| 质粒名称: | pLSC-5 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 62889 |
| 存储实验室: | Feng Zhang |
|
载体骨架基本信息 |
|
| 载体名称: | lentiCRISPR |
| 载体出品公司: | Zhang lab |
| 载体抗性: | 氨苄霉素 |
| 空载体大小: | 8809 bp |
| 完整质粒大小: | 13792 bp |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | 嘌呤霉素 |
| 高拷贝/低拷贝: | 未知 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | SpCas9(574-1368) |
| 物种来源: | 光合细菌 Rhodopseudomonas palustris |
| 插入大小: | 2916 bp |
| 基因突变: | *see comment below |
| GenBank编号 | JN247409 |
| 启动子 | EFS |
| 标签/融合蛋白: | NLS SV40（N端） FFBP12（N端） P2A（C端） |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | Age1(未破坏) |
| 3' 克隆位点: | BamH1(未破坏) |
| 5' 测序引物 | NA |
| 3' 测序引物 | |
|
文献引用方法 |
|
| 材料和方法部分: | pLSC-5 was a gift from Feng Zhang (Addgene plasmid # 62889) |
| 参考文献部分: | A split-Cas9 architecture for inducible genome editing and transcription modulation. Zetsche B, Volz SE, Zhang F. Nat Biotechnol. 2015 Feb 2. doi: 10.1038/nbt.3149. 10.1038/nbt.3149 PubMed 25643054 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pLSC-5 13792 bp DNA SYN
DEFINITION pLSC-5
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
FEATURES Location/Qualifiers
source 1..13792
/organism="pLSC-5"
/mol_type="other DNA"
promoter 239..815
/label="CMV_immearly_promoter"
misc_feature 318..605
/label="CAG_enhancer"
misc_feature 772..792
/label="CMV_fwd_primer"
misc_feature 835..1015
/label="HIV-1_5_LTR"
misc_feature 835..1015
/label="truncHIV-1_3_LTR"
misc_feature 1126..1170
/label="HIV-1_psi_pack"
CDS 1558..2445
/label="ORF frame 1"
misc_feature 1680..1913
/label="RRE"
misc_feature 2444..2459
/label="cPPT"
promoter 2617..2849
/label="hU6_promoter"
misc_feature 2779..2798
/label="LKO1_5_primer"
CDS complement(2962..4452)
/label="ORF frame 2"
CDS 3235..8868
/label="ORF frame 1"
misc_feature 3241..3261
/label="SV40 NLS"
misc_feature 3241..3264
/label="SV40 NLS"
misc_feature 3247..3261
/label="NLS"
gene 8281..8868
/label="puro (variant)"
/gene="puro (variant)"
misc_feature 8886..9461
/label="WPRE"
CDS 8974..9483
/label="ORF frame 1"
CDS 8987..9517
/label="ORF frame 2"
misc_feature 9527..9542
/label="cPPT"
misc_feature 9527..9548
/label="U3PPT"
misc_feature 9544..9596
/label="delta_U3"
misc_feature 9597..9777
/label="truncHIV-1_3_LTR"
misc_feature 9597..9777
/label="HIV-1_5_LTR"
misc_feature complement(9803..9820)
/label="BGH_rev_primer"
terminator 9806..10033
/label="bGH_PA_terminator"
rep_origin 10096..10402
/label="f1_origin"
misc_feature complement(10516..10536)
/label="pBABE_3_primer"
misc_feature complement(10522..10737)
/label="SV40_enhancer"
promoter 10534..10802
/label="SV40_promoter"
rep_origin 10701..10778
/label="SV40_origin"
misc_feature 10763..10782
