| 质粒名称: | pLenti6-V5-p53_R273H |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 22934 |
| 存储实验室: | Vladislav Verkhusha |
|
载体骨架基本信息 |
|
| 载体名称: | pLenti6/V5-D-Topo |
| 载体抗性: | 氨苄西林 |
| 空载体大小: | 6963bp |
| 载体类型: | 哺乳动物表达，慢病毒 |
| 筛选标记: | 杀菌剂 |
| 生长条件: | 37 ℃ |
| 宿生菌： | Stbl3 |
|
插入基因信息 |
|
| 插入基因名称: | tumor protein 53 |
| 备用名称： | TP53 |
| 物种来源: | H. sapiens (human) |
| 插入大小: | 1179 bp |
| 基因突变: | r273h突变体 |
| 标签/融合蛋白: | V5 (C terminal on backbone) |
| 克隆方法: | 拓扑克隆 |
| 5' 测序引物 | CMV |
|
文献引用方法 |
|
| 材料和方法部分: | |
| 参考文献部分: | Different mutant/wild-type p53 combinations cause a spectrum of increased invasive potential in nonmalignant immortalized human mammary epithelial cells. Junk DJ, Vrba L, Watts GS, Oshiro MM, Martinez JD, Futscher BW. Neoplasia. 2008 May . 10(5):450-61. PubMed 18472962 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pLenti6-V5-p53_R273H 8142 bp ds-DNA circular SYN 31-JUL-2019
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pLenti6/V5-p53_R273H
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8142)
AUTHORS Junk DJ, Vrba L, Watts GS, Oshiro MM, Martinez JD, Futscher BW
TITLE Different mutant/wild-type p53 combinations cause a spectrum of
increased invasive potential in nonmalignant immortalized human
mammary epithelial cells.
JOURNAL Neoplasia. 2008 May . 10(5):450-61.
PUBMED 18472962
REFERENCE 2 (bases 1 to 8142)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Jul 31, 2019 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..8142
/organism="synthetic DNA construct"
/mol_type="other DNA"
protein_bind 107..128
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 143..173
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 181..197
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 186..208
/label=M13/pUC Reverse
/note="In lacZ gene"
primer_bind 205..221
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 205..221
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind 240..260
/label=T3
/note="T3 promoter, forward primer"
promoter 242..260
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
promoter 288..514
/label=RSV promoter
/note="Rous sarcoma virus enhancer/promoter"
LTR 515..695
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
misc_feature 742..867
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 1360..1593
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
enhancer 2101..2404
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 2405..2608
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 2559..2579
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind 2605..2629
/label=LNCX
/note="Human CMV promoter, forward primer"
CDS 3937..3978
/codon_start=1
/product="epitope tag from simian virus 5"
/label=V5 tag
/translation="GKPIPNPLLGLDST"
primer_bind complement(4015..4035)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 4020..4349
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 4200..4335
/label=SV40 ori
/note="SV40 origin of replication"
promoter 4397..4444
/label=EM7 promoter
/note="synthetic bacterial promoter "
CDS 4463..4861
/codon_start=1
/gene="Aspergillus terreus BSD"
/product="blasticidin S deaminase"
/label=BSD
/note="confers resistance to blasticidin"
/translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
KAIVKDSDGQPTAVGIRELLPSGYVWEG"
LTR 4948..5181
/label=3' LTR (Delta-U3)
/note="self-inactivating 3' long terminal repeat (LTR) from
HIV-1"
polyA_signal 5253..5374
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(5290..5309)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 5344..5363
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
rep_origin 5414..5549
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind complement(5569..5588)
/label=T7
/note="T7 promoter, forward primer"
promoter complement(5570..5588)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(5598..5615)
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind complement(5598..5614)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(5607..5629)
/label=M13/pUC Forward
/note="In lacZ gene"
rep_origin 5756..6211
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(5843..5862)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 6053..6074
/label=F1ori-F
/note="F1 origin, forward primer"
promoter 6237..6341
/gene="bla"
/label=AmpR promoter
CDS 6342..7202
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(6560..6579)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 7373..7961
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 7862..7881
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 8115..8132
/label=L4440
/note="L4440 vector, forward primer"
ORIGIN
1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg
241 caattaaccc tcactaaagg gaacaaaagc tggagctgca agcttaatgt agtcttatgc
301 aatactcttg tagtcttgca acatggtaac gatgagttag caacatgcct tacaaggaga
361 gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg tacgatcgtg ccttattagg
421 aaggcaacag acgggtctga catggattgg acgaaccact gaattgccgc attgcagaga
481 tattgtattt aagtgcctag ctcgatacat aaacgggtct ctctggttag accagatctg
541 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
601 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
661 cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa
721 gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg
781 gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag
841 aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg
901 gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg
961 gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc
1021 tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga
1081 tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac
1141 accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag
1201 caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga
1261 attatataaa tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa
1321 gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt
1381 cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag
1441 acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca
1501 acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc
1561 tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact
1621 catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat
1681 ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat
1741 acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga
1801 attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat
1861 aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact
1921 ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc
1981 aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag
