| 质粒名称: | pMIH-TOMM20-Halo |
|---|---|
| 出品公司: | addgene |
| 目录编号: | 111626 |
| 存储实验室: | Benjamin Kile |
|
载体骨架基本信息 |
|
| 载体名称: | pMIH/MSCV-IRES-hygro |
| 载体出品公司: | Mark Van Delft |
| 空载体大小: | 6969 bp |
| 完整质粒大小: | 8340 bp |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | Hygromycin |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | NEB Stable |
| 拷贝数: | 未知 |
|
插入基因信息 |
|
| 插入基因名称: | TOMM20 |
| 物种来源: | M. musculus (mouse) |
| 插入大小: | 2782bp |
| 基因突变: | Tomm20 (a.k.a. 1810060K07Rik, BB284719, Gm19268, MAS20, MOM19, TOM20, mKIAA0016) |
| GenBank编号 | NM_014765.2 |
| 启动子: | MSCV |
| 标签/融合蛋白: | HaloTag (C terminal on insert) |
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | BglII(未破坏) |
| 3’克隆位点: | XhoI (未破坏) |
| 5’测序引物 | gaacctcctcgttcgaccc |
|
文献引用方法 |
|
| 材料和方法部分: | pMIH-TOMM20-Halo was a gift from Benjamin Kile (Addgene plasmid # 111626 ; http://n2t.net/addgene:111626 ; RRID:Addgene_111626) |
| 参考文献部分: | BAK/BAX macropores facilitate mitochondrial herniation and mtDNA efflux during apoptosis. McArthur K, Whitehead LW, Heddleston JM, Li L, Padman BS, Oorschot V, Geoghegan ND, Chappaz S, Davidson S, San Chin H, Lane RM, Dramicanin M, Saunders TL, Sugiana C, Lessene R, Osellame LD, Chew TL, Dewson G, Lazarou M, Ramm G, Lessene G, Ryan MT, Rogers KL, van Delft MF, Kile BT. Science. 2018 Feb 23;359(6378). pii: 359/6378/eaao6047. doi: 10.1126/science.aao6047. 10.1126/science.aao6047 PubMed 2947 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pMIH-TOMM20-Halo 8370 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Fusion protein used to visualise mitochondria upon the addition of
Halo specific dye, has hygromycin selection.
ACCESSION .
VERSION .
KEYWORDS pMIH-TOMM20-Halo
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8370)
AUTHORS McArthur K, Whitehead LW, Heddleston JM, Li L, Padman BS, Oorschot
V, Geoghegan ND, Chappaz S, Davidson S, San Chin H, Lane RM,
Dramicanin M, Saunders TL, Sugiana C, Lessene R, Osellame LD, Chew
TL, Dewson G, Lazarou M, Ramm G, Lessene G, Ryan MT, Rogers KL, van
Delft MF, Kile BT
TITLE BAK/BAX macropores facilitate mitochondrial herniation and mtDNA
efflux during apoptosis.
JOURNAL Science. 2018 Feb 23;359(6378). pii: 359/6378/eaao6047. doi:
10.1126/science.aao6047.
PUBMED 29472455
REFERENCE 2 (bases 1 to 8370)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..8370
/organism="synthetic DNA construct"
/mol_type="other DNA"
misc_feature 1..342
/label=MESV Psi
/note="packaging signal of murine embryonic stem cell
virus"
CDS 409..825
/label=gag (truncated)
/note="truncated Moloney murine leukemia virus (MMLV) gag
gene lacking the start codon"
primer_bind 755..777
/label=pLXSN 5'
/note="Murine stem cell virus, forward primer. Also called
MSCV"
primer_bind 793..809
/label=pBABE 5'
/note="Psi packaging signal, forward primer for pBABE
vectors"
regulatory 838..847
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 1312..2202
/codon_start=1
/product="modified bacterial dehalogenase that forms
covalent bonds with chloroalkane derivatives"
/label=HaloTag(R)
/translation="MAEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSS
YVWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVI
HDWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLII
DQNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALV
EEYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDL
IGSEIARWLSTLEISG"
misc_feature 2330..2792
/label=IRES
/note="internal ribosome entry site (IRES) of the
encephalomyocarditis virus (EMCV)"
primer_bind complement(2386..2403)
/label=IRES reverse
/note="IRES internal ribosome entry site, reverse primer.
