| 质粒名称: | pGBW-m4092694 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 153907 |
| 存储实验室: | Ginkgo Bioworks, Benjie Chen |
|
载体骨架基本信息 |
|
| 载体名称: | pG9m-2 backbone |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 1968 bp |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 细菌表达 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 低拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | fragment SARS-CoV-2 orf1a mature peptide 3 to orf1a stop |
| 物种来源: | Severe acute respiratory syndrome coronavirus 2 |
| 插入大小: | 4928 bp |
| 基因突变: | wt |
| GenBank编号 | -- |
| 启动子 | Promoter | t7 consensus from NEB, G is +1 |
| 标签/融合蛋白: | -- |
| 克隆方法: | -- |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pGBW-m4092694 was a gift from Ginkgo Bioworks & Benjie Chen (Addgene plasmid # 153907 ; http://n2t.net/addgene:153907 ; RRID:Addgene_153907) |
买家导航
pGBWm4092694载体基本信息
pGBWm4092694载体图谱质粒图谱和多克隆位点信息
pGBWm4092694载体简介
pGBWm4092694载体序列
LOCUS pGBW-m4092694 8300 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Bacterial expression plasmid for fragment SARS-CoV-2 orf1a mature
peptide 3 to orf1a stop.
ACCESSION .
VERSION .
KEYWORDS pGBW-m4092694
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8300)
TITLE SARS-CoV-2 protein E. coli expression constructs, tagged, for
protein expression and/or purification
REFERENCE 2 (bases 1 to 8300)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..8300
/organism="synthetic DNA construct"
/mol_type="other DNA"
terminator 5183..5230
/label=T7 terminator
/note="transcription terminator for bacteriophage T7 RNA
polymerase"
terminator 5273..5304
/label=tonB terminator
/note="bidirectional E. coli tonB-P14 transcription
terminator"
promoter 5305..5407
/label=cat promoter
/note="promoter of the E. coli cat gene encoding
chloramphenicol acetyltransferase"
CDS 5408..6268
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESLRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLATIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGSLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(5626..5645)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
CDS 6269..6460
/codon_start=1
/gene="rop"
/product="Rop protein, which maintains plasmids at low copy
number"
/label=rop
/translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
DELYRSCLARFGDDGENL"
terminator 6484..6511
/label=T7Te terminator
/note="phage T7 early transcription terminator"
rep_origin complement(6523..7110)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
terminator 7132..7161
/label=T3Te terminator
/note="phage T3 early transcription terminator"
promoter 7200..7217
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
1 catattcagt ggatggttat gttcacacct ttagtacctt tctggataac aattgcttat
61 atcatttgta tttccacaaa gcatttctat tggttcttta gtaattacct aaagagacgt
121 gtagtcttta atggtgtttc ctttagtact tttgaagaag ctgcgctgtg cacctttttg
181 ttaaataaag aaatgtatct aaagttgcgt agtgatgtgc tattacctct tacgcaatat
241 aatagatact tagctcttta taataagtac aagtatttta gtggagcaat ggatacaact
301 agctacagag aagctgcttg ttgtcatctc gcaaaggctc tcaatgactt cagtaactca
