pG3H-U3Ub

LOCUS       pG3H-U3Ub               14350 bp ds-DNA     circular SYN 13-MAY-2021
DEFINITION  pGreen3 CRISPR/Cas9 .
ACCESSION   .
VERSION     .
KEYWORDS    pG3H-U3Ub
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14350)
  AUTHORS   Zhang Q, Zhang Y, Lu MH, Chai YP, Jiang YY, Zhou Y, Wang XC, Chen QJ
  TITLE     A Novel Ternary Vector System United with Morphogenic Genes Enhances
            CRISPR/Cas Delivery in Maize.
  JOURNAL   Plant Physiol. 2019 Sep 26. pii: pp.19.00767. doi: 
            10.1104/pp.19.00767.
  PUBMED    31558579
REFERENCE   2  (bases 1 to 14350)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 13, 2021 from SnapGene Server 1.1.58
            http://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..14350
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        1..380
                     /label=OsU3 promoter
                     /note="Oryza sativa (rice) snRNA U3 promoter"
     promoter        403..507
                     /gene="bla"
                     /label=AmpR promoter
     CDS             508..1368
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(726..745)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     misc_RNA        1517..1592
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        1658..3649
                     /label=Ubi promoter
                     /note="maize polyubiquitin gene promoter"
     CDS             3668..3733
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             3740..3760
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3785..7885
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     RBS             7798..7806
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             7886..7933
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     primer_bind     complement(8590..8606)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8590..8606)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8603..8625)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8614..8630
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(8638..8668)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8683..8704
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        8895..9571
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     9508..9528
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             9638..10663
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     polyA_signal    10704..10878
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    10956..10980
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     primer_bind     complement(11358..11377)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             11369..12178
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVSLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(11436..11455)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      12257..12343
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      12435..12462
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     rep_origin      12541..13129
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     13030..13049
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    14041..14065
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     14253..14275
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     14267..14284
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     14268..14284
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 aaggaatctt taaacatacg aacagatcac ttaaagttct tctgaagcaa cttaaagtta