Also called pCDH-rev"
primer_bind 2613..2632
/label=IRES-F
/note="IRES internal ribosome entry site, forward primer"
CDS 2831..3850
/codon_start=1
/gene="aph(4)-Ia"
/product="aminoglycoside phosphotransferase from E. coli"
/label=HygR
/note="confers resistance to hygromycin"
/translation="KPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYV
LRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPET
ELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTV
MDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGD
SQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNF
DDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE"
LTR 4185..4699
/label=3' LTR
/note="3' long terminal repeat from murine embryonic stem
cell virus"
protein_bind 4861..4877
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(4885..4915)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 4930..4951
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(5068..5085)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(5239..5827)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(5319..5338)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(5998..6858)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 6621..6640
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(6859..6963)
/gene="bla"
/label=AmpR promoter
primer_bind 7031..7049
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
primer_bind complement(7087..7109)
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind 7209..7228
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 7422..7444
/label=M13/pUC Forward
/note="In lacZ gene"
primer_bind 7572..7591
/label=pBRrevBam
/note="pBR322 vectors, tet region, downstream of BamHI,
reverse primer"
LTR 7791..8307
/label=5' LTR
/note="5' long terminal repeat from murine embryonic stem
cell virus"
ORIGIN
1 aagctggcca gcggtcgttt cgtgtctgtc tctgtctttg tgcgtgtttg tgccggcatc
61 taatgtttgc gcctgcgtct gtactagtta gctaactagc tctgtatctg gcggacccgt
121 ggtggaactg acgagttctg aacacccggc cgcaaccctg ggagacgtcc cagggacttt
181 gggggccgtt tttgtggccc gacctgagga agggagtcga tgtggaatcc gaccccgtca
241 ggatatgtgg ttctggtagg agacgagaac ctaaaacagt tcccgcctcc gtctgaattt
301 ttgctttcgg tttggaaccg aagccgcgcg tcttgtctgc tgcagcgctg cagcatcgtt
361 ctgtgttgtc tctgtctgac tgtgtttctg tatttgtctg aaaattaggg ccagactgtt
421 accactccct taagtttgac cttaggtcac tggaaagatg tcgagcggat cgctcacaac
481 cagtcggtag atgtcaagaa gagacgttgg gttaccttct gctctgcaga atggccaacc
541 tttaacgtcg gatggccgcg agacggcacc tttaaccgag acctcatcac ccaggttaag
601 atcaaggtct tttcacctgg cccgcatgga cacccagacc aggtccccta catcgtgacc
661 tgggaagcct tggcttttga cccccctccc tgggtcaagc cctttgtaca ccctaagcct
721 ccgcctcctc ttcctccatc cgccccgtct ctcccccttg aacctcctcg ttcgaccccg
781 cctcgatcct ccctttatcc agccctcact ccttctctag gcgccggaat tagatctgcc
841 accatggtgg gtcggaacag cgccatcgcc gccggtgtat gcggggccct tttcattggg
901 tactgcatct acttcgaccg caaaagacga agtgacccca acttcaagaa caggcttcga
961 gaacgaagaa agaaacagaa gcttgccaag gagagagctg ggctttccaa gttacctgac
1021 cttaaagatg ctgaagctgt tcagaagttc ttccttgaag aaatacagct tggtgaagag
1081 ttactagctc aaggtgaata tgagaagggc gtagaccatc tgacaaatgc aattgctgtg
1141 tgtggacagc cacagcagtt actgcaggtc ttacagcaaa ctcttccacc accagtgttc
1201 cagatgcttc tgactaagct cccaacaatt agtcagagaa ttgtaagtgc tcagagcttg
1261 gctgaagatg atgtggaagt cgacggtacc gcgggcccgg gatccaccgg tatggcagaa
1321 atcggtactg gctttccatt cgacccccat tatgtggaag tcctgggcga gcgcatgcac
1381 tacgtcgatg ttggtccgcg cgatggcacc cctgtgctgt tcctgcacgg taacccgacc
1441 tcctcctacg tgtggcgcaa catcatcccg catgttgcac cgacccatcg ctgcattgct
1501 ccagacctga tcggtatggg caaatccgac aaaccagacc tgggttattt cttcgacgac
1561 cacgtccgct tcatggatgc cttcatcgaa gccctgggtc tggaagaggt cgtcctggtc