361 ggttctgatg ttctttacca accaccacaa acctctatca cctcagctgt tttgcagagt
421 ggttttagaa aaatggcatt cccatctggt aaagttgagg gttgtatggt acaagtaact
481 tgtggtacaa ctacacttaa cggtctttgg cttgatgacg tagtttactg tccaagacat
541 gtgatctgca cctctgaaga catgcttaac cctaattatg aagatttact cattcgtaag
601 tctaatcata atttcttggt acaggctggt aatgttcaac tcagggttat tggacattct
661 atgcaaaatt gtgtacttaa gcttaaggtt gatacagcca atcctaagac acctaagtat
721 aagtttgttc gcattcaacc aggacagact ttttcagtgt tagcttgtta caatggttca
781 ccatctggtg tttaccaatg tgctatgagg cccaatttca ctattaaggg ttcattcctt
841 aatggttcat gtggtagtgt tggttttaac atagattatg actgtgtctc tttttgttac
901 atgcaccata tggaattacc aactggagtt catgctggca cagacttaga aggtaacttt
961 tatggacctt ttgttgacag gcaaacagca caagcagctg gtacggacac aactattaca
1021 gttaatgttt tagcttggtt gtacgctgct gttataaatg gagacaggtg gtttctcaat
1081 cgatttacca caactcttaa tgactttaac cttgtggcta tgaagtacaa ttatgaacct
1141 ctaacacaag accatgttga catactagga cctctttctg ctcaaactgg aattgccgtt
1201 ttagatatgt gtgcttcatt aaaagaatta ctgcaaaatg gtatgaatgg acgtaccata
1261 ttgggtagtg ctttattaga agatgaattt acaccttttg atgttgttag acaatgctca
1321 ggtgttactt tccaaagtgc agtgaaaaga acaatcaagg gtacacacca ctggttgtta
1381 ctcacaattt tgacttcact tttagtttta gtccagagta ctcaatggtc tttgttcttt
1441 tttttgtatg aaaatgcctt tttacctttt gctatgggta ttattgctat gtctgctttt
1501 gcaatgatgt ttgtcaaaca taagcatgca tttctctgtt tgtttttgtt accttctctt
1561 gccactgtag cttattttaa tatggtctat atgcctgcta gttgggtgat gcgtattatg
1621 acatggttgg atatggttga tactagtttg tctggtttta agctaaaaga ctgtgttatg
1681 tatgcatcag ctgtagtgtt actaatcctt atgacagcaa gaactgtgta tgatgatggt
1741 gctaggagag tgtggacact tatgaatgtc ttgacactcg tttataaagt ttattatggt
1801 aatgctttag atcaagccat ttccatgtgg gctcttataa tctctgttac ttctaactac
1861 tcaggtgtag ttacaactgt catgtttttg gccagaggta ttgtttttat gtgtgttgag
1921 tattgcccta ttttcttcat aactggtaat acacttcagt gtataatgct agtttattgt
1981 ttcttaggct atttttgtac ttgttacttt ggcctctttt gtttactcaa ccgctacttt
2041 agactgactc ttggtgttta tgattactta gtttctacac aggagtttag atatatgaat
2101 tcacagggac tactcccacc caagaatagc atagatgcct tcaaactcaa cattaaattg
2161 ttgggtgttg gtggcaaacc ttgtatcaaa gtagccactg tacagtctaa aatgtcagat
2221 gtaaagtgca catcagtagt cttactctca gttttgcaac aactcagagt agaatcatca
2281 tctaaattgt gggctcaatg tgtccagtta cacaatgaca ttctcttagc taaagatact
2341 actgaagcct ttgaaaaaat ggtttcacta ctttctgttt tgctttccat gcagggtgct
2401 gtagacataa acaagctttg tgaagaaatg ctggacaaca gggcaacctt acaagctata
2461 gcctcagagt ttagttccct tccatcatat gcagcttttg ctactgctca agaagcttat
2521 gagcaggctg ttgctaatgg tgattctgaa gttgttctta aaaagttgaa gaagtctttg
2581 aatgtggcta aatctgaatt tgaccgtgat gcagccatgc aacgtaagtt ggaaaagatg
2641 gctgatcaag ctatgaccca aatgtataaa caggctagat ctgaggacaa gagggcaaaa
2701 gttactagtg ctatgcagac aatgcttttc actatgctta gaaagttgga taatgatgca
2761 ctcaacaaca ttatcaacaa tgcaagagat ggttgtgttc ccttgaacat aatacctctt
2821 acaacagcag ccaaactaat ggttgtcata ccagactata acacatataa aaatacgtgt
2881 gatggtacaa catttactta tgcatcagca ttgtgggaaa tccaacaggt tgtagatgca
2941 gatagtaaaa ttgttcaact tagtgaaatt agtatggaca attcacctaa tttagcatgg
