| 质粒名称: | pJMP1363 |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 119279 |
| 存储实验室: | Carol Gross, Jason Peters, Oren Rosenberg |
|
载体骨架基本信息 |
|
| 载体名称: | R6Kgamma |
| 载体出品公司: | -- |
| 空载体大小: | -- |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 细菌表达 |
| 筛选标记: | 氯霉素 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | BW25141 |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | dcas9 |
| 物种来源: | -- |
| 插入大小: | -- |
| 基因突变: | D10A and H840A |
| GenBank编号 | -- |
| 启动子: | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | Gibson克隆 |
| 5’克隆位点: | -- |
| 3’克隆位点: | -- |
| 5’测序引物 | -- |
| 3’测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pJMP1363 was a gift from Carol Gross & Jason Peters & Oren Rosenberg (Addgene plasmid # 119279 ; http://n2t.net/addgene:119279 ; RRID:Addgene_119279) |
| 参考文献部分: | Enabling genetic analysis of diverse bacteria with Mobile-CRISPRi. Peters JM, Koo BM, Patino R, Heussler GE, Hearne CC, Qu J, Inclan YF, Hawkins JS, Lu CHS, Silvis MR, Harden MM, Osadnik H, Peters JE, Engel JN, Dutton RJ, Grossman AD, Gross CA, Rosenberg OS. Nat Microbiol. 2019 Jan 7. pii: 10.1038/s41564-018-0327-z. doi: 10.1038/s41564-018-0327-z. 10.1038/s41564-018-0327-z PubMed 30617347 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pJMP1363 9796 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION for stabilizing ICE in the presence of rapI expression.
ACCESSION .
VERSION .
KEYWORDS pJMP1363
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9796)
AUTHORS Peters JM, Koo BM, Patino R, Heussler GE, Hearne CC, Qu J, Inclan
YF, Hawkins JS, Lu CHS, Silvis MR, Harden MM, Osadnik H, Peters JE,
Engel JN, Dutton RJ, Grossman AD, Gross CA, Rosenberg OS
TITLE Enabling genetic analysis of diverse bacteria with Mobile-CRISPRi.
JOURNAL Nat Microbiol. 2019 Jan 7. pii: 10.1038/s41564-018-0327-z. doi:
10.1038/s41564-018-0327-z.
PUBMED 30617347
REFERENCE 2 (bases 1 to 9796)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..9796
/organism="synthetic DNA construct"
/mol_type="other DNA"
protein_bind 1..48
/label=FRT
/bound_moiety="FLP recombinase from the Saccharomyces
cerevisiae 2u plasmid"
/note="FLP-mediated recombination occurs in the 8-bp core
sequence TCTAGAAA (Turan and Bode, 2011)."
CDS 1140..5243
/codon_start=1
/product="catalytically dead mutant of the Cas9
endonuclease from the Streptococcus pyogenes Type II
CRISPR/Cas system"
/label=dCas9
/note="RNA-guided DNA-binding protein that lacks
endonuclease activity due to the D10A mutation in the RuvC
catalytic domain and the H840A mutation in the HNH
catalytic domain"
/translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
DATLIHQSITGLYETRIDLSQLGGD"
CDS 5253..5342
/codon_start=1
/product="3 tandem Myc epitope tags"
/label=3xMyc
/translation="EQKLISEEDLEQKLISEEDLEQKLISEEDL"
oriT 6512..6620
/note="incP origin of transfer"
rep_origin 6889..7277
/label=R6K gamma ori
/note="gamma replication origin from E. coli plasmid R6K;
