| 质粒名称: | pKLV2-U6gRNA5(BbsI)-PGKpuro2AZsG-W |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 67975 |
| 存储实验室: | Kosuke Yusa |
|
载体骨架基本信息 |
|
| 载体名称: | pKLV2 lentiviral vector |
| 载体出品公司: | -- |
| 空载体大小: | 5783 bp |
| 完整质粒大小: | 8648 bp |
| 载体修饰: | -- |
| 载体类型: | 慢病毒载体 |
| 筛选标记: | -- |
| 载体抗性: | 氨苄霉素100 μg/mL |
| 生长温度: | 37 ℃ |
| 宿主菌: | Stbl3 |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | U6gRNA cassette, PGKpuro2AZsG cassette, WPRE |
| 物种来源: | H. sapiens (human), M. musculus (mouse) |
| 插入大小: | 2898 bp |
| 基因突变: | Deleted BbsI site within WPRE |
| GenBank编号 | -- |
| 启动子: | human U6 promoter, mouse PGK promoter |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | MluI(未破坏) |
| 3’克隆位点: | KpnI (未破坏) |
| 5’测序引物 | CATAATAGCAACAGACATAC |
| 3’测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pKLV2-U6gRNA5(BbsI)-PGKpuro2AZsG-W was a gift from Kosuke Yusa (Addgene plasmid # 67975 ; http://n2t.net/addgene:67975 ; RRID:Addgene_67975) |
| 参考文献部分: | A CRISPR Dropout Screen Identifies Genetic Vulnerabilities and Therapeutic Targets in Acute Myeloid Leukemia. Tzelepis K, Koike-Yusa H, De Braekeleer E, Li Y, Metzakopian E, Dovey OM, Mupo A, Grinkevich V, Li M, Mazan M, Gozdecka M, Ohnishi S, Cooper J, Patel M, McKerrell T, Chen B, Domingues AF, Gallipoli P, Teichmann S, Ponstingl H, McDermott U, Saez-Rodriguez J, Huntly BJ, Iorio F, Pina C, Vassiliou GS, Yusa K. Cell Rep. 2016 Oct 18;17(4):1193-1205. doi: 10.1016/j.celrep.2016.09.079. 10.1016/j.celrep.2016.09.079 PubMed 27760321 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
-PGKpuro2AZsG-W载体图谱.png)
载体简介
载体序列
LOCUS pKLV2-U6gRNA5(BbsI)-PGKpuro2AZsG-W 8648 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION CRISPR gRNA expression vector with an improved scaffold and puro/ZsG
markers.
ACCESSION .
VERSION .
KEYWORDS pKLV2-U6gRNA5(BbsI)-PGKpuro2AZsG-W
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8648)
AUTHORS Tzelepis K, Koike-Yusa H, De Braekeleer E, Li Y, Metzakopian E,
Dovey OM, Mupo A, Grinkevich V, Li M, Mazan M, Gozdecka M, Ohnishi
S, Cooper J, Patel M, McKerrell T, Chen B, Domingues AF, Gallipoli
P, Teichmann S, Ponstingl H, McDermott U, Saez-Rodriguez J, Huntly
BJ, Iorio F, Pina C, Vassiliou GS, Yusa K
TITLE A CRISPR Dropout Screen Identifies Genetic Vulnerabilities and
Therapeutic Targets in Acute Myeloid Leukemia.
JOURNAL Cell Rep. 2016 Oct 18;17(4):1193-1205. doi:
10.1016/j.celrep.2016.09.079.
PUBMED 27760321
REFERENCE 2 (bases 1 to 8648)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..8648
/organism="synthetic DNA construct"
/mol_type="other DNA"
rep_origin complement(3..458)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(140..161)
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind 352..371
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 585..607
/label=M13/pUC Forward
/note="In lacZ gene"
primer_bind 599..616
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind 600..616
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 626..645
/label=T7
/note="T7 promoter, forward primer"
promoter 626..644
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
promoter 960..1158
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 1113..1133
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
LTR 1176..1356
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
misc_feature 1403..1528
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 2027..2260
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
CDS 2445..2489
/codon_start=1
/product="antigenic peptide corresponding to amino acids
655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
et al., 2013)"
/label=gp41 peptide
/note="recognized by the 2H10 single-chain llama nanobody"
/translation="KNEQELLELDKWASL"
misc_feature 2787..2904
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
promoter 2979..3219
/label=U6 promoter
/note="RNA polymerase III promoter for human U6 snRNA"
primer_bind 2979..2999
/label=hU6-F
/note="Human U6 promoter, forward primer"
primer_bind 3150..3169
/label=LKO.1 5'
/note="Human U6 promoter, forward primer"
promoter 3354..3853
/label=PGK promoter
/note="mouse phosphoglycerate kinase 1 promoter"
primer_bind complement(3389..3412)
/label=MSCV-rev
/note="Murine stem cell virus, reverse primer"
primer_bind 3786..3805
/label=mPGK-F
/note="Mouse PGK promoter, forward primer"
CDS 3893..4489
/codon_start=1
/gene="pac from Streptomyces alboniger"
/product="puromycin N-acetyltransferase"
/label=PuroR
/note="confers resistance to puromycin"
/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
primer_bind complement(3893..3912)
/label=Puro-R
/note="Puromycin resistance gene, reverse primer. Also
called puro-variant-R"
primer_bind 4389..4409
/label=Puro-F
/note="Puromycin resistance gene, forward primer"
CDS 4508..4561
/codon_start=1
/product="2A peptide from Thosea asigna virus capsid
protein"
/label=T2A
/note="Eukaryotic ribosomes fail to insert a peptide bond
between the Gly and Pro residues, yielding separate
polypeptides."
