| 质粒名称: | pIVT-dCas9-EGFP |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 174442 |
| 存储实验室: | Michael Lampson |
|
载体骨架基本信息 |
|
| 载体名称: | pIVT |
| 载体类型: | 体外转录模板 |
| 载体抗性: | 氨苄霉素100 μg/mL |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 低拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | Cas9 Endonuclease Dead,dCas9 |
| 物种来源: | Streptococcus pyogenes |
| 插入大小: | 4104 bp |
| 启动子: | T7 SP6 |
| 标签/融合蛋白: | EGFP（插入件上的C端子） |
| 克隆方法: | Ligation Independent 克隆 |
| 5’测序引物 | CAGGAAACAGCTATGAC |
| 3’测序引物 | GTAAAACGACGGCCAGT |
|
文献引用方法 |
|
| 材料和方法部分: | pIVT-dCas9-EGFP was a gift from Michael Lampson (Addgene plasmid # 174442 ; http://n2t.net/addgene:174442 ; RRID:Addgene_174442) |
| 参考文献部分: | Parallel pathways for recruiting effector proteins determine centromere drive and suppression. Kumon T, Ma J, Akins RB, Stefanik D, Nordgren CE, Kim J, Levine MT, Lampson MA. Cell. 2021 Sep 16;184(19):4904-4918.e11. doi: 10.1016/j.cell.2021.07.037. Epub 2021 Aug 24. 10.1016/j.cell.2021.07.037 PubMed 34433012 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pIVT-dCas9-EGFP 7891 bp ds-DNA circular SYN 22-SEP-2021
DEFINITION in vitro transcription template for dCas9-EGFP.
ACCESSION .
VERSION .
KEYWORDS pIVT-dCas9-EGFP
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7891)
TITLE Parallel pathways for recruiting effector proteins determine
centromere drive and suppression
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 7891)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Sep 22, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..7891
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind complement(10..28)
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
promoter 96..200
/gene="bla"
/label=AmpR promoter
CDS 201..1061
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(419..438)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 1232..1820
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 1721..1740
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 1974..1991
/label=L4440
/note="L4440 vector, forward primer"
protein_bind 2108..2129
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 2144..2174
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 2182..2198
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 2187..2209
/label=M13/pUC Reverse
/note="In lacZ gene"
primer_bind 2206..2222
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 2206..2222
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
promoter 2246..2264
/label=SP6 promoter
/note="promoter for bacteriophage SP6 RNA polymerase"
primer_bind 2246..2263
/label=SP6
/note="SP6 promoter, forward primer"
primer_bind 2276..2295
/label=T7
/note="T7 promoter, forward primer"
promoter 2276..2294
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
5'UTR 2337..2379
/label=Xenopus globin 5'-UTR
/note="translational enhancer from the 5'-UTR of the major
beta-globin gene of Xenopus laevis"
regulatory 2437..2446
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 2443..6546
/codon_start=1
/product="catalytically dead mutant of the Cas9
endonuclease from the Streptococcus pyogenes Type II
CRISPR/Cas system"
/label=dCas9
/note="RNA-guided DNA-binding protein that lacks
endonuclease activity due to the D10A mutation in the RuvC
catalytic domain and the H840A mutation in the HNH
catalytic domain"
/translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
DATLIHQSITGLYETRIDLSQLGGD"
CDS 6562..7278
/codon_start=1
/product="enhanced GFP"
/label=EGFP
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
primer_bind complement(6607..6628)
/label=EGFP-N
/note="EGFP, reverse primer"
primer_bind complement(6868..6887)
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
primer_bind 7215..7236
/label=EGFP-C
/note="EGFP, forward primer"
3'UTR 7309..7434
/label=Xenopus globin 3'-UTR
/note="3'-UTR of the major beta-globin gene of Xenopus
laevis"
primer_bind complement(7497..7514)
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind complement(7497..7513)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(7506..7528)
/label=M13/pUC Forward
/note="In lacZ gene"
primer_bind complement(7722..7741)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 7841..7863
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
ORIGIN
1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
61 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