3001 cctcttattg taacagcttt aagggccaat tctgctgtca aattacagaa taatgagctt
3061 agtcctgttg cactacgaca gatgtcttgt gctgccggta ctacacaaac tgcttgcact
3121 gatgacaatg cgttagctta ctacaacaca acaaagggag gtaggtttgt acttgcactg
3181 ttatccgatt tacaggattt gaaatgggct agattcccta agagtgatgg aactggtact
3241 atctatacag aactggaacc accttgtagg tttgttacag acacacctaa aggtcctaaa
3301 gtgaagtatt tatactttat taaaggatta aacaacctaa atagaggtat ggtacttggt
3361 agtttagctg ccacagtacg tctacaagct ggtaatgcaa cagaagtgcc tgccaattca
3421 actgtattat ctttctgtgc ttttgctgta gatgctgcta aagcttacaa agattatcta
3481 gctagtgggg gacaaccaat cactaattgt gttaagatgt tgtgtacaca cactggtact
3541 ggtcaggcaa taacagttac accggaagcc aatatggatc aagaatcctt tggtggtgca
3601 tcgtgttgtc tgtactgccg ttgccacata gatcatccaa atcctaaagg attttgtgac
3661 ttaatagact ggccccctga atctccagac aaccaatatc acttaaataa gtgatagtct
3721 taatactagt ttttagacta gtcattggag aacagatgat tgatgtctta gggccggaga
3781 aacgcagacg gcgtaccaca caggaaaaga tcgcaattgt tcagcagagc tttgaaccgg
3841 ggatgacggt ctccctcgtt gcccggcaac atggtgtagc agccagccag ttatttctct
3901 ggcgtaagca ataccaggaa ggaagtctta ctgctgtcgc cgccggagaa caggttgttc
3961 ctgcctctga acttgctgcc gccatgaagc agattaaaga actccagcgc ctgctcggca
4021 agaaaacgat ggaaaatgaa ctcctcaaag aagccgttga atatggacgg gcaaaaaagt
4081 ggatagcgca cgcgccctta ttgcccgggg atggggagta agcttagtca gccgttgtct
4141 ccgggtgtcg cgtgcgcagt tgcacgtcat tctcagacga accgatgact ggatggatgg
4201 ccgccgcagt cgtcacactg atgatacgga tgtgcttctc cgtatacacc atgttatcgg
4261 agagctgcca acgtatggtt atcgtcgggt atgggcgctg cttcgcagac aggcagaact
4321 tgatggtatg cctgcgatca atgccaaacg tgtttaccgg atcatgcgcc agaatgcgct
4381 gttgcttgag cgaaaacctg ctgtaccgcc atcgaaacgg gcacatacag gcagagtggc
4441 cgtgaaagaa agcaatcagc gatggtgctc tgacgggttc gagttctgct gtgataacgg
4501 agagagactg cgtgtcacgt tcgcgctgga ctgctgtgat cgtgaggcac tgcactgggc
4561 ggtcactacc ggcggcttca acagtgaaac agtacaggac gtcatgctgg gagcggtgga
4621 acgccgcttc ggcaacgatc ttccgtcgtc tccagtggag tggctgacgg ataatggttc
4681 atgctaccgg gctaatgaaa cacgccagtt cgcccggatg ttgggacttg aaccgaagaa
4741 cacggcggtg cggagtccgg agagtaacgg aatagcagag agcttcgtga aaacgataaa
4801 gcgtgactac atcagtatca tgcccaaacc agacgggtta acggcagcaa agaaccttgc
4861 agaggcgttc gagcattata acgaatggca tccgcatagt gcgctgggtt atcgctcgcc
4921 acgggaatat ctgcggcagc gggcttgtaa tgggttaagt gataacagat gtctggaaat
4981 ataggggcaa atccacttaa aaggtaagta tgtacaaata cctacaactt gtgctaatga
5041 ccctgtgggt tttacactta aaaacacagt ctgtaccgtc tgcggtatgt ggaaaggtta
5101 tggctgtagt tgtgatcaac tccgcgaacc catgcttcag tcagctgatg cacaatcgtt
5161 tttaaacggg tttgcggtgt aactagcata accccttggg gcctctaaac gggtcttgag
5221 gggttttttg catcactgac catttaaatc atacctgacc tccatagcag aaagtcaaaa
5281 gcctccgacc ggaggctttt gacttgatcg gcacgtaaga ggttccaact ttcaccataa
5341 tgaaataaga tcactaccgg gcgtattttt tgagttatcg agattttcag gagctaagga
5401 agctaaaatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
5461 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
5521 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
5581 acgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