/translation="EGRGSLLTCGDVEENPGP"
CDS 4568..5263
/codon_start=1
/product="Zoanthus green fluorescent protein"
/label=ZsGreen1
/note="mammalian codon-optimized"
/translation="MAQSKHGLTKEMTMKYRMEGCVDGHKFVITGEGIGYPFKGKQAIN
LCVVEGGPLPFAEDILSAAFMYGNRVFTEYPQDIVDYFKNSCPAGYTWDRSFLFEDGAV
CICNADITVSVEENCMYHESKFYGVNFPADGPVMKKMTDNWEPSCEKIIPVPKQGILKG
DVSMYLLLKDGGRLRCQFDTVYKAKSVPRKMPDWHFIQHKLTREDRSDAKNQKWHLTEH
AIASGSALP"
primer_bind complement(5323..5343)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(5741..5752)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
primer_bind complement(6204..6221)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 6210..6434
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
primer_bind complement(6460..6480)
/label=T3
/note="T3 promoter, forward primer"
promoter complement(6460..6478)
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
primer_bind complement(6499..6515)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(6499..6515)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(6512..6534)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 6523..6539
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(6547..6577)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 6592..6613
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(6730..6747)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(6901..7489)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(6981..7000)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(7660..8520)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 8283..8302
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(8521..8625)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tgactagtac
661 ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc
721 aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt
781 ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac
841 gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac
901 cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt
961 gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac ggggatttcc
1021 aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt
1081 tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg
1141 ggaggtctat ataagcagcg cgttttgcct gtactgggtc tctctggtta gaccagatct
1201 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
1261 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
1321 tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa
1381 agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg aagcgcgcac
1441 ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta gcggaggcta
1501 gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta gatcgcgatg
1561 ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac atatagtatg
1621 ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa catcagaagg
1681 ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag aagaacttag
1741 atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag agataaaaga
1801 caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga ccaccgcaca
1861 gcaagcggcc ggccgcgctg atcttcagac ctggaggagg agatatgagg gacaattgga
1921 gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta gcacccacca
1981 aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga gctttgttcc
2041 ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac
2101 aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg agggctattg
2161 aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc caggcaagaa
2221 tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg ggttgctctg
2281 gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat aaatctctgg
2341 aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac aattacacaa
2401 gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat gaacaagaat
2461 tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca aattggctgt
2521 ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga atagtttttg
2581 ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg tttcagaccc
2641 acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa ggtggagaga
2701 gagacagaga cagatccatt cgattagtga acggatcggc actgcgtgcg ccaattctgc
2761 agacaaatgg cagtattcat ccacaatttt aaaagaaaag gggggattgg ggggtacagt
2821 gcaggggaaa gaatagtaga cataatagca acagacatac aaactaaaga attacaaaaa
2881 caaattacaa aaattcaaaa ttttcgggtt tattacaggg acagcagaga tccagtttgg
2941 ttagtaccgg gccctacgcg tccaaggtcg ggcaggaaga gggcctattt cccatgattc
3001 cttcatattt gcatatacga tacaaggctg ttagagagat aattagaatt aatttgactg
3061 taaacacaaa gatattagta caaaatacgt gacgtagaaa gtaataattt cttgggtagt
3121 ttgcagtttt aaaattatgt tttaaaatgg actatcatat gcttaccgta acttgaaagt
3181 atttcgattt cttggcttta tatatcttgt ggaaaggacg aaacaccggg tcttcgagaa
3241 gacacgttta agagctatgc tggaaacagc atagcaagtt taaataaggc tagtccgtta
3301 tcaacttgaa aaagtggcac cgagtcggtg ctttttttgg atccaattct accgggtagg
3361 ggaggcgctt ttcccaaggc agtctggagc atgcgcttta gcagccccgc tgggcacttg
3421 gcgctacaca agtggcctct ggcctcgcac acattccaca tccaccggta ggcgccaacc
3481 ggctccgttc tttggtggcc ccttcgcgcc accttctact cctcccctag tcaggaagtt
3541 cccccccgcc ccgcagctcg cgtcgtgcag gacgtgacaa atggaagtag cacgtctcac
3601 tagtctcgtg cagatggaca gcaccgctga gcaatggaag cgggtaggcc tttggggcag
3661 cggccaatag cagctttgct ccttcgcttt ctgggctcag aggctgggaa ggggtgggtc
3721 cgggggcggg ctcaggggcg ggctcagggg cggggcgggc gcccgaaggt cctccggagg
