| 质粒名称: | pIMAY |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 68939 |
| 存储实验室: | Tim Foster |
|
载体骨架基本信息 |
|
| 载体名称: | -- |
| 载体出品公司: | -- |
| 载体抗性: | 氯霉素 |
| 空载体大小: | 5743 bp |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 葡萄球菌等位基因交换载体 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 低拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | -- |
| 物种来源: | -- |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | -- |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | T7 |
| 3' 测序引物 | T3 |
|
文献引用方法 |
|
| 材料和方法部分: | pIMAY was a gift from Tim Foster (Addgene plasmid # 68939 ; http://n2t.net/addgene:68939 ; RRID:Addgene_68939) |
| 参考文献部分: | Transforming the untransformable: application of direct transformation to manipulate genetically Staphylococcus aureus and Staphylococcus epidermidis. Monk IR, Shah IM, Xu M, Tan MW, Foster TJ. MBio. 2012 Mar 20;3(2). pii: e00277-11. doi: 10.1128/mBio.00277-11. Print 2012. 10.1128/mBio.00277-11 PubMed 22434850 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pIMAY 5743 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION E. coli/staphylococcal temperature-sensitive plasmid for allelic
exchange.
ACCESSION .
VERSION .
KEYWORDS pIMAY
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5743)
AUTHORS Monk IR, Shah IM, Xu M, Tan MW, Foster TJ
TITLE Transforming the untransformable: application of direct
transformation to manipulate genetically Staphylococcus aureus and
Staphylococcus epidermidis.
JOURNAL MBio. 2012 Mar 20;3(2). pii: e00277-11. doi: 10.1128/mBio.00277-11.
Print 2012.
PUBMED 22434850
REFERENCE 2 (bases 1 to 5743)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..5743
/organism="synthetic DNA construct"
/mol_type="other DNA"
CDS complement(157..168)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
promoter 2675..2693
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
misc_feature 2706..2813
/label=MCS
/note="pBluescript multiple cloning site"
primer_bind 2722..2738
/label=pBluescriptKS
/note="For pBluescript vector"
primer_bind 2723..2739
/label=KS primer
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(2773..2789)
/label=SK primer
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(2773..2789)
/label=pBluescriptSK
/note="For pBluescript vector"
primer_bind complement(2821..2840)
/label=T7
/note="T7 promoter, forward primer"
promoter complement(2822..2840)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(3213..3230)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(3347..3892)
/direction=LEFT
/label=p15A ori
/note="Plasmids containing the medium-copy-number p15A
origin of replication can be propagated in E. coli cells
that contain a second plasmid with the ColE1 origin.
"
primer_bind complement(3427..3446)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
oriT 4034..4143
/note="incP origin of transfer"
protein_bind 4957..4975
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O1 for the tetR and tetA genes"
protein_bind 5042..5060
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O1 for the tetR and tetA genes"
CDS 5085..5708
/codon_start=1
/gene="tetR from transposon Tn10"
/product="tetracycline repressor TetR"
/label=TetR
/note="TetR binds to the tetracycline operator tetO to
inhibit transcription. This inhibition can be relieved by
adding tetracycline or doxycycline."
/translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
primer_bind complement(5158..5177)
/label=Tet-R