requires the R6K initiator protein pi for replication"
CDS complement(7578..8438)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 8201..8220
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(8439..8543)
/gene="bla"
/label=AmpR promoter
terminator 9463..9557
/label=lambda t0 terminator
/note="transcription terminator from phage lambda"
terminator 9660..9746
/gene="Escherichia coli rrnB"
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
ORIGIN
1 gaagtaccta ttccgaagtt cctattctct agaaagtata ggaacttcag agcgcttttg
61 aagctgatgc tcgaggcgta gaggatctgg agctgtaata taaaaacctt cttcaactaa
121 cggggcaggt tagtgacatt agaaaaccga ctgtaaaaag tacagtcggc attatctcat
181 attataaaag ccagtcatta ggcctatctg acaattcctg aatagagttc ataaacaatc
241 ctgcatgata accatcacaa acagaatgat gtacctgtaa agatagcggt aaatatattg
301 aattaccttt attaatgaat tttcctgctg taataatggg tagaaggtaa ttactattat
361 tattgatatt taagttaaac ccagtaaatg aagtccatgg aataatagaa agagaaaaag
421 cattttcagg tataggtgtt ttgggaaaca atttccccga accattatat ttctctacat
481 cagaaaggta taaatcataa aactctttga agtcattctt tacaggagtc caaataccag
541 agaatgtttt agatacacca tcaaaaattg tataaagtgg ctctaactta tcccaataac
601 ctaactctcc gtcgctattg taaccagttc taaaagctgt atttgagttt atcacccttg
661 tcactaagaa aataaatgca gggtaaaatt tatatccttc ttgttttatg tttcggtata
721 aaacactaat atcaatttct gtggttatac taaaagtcgt ttgttggttc aaataatgat
781 taaatatctc ttttctcttc caattgtcta aatcaatttt attaaagttc atttgatatg
841 cctcctaaat ttttatctaa agtgaattta ggaggcttac ttgtctgctt tcttcattag
901 aatcaatcct tttttaaaag tcaatattac tgtaacataa atatatattt taaaaatatc
961 ccactttatc caattttcgt ttgttgaact aatgggtgct ttagttgaag aataaaagac
1021 cacattaaaa aatgtggtct tttgtgtttt tttaaaggat ttgagcgtag cgaaaaatcc
1081 ttttctttct tatcttgata ataagggtaa ctattgccga tgataagctg tcaactagta
1141 tggataagaa atactcaata ggcttagcta tcggcacaaa tagcgtcgga tgggcggtga
1201 tcactgatga atataaggtt ccgtctaaaa agttcaaggt tctgggaaat acagaccgcc
1261 acagtatcaa aaaaaatctt ataggggctc ttttatttga cagtggagag acagcggaag
1321 cgactcgtct caaacggaca gctcgtagaa ggtatacacg tcggaagaat cgtatttgtt
1381 atctacagga gattttttca aatgagatgg cgaaagtaga tgatagtttc tttcatcgac
1441 ttgaagagtc ttttttggtg gaagaagaca agaagcatga acgtcatcct atttttggaa
1501 atatagtaga tgaagttgct tatcatgaga aatatccaac tatctatcat ctgcgaaaaa
1561 aattggtaga ttctactgat aaagcggatt tgcgcttaat ctatttggcc ttagcgcata
1621 tgattaagtt tcgtggtcat tttttgattg agggagattt aaatcctgat aatagtgatg
1681 tggacaaact atttatccag ttggtacaaa cctacaatca attatttgaa gaaaacccta
1741 ttaacgcaag tggagtagat gctaaagcga ttctttctgc acgattgagt aaatcaagac
1801 gattagaaaa tctcattgct cagctccccg gtgagaagaa aaatggctta tttgggaatc
1861 tcattgcttt gtcattgggt ttgaccccta attttaaatc aaattttgat ttggcagaag
1921 atgctaaatt acagctttca aaagatactt acgatgatga tttagataat ttattggcgc
1981 aaattggaga tcaatatgct gatttgtttt tggcagctaa gaatttatca gatgctattt
2041 tactttcaga tatcctaaga gtaaatactg aaataactaa ggctccccta tcagcttcaa