1621 attcacgact ggggctccgc tctgggtttc cactgggcca agcgcaatcc agagcgcgtc
1681 aaaggtattg catttatgga gttcatccgc cctatcccga cctgggacga atggccagaa
1741 tttgcccgcg agaccttcca ggccttccgc accaccgacg tcggccgcaa gctgatcatc
1801 gatcagaacg tttttatcga gggtacgctg ccgatgggtg tcgtccgccc gctgactgaa
1861 gtcgagatgg accattaccg cgagccgttc ctgaatcctg ttgaccgcga gccactgtgg
1921 cgcttcccaa acgagctgcc aatcgccggt gagccagcga acatcgtcgc gctggtcgaa
1981 gaatacatgg actggctgca ccagtcccct gtcccgaagc tgctgttctg gggcacccca
2041 ggcgttctga tcccaccggc cgaagccgct cgcctggcca aaagcctgcc taactgcaag
2101 gctgtggaca tcggcccggg tctgaatctg ctgcaagaag acaacccgga cctgatcggc
2161 agcgagatcg cgcgctggct gtcgacgctt gaaatttccg gctgactcga ggttaacgaa
2221 ttccgccctc ccccccccct aacgttactg gccgaagccg cttggaataa ggccggtgtg
2281 cgtttgtcta tatgttattt tccaccatat tgccgtcttt tggcaatgtg agggcccgga
2341 aacctggccc tgtcttcttg acgagcattc ctaggggtct ttcccctctc gccaaaggaa
2401 tgcaaggtct gttgaatgtc gtgaaggaag cagttcctct ggaagcttct tgaagacaaa
2461 caacgtctgt agcgaccctt tgcaggcagc ggaacccccc acctggcgac aggtgcctct
2521 gcggccaaaa gccacgtgta taagatacac ctgcaaaggc ggcacaaccc cagtgccacg
2581 ttgtgagttg gatagttgtg gaaagagtca aatggctctc ctcaagcgta ttcaacaagg
2641 ggctgaagga tgcccagaag gtaccccatt gtatgggatc tgatctgggg cctcggtgca
2701 catgctttac atgtgtttag tcgaggttaa aaaaacgtct aggccccccg aaccacgggg
2761 acgtggtttt cctttgaaaa acacgatgat aagcttgcca caacccgtac caaagatgga
2821 tagatccgga aagcctgaac tcaccgcgac gtctgtcgag aagtttctga tcgaaaagtt
2881 cgacagcgtc tccgacctga tgcagctctc ggagggcgaa gaatctcgtg ctttcagctt
2941 cgatgtagga gggcgtggat atgtcctgcg ggtaaatagc tgcgccgatg gtttctacaa
3001 agatcgttat gtttatcggc actttgcatc ggccgcgctc ccgattccgg aagtgcttga
3061 cattggggaa ttcagcgaga gcctgaccta ttgcatctcc cgccgtgcac agggtgtcac
3121 gttgcaagac ctgcctgaaa ccgaactgcc cgctgttctg cagccggtcg cggaggccat
3181 ggatgcgatc gctgcggccg atcttagcca gacgagcggg ttcggcccat tcggaccgca
3241 aggaatcggt caatacacta catggcgtga tttcatatgc gcgattgctg atccccatgt
3301 gtatcactgg caaactgtga tggacgacac cgtcagtgcg tccgtcgcgc aggctctcga
3361 tgagctgatg ctttgggccg aggactgccc cgaagtccgg cacctcgtgc acgcggattt
3421 cggctccaac aatgtcctga cggacaatgg ccgcataaca gcggtcattg actggagcga
3481 ggcgatgttc ggggattccc aatacgaggt cgccaacatc ttcttctgga ggccgtggtt
3541 ggcttgtatg gagcagcaga cgcgctactt cgagcggagg catccggagc ttgcaggatc
3601 gccgcggctc cgggcgtata tgctccgcat tggtcttgac caactctatc agagcttggt
3661 tgacggcaat ttcgatgatg cagcttgggc gcagggtcga tgcgacgcaa tcgtccgatc
3721 cggagccggg actgtcgggc gtacacaaat cgcccgcaga agcgcggccg tctggaccga
3781 tggctgtgta gaagtactcg ccgatagtgg aaaccgacgc cccagcactc gtccgagggc
3841 aaaggaatag agtagatgcc gaccgaacaa gagctgattt cgagaacgcc tcagccagca
3901 actcgcgcga gcctagcaag gcaaatgcga gagaacggcc ttacgcttgg tggcacagtt
3961 ctcgtccaca gttcgctaag ctcgctcggc tgggtcgcgg gagggccggt cgcagtgatt
4021 caggcccttc tggattgtgt tggtccccag ggcacgattg tcatgcccac gcactcgggt
4081 gatctgactg atcccgcaga ttggagatcg ccgcccgtgc ctgccgattg ggtgcagatc
4141 cgataaaata aaagatttta tttagtctcc agaaaaaggg gggaatgaaa gaccccacct
4201 gtaggtttgg caagctagct taagtaacgc cattttgcaa ggcatggaaa atacataact
4261 gagaatagag aagttcagat caaggttagg aacagagaga cagcagaata tgggccaaac
4321 aggatatctg tggtaagcag ttcctgcccc ggctcagggc caagaacaga tggtccccag
4381 atgcggtccc gccctcagca gtttctagag aaccatcaga tgtttccagg gtgccccaag
4441 gacctgaaat gaccctgtgc cttatttgaa ctaaccaatc agttcgcttc tcgcttctgt
4501 tcgcgcgctt ctgctccccg agctcaataa aagagcccac aacccctcac tcggcgcgcc
4561 agtcctccga tagactgcgt cgcccgggta cccgtgtatc caataaaccc tcttgcagtt
4621 gcatccgact tgtggtctcg ctgttccttg ggagggtctc ctctgagtga ttgactaccc
4681 gtcagcgggg gtctttcatg ggtaacagtt tcttgaagtt ggagaacaac attctgaggg
4741 taggagtcga atattaagta atcctgactc aattagccac tgttttgaat ccacatactc