/label="SV40pro_F_primer"
promoter 10896..10963
/label="EM7_promoter"
misc_feature 10964..11338
/label="sh_ble"
gene 10964..11335
/label="bleo"
/gene="bleo"
terminator 11471..11590
/label="SV40_PA_terminator"
misc_feature 11559..11578
/label="EBV_rev_primer"
promoter complement(11634..11652)
/label="M13_reverse_primer"
misc_feature complement(11651..11673)
/label="M13_pUC_rev_primer"
promoter complement(11687..11716)
/label="lac_promoter"
gene complement(12799..13659)
/label="Ampicillin"
/gene="Ampicillin"
CDS complement(12799..13659)
/label="ORF frame 2"
promoter complement(13701..13729)
/label="AmpR_promoter"
ORIGIN
1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
2581 agatccagtt tggttaatta aggtaccgag ggcctatttc ccatgattcc ttcatatttg
2641 catatacgat acaaggctgt tagagagata attagaatta atttgactgt aaacacaaag
2701 atattagtac aaaatacgtg acgtagaaag taataatttc ttgggtagtt tgcagtttta
2761 aaattatgtt ttaaaatgga ctatcatatg cttaccgtaa cttgaaagta tttcgatttc
2821 ttggctttat atatcttGTG GAAAGGACGA AACACCgGAG ACGgaCGTCT Ctgttttaga
2881 gctaGAAAta gcaagttaaa ataaggctag tccgttatca acttgaaaaa gtggcaccga
2941 gtcggtgcTT TTTTgaattc gctagctagg tcttgaaagg agtgggaatt ggctccggtg
3001 cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg ggaggggtcg
3061 gcaattgatc cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt
3121 actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca gtagtcgccg
3181 tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggaccggtgc caccATGGCC
3241 CCAAAGAAGA AGCGGAAGGT CGGTATCCAC GGAGTCCCAG CAGCCGGCGT GCAGGTGGAG
3301 ACCATCAGCC CCGGCGACGG CAGAACCTTC CCCAAGAGAG GCCAGACCTG CGTGGTGCAC
3361 TACACCGGCA TGCTGGAGGA CGGCAAGAAG TTCGACAGCA GCAGAGACAG AAACAAGCCC
3421 TTCAAGTTCA TGCTGGGCAA GCAGGAGGTG ATCAGAGGCT GGGAGGAGGG CGTGGCCCAG
3481 ATGAGCGTGG GCCAGAGAGC CAAGCTGACC ATCAGCCCCG ACTACGCCTA CGGCGCCACC
3541 GGCCACCCCG GCATCATCCC TCCTCACGCC ACCCTGGTGT TCGACGTGGA GCTGCTGAAG
3601 CTGGAGGGTG GCGGTGGCtc gGGTGGCGGT GGCtcgGGTG GCGGTGGCtc gTGCTTCGAC
3661 TCCGTGGAAA TCTCCGGCGT GGAAGATCGG TTCAACGCCT CCCTGGGCGC ATACCACGAT
3721 CTGCTGAAAA TTATCAAGGA CAAGGACTTC CTGGACAATG AGGAAAACGA GGACATTCTG
3781 GAAGATATCG TGCTGACCCT GACACTGTTT GAGGACAGAG AGATGATCGA GGAACGGCTG
3841 AAAACCTATG CCCACCTGTT CGACGACAAA GTGATGAAGC AGCTGAAGCG GCGGAGATAC
3901 ACCGGCTGGG GCAGGCTGAG CCGGAAGCTG ATCAACGGCA TCCGGGACAA GCAGTCCGGC
3961 AAGACAATCC TGGATTTCCT GAAGTCCGAC GGCTTCGCCA ACAGAAACTT CATGCAGCTG
4021 ATCCACGACG ACAGCCTGAC CTTTAAAGAG GACATCCAGA AAGCCCAGGT GTCCGGCCAG
4081 GGCGATAGCC TGCACGAGCA CATTGCCAAT CTGGCCGGCA GCCCCGCCAT TAAGAAGGGC
4141 ATCCTGCAGA CAGTGAAGGT GGTGGACGAG CTCGTGAAAG TGATGGGCCG GCACAAGCCC
4201 GAGAACATCG TGATCGAAAT GGCCAGAGAG AACCAGACCA CCCAGAAGGG ACAGAAGAAC