2101 tgattaaacg ctacgatgaa catcatcaag acttgactct tttaaaagct ttagttcgac
2161 aacaacttcc agaaaagtat aaagaaatct tttttgatca atcaaaaaac ggatatgcag
2221 gttatattga tgggggagct agccaagaag aattttataa atttatcaaa ccaattttag
2281 aaaaaatgga tggtactgag gaattattgg tgaaactaaa tcgtgaagat ttgctgcgca
2341 agcaacggac ctttgacaac ggctctattc cccatcaaat tcacttgggt gagctgcatg
2401 ctattttgag aagacaagaa gacttttatc catttttaaa agacaatcgt gagaagattg
2461 aaaaaatctt gacttttcga atcccttatt atgttggtcc attggcgcgt ggcaatagtc
2521 gttttgcatg gatgactcgg aagtctgaag aaacaattac cccatggaat tttgaagaag
2581 ttgtcgataa aggtgcttca gctcaatcat ttattgaacg catgacaaac tttgataaaa
2641 atcttccaaa tgaaaaagta ctaccaaaac atagtttgct ttatgagtat tttacggttt
2701 ataacgaatt gacaaaggtc aaatatgtta ctgaaggaat gcgaaaacca gcatttcttt
2761 caggtgaaca gaagaaagcc attgttgatt tactcttcaa aacaaatcga aaagtaaccg
2821 ttaagcaatt aaaagaagat tatttcaaaa aaatagaatg ttttgatagt gttgaaattt
2881 caggagttga agatagattt aatgcttcat taggtaccta ccatgatttg ctaaaaatta
2941 ttaaagataa agattttttg gataatgaag aaaatgaaga tatcttagag gatattgttt
3001 taacattgac cttatttgaa gatagggaga tgattgagga aagacttaaa acatatgctc
3061 acctctttga tgataaggtg atgaaacagc ttaaacgtcg ccgttatact ggttggggac
3121 gtttgtctcg aaaattgatt aatggtatta gggataagca atctggcaaa acaatattag
3181 attttttgaa atcagatggt tttgccaatc gcaattttat gcagctgatc catgatgata
3241 gtttgacatt taaagaagac attcaaaaag cacaagtgtc tggacaaggc gatagtttac
3301 atgaacatat tgcaaattta gctggtagcc ctgctattaa aaaaggtatt ttacagactg
3361 taaaagttgt tgatgaattg gtcaaagtaa tggggcggca taagccagaa aatatcgtta
3421 ttgaaatggc acgtgaaaat cagacaactc aaaagggcca gaaaaattcg cgagagcgta
3481 tgaaacgaat cgaagaaggt atcaaagaat taggaagtca gattcttaaa gagcatcctg
3541 ttgaaaatac tcaattgcaa aatgaaaagc tctatctcta ttatctccaa aatggaagag
3601 acatgtatgt ggaccaagaa ttagatatta atcgtttaag tgattatgat gtcgatgcca
3661 ttgttccaca aagtttcctt aaagacgatt caatagacaa taaggtctta acgcgttctg
3721 ataaaaatcg tggtaaatcg gataacgttc caagtgaaga agtagtcaaa aagatgaaaa
3781 actattggag acaacttcta aacgccaagt taatcactca acgtaagttt gataatttaa
3841 cgaaagctga acgtggaggt ttgagtgaac ttgataaagc tggttttatc aaacgccaat
3901 tggttgaaac tcgccaaatc actaagcatg tggcacaaat tttggatagt cgcatgaata
3961 ctaaatacga tgaaaatgat aaacttattc gagaggttaa agtgattacc ttaaaatcta
4021 aattagtttc tgacttccga aaagatttcc aattctataa agtacgtgag attaacaatt
4081 accatcatgc ccatgatgcg tatctaaatg ccgtcgttgg aactgctttg attaagaaat
4141 atccaaaact tgaatcggag tttgtctatg gtgattataa agtttatgat gttcgtaaaa
4201 tgattgctaa gtctgagcaa gaaataggca aagcaaccgc aaaatatttc ttttactcta
4261 atatcatgaa cttcttcaaa acagaaatta cacttgcaaa tggagagatt cgcaaacgcc
4321 ctctaatcga aactaatggg gaaactggag aaattgtctg ggataaaggg cgagattttg
4381 ccacagtgcg caaagtattg tccatgcccc aagtcaatat tgtcaagaaa acagaagtac
4441 agacaggcgg attctccaag gagtcaattt taccaaaaag aaattcggac aagcttattg
4501 ctcgtaaaaa agactgggat ccaaaaaaat atggtggttt tgatagtcca acggtagctt
4561 attcagtcct agtggttgct aaggtggaaa aagggaaatc gaagaagtta aaatccgtta
