| 质粒名称: | pMS2-GFP |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 27121 |
| 存储实验室: | Robert Singer |
|
载体骨架基本信息 |
|
| 载体名称: | pEGFP |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | -- |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | MS2 |
| 物种来源: | bacteriophage MS2 |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: |
HA（插入件上的N端子）
EGFP（插入件上的C端子）
SV40 NLS（插接件上的C端子）
|
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | NheI（销毁后未知） |
| 3' 克隆位点: | BamHI（销毁后未知） |
| 5' 测序引物 | M13_Rev |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pMS2-GFP was a gift from Robert Singer (Addgene plasmid # 27121 ; http://n2t.net/addgene:27121 ; RRID:Addgene_27121) |
| 参考文献部分: | Single mRNA molecules demonstrate probabilistic movement in living mammalian cells. Fusco D, Accornero N, Lavoie B, Shenoy SM, Blanchard JM, Singer RH, Bertrand E. Curr Biol. 2003 Jan 21. 13(2):161-7. 10.1016/S0960-9822(02)01436-7 PubMed 12546792 |
买家导航
pMS2GFP载体基本信息
pMS2GFP载体图谱质粒图谱和多克隆位点信息
pMS2GFP载体简介
pMS2GFP载体序列
LOCUS pMS2-GFP 6019 bp ds-DNA circular SYN 30-JUL-2019
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pMS2-GFP
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6019)
AUTHORS Fusco D, Accornero N, Lavoie B, Shenoy SM, Blanchard JM, Singer RH,
Bertrand E
TITLE Single mRNA molecules demonstrate probabilistic movement in living
mammalian cells.
JOURNAL Curr Biol. 2003 Jan 21. 13(2):161-7.
PUBMED 12546792
REFERENCE 2 (bases 1 to 6019)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Jul 30, 2019 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..6019
/organism="synthetic DNA construct"
/mol_type="other DNA"
CDS 750..776
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
CDS 777..788
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
regulatory 1173..1182
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 1179..1895
/codon_start=1
/product="enhanced GFP"
/label=EGFP
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
primer_bind complement(1224..1245)
/label=EGFP-N
/note="EGFP, reverse primer"
primer_bind complement(1485..1504)
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
primer_bind 1832..1853
/label=EGFP-C
/note="EGFP, forward primer"
CDS 1896..1916
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
intron 1975..2547
/label=-beta-globin intron
/note="intron from rabbit beta-globin gene"
primer_bind complement(2679..2698)
/label=Bglob-pA-R
/note="Rabbit beta-globin polyA region, reverse primer"
polyA_site 2744..2799
/label=-beta-globin poly(A) signal
/note="rabbit beta-globin polyadenylation signal"
primer_bind complement(2798..2817)
/label=rbglobpA-R
/note="Rabbit beta-globin polyA, reverse primer. Also
called rb-glob-pA-term-R"
primer_bind complement(3153..3172)
/label=T7
/note="T7 promoter, forward primer"
promoter complement(3154..3172)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(3179..3196)
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind complement(3179..3195)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(3188..3210)
/label=M13/pUC Forward
/note="In lacZ gene"
rep_origin 3337..3792
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(3424..3443)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 3634..3655
/label=F1ori-F
/note="F1 origin, forward primer"
promoter 3818..3922
/gene="bla"
/label=AmpR promoter
CDS 3923..4783
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(4141..4160)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 4954..5542
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 5443..5462
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 5696..5713
/label=L4440
/note="L4440 vector, forward primer"
protein_bind 5830..5851
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 5866..5896
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 5904..5920
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 5909..5931
/label=M13/pUC Reverse
/note="In lacZ gene"
primer_bind 5928..5944
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 5928..5944
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
promoter 5965..5983
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
1 tctagagtcg acctgcagcc caagcttggg ctgaacatcg agcgccaggg ctccgtaaag
61 ctactagagc acaggcggtg ccccaacgtc ctggggcctc tccactaata acggctactt
121 ccaattgatt ggacgcgcca tcttgcctgc cttatgcata ttcagcggtg aactgaatat
181 tcatgaacga ggcccgtccc gtccctccct ccttcccccc acccccggaa cccgctccgg
241 aggacccgaa gggccccgcc ttcattaccg atgcgtagga caaaccattt tcccgatgtg
301 tgtgggggga tactaatgag agactttagc tgaaaatgag cctgaactcc gaagctgagt
361 aaaaatggcc taactttatc ctccgttctg taagtcctcg gtttgagtgc acgggaaacc
421 cgaaaggagg acgacaggac caggaacatt ctcctcctcc tgtcgcgtca gaaagaacac
481 ccaaccaggg agccggagcc ctagcgtcaa caactccgcc gcgcgcgctc cgtgtaggcc
541 ggtgcgggcg gccccgtagc gcaagggagg gcgggaaagg aaggggcggg acacaagggc
601 gaatctataa agggcgtcac tcagccagtt ctctcctcag aagcgccgag agcgcgaccg
661 ggacggttgg agaagaaggt ggctcccgga agggggagag acaaactgcc gtaacctctg
721 ccgttcagga tcactagtcc accatgggct acccctacga cgtgcccgac tacgccatcg
781 aaggccgcca tatgctagcc gttaaaatgg cttctaactt tactcagttc gttctcgtcg
841 acaatggcgg aactggcgac gtgactgtcg ccccaagcaa cttcgctaac gggatcgctg
901 aatggatcag ctctaactcg cgttcacagg cttacaaagt aacctgtagc gttcgtcaga
961 gctctgcgca gaatcgcaaa tacaccatca aagtcgaggt gcctaaaggc gcctggcgtt