4261 AGCCGCGAGA GAATGAAGCG GATCGAAGAG GGCATCAAAG AGCTGGGCAG CCAGATCCTG
4321 AAAGAACACC CCGTGGAAAA CACCCAGCTG CAGAACGAGA AGCTGTACCT GTACTACCTG
4381 CAGAATGGGC GGGATATGTA CGTGGACCAG GAACTGGACA TCAACCGGCT GTCCGACTAC
4441 GATGTGGACC ATATCGTGCC TCAGAGCTTT CTGAAGGACG ACTCCATCGA CAACAAGGTG
4501 CTGACCAGAA GCGACAAGAA CCGGGGCAAG AGCGACAACG TGCCCTCCGA AGAGGTCGTG
4561 AAGAAGATGA AGAACTACTG GCGGCAGCTG CTGAACGCCA AGCTGATTAC CCAGAGAAAG
4621 TTCGACAATC TGACCAAGGC CGAGAGAGGC GGCCTGAGCG AACTGGATAA GGCCGGCTTC
4681 ATCAAGAGAC AGCTGGTGGA AACCCGGCAG ATCACAAAGC ACGTGGCACA GATCCTGGAC
4741 TCCCGGATGA ACACTAAGTA CGACGAGAAT GACAAGCTGA TCCGGGAAGT GAAAGTGATC
4801 ACCCTGAAGT CCAAGCTGGT GTCCGATTTC CGGAAGGATT TCCAGTTTTA CAAAGTGCGC
4861 GAGATCAACA ACTACCACCA CGCCCACGAC GCCTACCTGA ACGCCGTCGT GGGAACCGCC
4921 CTGATCAAAA AGTACCCTAA GCTGGAAAGC GAGTTCGTGT ACGGCGACTA CAAGGTGTAC
4981 GACGTGCGGA AGATGATCGC CAAGAGCGAG CAGGAAATCG GCAAGGCTAC CGCCAAGTAC
5041 TTCTTCTACA GCAACATCAT GAACTTTTTC AAGACCGAGA TTACCCTGGC CAACGGCGAG
5101 ATCCGGAAGC GGCCTCTGAT CGAGACAAAC GGCGAAACCG GGGAGATCGT GTGGGATAAG
5161 GGCCGGGATT TTGCCACCGT GCGGAAAGTG CTGAGCATGC CCCAAGTGAA TATCGTGAAA
5221 AAGACCGAGG TGCAGACAGG CGGCTTCAGC AAAGAGTCTA TCCTGCCCAA GAGGAACAGC
5281 GATAAGCTGA TCGCCAGAAA GAAGGACTGG GACCCTAAGA AGTACGGCGG CTTCGACAGC
5341 CCCACCGTGG CCTATTCTGT GCTGGTGGTG GCCAAAGTGG AAAAGGGCAA GTCCAAGAAA
5401 CTGAAGAGTG TGAAAGAGCT GCTGGGGATC ACCATCATGG AAAGAAGCAG CTTCGAGAAG
5461 AATCCCATCG ACTTTCTGGA AGCCAAGGGC TACAAAGAAG TGAAAAAGGA CCTGATCATC
5521 AAGCTGCCTA AGTACTCCCT GTTCGAGCTG GAAAACGGCC GGAAGAGAAT GCTGGCCTCT
5581 GCCGGCGAAC TGCAGAAGGG AAACGAACTG GCCCTGCCCT CCAAATATGT GAACTTCCTG
5641 TACCTGGCCA GCCACTATGA GAAGCTGAAG GGCTCCCCCG AGGATAATGA GCAGAAACAG
5701 CTGTTTGTGG AACAGCACAA GCACTACCTG GACGAGATCA TCGAGCAGAT CAGCGAGTTC
5761 TCCAAGAGAG TGATCCTGGC CGACGCTAAT CTGGACAAAG TGCTGTCCGC CTACAACAAG
5821 CACCGGGATA AGCCCATCAG AGAGCAGGCC GAGAATATCA TCCACCTGTT TACCCTGACC
5881 AATCTGGGAG CCCCTGCCGC CTTCAAGTAC TTTGACACCA CCATCGACCG GAAGAGGTAC
5941 ACCAGCACCA AAGAGGTGCT GGATGCCACC CTGATCCACC AGAGCATCAC CGGCCTGTAC
6001 GAGACACGGA TCGACCTGTC TCAGCTGGGA GGCGACAAAA GGCCGGCGGC CACGAAAAAG
6061 GCCGGCCAGG CAAAAAAGAA AAAGGGATCC GGCGCAACAA ACTTCTCTCT GCTGAAACAA
6121 GCCGGAGATG TCGAAGAGAA TCCTGGACCG CTAGATTTAG CTAGCTTAAT CCTCGACAAG
6181 AAGTACAGCA TCGGCCTGGA CATCGGCACC AACTCTGTGG GCTGGGCCGT GATCACCGAC
6241 GAGTACAAGG TGCCCAGCAA GAAATTCAAG GTGCTGGGCA ACACCGACCG GCACAGCATC
6301 AAGAAGAACC TGATCGGAGC CCTGCTGTTC GACAGCGGCG AAACAGCCGA GGCCACCCGG
6361 CTGAAGAGAA CCGCCAGAAG AAGATACACC AGACGGAAGA ACCGGATCTG CTATCTGCAA
6421 GAGATCTTCA GCAACGAGAT GGCCAAGGTG GACGACAGCT TCTTCCACAG ACTGGAAGAG
6481 TCCTTCCTGG TGGAAGAGGA TAAGAAGCAC GAGCGGCACC CCATCTTCGG CAACATCGTG