/note="Tetracycline resistance gene, reverse primer"
ORIGIN
1 gcatgcgttt tagcgtttat ttcgtttagt tatcggcata atcgttaaaa caggcgttat
61 cgtagcgtaa aagcccttga gcgtagcgtg gctttgcagc gaagatgttg tctgttagat
121 tatgaaagcc gatgactgaa tgaaataata agcgcagcgc ccttctattt cggttggagg
181 aggctcaagg gagtatgagg gaatgaaatt ccctcatggg tttgatttta aaaattgctt
241 gcaattttgc cgagcggtag cgctggaaaa tttttgaaaa aaatttggaa tttggaaaaa
301 aatgggggga aaggaagcga attttgcttc cgtactacga ccccccatta agtgccgagt
361 gccaattttt gtgccaaaaa cgctctatcc caactggctc aagggtttaa ggggtttttc
421 aatcgccaac gaatcgccaa cgttttcgcc aacgtttttt ataaatctat atttaagtag
481 ctttattgtt gtttttatga ttacaaagtg atacactaac tttataaaat tatttgattg
541 gagtttttta aatggtgatt tcagaatcga aaaaaagagt tatgatttct ctgacaaaag
601 agcaagataa aaaattaaca gatatggcga aacaaaaagg tttttcaaaa tctgcggttg
661 cggcgttagc tatagaagaa tatgcaagaa aggaatcaga acaaaaaaaa taagcgaaag
721 ctcgcgtttt tagaaggata cgagttttcg ctacttgttt ttgataaggt aattatatca
781 tggctattaa aaatactaaa gctagaaatt ttggattttt attatatcct gactcaattc
841 ctaatgattg gaaagaaaaa ttagagagtt tgggcgtatc tatggctgtc agtcctttac
901 acgatatgga cgaaaaaaaa gataaagata catggaataa tagtaatatt atacaaaatg
961 gaaagcacta taaaaaacca cactatcacg ttatatatat tgcacgaaat cctgtaacaa
1021 tagaaagcgt taggaacaag attaagcgaa aattggggaa tagttcagtt gctcatgttg
1081 agatacttga ttatatcaaa ggttcatatg aatatttgac tcatgaatca aaggacgcta
1141 ttgctaagaa taaacatata tacgacaaaa aagatatttt gaacattaat gattttgata
1201 ttgaccgcta tataacactt gatgaaagcc aaaaaagaga attgaagaat ttacttttag
1261 atatagtgga tgactataat ttggtaaata caaaagattt aatggctttt attcgcctta
1321 ggggagcgga gtttggaatt ttaaatacga atgatgtaaa agatattgtt tcaacaaact
1381 ctagcgcctt tagattatgg tttgagggca attatcagtg tggatataga gcaagttatg
1441 caaaggttct tgatgctgaa acgggggaaa taaaatgaca aacaaagaaa aagagttatt
1501 tgctgaaaat gaggaattaa aaaaagaaat taaggactta aaagagcgta ttgaaagata
1561 cagagaaatg gaagttgaat taagtacaac aatagattta ttgagaggag ggattattga
1621 ataaataaaa gccccctgac gaaagtcgaa gggggttttt attttggttt gatgttgcga
1681 ttaatagcaa tacattctat aatagaaggt atggaggatg ttatataatg agacagaatt
1741 atgatgatca tatgtcaact aacggggcag gttagtgaca ttagaaaacc gactgtaaaa
1801 agtacagtcg gcattatctc atattataaa agccagtcat taggcctatc tgacaattcc
1861 tgaatagagt tcataaacaa tcctgcatga taaccatcac aaacagaatg atgtacctgt
1921 aaagatagcg gtaaatatat tgaattacct ttattaatga attttcctgc tgtaataatg
1981 ggtagaaggt aattactatt attattgata tttaagttaa acccagtaaa tgaagtccat
2041 ggaataatag aaagagaaaa agcattttca ggtataggtg ttttgggaaa caatttcccc
2101 gaaccattat atttctctac atcagaaagg tataaatcat aaaactcttt gaagtcattc
2161 tttacaggag tccaaatacc agagaatgtt ttagatacac catcaaaaat tgtataaagt
2221 ggctctaact tatcccaata acctaactct ccgtcgctat tgtaaccagt tctaaaagct
2281 gtatttgagt ttatcaccct tgtcactaag aaaataaatg cagggtaaaa tttatatcct
2341 tcttgtttta tgtttcggta taaaacacta atatcaattt ctgtggttat actaaaagtc
2401 gtttgttggt tcaaataatg attaaatatc tcttttctct tccaattgtc taaatcaatt
2461 ttattaaagt tcatgggttt cactctcctt ctacattttt taacctaata atgccaaata
2521 ccgtttgcca cccctctctt tgataattat aatattggcg aaattcgctt ctaaagatga
2581 aacgcaatat tatatgcttg ctttatcggc cgtatgtgat tataccagcc ccctcactac
2641 atgtcaagaa taaactgcca aagcataatg ggataattaa ccctcactaa agggaacaaa
2701 agctgggtac cgggcccccc ctcgaggtcg acggtatcga taagcttgat atcgaattcc
2761 tgcagcccgg gggatccact agttctagag cggccgccac cgcggtggag ctccaattcg
2821 ccctatagtg agtcgtatta cgacgtccca gggcttcccg gtatcaacag ggacaccagg