       61 tcaggcttgc atggatcttg gaggaatcag atgtgcagtc agggaccata gcacaagaca
      121 ggcgtcttct actggtgcta ccagcaaatg ctggaagccg ggaacactgg gtacgttgga
      181 aaccacgtga tgtgaagaag taagataaac tgtaggagaa aagcatttcg tagtgggcca
      241 tgaagccttt caggacatgt attgcagtat gggccggccc attacgcaat tggacgacaa
      301 caaagtctag tattagtacc acctcggcta tccacataga tcaaagctga tttaaaagag
      361 ttgtgcagat gatccgtggc gtgagacctt cggggaaatg tgcgcggaac ccctatttgt
      421 ttatttttct aaatacattc aaatatgtat ccgctcatgg gacaataacc ctgataaatg
      481 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
      541 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
      601 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
      661 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
      721 gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc
      781 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
      841 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
      901 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
      961 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
     1021 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta
     1081 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
     1141 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
     1201 aaatctggag ccggtgagcg tggctctcgc ggtatcattg cagcactggg gccagatggt
     1261 aagccctccc gtatcgtagt tatctacacc acggggagtc aggcaactat ggatgaacga
     1321 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
     1381 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     1441 gtgaagatcc tttttgataa tcccatgacc aaaatccctt aacgtgagtt ttcgttccac
     1501 tgagcgtcag gtctcagttt tagagctaga aatagcaagt taaaataagg ctagtccgtt
     1561 atcaacttga aaaagtggca ccgagtcggt gctttttttt ttgtccttct gtttttttag
     1621 tcagtctctt ttttcagaag tacaacatct tgcatgcctg cagtgcagcg tgacccggtc
     1681 gtgcccctct ctagagataa tgagcattgc atgtctaagt tataaaaaat taccacatat
     1741 tttttttgtc acacttgttt gaagtgcagt ttatctatct ttatacatat atttaaactt
     1801 tactctacga ataatataat ctatagtact acaataatat cagtgtttta gagaatcata
     1861 taaatgaaca gttagacatg gtctaaagga caattgagta ttttgacaac aggactctac
     1921 agttttatct ttttagtgtg catgtgttct cctttttttt tgcaaatagc ttcacctata
     1981 taatacttca tccattttat tagtacatcc atttagggtt tagggttaat ggtttttata
     2041 gactaatttt tttagtacat ctattttatt ctattttagc ctctaaatta agaaaactaa
     2101 aactctattt tagttttttt atttaataat ttagatataa aatagaataa aataaagtga
     2161 ctaaaaatta aacaaatacc ctttaagaaa ttaaaaaaac taaggaaaca tttttcttgt
     2221 ttcgagtaga taatgccagc ctgttaaacg ccgtcgacga gtctaacgga caccaaccag
     2281 cgaaccagca gcgtcgcgtc gggccaagcg aagcagacgg cacggcatct ctgtcgctgc
     2341 ctctggaccc ctctcgagag ttccgctcca ccgttggact tgctccgctg tcggcatcca
     2401 gaaattgcgt ggcggagcgg cagacgtgag ccggcacggc aggcggcctc ctcctcctct
     2461 cacggcaccg gcagctacgg gggattcctt tcccaccgct ccttcgcttt cccttcctcg
     2521 cccgccgtaa taaatagaca ccccctccac accctctttc cccaacctcg tgttgttcgg
     2581 agcgcacaca cacacaacca gatctccccc aaatccaccc gtcggcacct ccgcttcaag
     2641 gtacgccgct cgtcctcccc cccccccctc tctaccttct ctagatcggc gttccggtcc
     2701 atggttaggg cccggtagtt ctacttctgt tcatgtttgt gttagatccg tgtttgtgtt
     2761 agatccgtgc tgctagcgtt cgtacacgga tgcgacctgt acgtcagaca cgttctgatt
     2821 gctaacttgc cagtgtttct ctttggggaa tcctgggatg gctctagccg ttccgcagac
     2881 gggatcgatt tcatgatttt ttttgtttcg ttgcataggg tttggtttgc ccttttcctt
     2941 tatttcaata tatgccgtgc acttgtttgt cgggtcatct tttcatgctt ttttttgtct