6541 GACGAGGTGG CCTACCACGA GAAGTACCCC ACCATCTACC ACCTGAGAAA GAAACTGGTG
6601 GACAGCACCG ACAAGGCCGA CCTGCGGCTG ATCTATCTGG CCCTGGCCCA CATGATCAAG
6661 TTCCGGGGCC ACTTCCTGAT CGAGGGCGAC CTGAACCCCG ACAACAGCGA CGTGGACAAG
6721 CTGTTCATCC AGCTGGTGCA GACCTACAAC CAGCTGTTCG AGGAAAACCC CATCAACGCC
6781 AGCGGCGTGG ACGCCAAGGC CATCCTGTCT GCCAGACTGA GCAAGAGCAG ACGGCTGGAA
6841 AATCTGATCG CCCAGCTGCC CGGCGAGAAG AAGAATGGCC TGTTCGGAAA CCTGATTGCC
6901 CTGAGCCTGG GCCTGACCCC CAACTTCAAG AGCAACTTCG ACCTGGCCGA GGATGCCAAA
6961 CTGCAGCTGA GCAAGGACAC CTACGACGAC GACCTGGACA ACCTGCTGGC CCAGATCGGC
7021 GACCAGTACG CCGACCTGTT TCTGGCCGCC AAGAACCTGT CCGACGCCAT CCTGCTGAGC
7081 GACATCCTGA GAGTGAACAC CGAGATCACC AAGGCCCCCC TGAGCGCCTC TATGATCAAG
7141 AGATACGACG AGCACCACCA GGACCTGACC CTGCTGAAAG CTCTCGTGCG GCAGCAGCTG
7201 CCTGAGAAGT ACAAAGAGAT TTTCTTCGAC CAGAGCAAGA ACGGCTACGC CGGCTACATT
7261 GACGGCGGAG CCAGCCAGGA AGAGTTCTAC AAGTTCATCA AGCCCATCCT GGAAAAGATG
7321 GACGGCACCG AGGAACTGCT CGTGAAGCTG AACAGAGAGG ACCTGCTACG GAAGCAGCGG
7381 ACCTTCGACA ACGGCAGCAT CCCCCACCAG ATCCACCTGG GAGAGCTGCA CGCCATTCTG
7441 CGGCGGCAGG AAGATTTTTA CCCATTCCTG AAGGACAACC GGGAAAAGAT CGAGAAGATC
7501 CTGACCTTCC GCATCCCCTA CTACGTGGGC CCTCTGGCCA GGGGAAACAG CAGATTCGCC
7561 TGGATGACCA GAAAGAGCGA GGAAACCATC ACCCCCTGGA ACTTCGAGGA AGTGGTGGAC
7621 AAGGGCGCTT CCGCCCAGAG CTTCATCGAG CGGATGACCA ACTTCGATAA GAACCTGCCC
7681 AACGAGAAGG TGCTGCCCAA GCACAGCCTG CTGTACGAGT ACTTCACCGT GTATAACGAG
7741 CTGACCAAAG TGAAATACGT GACCGAGGGA ATGAGAAAGC CCGCCTTCCT GAGCGGCGAG
7801 CAGAAAAAGG CCATCGTGGA CCTGCTGTTC AAGACCAACC GGAAAGTGAC CGTGAAGCAG
7861 CTGAAAGAGG ACTACTTCAA GAAAATCGAG GGTGGCGGTG GCtcgGGTGG CGGTGGCtcg
7921 GGTGGCGGTG GCtcgGAGAT GTGGCACGAG GGCCTGGAGG AGGCCAGCAG ACTGTACTTC
7981 GGCGAGAGAA ACGTGAAGGG CATGTTCGAG GTGCTGGAGC CCCTGCACGC CATGATGGAG
8041 AGAGGCCCCC AGACCCTGAA GGAGACCAGC TTCAACCAGG CCTACGGCAG AGACCTGATG
8101 GAGGCCCAGG AGTGGTGCAG AAAGTACATG AAGAGCGGCA ACGTGAAGGA CCTGACCCAG
8161 GCCTGGGACC TGTACTACCA CGTGTTCAGA AGAATCAGCA AGCAGGGATC CGGCGCAACA
8221 AACTTCTCTC TGCTGAAACA AGCCGGAGAT GTCGAAGAGA ATCCTGGACC GACCGAGTAC
8281 AAGCCCACGG TGCGCCTCGC CACCCGCGAC GACGTCCCCA GGGCCGTACG CACCCTCGCC
8341 GCCGCGTTCG CCGACTACCC CGCCACGCGC CACACCGTCG ATCCGGACCG CCACATCGAG
8401 CGGGTCACCG AGCTGCAAGA ACTCTTCCTC ACGCGCGTCG GGCTCGACAT CGGCAAGGTG
8461 TGGGTCGCGG ACGACGGCGC CGCGGTGGCG GTCTGGACCA CGCCGGAGAG CGTCGAAGCG
8521 GGGGCGGTGT TCGCCGAGAT CGGCCCGCGC ATGGCCGAGT TGAGCGGTTC CCGGCTGGCC
8581 GCGCAGCAAC AGATGGAAGG CCTCCTGGCG CCGCACCGGC CCAAGGAGCC CGCGTGGTTC
8641 CTGGCCACCG TCGGAGTCTC GCCCGACCAC CAGGGCAAGG GTCTGGGCAG CGCCGTCGTG
8701 CTCCCCGGAG TGGAGGCGGC CGAGCGCGCC GGGGTGCCCG CCTTCCTGGA GACCTCCGCG
8761 CCCCGCAACC TCCCCTTCTA CGAGCGGCTC GGCTTCACCG TCACCGCCGA CGTCGAGGTG