2881 atttatttat tctgcgaagt gatcttccgt cacaggtatt tattcggcgc aaagtgcgtc
2941 gggtgatgct gccaacttac tgatttagtg tatgatggtg tttttgaggt gctccagtgg
3001 cttctgtttc tatcagctgt ccctcctgtt cagctactga cggggtggtg cgtaacggca
3061 aaagcaccgc cggacatcag cgctagcgga gtgtatactg gcttactatg ttggcactga
3121 tgagggtgtc agtgaagtgc ttcatgtggc aggagaaaaa aggctgcacc ggtgcgtcag
3181 cagaatatgt gatacaggat atattccgct tcctcgctca ctgactcgct acgctcggtc
3241 gttcgactgc ggcgagcgga aatggcttac gaacggggcg gagatttcct ggaagatgcc
3301 aggaagatac ttaacaggga agtgagaggg ccgcggcaaa gccgtttttc cataggctcc
3361 gcccccctga caagcatcac gaaatctgac gctcaaatca gtggtggcga aacccgacag
3421 gactataaag ataccaggcg tttccccctg gcggctccct cgtgcgctct cctgttcctg
3481 cctttcggtt taccggtgtc attccgctgt tatggccgcg tttgtctcat tccacgcctg
3541 acactcagtt ccgggtaggc agttcgctcc aagctggact gtatgcacga accccccgtt
3601 cagtccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggaaagacat
3661 gcaaaagcac cactggcagc agccactggt aattgattta gaggagttag tcttgaagtc
3721 atgcgccggt taaggctaaa ctgaaaggac aagttttggt gactgcgctc ctccaagcca
3781 gttacctcgg ttcaaagagt tggtagctca gagaaccttc gaaaaaccgc cctgcaaggc
3841 ggttttttcg ttttcagagc aagagattac gcgcagacca aaacgatctc aagaagatca
3901 tcttattaat cagataaaat atttctagat ttcagtgcaa tttatctctt caaatgtagc
3961 acctgaagtc agccccatac gatataagtt gtaattctcc gccgcttgcc ctcatctgtt
4021 acgccggcgg tagccggcca gcctcgcaga gcaggattcc cgttgagcac cgccaggtgc
4081 gaataaggga cagtgaagaa ggaacacccg ctcgcgggtg ggcctacttc acctatcctg
4141 cccggctgac gccgttggat acaccaagga aagtctacac gaaccctttg gcaaaatcct
4201 gtatatcgtg cgaaaaagga tggatatacc gaaaaaatcg ctataatgac cccgaagcag
4261 ggttatgcag cggaaaagcg ctgcttccct gctgttttgt ggaatatcta ccgactggaa
4321 acaggcaaat gcaggaaatt actgaactga ggggacaggc gagaggagat cttgatctaa
4381 tgattcaaac ccttgtgaac ttctttagaa caaaagaggt tcgtaacaag attttcttca
4441 cactagcaat gttagtaatt tttaaaatag ggacttatat accagctcca ggagtaaatc
4501 ctgcagcttt tgataatccc caaggttctc aaggtgccac tgagttatta aatacttttg
4561 gtggcggagc cttgaaacga ttttctattt ttgcaatggg tattgtaccc tacatcactg
4621 catcaatcgt aatgcaatta ttacaaatgg atattgtccc taaattctca gaatgggcaa
4681 aacaaggtga agtaggtaga agaaagttaa ataacgttac tcgttattta gcaatttctt
4741 tagcatttat ccaatctata ggtatggcat tccaatttaa taattatctc aaaggtgcgc
4801 tgattatcaa tcagtcaatt atgagttatt tattaatagc actagttttg acagcaggaa
4861 ctgctttctt aatatggctt ggtgatcaaa tcactcagtt cggtgttggt aatggtattt
4921 ctattatcat attcccatca agcttatttt aattatactc tatcaatgat agagtgtcaa
4981 tatttttttt agtttttcat gaactcgagg ggatccaaat aaaaaactag tttgacaaat
5041 aactctatca atgatagagt gtcaacaaaa aggaggaatt aatgatgtct agattagata
5101 aaagtaaagt gattaacagc gcattagagc tgcttaatga ggtcggaatc gaaggtttaa
5161 caacccgtaa actcgcccag aagctaggtg tagagcagcc tacattgtat tggcatgtaa
5221 aaaataagcg ggctttgctc gacgccttag ccattgagat gttagatagg caccatactc
5281 acttttgccc tttagaaggg gaaagctggc aagatttttt acgtaataac gctaaaagtt
5341 ttagatgtgc tttactaagt catcgcgatg gagcaaaagt acatttaggt acacggccta
5401 cagaaaaaca gtatgaaact ctcgaaaatc aattagcctt tttatgccaa caaggttttt
5461 cactagagaa tgcattatat gcactcagcg ctgtggggca ttttacttta ggttgcgtat
5521 tggaagatca agagcatcaa gtcgctaaag aagaaaggga aacacctact actgatagta
5581 tgccgccatt attacgacaa gctatcgaat tatttgatca ccaaggtgca gagccagcct
5641 tcttattcgg ccttgaattg atcatatgcg gattagaaaa acaacttaaa tgtgaaagtg
5701 ggtcttaaaa gcagcataac ctttttccgt gatggtaact tca
//
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