 tttctgcccc taagccatga aaacgagatg gggtggagca gaaccaagga aagagtcgcg
7621 ctgggctgcc gttccggaag gtgttgtaaa ggctcgacgc ccaaggtggg agtctaggag
7681 aagaatttgc atcgggagtg gggcgggtta cccctccata tccaatgaca gatatctacc
7741 agccaagggt ttgagcccgc ccgcttagtc gtcgtcctcg cttgcccctc cataaaagga
7801 tttcccctcc ccctcccaca aaattttctt tcccttcctc tccttgtccg cttcagtacg
7861 tatatcttcc cttccctcgc ttctctcctc catccttctt tcatccatct cctgctaact
7921 tctctgctca gcacctctac gcattactag ccgtagtatc tgagcacttc tcccttttat
7981 attccacaaa acataacaca accttcacca tggacaagaa gtatagcatc gggctggaca
8041 ttggaacgaa ctcggttggt tgggctgtga ttacggacga atacaaggtg ccatccaaga
8101 agtttaaggt cctgggaaac accgaccgtc actcaatcaa gaagaatctc attggagccc
8161 tgctcttcga tagtggggag accgccgaag ctactcgact gaagcgaacg gctcgccggc
8221 gttatacacg acgcaagaat cgcatctgct acctccagga gattttcagc aacgaaatgg
8281 ctaaggttga tgactcattc tttcatcgac tcgaagaaag tttcttggtc gaggaggata
8341 agaagcacga gcgccatccg atctttggta acattgtgga tgaggttgcc tatcacgaaa
8401 agtacccaac tatctatcat cttcgtaaga agctggtcga tagcacggac aaggctgatt
8461 tgcgacttat ctacctggca ctcgcgcaca tgattaagtt ccgcggccat tttcttatcg
8521 agggtgacct gaaccccgat aattctgacg ttgataagct cttcatccag ttggtccaaa
8581 cctacaatca gctgtttgag gaaaacccta ttaatgcatc tggcgtggac gccaaggcta
8641 tcctttcggc gcgcctgtct aagtcgcggc gtttggagaa ccttatcgca caactccccg
8701 gcgaaaagaa gaacggcctc ttcggtaatt tgattgcgtt gtcacttggt ctgactccta
8761 acttcaagag taattttgac ctggcagagg atgcgaagct ccagttgtct aaggatacgt
8821 atgatgacga tctcgacaac ttgcttgccc aaatcggtga ccagtacgct gatcttttcc
8881 tggccgctaa gaatctctca gatgcaatcc tgctcagtga cattttgcgg gtcaacaccg
8941 agattactaa ggcccccctg tcagctagta tgatcaagcg gtatgatgag caccatcagg
9001 acctcacctt gcttaaggcc ctcgtgcgtc agcaattgcc tgagaagtat aaggaaatct
9061 tctttgacca atccaagaac ggatacgcag ggtatattga tggcggtgcg agccaggagg
9121 aattctacaa gtttatcaag ccgattttgg agaagatgga cggcactgag gaactgctcg
9181 tcaagctgaa tcgcgaagat ttgcttcgta agcaacgaac gttcgacaac ggctccatcc
9241 cgcaccagat tcatctgggc gagctccacg ccatccttcg acgccaggaa gatttctacc
9301 catttctgaa ggacaaccgt gagaagatcg aaaagattct tacattccga atcccctact
9361 atgtgggacc tttggcccgt gggaattccc gatttgcttg gatgacccga aagagcgagg
9421 aaaccatcac tccgtggaac ttcgaggaag tcgtggacaa gggtgcatcc gcgcagagct
9481 tcattgagcg gatgaccaat tttgataaga accttccgaa tgaaaaggtc ctgccaaagc
9541 attcgctgct ctacgagtat ttcaccgtgt ataacgaact gactaaggtc aagtacgtga
9601 cggagggaat gcggaagcca gccttcctct caggggaaca aaagaaggct atcgtcgatt
9661 tgctttttaa gaccaatcgt aaagtgactg ttaagcagct gaaggaggat tatttcaaga
9721 agattgaatg tttcgactcc gtcgagatca gcggcgtgga agatcgcttt aacgcttccc
9781 tcggtaccta ccacgacctg ctcaagatca ttaaggacaa ggatttcctc gataacgagg
9841 aaaatgagga catcttggaa gatattgtcc tcacgttgac actttttgag gaccgcgaaa
9901 tgatcgagga acggctcaag acatatgccc atttgttcga cgataaggtg atgaagcagc
9961 tgaagcggcg tcgatacacc ggatggggtc gccttagccg gaagctgatc aacggcattc
10021 gagataagca atctggtaag actatcttgg atttccttaa gtcggacggc ttcgccaacc
10081 gcaattttat gcagcttatt cacgacgatt ccctgacgtt caaggaggac atccagaagg
10141 cacaagtctc aggacaaggg gattccctgc acgagcatat cgccaacctg gctggatccc
10201 cggcgatcaa gaaggggatt cttcagaccg tcaaggttgt cgacgagctg gtcaaggtga
10261 tgggccgtca taagccagaa aacatcgtga ttgagatggc ccgagaaaat cagaccactc