1021 cgtacttaaa tatggaacta accattccaa ttttcgccac gaattccgac tgcgagctta
1081 ttgttaaggc aatgcaaggt ctcctaaaag atggaaaccc gattccctca gcaatcgcag
1141 caaactccgg catctacgcg gatccaccgg tcgccaccat ggtgagcaag ggcgaggagc
1201 tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt
1261 tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca
1321 tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgacctacg
1381 gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg
1441 ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca
1501 agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg
1561 gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca
1621 gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg aacttcaaga
1681 tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc
1741 ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagtccgccc
1801 tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg
1861 ccgggatcac tctcggcatg gacgagctgt acaagccaaa aaagaagaga aaggtataaa
1921 tcgattctgc agtagctcgc cggcagctga ctagctagag ctgagaactt cagggtgagt
1981 ttggggaccc ttgattgttc tttctttttc gctattgtaa aattcatgtt atatggaggg
2041 ggcaaagttt tcagggtgtt gtttagaatg ggaagatgtc ccttgtatca ccatggaccc
2101 tcatgataat tttgtttctt tcactttcta ctctgttgac aaccattgtc tcctcttatt
2161 ttcttttcat tttctgtaac tttttcgtta aactttagct tgcatttgta acgaattttt
2221 aaattcactt ttgtttattt gtcagattgt aagtactttc tctaatcact tttttttcaa
2281 ggcaatcagg gtatattata ttgtacttca gcacagtttt agagaacaat tgttataatt
2341 aaatgataag gtagaatatt tctgcatata aattctggct ggcgtggaaa tattcttatt
2401 ggtagaaaca actacaccct ggtcatcatc ctgcctttct ctttatggtt acaatgatat
2461 acactgtttg agatgaggat aaaatactct gagtccaaac cgggcccctc tgctaaccat
2521 gttcatgcct tcttctcttt cctacagctc ctgggcaacg tgctggttgt tgtgctgtct
2581 catcattttg gcaaagaatt cactcctcag gtgcaggctg cctatcagaa ggtggtggct
2641 ggtgtggcca atgccctggc tcacaaatac cactgagatc tttttccctc tgccaaaaat
2701 tatggggaca tcatgaagcc ccttgagcat ctgacttctg gctaataaag gaaatttatt
2761 ttcattgcaa tagtgtgttg gaattttttg tgtctctcac tcggaaggac atatgggagg
2821 gcaaatcatt taaaacatca gaatgagtat ttggtttaga gtttggcaac atatgcccat
2881 atgctggctg ccatgaacaa aggttggcta taaagaggtc atcagtatat gaaacagccc
2941 cctgctgtcc attccttatt ccatagaaaa gccttgactt gaggttagat tttttttata
3001 ttttgttttg tgttattttt ttctttaaca tccctaaaat tttccttaca tgttttacta
3061 gccagatttt tcctcctctc ctgactactc ccagtcatag ctgtccctct tctcttatgg
3121 agatccctcg agcggccgcg gtacccaatt cgccctatag tgagtcgtat tacaattcac
3181 tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc
3241 ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc
3301 cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa
3361 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
3421 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
3481 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
3541 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
3601 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
3661 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct
3721 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa
3781 cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
3841 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
3901 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
3961 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
4021 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
4081 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct
4141 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat
4201 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
4261 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
4321 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
4381 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
4441 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac
4501 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
4561 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
4621 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc
4681 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
4741 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta
4801 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
4861 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
4921 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
4981 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
5041 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
5101 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
5161 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
5221 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
5281 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
5341 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
5401 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
5461 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
5521 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
5581 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
5641 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga
5701 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
5761 gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg
5821 caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct
5881 tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta
5941 tgaccatgat tacgccaagc tcgaaattaa ccctcactaa agggaacaaa agctggagct
6001 ccaccgcggt ggcggccgc
//
