| 质粒名称: | pIB166 |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 90189 |
| 存储实验室: | Indranil Biswas |
|
载体骨架基本信息 |
|
| 载体名称: | pSH71 (pJRS1315) |
| 载体出品公司: | June Scott (PMID 12867436) |
| 空载体大小: | 4000 bp |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 细菌表达 |
| 筛选标记: | -- |
| 载体抗性: | 氯霉素25 μg/mL |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | -- |
| 物种来源: | -- |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子: | -- |
| 标签/融合蛋白: |
无（主干上的N端子） 无（主干上的C端子） |
| 克隆方法: | -- |
| 5’克隆位点: | -- |
| 3’克隆位点: | -- |
| 5’测序引物 | -- |
| 3’测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pIB166 was a gift from Indranil Biswas (Addgene plasmid # 90189 ; http://n2t.net/addgene:90189 ; RRID:Addgene_90189) |
| 参考文献部分: | Shuttle expression plasmids for genetic studies in Streptococcus mutans. Biswas I, Jha JK, Fromm N. Microbiology. 2008 Aug;154(Pt 8):2275-82. doi: 10.1099/mic.0.2008/019265-0. 10.1099/mic.0.2008/019265-0 PubMed 18667560 |
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载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pIB166 4006 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION E. coli - Streptococci shuttle plasmid for gene expression in
streptococci with P23 promoter.
ACCESSION .
VERSION .
KEYWORDS pIB166
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4006)
AUTHORS Biswas I, Jha JK, Fromm N
TITLE Shuttle expression plasmids for genetic studies in Streptococcus
mutans.
JOURNAL Microbiology. 2008 Aug;154(Pt 8):2275-82. doi:
10.1099/mic.0.2008/019265-0.
PUBMED 18667560
REFERENCE 2 (bases 1 to 4006)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..4006
/organism="synthetic DNA construct"
/mol_type="other DNA"
CDS 2952..2963
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
ORIGIN
1 agcttaagtg attagtcaaa gaatggtgat gacaattgta caggttatca ataaagccat
61 tgatactctc attcttatcc cgtatttatt aacttctcct agtattagta attatcatgg
121 ctgtcatggc gcattaacgg aataaagggt gtgcttaaat cgggccattt tgcgtaataa
181 gaaaaaggat taattatgag cgaattgaat taataataag gtaatagatt tacattagaa
241 aatgaaaggg gattttatgc gtgagaatgt tacagtctat cccggcaata gttaccctta
301 ttatcaagat aagaaagaaa aggatttttc gctacgctca aatcctttaa aaaaacacaa
361 aagaccacat tttttaatgt ggtcttttat tcttcaacta aagcacccat tagttcaaca
421 aacgaaaatt ggataaagtg ggatattttt aaaatatata tttatgttac agtaatattg
481 acttttaaaa aaggattgat tataatgaag aaagcagaca agtaagcctc ctaaattcac
541 tttagataaa aatttaggag gcatatcaaa tgaactttaa taaaattcac tttagataaa
601 aatttaggag gcatatcaaa tgaactttaa taaaattgat ttagacaatt ggaagagaaa
661 agagatattt aatcattatt tgaaccaaca aacgactttt agtataacca cagaaattga
721 tattagtgtt ttataccgaa acataaaaca agaaggatat aaattttacc ctgcatttat
781 tttcttagtg acaagggtga taaactcaaa tacagctttt agaactggtt acaatagcga
841 cggagagtta ggttattggg ataagttaga gccactttat acaatttttg atggtgtatc
901 taaaacattc tctggtattt ggactcctgt aaagaatgac ttcaaagagt tttatgattt
961 atacctttct gatgtagaga aatataatgg ttcggggaaa ttgtttccca aaacacctat
1021 acctgaaaat gctttttctc tttctattat tccatggact tcatttactg ggtttaactt
1081 aaatatcaat aataatagta attaccttct acccattatt acagcaggaa aattcattaa
1141 taaaggtaat tcaatatatt taccgctatc tttacaggta catcattctg tttgtgatgg
1201 ttatcatgca ggattgttta tgaactctat tcaggaattg tcagataggc ctaatgactg