10321 aaaagggtca gaagaacagc cgcgagcgga tgaagcggat cgaggaaggc attaaggaac
10381 ttggttctca gatcctgaag gagcaccctg ttgaaaacac acagctccaa aatgagaagc
10441 tgtatctcta ctatttgcaa aatggacgcg acatgtacgt cgatcaggag ctcgacatta
10501 accggttgtc ggactacgat gttgaccata tcgtcccgca atccttcctt aaggacgata
10561 gcattgataa caaggtgctg actcgctcag ataagaaccg gggcaagtcc gacaatgttc
10621 caagcgagga agtggttaag aagatgaaga actactggcg ccaattgctt aatgccaagc
10681 tcatcacaca gcgcaagttt gacaacttga ccaaggccga gcggggaggg ctgagtgaac
10741 tcgataaggc tggcttcatc aagcgtcaac tcgtggagac gcgacagatc acaaagcacg
10801 ttgctcagat tctggactcc cggatgaaca caaagtacga cgagaatgat aagctcatcc
10861 gtgaagttaa ggtcattacc ctcaagtcta agttggtgtc ggatttccgc aaggacttcc
10921 aattttataa ggttcgggag atcaacaatt atcaccatgc acatgatgcg tacctcaacg
10981 cagtcgtggg aactgcgctc atcaagaagt atcccaagtt ggagtccgaa ttcgtctacg
11041 gggattataa ggtttacgac gtccgcaaga tgatcgccaa gagtgagcag gaaattggca
11101 aggccacggc taagtatttc ttttactcca acatcatgaa tttctttaag acggagatca
11161 cactcgccaa tggagaaatc cgtaagcgac ctttgattga gaccaacggc gagactggtg
11221 aaatcgtttg ggataagggg cgcgacttcg ctaccgtgcg gaaggttctg agcatgccgc
11281 aagtcaatat cgtcaagaaa accgaggtgc agacaggcgg tttctctaag gaatcgattc
11341 ttccaaagcg taactctgac aagctgatcg ctcgaaagaa ggattgggac cccaagaagt
11401 atggagggtt cgattctcct acagtggcat actcggttct cgttgtcgcg aaggttgaga
11461 agggaaagtc taagaagctg aagtcggtca aggaactgct cgggatcacc attatggagc
11521 gctccagctt cgaaaagaat cccatcgact ttctcgaggc caagggctat aaggaagtca
11581 agaaggatct tatcattaag ctgcctaagt actctttgtt cgagcttgaa aacggtcgaa
11641 agcgaatgct cgcatcggca ggagagttgc agaaggggaa tgaattggca cttccctcaa
11701 agtacgtgaa cttcctgtat ctcgcgtccc actacgagaa gctgaagggt agccctgagg
11761 acaacgaaca gaagcaactt tttgttgagc aacacaagca ttatctggat gagatcattg
11821 aacagatttc agagttcagt aagcgcgtca tcctcgccga tgctaatctc gacaaggtgt
11881 tgtcggccta caacaagcac cgtgacaagc cgatccgaga gcaggctgaa aatatcattc
11941 atctgttcac cctcactaac ttgggagcac cagcagcgtt caagtatttt gatacgacaa
12001 tcgaccgtaa gcgatacacg tccacaaagg aggtgcttga tgcgaccctg attcatcaat
12061 ccatcactgg gctctatgaa acccgtatcg accttagtca actggggggc gaccctccca
12121 agaagaagcg caaggtctga gcggacattc gatttatgcc gttatgactt ccttaaaaaa
12181 gcctttacga atgaaagaaa tggaattaga cttgttatgt agttgattct acaatggatt
12241 atgattcctg aacttcaaat ccgctgttca ttattaatct cagctcttcc cgtaaagcca
12301 atgttgaaac tattcgtaaa tgtacctcgt tttgcgtgta ccttgcttat cacgtgatat
12361 tacatgacct ggacagagtt ctgcgcgaaa gtcataacgt aaatcccggg cggtaggtgc
12421 gtcccgggcg gaaggtagtt ttctcgtcca ccccaacgcg tttatcaacc tcaactttca
12481 acaaccatca tgccaccaaa agcgcgtaaa acaaagcgag atttgattga gcaagagggc
12541 aggatccaat gcgcgattca agacattaaa aatggaaaat ttcaaaaaat tgcgcccgca
12601 gcgcgtgcat acaaaattca tcccaataca agtgtaattc ctcgtgtact gtgtaagcgc
12661 ccactaggta atatgacatg attacgaatt cgagctcggt acccggccgg ggatcctcta
12721 gacgtggagt taccagtgat tgaccaatgt tttatcttct acagttctgc ctgtctaccc
12781 cattctagct gtacctgact acagagtagt ttaattgtgg ttgaccccac agtcggaggc
12841 ggaggaatac agcaccgatg tggcctgtct ccatccagat tggcacgcaa tttttacacg
12901 cggaaaagat cgagatagag tacgacttta aatttagtcc ccggcggctt ctattttaga
12961 atatttgaga tttgattctc aagcaattga tttggttggg tcaccctcaa ttggataata
