| 质粒名称: | pKC-4.04 |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 112085 |
| 存储实验室: | James Inglese |
|
载体骨架基本信息 |
|
| 载体名称: | p3xFLAG-CMV-10 |
| 载体出品公司: | -- |
| 空载体大小: | 6204 bp |
| 完整质粒大小: | 8717 bp |
| 载体修饰: | -- |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | 新霉素（G418） |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | 3XFLAG firefly luciferase-beta-globin,FLuc NMD sensor control |
| 物种来源: | H. sapiens (human), M. musculus (mouse); Photinus pyralis |
| 插入大小: | 2513 bp |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子: | CMV |
| 标签/融合蛋白: | FLAG（插入件上的N端子） |
| 克隆方法: | Gateway克隆 |
| 5’克隆位点: | -- |
| 3’克隆位点: | -- |
| 5’测序引物 | CMV-F |
| 3’测序引物 | hgH-PA-R |
|
文献引用方法 |
|
| 材料和方法部分: | pKC-4.04 was a gift from James Inglese (Addgene plasmid # 112085 ; http://n2t.net/addgene:112085 ; RRID:Addgene_112085) |
| 参考文献部分: | ICE1 promotes the link between splicing and nonsense-mediated mRNA decay. Baird TD, Cheng KC, Chen YC, Buehler E, Martin SE, Inglese J, Hogg JR. Elife. 2018 Mar 12;7. pii: 33178. doi: 10.7554/eLife.33178. 33178 [pii] PubMed 29528287 |
买家导航
pKC4.04载体基本信息
pKC4.04载体图谱质粒图谱和多克隆位点信息
pKC4.04载体简介
pKC4.04载体序列
LOCUS pKC-4.04 9274 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Control for NMD sensor: pCMV-3XFLAG-Fluc-betaglobin.
ACCESSION .
VERSION .
KEYWORDS pKC-4.04
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9274)
AUTHORS Baird TD, Cheng KC, Chen YC, Buehler E, Martin SE, Inglese J, Hogg
JR
TITLE ICE1 promotes the link between splicing and nonsense-mediated mRNA
decay.
JOURNAL Elife. 2018 Mar 12;7. pii: 33178. doi: 10.7554/eLife.33178.
PUBMED 29528287
REFERENCE 2 (bases 1 to 9274)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..9274
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 1..380
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 381..584
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 535..555
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
CDS 614..679
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
CDS 698..2344
/codon_start=1
/gene="luc+"
/product="firefly luciferase"
/label=luciferase
/note="enhanced luc+ version of the luciferase gene"
/translation="EDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDAH
IEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAPA
NDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQSM
YTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHAR
DPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYKI
QSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGYG
LTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMSG
YVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESILL
QHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVVF
VDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
primer_bind complement(761..779)
/label=LucNrev
/note="Firefly luciferase, reverse primer"
protein_bind 2372..2396
/gene="mutant version of attB"
/label=attB1
/bound_moiety="BP Clonase(TM)"
/note="recombination site for the Gateway(R) BP reaction"
protein_bind complement(3658..3682)
/gene="mutant version of attB"
/label=attB2
/bound_moiety="BP Clonase(TM)"
/note="recombination site for the Gateway(R) BP reaction"
polyA_signal 3716..4338
/label=hGH poly(A) signal
/note="human growth hormone polyadenylation signal"
primer_bind complement(3915..3934)
/label=hGH-PA-R
/note="Human growth hormone terminator, reverse primer"
primer_bind complement(4362..4382)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 4380..4677
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
primer_bind 4609..4628
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
CDS 4731..5525
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(4785..4804)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 5395..5414
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
polyA_signal 6177..6311
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(6214..6233)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 6268..6287
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind complement(6316..6335)
/label=T7
/note="T7 promoter, forward primer"
promoter complement(6317..6335)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(6349..6365)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(6349..6365)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(6362..6384)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 6373..6389
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(6397..6427)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 6442..6463
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(6580..6597)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(6751..7339)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(6831..6850)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(7510..8370)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 8133..8152
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(8371..8475)
/gene="bla"
/label=AmpR promoter
rep_origin 8502..8957
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(8589..8608)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 8799..8820