     3001 tggttgtgat gatgtggtct ggttgggcgg tcgttctaga tcggagtaga attctgtttc
     3061 aaactacctg gtggatttat taattttgga tctgtatgtg tgtgccatac atattcatag
     3121 ttacgaattg aagatgatgg atggaaatat cgatctagga taggtataca tgttgatgcg
     3181 ggttttactg atgcatatac agagatgctt tttgttcgct tggttgtgat gatgtggtgt
     3241 ggttgggcgg tcgttcattc gttctagatc ggagtagaat actgtttcaa actacctggt
     3301 gtatttatta attttggaac tgtatgtgtg tgtcatacat cttcatagtt acgagtttaa
     3361 gatggatgga aatatcgatc taggataggt atacatgttg atgtgggttt tactgatgca
     3421 tatacatgat ggcatatgca gcatctattc atatgctcta accttgagta cctatctatt
     3481 ataataaaca agtatgtttt ataattattt tgatcttgat atacttggat gatggcatat
     3541 gcagcagcta tatgtggatt tttttagccc tgccttcata cgctatttat ttgcttggta
     3601 ctgtttcttt tgtcgatgct caccctgttg tttggtgtta cttctgcagc cctaggccta
     3661 ctagatggat tacaaggacc acgacgggga ttacaaggac cacgacattg attacaagga
     3721 tgatgatgac aagatggctc cgaagaagaa gaggaaggtt ggcatccacg gggtgccagc
     3781 tgctgacaag aagtactcga tcggcctcga tattgggact aactctgttg gctgggccgt
     3841 gatcaccgac gagtacaagg tgccctcaaa gaagttcaag gtcctgggca acaccgatcg
     3901 gcattccatc aagaagaatc tcattggcgc tctcctgttc gacagcggcg agacggctga
     3961 ggctacgcgg ctcaagcgca ccgcccgcag gcggtacacg cgcaggaaga atcgcatctg
     4021 ctacctgcag gagattttct ccaacgagat ggcgaaggtt gacgattctt tcttccacag
     4081 gctggaggag tcattcctcg tggaggagga taagaagcac gagcggcatc caatcttcgg
     4141 caacattgtc gacgaggttg cctaccacga gaagtaccct acgatctacc atctgcggaa
     4201 gaagctcgtg gactccacag ataaggcgga cctccgcctg atctacctcg ctctggccca
     4261 catgattaag ttcaggggcc atttcctgat cgagggggat ctcaacccgg acaatagcga
     4321 tgttgacaag ctgttcatcc agctcgtgca gacgtacaac cagctcttcg aggagaaccc
     4381 cattaatgcg tcaggcgtcg acgcgaaggc tatcctgtcc gctaggctct cgaagtctcg
     4441 gcgcctcgag aacctgatcg cccagctgcc gggcgagaag aagaacggcc tgttcgggaa
     4501 tctcattgcg ctcagcctgg ggctcacgcc caacttcaag tcgaatttcg atctcgctga
     4561 ggacgccaag ctgcagctct ccaaggacac atacgacgat gacctggata acctcctggc
     4621 ccagatcggc gatcagtacg cggacctgtt cctcgctgcc aagaatctgt cggacgccat
     4681 cctcctgtct gatattctca gggtgaacac cgagattacg aaggctccgc tctcagcctc
     4741 catgatcaag cgctacgacg agcaccatca ggatctgacc ctcctgaagg cgctggtcag
     4801 gcagcagctc cccgagaagt acaaggagat cttcttcgat cagtcgaaga acggctacgc
     4861 tgggtacatt gacggcgggg cctctcagga ggagttctac aagttcatca agccgattct
     4921 ggagaagatg gacggcacgg aggagctgct ggtgaagctc aatcgcgagg acctcctgag
     4981 gaagcagcgg acattcgata acggcagcat cccacaccag attcatctcg gggagctgca
     5041 cgctatcctg aggaggcagg aggacttcta ccctttcctc aaggataacc gcgagaagat
     5101 cgagaagatt ctgactttca ggatcccgta ctacgtcggc ccactcgcta ggggcaactc
     5161 ccgcttcgct tggatgaccc gcaagtcaga ggagacgatc acgccgtgga acttcgagga
     5221 ggtggtcgac aagggcgcta gcgctcagtc gttcatcgag aggatgacga atttcgacaa
     5281 gaacctgcca aatgagaagg tgctccctaa gcactcgctc ctgtacgagt acttcacagt
     5341 ctacaacgag ctgactaagg tgaagtatgt gaccgagggc atgaggaagc cggctttcct
     5401 gtctggggag cagaagaagg ccatcgtgga cctcctgttc aagaccaacc ggaaggtcac
     5461 ggttaagcag ctcaaggagg actacttcaa gaagattgag tgcttcgatt cggtcgagat
     5521 ctctggcgtt gaggaccgct tcaacgcctc cctggggacc taccacgatc tcctgaagat
     5581 cattaaggat aaggacttcc tggacaacga ggagaatgag gatatcctcg aggacattgt
     5641 gctgacactc actctgttcg aggaccggga gatgatcgag gagcgcctga agacttacgc
     5701 ccatctcttc gatgacaagg tcatgaagca gctcaagagg aggaggtaca ccggctgggg
     5761 gaggctgagc aggaagctca tcaacggcat tcgggacaag cagtccggga agacgatcct