1261 gcttttataa tatgagataa tgccgactgt actttttaca gtcggttttc taatgtcact
1321 aacctgcccc gttagttgaa gaaggttttt atattacagc tccagatcca tatccttctt
1381 tttctgaacc gacttctcct ttttcgcttc tttattccaa ttgctttatt gacgttgagc
1441 ctcggaaccc ttaacaatcc caaaacttgt cgactttcgt caggggggct tttatttatt
1501 caataatccc tcctctcaat aaatctattg ttgtacttaa ttcaacttcc atttctctgt
1561 atctttcaat acgctctttt aagtccttaa tttctttttt taattcctca ttttcagcaa
1621 ataactcttt ttctttgttt gtcattttat ttcccccgtt tcagcatcaa gaacctttgc
1681 ataacttgct ctatatccac actgataatt gccctcaaac cataatctaa aggcgctaga
1741 gtttgttgaa acaatatctt ttacatcatt cgtatttaaa attccaaact ccgctcccct
1801 aaggcgaata aaagccatta actcttttgt atttaccaaa ttatagtcat ccactatatc
1861 taaaagtaaa ttcttcaatt ttcttttttg gctttcatca agtgttatat agcggtcaat
1921 atcaaaatca ttaatgttca aaatatcttt tttgtcgtat atatgtttat tcttagcaat
1981 agcgtccttt gattcatgag tcaaatattc atatgaacct ttgatataat caagtatctc
2041 aacatgagca actgaactat tccccaattt tcgcttaatc ttgttcctaa cgctttctat
2101 tgttacagga tttcgtgcaa tatatataac gtgatagtgt ggttttttat agtgctttcc
2161 atttcgtata acatcactac tattccatgt atctttatct tttttttcgt ccatatcgtg
2221 taaaggactg acagccatag atacgcccaa actctctaat ttttctttcc aatcattagg
2281 aattgagtca ggatataata aaaatccaaa atttctagct ttagtatttt taatagccat
2341 gatataatta ccttatcaaa aacaagtagc gaaaactcgt atccttctaa aaacgcgagc
2401 tttcgcttat tttttttgtt ctgattcctt tcttgcatat tcttctatag ctaacgccgc
2461 aaccgcagat tttgaaaaac ctttttgttt cgccatatct gttaattttt tatcttgctc
2521 ttttgtcaga gaaatcataa ctcttttttt cgattctgaa atcaccattt aaaaaactcc
2581 aatcaaataa ttttataaaa ttagtgtatc actttgtaat cataaaaaca acaataaagc
2641 tacttaaata tagatttata aaaaacgttg gcgaaaacgt tggcgattcg ttggcgattg
2701 aaaaacccct caaacccttg agccagttgg gatagagcgt ttttggcaca aaaattggca
2761 ctcggcactt aatggggggt cgtagtacgg aagcaaaatt cgcttccttt ccccccattt
2821 ttttccaaat tccaaatttt tttcaaaaat tttccagcgc taccgctcgg caaaattgca
2881 agcaattttt aaaatcaaac ccatgaggga atttcattcc ctcaaactcc cttgagcctc
2941 ctccaaccga aatagaagga cgctgcgctt attatttcat tcagtcatcg gctttcataa
3001 tctaacagac aacatcttcg ctgcaaagcc acgctacgct caagggcttt tacgctacga
3061 taacgcctgt tttaacgatt atgccgataa ctaaacgaaa taaacgctaa aacgtctcag
3121 aaacgatttt gagacgtttt aataaaaaat cgaccgaaaa ataaatataa atcggatata
3181 caatcggcaa ttgacgaaac tgcaaaatat cctgtaaagg atacggattt tatgaccgat
3241 gatgaagaaa agaatttgaa acgtttgtct gatttggagg aaggtttaca ccgtaaaagg
3301 ttaatctcct atggtggttt gttaaaagaa atacataaaa aattaaacct tgatgacaca
3361 gaagaaggcg atttgattca tacagatgat gacgaaaaag ccgatgaaga tggattttct
3421 attattgcaa tgtggaattg ggaacggaaa aattatttta ttaaagagta gttcaacaaa
3481 cgggccagtt tgttgaagat tagatgctat aattgttatt aaaaggattg aaggatgctt
3541 aggaagacga gttattaata gctgaataag aacggtgctc tccaaatatt cttatttaga
3601 aaagcaaatc taaaattatc tgaaaaggga atgagaatag tgaatggacc aataataatg
3661 actagagaag aaagaatgaa gattgttcat gaaattaagg aacgaatatt ggataaatat
3721 ggggatgatg ttaaggctat tggtgtttat ggctctcttg gtcgtcagac tgatgggccc
3781 cgaaaagccc tgacaaccct tgttcctaaa aaggaataag cgttcggtca gtaaataata
3841 gaaataaaaa atcagaccta agactgatga caaaaagaga aaattttgat aaaatagtct
3901 tagaattaaa ttaaaaaggg aggccaaata taatgaaaaa tatgaatgac aatgatgttg
3961 gatccccgcg gtacccggga attctagagc tcgagatcta tcgata
//
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