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind 9083..9105
/label=M13/pUC Forward
/note="In lacZ gene"
primer_bind 9097..9114
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind 9098..9114
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
ORIGIN
1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
241 aagtgtatca tatgccaagt ccgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
301 ggcattatgc ccagtacatg accttacggg actttcctac ttggcagtac atctacgtat
361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta caccaatggg cgtggatagc
421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaataa ccccgccccg ttgacgcaaa
541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc
601 agaattaacc atggactaca aagaccatga cggtgattat aaagatcatg acatcgatta
661 caaggatgac gatgacaagc ttgcggccgc gaattcagaa gacgccaaaa acataaagaa
721 aggcccggcg ccattctatc cgctggaaga tggaaccgct ggagagcaac tgcataaggc
781 tatgaagaga tacgccctgg ttcctggaac aattgctttt acagatgcac atatcgaggt
841 ggacatcact tacgctgagt acttcgaaat gtccgttcgg ttggcagaag ctatgaaacg
901 atatgggctg aatacaaatc acagaatcgt cgtatgcagt gaaaactctc ttcaattctt
961 tatgccggtg ttgggcgcgt tatttatcgg agttgcagtt gcgcccgcga acgacattta
1021 taatgaacgt gaattgctca acagtatggg catttcgcag cctaccgtgg tgttcgtttc
1081 caaaaagggg ttgcaaaaaa ttttgaacgt gcaaaaaaag ctcccaatca tccaaaaaat
1141 tattatcatg gattctaaaa cggattacca gggatttcag tcgatgtaca cgttcgtcac
1201 atctcatcta cctcccggtt ttaatgaata cgattttgtg ccagagtcct tcgataggga
1261 caagacaatt gcactgatca tgaactcctc tggatctact ggtctgccta aaggtgtcgc
1321 tctgcctcat agaactgcct gcgtgagatt ctcgcatgcc agagatccta tttttggcaa
1381 tcaaatcatt ccggatactg cgattttaag tgttgttcca ttccatcacg gttttggaat
1441 gtttactaca ctcggatatt tgatatgtgg atttcgagtc gtcttaatgt atagatttga
1501 agaagagctg tttctgagga gccttcagga ttacaagatt caaagtgcgc tgctggtgcc
1561 aaccctattc tccttcttcg ccaaaagcac tctgattgac aaatacgatt tatctaattt
1621 acacgaaatt gcttctggtg gcgctcccct ctctaaggaa gtcggggaag cggttgccaa
1681 gaggttccat ctgccaggta tcaggcaagg atatgggctc actgagacta catcagctat
1741 tctgattaca cccgaggggg atgataaacc gggcgcggtc ggtaaagttg ttccattttt
1801 tgaagcgaag gttgtggatc tggataccgg gaaaacgctg ggcgttaatc aaagaggcga
1861 actgtgtgtg agaggtccta tgattatgtc cggttatgta aacaatccgg aagcgaccaa
1921 cgccttgatt gacaaggatg gatggctaca ttctggagac atagcttact gggacgaaga
1981 cgaacacttc ttcatcgttg accgcctgaa gtctctgatt aagtacaaag gctatcaggt
2041 ggctcccgct gaattggaat ccatcttgct ccaacacccc aacatcttcg acgcaggtgt
2101 cgcaggtctt cccgacgatg acgccggtga acttcccgcc gccgttgttg ttttggagca
2161 cggaaagacg atgacggaaa aagagatcgt ggattacgtc gccagtcaag taacaaccgc
2221 gaaaaagttg cgcggaggag ttgtgtttgt ggacgaagta ccgaaaggtc ttaccggaaa
2281 actcgacgca agaaaaatca gagagatcct cataaaggcc aagaagggcg gaaagatcgc
2341 cgtggcgaat tcatcgatag atctgatatc aacaagtttg tacaaaaaag caggctccgc
2401 ggccgccccc ttcaccgtgc atctgactcc tgaggagaag tctgccgtta ctgccctgtg
2461 gggcaaggtg aacgtggatg aagttggtgg tgaggccctg ggcaggttgg tatcaaggtt
2521 acaagacagg tttaaggaga ccaatagaaa ctgggcatgt ggagacagag aagactcttg
2581 ggtttctgat aggcactgac tctctctgcc tattggtcta ttttcccacc cttaggctgc
2641 tggtggtcta cccttggacc cagaggttct ttgagtcctt tggggatctg tccactcctg
2701 atgctgttat gggcaaccct aaggtgaagg ctcatggcaa gaaagtgctc ggtgccttta
2761 gtgatggcct ggctcacctg gacaacctca agggcacctt tgccacactg agtgagctgc
2821 actgtgacaa gctgcacgtg gatcctgaga acttcagggt gagtctgatg ggcacctcct
2881 gggtttcctt cccctggcta ttctgctcaa ccttcctatc agaaaaaaag gggaagcgat
2941 tctagggagc agtctccatg actgtgtgtg gagtgttgac aagagttcgg atattttatt
3001 ctctactcag aattgctgct ccccctcact ctgttctgtg ttgtcatttc ctctttcttt
3061 ggtaagcttt taatttccag ttgcatttta ctaaattaat taagctggtt atttacttcc
3121 catcctgata tcagcttccc ctcctccttt cctcccagtc cttctctctc tcctctctct
3181 ttctctaatc ctttcctttc cctcagttca tttcttcttc tttgatctac gtttgtttgt
3241 ctttttaaat attgccttgt aacttgctca gaggacaagg aagatatgtc cctgtttctt
3301 ctcatagctc tcaagaatag tagcataatt ggcttttatg ccagggtgac aggggaagaa
3361 tatattttac atataaattc tgtttgacat aggattctta taataatttg tcagtagttt
3421 aaggttgcaa acaaatgtct ttgtaaataa gcctgcagta tctggtattt ttgctctaca
3481 gttatgttga tggttcttcc atattcccac agctcctggg caatatgatc gtgattgtgc
3541 tgggccacca cctgggcaag gatttcaccc ccgctgcaca ggctgccttc cagaaggtgg
3601 tggctggagt ggccactgcc ctggcccaca agtatcacta aaagggtggg cgcgccgacc
3661 cagctttctt gtacaaagtg gttgatatcg gtaccagtcg actctagagg atcccgggtg
3721 gcatccctgt gacccctccc cagtgcctct cctggccctg gaagttgcca ctccagtgcc
3781 caccagcctt gtcctaataa aattaagttg catcattttg tctgactagg tgtccttcta
3841 taatattatg gggtggaggg gggtggtatg gagcaagggg caagttggga agacaacctg
3901 tagggcctgc ggggtctatt gggaaccaag ctggagtgca gtggcacaat cttggctcac
3961 tgcaatctcc gcctcctggg ttcaagcgat tctcctgcct cagcctcccg agttgttggg
4021 attccaggca tgcatgacca ggctcagcta atttttgttt ttttggtaga gacggggttt
4081 caccatattg gccaggctgg tctccaactc ctaatctcag gtgatctacc caccttggcc
4141 tcccaaattg ctgggattac aggcgtgaac cactgctccc ttccctgtcc ttctgatttt
4201 aaaataacta taccagcagg aggacgtcca gacacagcat aggctacctg gccatgccca
4261 accggtggga catttgagtt gcttgcttgg cactgtcctc tcatgcgttg ggtccactca
4321 gtagatgcct gttgaattgg gtacgcggcc agcttggctg tggaatgtgt gtcagttagg