     5821 cgacttcctg aagagcgatg gcttcgcgaa ccgcaatttc atgcagctga ttcacgatga
     5881 cagcctcaca ttcaaggagg atatccagaa ggctcaggtg agcggccagg gggactcgct
     5941 gcacgagcat atcgcgaacc tcgctggctc gccagctatc aagaagggga ttctgcagac
     6001 cgtgaaggtt gtggacgagc tggtgaaggt catgggcagg cacaagcctg agaacatcgt
     6061 cattgagatg gcccgggaga atcagaccac gcagaagggc cagaagaact cacgcgagag
     6121 gatgaagagg atcgaggagg gcattaagga gctggggtcc cagatcctca aggagcaccc
     6181 ggtggagaac acgcagctgc agaatgagaa gctctacctg tactacctcc agaatggccg
     6241 cgatatgtat gtggaccagg agctggatat taacaggctc agcgattacg acgtcgatca
     6301 tatcgttcca cagtcattcc tgaaggatga ctccattgac aacaaggtcc tcaccaggtc
     6361 ggacaagaac cggggcaagt ctgataatgt tccttcagag gaggtcgtta agaagatgaa
     6421 gaactactgg cgccagctcc tgaatgccaa gctgatcacg cagcggaagt tcgataacct
     6481 cacaaaggct gagaggggcg ggctctctga gctggacaag gcgggcttca tcaagaggca
     6541 gctggtcgag acacggcaga tcactaagca cgttgcgcag attctcgact cacggatgaa
     6601 cactaagtac gatgagaatg acaagctgat ccgcgaggtg aaggtcatca ccctgaagtc
     6661 aaagctcgtc tccgacttca ggaaggattt ccagttctac aaggttcggg agatcaacaa
     6721 ttaccaccat gcccatgacg cgtacctgaa cgcggtggtc ggcacagctc tgatcaagaa
     6781 gtacccaaag ctcgagagcg agttcgtgta cggggactac aaggtttacg atgtgaggaa
     6841 gatgatcgcc aagtcggagc aggagattgg caaggctacc gccaagtact tcttctactc
     6901 taacattatg aatttcttca agacagagat cactctggcc aatggcgaga tccggaagcg
     6961 ccccctcatc gagacgaacg gcgagacggg ggagatcgtg tgggacaagg gcagggattt
     7021 cgcgaccgtc aggaaggttc tctccatgcc acaagtgaat atcgtcaaga agacagaggt
     7081 ccagactggc gggttctcta aggagtcaat tctgcctaag cggaacagcg acaagctcat
     7141 cgcccgcaag aaggactggg atccgaagaa gtacggcggg ttcgacagcc ccactgtggc
     7201 ctactcggtc ctggttgtgg cgaaggttga gaagggcaag tccaagaagc tcaagagcgt
     7261 gaaggagctg ctggggatca cgattatgga gcgctccagc ttcgagaaga acccgatcga
     7321 tttcctggag gcgaagggct acaaggaggt gaagaaggac ctgatcatta agctccccaa
     7381 gtactcactc ttcgagctgg agaacggcag gaagcggatg ctggcttccg ctggcgagct
     7441 gcagaagggg aacgagctgg ctctgccgtc caagtatgtg aacttcctct acctggcctc
     7501 ccactacgag aagctcaagg gcagccccga ggacaacgag cagaagcagc tgttcgtcga
     7561 gcagcacaag cattacctcg acgagatcat tgagcagatt tccgagttct ccaagcgcgt
     7621 gatcctggcc gacgcgaatc tggataaggt cctctccgcg tacaacaagc accgcgacaa
     7681 gccaatcagg gagcaggctg agaatatcat tcatctcttc accctgacga acctcggcgc
     7741 ccctgctgct ttcaagtact tcgacacaac tatcgatcgc aagaggtaca caagcactaa
     7801 ggaggtcctg gacgcgaccc tcatccacca gtcgattacc ggcctctacg agacgcgcat
     7861 cgacctgtct cagctcgggg gcgacaagcg gccagcggcg acgaagaagg cggggcaggc
     7921 gaagaagaag aagtgagctc agagctttcg ttcgtatcat cggtttcgac aacgttcgtc
     7981 aagttcaatg catcagtttc attgcgcaca caccagaatc ctactgagtt tgagtattat
     8041 ggcattggga aaactgtttt tcttgtacca tttgttgtgc ttgtaattta ctgtgttttt
     8101 tattcggttt tcgctatcga actgtgaaat ggaaatggat ggagaagagt taatgaatga
     8161 tatggtcctt ttgttcattc tcaaattaat attatttgtt ttttctctta tttgttgtgt
     8221 gttgaatttg aaattataag agatatgcaa acattttgtt ttgagtaaaa atgtgtcaaa
     8281 tcgtggcctc taatgaccga agttaatatg aggagtaaaa cacttgtagt tgtaccatta
     8341 tgcttattca ctaggcaaca aatatatttt cagacctaga aaagctgcaa atgttactga
     8401 atacaagtat gtcctcttgt gttttagaca tttatgaact ttcctttatg taattttcca
     8461 gaatccttgt cagattctaa tcattgcttt ataattatag ttatactcat ggatttgtag
     8521 ttgagtatga aaatattttt taatgcattt tatgacttgc caattgattg acaacgaatt
     8581 cgtaatcatg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