2041 agacagatcc attcgattag tgaacggatc tcgacggtat cgataagctt gggagttccg
2101 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
2161 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
2221 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
2281 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
2341 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
2401 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
2461 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
2521 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
2581 acggtgggag gtctatataa gcagagctcg tttagtgaac cgtcagatcg cctggagacg
2641 ccatccacgc tgttttgacc tccatagaag acaccgactc tagaggatcc actagtccag
2701 tgtggtggaa ttgatccctt caccatggag gagccgcagt cagatcctag cgtcgagccc
2761 cctctgagtc aggaaacatt ttcagaccta tggaaactac ttcctgaaaa caacgttctg
2821 tcccccttgc cgtcccaagc aatggatgat ttgatgctgt ccccggacga tattgaacaa
2881 tggttcactg aagacccagg tccagatgaa gctcccagaa tgccagaggc tgctcccccc
2941 gtggcccctg caccagcagc tcctacaccg gcggcccctg caccagcccc ctcctggccc
3001 ctgtcatctt ctgtcccttc ccagaaaacc taccagggca gctacggttt ccgtctgggc
3061 ttcttgcatt ctgggacagc caagtctgtg acttgcacgt actcccctgc cctcaacaag
3121 atgttttgcc aactggccaa gacctgccct gtgcagctgt gggttgattc cacacccccg
3181 cccggcaccc gcgtccgcgc catggccatc tacaagcagt cacagcacat gacggaggtt
3241 gtgaggcgct gcccccacca tgagcgctgc tcagatagcg atggtctggc ccctcctcag
3301 catcttatcc gagtggaagg aaatttgcgt gtggagtatt tggatgacag aaacactttt
3361 cgacatagtg tggtggtgcc ctatgagccg cctgaggttg gctctgactg taccaccatc
3421 cactacaact acatgtgtaa cagttcctgc atgggcggca tgaaccggag gcccatcctc
3481 accatcatca cactggaaga ctccagtggt aatctactgg gacggaacag ctttgaggtg
3541 catgtttgtg cctgtcctgg gagagaccgg cgcacagagg aagagaatct ccgcaagaaa
3601 ggggagcctc accacgagct gcccccaggg agcactaagc gagcactgcc caacaacacc
3661 agctcctctc cccagccaaa gaagaaacca ctggatggag aatatttcac ccttcagatc
3721 cgtgggcgtg agcgcttcga gatgttccga gagctgaatg aggccttgga actcaaggat
3781 gcccaggctg ggaaggagcc aggggggagc agggctcact ccagccacct gaagtccaaa
3841 aagggtcagt ctacctcccg ccataaaaaa ctcatgttca agacagaagg gcctgactca
3901 gacaagggct cgagtctaga gggcccgcgg ttcgaaggta agcctatccc taaccctctc
3961 ctcggtctcg attctacgcg taccggttag taatgagttt ggaattaatt ctgtggaatg
4021 tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
4081 tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa
4141 gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta actccgccca
4201 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
4261 ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag tagtgaggag
4321 gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata tccattttcg
4381 gatctgatca gcacgtgttg acaattaatc atcggcatag tatatcggca tagtataata
4441 cgacaaggtg aggaactaaa ccatggccaa gcctttgtct caagaagaat ccaccctcat
4501 tgaaagagca acggctacaa tcaacagcat ccccatctct gaagactaca gcgtcgccag
4561 cgcagctctc tctagcgacg gccgcatctt cactggtgtc aatgtatatc attttactgg
4621 gggaccttgt gcagaactcg tggtgctggg cactgctgct gctgcggcag ctggcaacct
4681 gacttgtatc gtcgcgatcg gaaatgagaa caggggcatc ttgagcccct gcggacggtg
4741 ccgacaggtg cttctcgatc tgcatcctgg gatcaaagcc atagtgaagg acagtgatgg
4801 acagccgacg gcagttggga ttcgtgaatt gctgccctct ggttatgtgt gggagggcta
4861 agcacaattc gagctcggta cctttaagac caatgactta caaggcagct gtagatctta
4921 gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag
4981 atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct
5041 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
5101 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
5161 cagtgtggaa aatctctagc agtagtagtt catgtcatct tattattcag tatttataac
5221 ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt tattgcagct tataatggtt
5281 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
5341 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggctctag ctatcccgcc
5401 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
5461 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca
5521 gaagtagtga ggaggctttt ttggaggcct agggacgtac ccaattcgcc ctatagtgag
5581 tcgtattacg cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc
5641 gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa
5701 gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atgggacgcg
5761 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
5821 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
5881 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
5941 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
6001 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
6061 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
6121 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
6181 aattttaaca aaatattaac gcttacaatt taggtggcac ttttcgggga aatgtgcgcg
6241 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
6301 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
6361 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
6421 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
6481 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
6541 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag
6601 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
6661 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
6721 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
6781 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
6841 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
6901 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
6961 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
7021 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
7081 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
7141 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
7201 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
7261 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
7321 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
7381 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
7441 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
7501 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
7561 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
7621 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
7681 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
7741 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
7801 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
7861 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
7921 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
7981 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
8041 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
8101 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga ag
//
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