4621 aagagttact agggatcaca attatggaaa gaagttcctt tgaaaaaaat ccgattgact
4681 ttttagaagc taaaggatat aaggaagtta aaaaagactt aatcattaaa ctacctaaat
4741 atagtctttt tgagttagaa aacggtcgta aacggatgct ggctagtgcc ggagaattac
4801 aaaaaggaaa tgagctggct ctgccaagca aatatgtgaa ttttttatat ttagctagtc
4861 attatgaaaa gttgaagggt agtccagaag ataacgaaca aaaacaattg tttgtggagc
4921 agcataagca ttatttagat gagattattg agcaaatcag tgaattttct aagcgtgtta
4981 ttttagcaga tgccaattta gataaagttc ttagtgcata taacaaacat agagacaaac
5041 caatacgtga acaagcagaa aatattattc atttatttac gttgacgaat cttggagctc
5101 ccgctgcttt taaatatttt gatacaacaa ttgatcgtaa acgatatacg tctacaaaag
5161 aagttttaga tgccactctt atccatcaat ccatcactgg tctttatgaa acacgcattg
5221 atttgagtca gctaggaggt gacgcggccg cggagcagaa actcatctct gaagaagatc
5281 tggaacaaaa gttgatttca gaagaagatc tggaacagaa gctcatctct gaggaagatc
5341 tgtaataagg cgcgcctcct taatgggact tgcagcctcg gtaccaaatt ccagaaaaga
5401 cacccgaaag ggtgtttttt cgttttggtc ccacagaatg agcatcatgg ctctagtcga
5461 ccgggtaggg gcatagctta gataattgga aaagaggaaa aaagcttaat cttttttcga
5521 aggttaagct ttttctttta tttataaaaa gtgaactaac tatcagaaag aaattatatt
5581 aaattttatt tttttgttta aaaagtagat tatataaagg caagctaggt gggggaaaat
5641 atgtttaaaa aagaaaaagt cacagaatac atttggacta tactaatacc aacaatcatc
5701 acttttatca ttagttgggt tgggtcttat tacaatggta cttcgacagt tagtattgga
5761 caacctacaa aagtttccgg tcagtatatc acgccaataa atataagtcc ctatcatgat
5821 attaaggaat taagaataac ttttccgcaa aaactagatg taaaacaaat tagttcaaat
5881 gagcctataa atgtaaaatc agataagaac aatataggag ttgaaagtaa ttccactttt
5941 gagattgcga aaatcgttga aaataatagc gttcagttgc taattacaac acaaaaaaag
6001 ttaaacgata aggaaattag aattgataaa aatggaaata acatttctgt aaattatgaa
6061 tctcagattg ttaatcctgc aaaaaaacaa ttaatcaatc ttataattac gtcatctatt
6121 tattttataa tgcttaatat actagcattg attatgaaca aaagatggga taagtattat
6181 gcaaaaatga aaaatgaaat caaagaattt gaggataatg caaaagatct tgataaaaaa
6241 tcaaagaaga aaagcgagga attatcggag ctgcgaaaga ccttgaacca agcgtttgag
6301 gaaactgata ggataaaata tcatgagaag aaaaaacaaa tcctcctctt agctaagtta
6361 aacgattata aaaaagaact aaccttttgg agaaatacaa taagaaaagt tctttatgaa
6421 cttcctgatg gagataaaaa agcagataaa ctaataggga cagttacatc atctttaaaa
6481 acgtacggta cagtcgaaaa gtaaagctta tcggccagcc tcgcagagca ggattcccgt
6541 tgagcaccgc caggtgcgaa taagggacag tgaagaagga acacccgctc gcgggtgggc
6601 ctacttcacc tatcctgccc ggctgacgcc gttggataca ccaaggaaag tctacacgaa
6661 ccctttggca aaatcctgta tatcgtgcga aaaaggatgg atataccgaa aaaatcgcta
6721 taatgacccc gaagcagggt tatgcagcgg aaagtatacc ttaaggaatc ccctgataac
6781 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgca
6841 ctccctgcct ctgtcatcac gatactgtga tgccatggct aattcccatg tcagccgtta
6901 agtgttcctg tgtcactcaa aattgctttg agaggctcta agggcttctc agtgcgttac
6961 atccctggct tgttgtccac aaccgttaaa ccttaaaagc tttaaaagcc ttatatattc
7021 ttttttttct tataaaactt aaaaccttag aggctattta agttgctgat ttatattaat
7081 tttattgttc aaacatgaga gcttagtacg tgaaacatga gagcttagta cgttagccat