13021 tacctcattg ctcggctact tcaactcatc aatcaccgtc ataccccgca tataaccctc
13081 cattcccacg atgtcgtcca agtcgcaatt gacttacggt gctcgagcca gcaagcaccc
13141 caatcctctg gcaaagagac tttttgagat tgccgaagca aagaagacaa acgttaccgt
13201 ctctgctgat gtgacgacaa cccgagaact cctggacctc gctgaccgta cggaagctgt
13261 tggatccaat acatatgccg tctagcaatg gactaatcaa cttttgatga tacaggtctc
13321 ggtccctaca tcgccgtcat caagacacac atcgacatcc tcaccgattt cagcgtcgac
13381 actatcaatg gcctgaatgt gctggctcaa aagtacaact ttttgatctt cgaggaccgc
13441 aaattcatcg acatcggcaa taccgtccag aagcaatacc acggcggtgc tctgaggatc
13501 tccgaatggg cccacattat caactgcagc gttctccctg gcgagggcat cgtcgaggct
13561 ctggcccaga ccgcatctgc gcaagacttc ccctatggtc ctgagagagg actgttggtc
13621 ctggcagaga tgacccccaa aggatcgctg gctacgggcg agtataccaa ggcatcggtt
13681 gactacgctc gcaaatacaa gaacttcgtt atgggtttcg tgtcgacgcg ggccctgacg
13741 gaagtgcagt cggatgtgtc ttcagcctcg gaggatgaag atttcgtggt cttcacgacg
13801 ggtgtgaacc tctcttccaa aggagataag cttggacagc aataccagac tcctgcatcg
13861 gctattggac gcggtgccga ctttatcatc gccggtcgag gcatctacgc tgctcccgac
13921 ccggttgaag ctgcacagcg gtaccagaaa gaaggctggg aagcttatat ggccagagta
13981 tgcggcaagt catgatttcc tcttggagca aaagtgtagt gccagtacga gtgttgtgga
14041 ggaaggctgc atacattgtg cctgtcatta aacgatgagc tcgtccgtat tggcccctgt
14101 aatgccatgt tttccgcccc caatcgtcaa ggttttccct ttgttagatt cctaccagtc
14161 atctagcaag gcggccgcag ctagcacaat tgaggcatcc ccactaccgc attaagacct
14221 cagcgcggcc gcaaatttaa ataaaatgaa gtgaagttcc tatactttct agagaatagg
14281 aacttctata gtgagtcgaa taagggcgac acaaaattta ttctaaatgc ataataaata
14341 ctgataacat cttatagttt gtattatatt ttgtattatc gttgacatgt ataattttga
14401 tatcaaaaac tgattttccc tttattattt tcgagattta ttttcttaat tctctttaac
14461 aaactagaaa tattgtatat acaaaaaatc ataaataata gatgaatagt ttaattatag
14521 gtgttcatca atcgaaaaag caacgtatct tatttaaagt gcgttgcttt tttctcattt
14581 ataaggttaa ataattctca tatatcaagc aaagtgacag gcgcccttaa atattctgac
14641 aaatgctctt tccctaaact ccccccataa aaaaacccgc cgaagcgggt ttttacgtta
14701 tttgcggatt aacgattact cgttatcaga accgcccagg gggcccgagc ttaagactgg
14761 ccgtcgtttt acaacacaga aagagtttgt agaaacgcaa aaaggccatc cgtcaggggc
14821 cttctgctta gtttgatgcc tggcagttcc ctactctcgc cttccgcttc ctcgctcact
14881 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
14941 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
15001 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
15061 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
15121 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
15181 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
15241 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
15301 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
15361 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
15421 aggtatgtag gcggtgctac agagttcttg aagtggtggg ctaactacgg ctacactaga
15481 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
15541 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
15601 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
15661 gacgctcagt ggaacgacgc gcgcgtaact cacgttaagg gattttggtc atgagcttgc
15721 gccgtcccgt caagtcagcg taatgctctg ctt
//
载体图谱质粒图谱pdf版和相关资料下载
载体质粒应用举例
- 上一篇：pHIV-iRFP720-E2A-Luc
- 下一篇：pAAV-MCS2