4381 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
4441 gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
4501 gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac
4561 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
4621 ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
4681 aattgatcag cttgggatct gatcaagaga caggatgagg atcgtttcgc atgattgaac
4741 aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact
4801 gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc
4861 gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg caggacgagg
4921 cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg
4981 tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt
5041 catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc
5101 atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag
5161 cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg
5221 ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc
5281 tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt
5341 ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg
5401 ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt
5461 acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct
5521 tctgagcggg actctggggt tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg
5581 agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga
5641 cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccccgg
5701 gctcgatccc ctcgcgagtt ggttcagctg ctgcctgagg ctggacgacc tcgcggagtt
5761 ctaccggcag tgcaaatccg tcggcatcca ggaaaccagc agcggctatc cgcgcatcca
5821 tgcccccgaa ctgcaggagt ggggaggcac gatggccgct ttggtcgacc cggacgggac
5881 gctcctgcgc ctgatacaga acgaattgct tgcaggcatc tcatgagtgt gtcttcccgt
5941 tttccgcctg aggtcactgc gtggatggag cgctggcgcc tgctgcgcga cggcgagctg
6001 ctcaccaccc actcgccaag ctggaaccgt aaaaaggccg cgttgctggc gtttttccat
6061 aggctccgcc gatcataatc agccatacca catttgtaga ggttttactt gctttaaaaa
6121 acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact
6181 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
6241 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
6301 atgtctggat caattcccta tagtgagtcg tattaaattc gtaatcatgt catagctgtt
6361 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
6421 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
6481 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
6541 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
6601 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
6661 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
6721 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
6781 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
6841 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
6901 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
6961 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
7021 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
7081 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
7141 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
7201 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
7261 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
7321 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
7381 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
7441 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
7501 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
7561 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
7621 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
7681 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
7741 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
7801 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
7861 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
7921 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
7981 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
8041 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
8101 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
8161 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
8221 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
8281 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
8341 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
8401 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
8461 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgcgccct gtagcggcgc
8521 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
8581 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
8641 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
8701 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt
8761 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
8821 aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc
8881 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat
8941 attaacgctt acaatttcca ttcgccattc aggctgcgca actgttggga agggcgatcg
9001 gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc aaggcgatta
9061 agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc cagtgccaag
9121 ctgatctata cattgaatca atattggcaa ttagccatat tagtcattgg ttatatagca
9181 taaatcaata ttggctattg gccattgcat acgttgtatc tatatcataa tatgtacatt
9241 tatattggct catgtccaat atgaccgcca tgtt
//