7141 gagagcttag tacgttagcc atgagggttt agttcgttaa acatgagagc ttagtacgtt
7201 aaacatgaga gcttagtacg tgaaacatga gagcttagta cgtactatca acaggttgaa
7261 ctgctgatct tcagatcctc tacgccggac gcatcgtggc cggatcttgc ggccgcaaaa
7321 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
7381 accaatgctt aatcagtgag gcaccaataa ctgcctttga tcttttctac ggggtctgac
7441 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
7501 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
7561 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
7621 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
7681 ggcttaccat ctggccccag tgctgcaatg ataccgcggg acccacgctc accggctcca
7741 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
7801 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
7861 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
7921 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
7981 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
8041 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
8101 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
8161 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
8221 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
8281 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
8341 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
8401 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
8461 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
8521 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtcttctaa
8581 ctacttattt taaagcagtc tggattgttt gggtaattca taaaaaaata aaagaaagaa
8641 ggaggaatag agtttttctt ttttttgttt gcaatgttac tgtcaagtcg caaaagaatt
8701 gctatgaccg aaaaaaaacc gcaaaggaat agatataagg tacctttttg caattcatct
8761 ttgtaaaata aaggttattc tgacataata caattaatgt aaaaattcgc acaattttat
8821 gtaaggatgg gggaattttc ttgcggggtg ttttcttaga taaagataaa attccgtacg
8881 acttagtcac gaaaaagtta aatgaatggt atacatcaat aaaaaatgat caagttgagc
8941 aagccgagat tataaaaaca gaagtagaga aagaattgtt aaacatggaa gaaaatcaag
9001 atgccctgtt atattatcaa ctattagaat ttagacatga gataatgctg agttatatga
9061 aatctaagga aatagaagat ctcaataatg cttatgagac tataaaagaa attgagaagc
9121 aagggcaatt aactggcatg ttggaatact atttttactt ttttaagggt atgtacgagt
9181 ttaggcgtaa agaattaatt tcagcgataa gtgcttatcg aatagctgaa tcaaagttgt
9241 cagaagttga ggatgaaata gagaaagcag agtttttttt caaagtgtcc tatgtatatt
9301 attatatgaa acaaacatac ttctccatga attatgcaaa tcgtgcactc aaaatattta
9361 gagagtatga agaatatgct gtccagactg tgcgttgtca atttattgta gcaggaaact
9421 tgatcgagtg aatcgataag ctagcttaat tagctgagct tggactcctg ttgatagatc
9481 cagtaatgac ctcagaactc catctggatt tgttcagaac gctcggttgc cgccgggcgt
9541 tttttattgg tgagaatcca agctagactg cgatgagtgg cagggcgggg cgtaattttt
9601 ttaaggcagt tattggtgcc cttaaacgcc tggggtaatg actctctagc ttgaggcatc
9661 aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg
9721 tgaacgctct cctgagtagg acaaatccgc cgctaggagc ttgcggcccg gacgatgagc
9781 tcgaattggg gatctt
//