121 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
181 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
241 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
301 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
361 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
421 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
481 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
541 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
601 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
661 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
721 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
781 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
841 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
901 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
961 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
1021 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
1081 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
1141 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
1201 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
1261 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
1321 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
1381 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
1441 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
1501 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
1561 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
1621 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
1681 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
1741 atagtcctat cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
1801 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
1861 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
1921 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
1981 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
2041 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
2101 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
2161 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg
2221 accatgatta cgccaagctc caacgattta ggtgacacta tagaagagaa ggaattaata
2281 cgactcacta tagggagaga gagagaatta ccctcactaa agggaggaga agcttgcttg
2341 ttctttttgc agaagctcag aataaacgct caactttggc agatcaagct tgcatgcctg
2401 caggtcgact atagggagac ccaagcttgg cgcgccgcca ccatggacaa gaagtactcc
2461 attgggctcg ctatcggcac aaacagcgtc ggctgggccg tcattacgga cgagtacaag
2521 gtgccgagca aaaaattcaa agttctgggc aataccgatc gccacagcat aaagaagaac
2581 ctcattggcg ccctcctgtt cgactccggg gagacggccg aagccacgcg gctcaaaaga
2641 acagcacggc gcagatatac ccgcagaaag aatcggatct gctacctgca ggagatcttt
2701 agtaatgaga tggctaaggt ggatgactct ttcttccata ggctggagga gtcctttttg
2761 gtggaggagg ataaaaagca cgagcgccac ccaatctttg gcaatatcgt ggacgaggtg
2821 gcgtaccatg aaaagtaccc aaccatatat catctgagga agaagcttgt agacagtact
2881 gataaggctg acttgcggtt gatctatctc gcgctggcgc atatgatcaa atttcgggga
2941 cacttcctca tcgaggggga cctgaaccca gacaacagcg atgtcgacaa actctttatc
3001 caactggttc agacttacaa tcagcttttc gaagagaacc cgatcaacgc atccggagtt
3061 gacgccaaag caatcctgag cgctaggctg tccaaatccc ggcggctcga aaacctcatc
3121 gcacagctcc ctggggagaa gaagaacggc ctgtttggta atcttatcgc cctgtcactc
3181 gggctgaccc ccaactttaa atctaacttc gacctggccg aagatgccaa gcttcaactg
3241 agcaaagaca cctacgatga tgatctcgac aatctgctgg cccagatcgg cgaccagtac
3301 gcagaccttt ttttggcggc aaagaacctg tcagacgcca ttctgctgag tgatattctg
3361 cgagtgaaca cggagatcac caaagctccg ctgagcgcta gtatgatcaa gcgctatgat
3421 gagcaccacc aagacttgac tttgctgaag gcccttgtca gacagcaact gcctgagaag
3481 tacaaggaaa ttttcttcga tcagtctaaa aatggctacg ccggatacat tgacggcgga
3541 gcaagccagg aggaatttta caaatttatt aagcccatct tggaaaaaat ggacggcacc
3601 gaggagctgc tggtaaagct taacagagaa gatctgttgc gcaaacagcg cactttcgac
3661 aatggaagca tcccccacca gattcacctg ggcgaactgc acgctatcct caggcggcaa
3721 gaggatttct accccttttt gaaagataac agggaaaaga ttgagaaaat cctcacattt
3781 cggataccct actatgtagg ccccctcgcc cggggaaatt ccagattcgc gtggatgact
3841 cgcaaatcag aagagaccat cactccctgg aacttcgagg aagtcgtgga taagggggcc
3901 tctgcccagt ccttcatcga aaggatgact aactttgata aaaatctgcc taacgaaaag
3961 gtgcttccta aacactctct gctgtacgag tacttcacag tttataacga gctcaccaag
4021 gtcaaatacg tcacagaagg gatgagaaag ccagcattcc tgtctggaga gcagaagaaa
4081 gctatcgtgg acctcctctt caagacgaac cggaaagtta ccgtgaaaca gctcaaagaa