     8641 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
     8701 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
     8761 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attggctaga gcagcttgcc
     8821 aacatggtgg agcacgacac tctcgtctac tccaagaata tcaaagatac agtctcagaa
     8881 gaccaaaggg ctattgagac ttttcaacaa agggtaatat cgggaaacct cctcggattc
     8941 cattgcccag ctatctgtca cttcatcaaa aggacagtag aaaaggaagg tggcacctac
     9001 aaatgccatc attgcgataa aggaaaggct atcgttcaag atgcctctgc cgacagtggt
     9061 cccaaagatg gacccccacc cacgaggagc atcgtggaaa aagaagacgt tccaaccacg
     9121 tcttcaaagc aagtggattg atgtgaacat ggtggagcac gacactctcg tctactccaa
     9181 gaatatcaaa gatacagtct cagaagacca aagggctatt gagacttttc aacaaagggt
     9241 aatatcggga aacctcctcg gattccattg cccagctatc tgtcacttca tcaaaaggac
     9301 agtagaaaag gaaggtggca cctacaaatg ccatcattgc gataaaggaa aggctatcgt
     9361 tcaagatgcc tctgccgaca gtggtcccaa agatggaccc ccacccacga ggagcatcgt
     9421 ggaaaaagaa gacgttccaa ccacgtcttc aaagcaagtg gattgatgtg atatctccac
     9481 tgacgtaagg gatgacgcac aatcccacta tccttcgcaa gacccttcct ctatataagg
     9541 aagttcattt catttggaga ggacacgctg aaatcaccag tctctctcta caaatctatc
     9601 tctctcgagc tttcgcagat ccggggggca atgagatatg aaaaagcctg aactcaccgc
     9661 gacgtctgtc gagaagtttc tgatcgaaaa gttcgacagc gtctccgacc tgatgcagct
     9721 ctcggagggc gaagaatctc gtgctttcag cttcgatgta ggagggcgtg gatatgtcct
     9781 gcgggtaaat agctgcgccg atggtttcta caaagatcgt tatgtttatc ggcactttgc
     9841 atcggccgcg ctcccgattc cggaagtgct tgacattggg gagtttagcg agagcctgac
     9901 ctattgcatc tcccgccgtt cacagggtgt cacgttgcaa gacctgcctg aaaccgaact
     9961 gcccgctgtt ctacaaccgg tcgcggaggc tatggatgcg atcgctgcgg ccgatcttag
    10021 ccagacgagc gggttcggcc cattcggacc gcaaggaatc ggtcaataca ctacatggcg
    10081 tgatttcata tgcgcgattg ctgatcccca tgtgtatcac tggcaaactg tgatggacga
    10141 caccgtcagt gcgtccgtcg cgcaggctct cgatgagctg atgctttggg ccgaggactg
    10201 ccccgaagtc cggcacctcg tgcacgcgga tttcggctcc aacaatgtcc tgacggacaa
    10261 tggccgcata acagcggtca ttgactggag cgaggcgatg ttcggggatt cccaatacga
    10321 ggtcgccaac atcttcttct ggaggccgtg gttggcttgt atggagcagc agacgcgcta
    10381 cttcgagcgg aggcatccgg agcttgcagg atcgccacga ctccgggcgt atatgctccg
    10441 cattggtctt gaccaactct atcagagctt ggttgacggc aatttcgatg atgcagcttg
    10501 ggcgcagggt cgatgcgacg caatcgtccg atccggagcc gggactgtcg ggcgtacaca
    10561 aatcgcccgc agaagcgcgg ccgtctggac cgatggctgt gtagaagtac tcgccgatag
    10621 tggaaaccga cgccccagca ctcgtccgag ggcaaagaaa tagagtagat gccgaccggg
    10681 atctgtcgat cgacaagctc gagtttctcc ataataatgt gtgagtagtt cccagataag
    10741 ggaattaggg ttcctatagg gtttcgctca tgtgttgagc atataagaaa cccttagtat
    10801 gtatttgtat ttgtaaaata cttctatcaa taaaatttct aattcctaaa accaaaatcc
    10861 agtactaaaa tccagatccc ccgaattaat tcggcgttaa ttcagtacat taaaaacgtc
    10921 cgcaatgtgt tattaagttg tctaagcgtc aatttgttta caccacaata tatcctgcca
    10981 ccagccagcc aacagctccc cgaccggcag ctcggcacaa aatcaccact cgatacaggc
    11041 agcccatcag tccgggacgg cgtcagcggg agagccgttg taaggcggca gactttgctc
    11101 atgttaccga tgctattcgg aagaacggca actaagctgc cgggtttgaa acacggatga
    11161 tctcgcggag ggtagcatgt tgattgtaac gatgacagag cgttgctgcc tgtgatcacc
    11221 gcggctggca aacagctatt atgggtatta tgggtctagt ctctggcccg tgtctcaaaa
    11281 tctctgatgt tacattgcac aagataaaaa tatatcatca tgaacaataa aactgtctgc
    11341 ttacataaac agtaatacaa ggggtgttat gagccatatt caacgggaaa cgtcgaggcc
    11401 gcgattaaat tccaacatgg atgctgattt atatgggtat aaatgggctc gcgataatgt