3781 cccggcattc tgcacgcttc aaaagcgcac gtctgccgcg ctgttctcct cttcctcatc
3841 tccgggcctt tcgacctgca tccatctaga tctcgagcag ctgaagctta ccatgaccga
3901 gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc cccagggccg tacgcaccct
3961 cgccgccgcg ttcgccgact accccgccac gcgccacacc gtcgatccgg accgccacat
4021 cgagcgggtc accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa
4081 ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga
4141 agcgggggcg gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct
4201 ggccgcgcag caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg
4261 gttcctggcc accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt
4321 cgtgctcccc ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc
4381 cgcgccccgc aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga
4441 ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag cccggtgccg gcggcgggtc
4501 cggaggagag ggcagaggaa gtctcctaac atgcggtgac gtggaggaga atcctggccc
4561 agaattcatg gcccagtcca agcacggcct gaccaaggag atgaccatga agtaccgcat
4621 ggagggctgc gtggacggcc acaagttcgt gatcaccggc gagggcatcg gctacccctt
4681 caagggcaag caggccatca acctgtgcgt ggtggagggc ggccccttgc ccttcgccga
4741 ggacatcttg tccgccgcct tcatgtacgg caaccgcgtg ttcaccgagt acccccagga
4801 catcgtcgac tacttcaaga actcctgccc cgccggctac acctgggacc gctccttcct
4861 gttcgaggac ggcgccgtgt gcatctgcaa cgccgacatc accgtgagcg tggaggagaa
4921 ctgcatgtac cacgagtcca agttctacgg cgtgaacttc cccgccgacg gccccgtgat
4981 gaagaagatg accgacaact gggagccctc ctgcgagaag atcatccccg tgcccaagca
5041 gggcatcttg aagggcgacg tgagcatgta cctgctgctg aaggacggtg gccgcttgcg
5101 ctgccagttc gacaccgtgt acaaggccaa gtccgtgccc cgcaagatgc ccgactggca
5161 cttcatccag cacaagctga cccgcgagga ccgcagcgac gccaagaacc agaagtggca
5221 cctgaccgag cacgccatcg cctccggctc cgccttgccc taagcggccg ctcaacctct
5281 ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct
5341 atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat
5401 tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt
5461 caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat
5521 tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc
5581 ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga
5641 caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc
5701 cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga
5761 ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtctac gccttcgccc
5821 tcagacgagt cggatctccc tttgggccgc ctccccgcag gtacctttaa gaccaatgac
5881 ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct
5941 aattcactcc caaagaagtc aagatctgct ttttgcctgt actgggtctc tctggttaga
6001 ccagagtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
6061 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
6121 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
6181 agcagtttaa acccgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt
6241 ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta
6301 ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg
6361 ggtggggcag gacagcaagg gggaggattg ggaagtcaat agcaggcatg ctggggatgc
6421 ggtgggctct atgggcggcc gttaattaag cttttgttcc ctttagtgag ggttaattgc
6481 gcgcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
6541 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
6601 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
6661 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
6721 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
6781 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
6841 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
6901 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
6961 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
7021 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
7081 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
7141 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
7201 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
7261 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
7321 taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac
7381 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
7441 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
7501 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
7561 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
7621 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
7681 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
7741 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
7801 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
7861 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
7921 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
7981 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
8041 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
8101 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
8161 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
8221 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
8281 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
8341 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
8401 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
8461 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
8521 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
8581 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
8641 agtgccac
//
载体图谱质粒图谱pdf版和相关资料下载
载体质粒应用举例
- 上一篇：pLM-CMV-R-Cre
- 下一篇：Apple-53BP1trunc