4801 caatactcct gaaatagttc attatggaca gcgcagaaag agctggggag aattgtgaaa
4861 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
4921 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
4981 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
5041 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
5101 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
5161 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
5221 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
5281 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
5341 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
5401 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
5461 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
5521 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
5581 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
5641 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
5701 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
5761 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
5821 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
5881 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
5941 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
6001 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
6061 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
6121 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
6181 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
6241 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
6301 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
6361 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
6421 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
6481 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
6541 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
6601 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
6661 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
6721 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
6781 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
6841 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
6901 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
6961 gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt
7021 aacctataaa aataggcgta tcacgaggcc ctttcgtctc gcgcgtttcg gtgatgacgg
7081 tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc
7141 cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct
7201 taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt gtgaaatacc
7261 gcacagatgc gtaaggagaa aataccgcat caggcgccat tcgccattca ggctgcgcaa
7321 ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg
7381 atgtgctgca aggcgattaa gttgggtaac gccagggttt tcccagtcac gacgttgtaa
7441 aacgacggcg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg
7501 gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga
7561 tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg
7621 atgccggcca cgatgcgtcc ggcgtagagg cgattagtcc aatttgttaa agacaggata
7681 tcagtggtcc aggctctagt tttgactcaa caatatcacc agctgaagcc tatagagtac
7741 gagccataga taaaataaaa gattttattt agtctccaga aaaagggggg aatgaaagac
7801 cccacctgta ggtttggcaa gctagcttaa gtaacgccat tttgcaaggc atggaaaata
7861 cataactgag aatagagaag ttcagatcaa ggttaggaac agagagacag cagaatatgg
7921 gccaaacagg atatctgtgg taagcagttc ctgccccggc tcagggccaa gaacagatgg
7981 tccccagatg cggtcccgcc ctcagcagtt tctagagaac catcagatgt ttccagggtg
8041 ccccaaggac ctgaaatgac cctgtgcctt atttgaacta accaatcagt tcgcttctcg
8101 cttctgttcg cgcgcttctg ctccccgagc tcaataaaag agcccacaac ccctcactcg
8161 gcgcgccagt cctccgatag actgcgtcgc ccgggtaccc gtattcccaa taaagcctct
8221 tgctgtttgc atccgaatcg tggactcgct gatccttggg agggtctcct cagattgatt
8281 gactgcccac ctcgggggtc tttcatttgg aggttccacc gagatttgga gacccctgcc
8341 cagggaccac cgaccccccc gccgggaggt
//