    11461 cgggcaatca ggtgcgacaa tctatcgctt gtatgggaag cccgatgcgc cagagttgtt
    11521 tctgaaacat ggcaaaggta gcgttgccaa tgatgttaca gatgagatgg tcagactaaa
    11581 ctggctgacg gaatttatgc ctcttccgac catcaagcat tttatccgta ctcctgatga
    11641 tgcatggtta ctcaccactg cgatccccgg aaaaacagca ttccaggtat tagaagaata
    11701 tcctgattca ggtgaaaata ttgttgatgc gctggcagtg tccctgcgcc ggttgcattc
    11761 gattcctgtt tgtaattgtc cttttaacag cgatcgcgta tttcgtctcg ctcaggcgca
    11821 atcacgaatg aataacggtt tggttgatgc gagtgatttt gatgacgagc gtaatggctg
    11881 gcctgttgaa caagtctgga aagaaatgca taaacttttg ccattctcac cggattcagt
    11941 cgtcactcat ggtgatttct cacttgataa ccttattttt gacgagggga aattaatagg
    12001 ttgtattgat gttggacgag tcggaatcgc agaccgatac caggatcttg ccatcctatg
    12061 gaactgcctc ggtgagtttt ctccttcatt acagaaacgg ctttttcaaa aatatggtat
    12121 tgataatcct gatatgaata aattgcagtt tcatttgatg ctcgatgagt ttttctaatc
    12181 agaattggtt aattggttgt aacattattc agattggcgt ctaactacta agcgagagta
    12241 gggaactgcc aggcatcaaa taaaacgaaa ggctcagtcg gaagactggg cctttcgttt
    12301 tatctgttgt ttgtcggtga acgctctcct gagtaggaca aatccgccgg gagcggattt
    12361 gaacgttgtg aagcaacggc ccggagggtg gcgggcagga cgcccgccat aaactgccag
    12421 gcatcaaact aagcagaagg ccatcctgac ggatggcctt tttgcgtttc tacaaactct
    12481 tcctgttagg gccccgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
    12541 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
    12601 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
    12661 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
    12721 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
    12781 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
    12841 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
    12901 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
    12961 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
    13021 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
    13081 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
    13141 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
    13201 gttatcccct gattctgtgg ataaccgtat taccgctaga tagctggttg ccctcgccgc
    13261 tgggctggcg gccgtctatg gccctgcaaa cgcgccagaa acgccgtcga agccgtgtgc
    13321 gagacacccc ggccgccggc gttgtggata cctcgcggaa aacttggccc tcactgacag
    13381 atgaggggcg gacgttgaca cttgaggggc cgactcaccc ggcgcggcgt tgacagatga
    13441 ggggcaggct cgatttcggc cggcgacgtg gagctggcca gcctcgcaaa tcggcgaaaa
    13501 cgcctgattt tacgcgagtt tcccacagat gatgtggaca agcctgggga taagtgccct
    13561 gcggtattga cacttgaggg gcgcgactac tgacagatga ggggcgcgat ccttgacact
    13621 tgaggggcag agtgctgaca gatgaggggc gcacctattg acatttgagg ggctgtccac
    13681 aggcagaaaa tccagcattt gcaagggttt ccgcccgttt ttcggccacc gctaacctgt
    13741 cttttaacct gcttttaaac caatatttat aaaccttgtt tttaaccagg gctgcgccct
    13801 gtgcgcgtga ccgcgcacgc cgaagggggg tgccccccct tctcgaaccc tcccggtcga
    13861 gtcatggtct agcatggatc tcggggacgt cacccataat acccataata gctgtttgcc
    13921 agtactttga tcccgagggg aaccctgtgg ttggcatgca catacaaatg gacgaacgga
    13981 taaacctttt cacgcccttt taaatatccg attattctaa taaacgctct tttctcttag
    14041 gtttacccgc caatatatcc tgtcaaacac tgatagttta aactgaaggc gggaaacgac
    14101 aatctgatcc aagctcaagc tgctctagca ttcgccattc aggctgcgca actgttggga
    14161 agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc
    14221 aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc
    14281 cagtgccaag cttagtaatt catccaggtc accaagttct aggattttca gaactgcaac
    14341 ttattttatc
//