4141 gactatttca aaaagattga atgtttcgac tctgttgaaa tcagcggagt ggaggatcgc
4201 ttcaacgcat ccctgggaac gtatcacgat ctcctgaaaa tcattaaaga caaggacttc
4261 ctggacaatg aggagaacga ggacattctt gaggacattg tcctcaccct tacgttgttt
4321 gaagataggg agatgattga agaacgcttg aaaacttacg ctcatctctt cgacgacaaa
4381 gtcatgaaac agctcaagag gcgccgatat acaggatggg ggcggctgtc aagaaaactg
4441 atcaatggga tccgagacaa gcagagtgga aagacaatcc tggattttct taagtccgat
4501 ggatttgcca acaggaactt catgcagttg atccatgatg actctctcac ctttaaggag
4561 gacatccaga aagcacaagt ttctggccag ggggacagtc ttcacgagca catcgctaat
4621 cttgcaggta gcccagctat caaaaaggga atactgcaga ccgttaaggt cgtggatgaa
4681 ctcgtcaaag taatgggaag gcataagccc gagaatatcg ttatcgagat ggcccgagag
4741 aaccaaacta cccagaaggg acagaagaac agtagggaaa ggatgaagag gattgaagag
4801 ggtataaaag aactggggtc ccaaatcctt aaggaacacc cagttgaaaa cacccagctt
4861 cagaatgaga agctctacct gtactacctg cagaacggca gggacatgta cgtggatcag
4921 gaactggaca tcaatcggct ctccgactac gacgtggatg ctatcgtgcc ccagtctttt
4981 ctcaaagatg attctattga taataaagtg ttgacaagat ccgataaaaa tagagggaag
5041 agtgataacg tcccctcaga agaagttgtc aagaaaatga aaaattattg gcggcagctg
5101 ctgaacgcca aactgatcac acaacggaag ttcgataatc tgactaaggc tgaacgaggt
5161 ggcctgtctg agttggataa agccggcttc atcaaaaggc agcttgttga gacacgccag
5221 atcaccaagc acgtggccca aattctcgat tcacgcatga acaccaagta cgatgaaaat
5281 gacaaactga ttcgagaggt gaaagttatt actctgaagt ctaagctggt ctcagatttc
5341 agaaaggact ttcagtttta taaggtgaga gagatcaaca attaccacca tgcgcatgat
5401 gcctacctga atgcagtggt aggcactgca cttatcaaaa aatatcccaa gcttgaatct
5461 gaatttgttt acggagacta taaagtgtac gatgttagga aaatgatcgc aaagtctgag
5521 caggaaatag gcaaggccac cgctaagtac ttcttttaca gcaatattat gaattttttc
5581 aagaccgaga ttacactggc caatggagag attcggaagc gaccacttat cgaaacaaac
5641 ggagaaacag gagaaatcgt gtgggacaag ggtagggatt tcgcgacagt ccggaaggtc
5701 ctgtccatgc cgcaggtgaa catcgttaaa aagaccgaag tacagaccgg aggcttctcc
5761 aaggaaagta tcctcccgaa aaggaacagc gacaagctga tcgcacgcaa aaaagattgg
5821 gaccccaaga aatacggcgg attcgattct cctacagtcg cttacagtgt actggttgtg
5881 gccaaagtgg agaaagggaa gtctaaaaaa ctcaaaagcg tcaaggaact gctgggcatc
5941 acaatcatgg agcgatcaag cttcgaaaaa aaccccatcg actttctcga ggcgaaagga
6001 tataaagagg tcaaaaaaga cctcatcatt aagcttccca agtactctct ctttgagctt
6061 gaaaacggcc ggaaacgaat gctcgctagt gcgggcgagc tgcagaaagg taacgagctg
6121 gcactgccct ctaaatacgt taatttcttg tatctggcca gccactatga aaagctcaaa
6181 gggtctcccg aagataatga gcagaagcag ctgttcgtgg aacaacacaa acactacctt
6241 gatgagatca tcgagcaaat aagcgaattc tccaaaagag tgatcctcgc cgacgctaac
6301 ctcgataagg tgctttctgc ttacaataag cacagggata agcccatcag ggagcaggca
6361 gaaaacatta tccacttgtt tactctgacc aacttgggcg cgcctgcagc cttcaagtac
6421 ttcgacacca ccatagacag aaagcggtac acctctacaa aggaggtcct ggacgccaca
6481 ctgattcatc agtcaattac ggggctctat gaaacaagaa tcgacctctc tcagctcggt
6541 ggagacgcta gcggctctag aatggtgagc aagggcgagg agctgttcac cggggtggtg
6601 cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag
6661 ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag
6721 ctgcccgtgc cctggcccac cctcgtgacc accctgacct acggcgtgca gtgcttcagc
6781 cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac
6841 gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg
6901 aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag
6961 gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc
7021 atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag
7081 gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc
7141 gtgctgctgc ccgacaacca ctacctgagc acccagtccg ccctgagcaa agaccccaac
7201 gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc
7261 atggacgagc tgtacaagta gtgactgact aggatctggt taccactaaa ccagcctcaa
7321 gaacacccga atggagtctc taagctacat aataccaact tacactttac aaaatgttgt
7381 cccccaaaat gtagccattc gtatctgctc ctaataaaaa gaaagtttct tcacattcta
7441 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa ancccccccc ccccaagggc gaattcactg
7501 gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt
7561 gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct
7621 tcccaacagt tgcgcagcct gaatggcgaa tggcgcctga tgcggtattt tctccttacg
7681 catctgtgcg gtatttcaca ccgcatatgg tgcactctca gtacaatctg ctctgatgcc
7741 gcatagttaa gccagccccg acacccgcca acacccgctg acgcgccctg acgggcttgt
7801 ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag
7861 aggttttcac cgtcatcacc gaaacgcgcg a
//