5641 attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
5701 tgacttggtt gagtactcac cagtcacaga aaagcatctc acggatggca tgacagtaag
5761 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctggc
5821 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
5881 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
5941 cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
6001 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggatcact
6061 tctgcgctcg gccctcccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
6121 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gcatcgtagt
6181 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
6241 aggtgcctca ctgattaagc attggtaagt gaccaaacag gaaaaaaccg cccttaacat
6301 ggcccgcttt atcagaagcc agacattaac gcttctggag aaactcaacg agctggacgc
6361 ggatgaacag gcagacatct gtgaatcgct tcacgaccac gctgatgagc tttaccgcag
6421 ctgcctcgcg cgtttcggtg atgacggtga aaacctctga tgagggccca aatgtaatca
6481 cctggctcac cttcgggtgg gcctttctgc gttgctggcg tttttccata ggctccgccc
6541 ccctgacgag catcacaaaa atcgatgctc aagtcagagg tggcgaaacc cgacaggact
6601 ataaagatac caggcgttta cccctggaag ctccctcgtg cgctctcctg ttccgaccct
6661 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
6721 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
6781 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
6841 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
6901 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
6961 aagaacagta tttggtatct gcgctctgct gaagccagtt acctcggaaa aagagttggt
7021 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
7081 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgattttcta ccgaagaaag
7141 gcccacccgt gaaggtgagc cagtgagttg attgcagtcc agttacgctg gagtcaagct
7201 aatacgactc actatagaaa attgttaata attggttgaa gcagttaatt aaagttacac
7261 ttgtgttcct ttttgttgct gctattttct atttaataac acctgttcat gtcatgtcta
7321 aacatactga cttttcaagt gaaatcatag gatacaaggc tattgatggt ggtgtcactc
7381 gtgacatagc atctacagat acttgttttg ctaacaaaca tgctgatttt gacacatggt
7441 ttagccagcg tggtggtagt tatactaatg acaaagcttg cccattgatt gctgcagtca
7501 taacaagaga agtgggtttt gtcgtgcctg gtttgcctgg cacgatatta cgcacaacta
7561 atggtgactt tttgcatttc ttacctagag tttttagtgc agttggtaac atctgttaca
7621 caccatcaaa acttatagag tacactgact ttgcaacatc agcttgtgtt ttggctgctg
7681 aatgtacaat ttttaaagat gcttctggta agccagtacc atattgttat gataccaatg
7741 tactagaagg ttctgttgct tatgaaagtt tacgccctga cacacgttat gtgctcatgg
7801 atggctctat tattcaattt cctaacacct accttgaagg ttctgttaga gtggtaacaa
7861 cttttgattc tgagtactgt aggcacggca cttgtgaaag atcagaagct ggtgtttgtg
7921 tatctactag tggtagatgg gtacttaaca atgattatta cagatcttta ccaggagttt
7981 tctgtggtgt agatgctgta aatttactta ctaatatgtt tacaccacta attcaaccta
8041 ttggtgcttt ggacatatca gcatctatag tagctggtgg tattgtagct atcgtagtaa
8101 catgccttgc ctactatttt atgaggttta gaagagcttt tggtgaatac agtcatgtag
8161 ttgcctttaa tactttacta ttccttatgt cattcactgt actctgttta acaccagttt
8221 actcattctt acctggtgtt tattctgtta tttacttgta cttgacattt tatcttacta
8281 atgatgtttc ttttttagca
//
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