8821 CCCGAAGGAC CGCGCACCTG GTGCATGACC CGCAAGCCCG GTGCCTGAAC GCGTTAAGTC
8881 GACAATCAAC CTCTGGATTA CAAAATTTGT GAAAGATTGA CTGGTATTCT TAACTATGTT
8941 GCTCCTTTTA CGCTATGTGG ATACGCTGCT TTAATGCCTT TGTATCATGC TATTGCTTCC
9001 CGTATGGCTT TCATTTTCTC CTCCTTGTAT AAATCCTGGT TGCTGTCTCT TTATGAGGAG
9061 TTGTGGCCCG TTGTCAGGCA ACGTGGCGTG GTGTGCACTG TGTTTGCTGA CGCAACCCCC
9121 ACTGGTTGGG GCATTGCCAC CACCTGTCAG CTCCTTTCCG GGACTTTCGC TTTCCCCCTC
9181 CCTATTGCCA CGGCGGAACT CATCGCCGCC TGCCTTGCCC GCTGCTGGAC AGGGGCTCGG
9241 CTGTTGGGCA CTGACAATTC CGTGGTGTTG TCGGGGAAAT CATCGTCCTT TCCTTGGCTG
9301 CTCGCCTGTG TTGCCACCTG GATTCTGCGC GGGACGTCCT TCTGCTACGT CCCTTCGGCC
9361 CTCAATCCAG CGGACCTTCC TTCCCGCGGC CTGCTGCCGG CTCTGCGGCC TCTTCCGCGT
9421 CTTCGCCTTC GCCCTCAGAC GAGTCGGATC TCCCTTTGGG CCGCCTCCCC GCGTCGACTT
9481 TAAGACCAAT GACTTACAAG GCAGCTGTAG ATCTTAGCCA CTTTTTAAAA GAAAAGGGGG
9541 GACTGGAAGG GCTAATTCAC TCCCAACGAA GACAAGATCT GCTTTTTGCT TGTACTGGGT
9601 CTCTCTGGTT AGACCAGATC TGAGCCTGGG AGCTCTCTGG CTAACTAGGG AACCCACTGC
9661 TTAAGCCTCA ATAAAGCTTG CCTTGAGTGC TTCAAGTAGT GTGTGCCCGT CTGTTGTGTG
9721 ACTCTGGTAA CTAGAGATCC CTCAGACCCT TTTAGTCAGT GTGGAAAATC TCTAGCAGgg
9781 cccgtttaaa cccgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt
9841 tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa
9901 taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg
9961 gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg
10021 gtgggctcta tggcttctga ggcggaaaga accagctggg gctctagggg gtatccccac
10081 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
10141 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
10201 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
10261 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca
10321 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
10381 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa
10441 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
10501 gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag
10561 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc
10621 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag
10681 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
10741 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctct gcctctgagc
10801 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctcccgg
10861 gagcttgtat atccattttc ggatctgatc agcacgtgtt gacaattaat catcggcata
10921 gtatatcggc atagtataat acgacaaggt gaggaactaa accatggcca agttgaccag
10981 tgccgttccg gtgctcaccg cgcgcgacgt cgccggagcg gtcgagttct ggaccgaccg
11041 gctcgggttc tcccgggact tcgtggagga cgacttcgcc ggtgtggtcc gggacgacgt
11101 gaccctgttc atcagcgcgg tccaggacca ggtggtgccg gacaacaccc tggcctgggt
11161 gtgggtgcgc ggcctggacg agctgtacgc cgagtggtcg gaggtcgtgt ccacgaactt
11221 ccgggacgcc tccgggccgg ccatgaccga gatcggcgag cagccgtggg ggcgggagtt
11281 cgccctgcgc gacccggccg gcaactgcgt gcacttcgtg gccgaggagc aggactgaca
11341 cgtgctacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt
11401 tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc
11461 ccaccccaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
11521 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
11581 tgtatcttat catgtctgta taccgtcgac ctctagctag agcttggcgt aatcatggtc
11641 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
11701 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
11761 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
11821 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
11881 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
11941 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
12001 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
12061 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
12121 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
12181 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
12241 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
12301 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
12361 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
12421 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
12481 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
12541 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
12601 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
12661 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
12721 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
12781 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
12841 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
12901 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
12961 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
13021 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
13081 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
13141 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
13201 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
13261 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
13321 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
13381 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
13441 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
13501 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
13561 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
13621 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
13681 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
13741 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg ac
//
