| 质粒名称: | pMS2M-PH-gagpol-D64V |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 166031 |
| 存储实验室: | Yujia Cai |
|
载体骨架基本信息 |
|
| 载体名称: | pGFP-PH-gagpol-D64V |
| 载体出品公司: | -- |
| 空载体大小: | -- |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 慢病毒载体 |
| 筛选标记: | -- |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | NEB Stable |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | MS2-Gag-Pol |
| 物种来源: | HIV-1, Bacteriophage |
| 插入大小: | -- |
| 基因突变: | D64V mutation within the integrase within Pol |
| GenBank编号 | -- |
| 启动子: | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | -- |
| 3’克隆位点: | -- |
| 5’测序引物 | -- |
| 3’测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pMS2M-PH-gagpol-D64V was a gift from Yujia Cai (Addgene plasmid # 166031 ; http://n2t.net/addgene:166031 ; RRID:Addgene_166031) |
| 参考文献部分: | Lentiviral delivery of co-packaged Cas9 mRNA and a Vegfa-targeting guide RNA prevents wet age-related macular degeneration in mice. Ling S, Yang S, Hu X, Yin D, Dai Y, Qian X, Wang D, Pan X, Hong J, Sun X, Yang H, Paludan SR, Cai Y. Nat Biomed Eng. 2021 Jan 4. pii: 10.1038/s41551-020-00656-y. doi: 10.1038/s41551-020-00656-y. 10.1038/s41551-020-00656-y PubMed 33398131 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pMS2M-PH-gagpol-D64 10711 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Expression of bacteriophage-derived MS2 coat protein fused HIV-1
Lentiviral Gag-Pol at N terminal with a HIV-1 protease cleavage
signal between MS2 and Gag-Pol. .
ACCESSION .
VERSION .
KEYWORDS pMS2M-PH-gagpol-D64V
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 10711)
AUTHORS Ling S, Yang S, Hu X, Yin D, Dai Y, Qian X, Wang D, Pan X, Hong J,
Sun X, Yang H, Paludan SR, Cai Y
TITLE Lentiviral delivery of co-packaged Cas9 mRNA and a Vegfa-targeting
guide RNA prevents wet age-related macular degeneration in mice.
JOURNAL Nat Biomed Eng. 2021 Jan 4. pii: 10.1038/s41551-020-00656-y. doi:
10.1038/s41551-020-00656-y.
PUBMED 33398131
REFERENCE 2 (bases 1 to 10711)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..10711
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind complement(44..63)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
enhancer 235..614
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 615..818
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 769..789
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
regulatory 857..866
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 866..1252
/codon_start=1
/product="bacteriophage MS2 coat protein"
/label=MS2-N55K
/note="binds with high affinity to a specific stem-loop
structure in the viral RNA (Lim et al., 1994)"
/translation="ASNFTQFVLVDNGGTGDVTVAPSNFANGVAEWISSNSRSQAYKVT
CSVRQSSAQKRKYTIKVEVPKVATQTVGGVELPVAAWRSYLNMELTIPIFATNSDCELI
VKAMQGLLKDGNPIPSAIAANSGIY"
misc_feature 1277..1342
/label=MCS
/note="multiple cloning site"
promoter complement(6265..6283)
/label=SP6 promoter
/note="promoter for bacteriophage SP6 RNA polymerase"
primer_bind complement(6266..6283)
/label=SP6
/note="SP6 promoter, forward primer"
primer_bind complement(6303..6320)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 6309..6533
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 6579..7006
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(6666..6685)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 6875..6896
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind complement(7015..7035)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 7020..7349
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 7200..7335
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 7262..7281
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
CDS 7416..8210
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(7470..7489)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 8080..8099
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
polyA_signal 8384..8505
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(8421..8440)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 8475..8494
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind complement(8554..8570)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(8554..8570)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(8567..8589)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 8578..8594
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(8602..8632)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 8647..8668
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(8785..8802)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(8956..9544)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(9036..9055)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(9715..10575)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 10338..10357
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(10576..10680)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
781 gtaggcgtgt acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc
841 gctagcgcta ccggtcgcca ccatggcctc taattttact caatttgtgc ttgtcgataa
901 tggggggacg ggagatgtga ccgttgcccc tagcaatttc gcaaatggcg ttgcagaatg
961 gatctctagc aacagcagaa gccaagcgta caaagtaacg tgttccgttc gccaaagctc
1021 cgcccaaaaa cggaagtata caataaaggt tgaggtgccg aaagtagcca ctcaaacagt
1081 tggtggggta gaattgcccg tagcggcatg gcggtcatat ctcaatatgg aactcactat
1141 cccaatcttc gccacgaata gcgattgtga gctgatagtt aaggctatgc aaggtcttct
1201 caaagatgga aaccctattc catctgctat cgccgccaac agcgggatat acagccagaa
1261 ctatccgatt gtgcagtccg gactcagatc tcgagctcaa gcttcgaatt ctgcagtcga
1321 cggtaccgcg ggcccgggat ccatggactc gggccgggac ttcctgaccc tgcacggcct
1381 acaggatgat gaggatctac aggcgctgct gaagggcagc cagctcctga aggtgaagtc
1441 cagctcatgg aggagagagc gcttctacaa gttgcaggag gactgcaaga ccatctggca
1501 ggagtcccgc aaggtcatgc ggaccccgga gtcccagctg ttctccatcg aggacattca
1561 ggaggtgcga atggggcacc gcacggaggg tctggagaag ttcgcccgtg atgtgcccga
1621 ggaccgctgc ttctccattg tcttcaagga ccagcgcaat acactagacc tcatcgcccc
1681 atcgccagct gatgcccagc actgggtgct ggggctgcac aagatcatcc accactcagg
1741 ctccatggac cagcgtcaga agctacagca ctggattcac tcctgcttgc gaaaagctga
1801 caaaaacaag gacaacaaga tgagcttcaa ggagctgcag aacttcctga aggagctcaa
1861 catccagcct cgagccgaat tcaccctggc cgccagggcc agcgtgctga gcggcggcga
1921 gctggacagg tgggagaaga tcaggctgag gcccggcggc aagaagaagt ataagctgaa
1981 gcacatcgtg tgggccagca gggagctgga gaggttcgcc gtgaaccccg gcctgctgga
2041 gaccagcgag ggctgcaggc agatcctggg ccagctgcag cccagcctgc agaccggcag
2101 cgaggagctg aggagcctgt acaacaccgt ggccaccctg tactgcgtgc accagaggat
2161 cgagatcaag gacaccaagg aggccctgga caagatcgag gaggagcaga acaagtccaa
2221 gaagaaggcc cagcaggccg ccgccgacac cggccacagc agccaggtga gccagaacta
2281 ccccatcgtg cagaacatcc agggccagat ggtgcaccag gccatcagcc ccaggaccct
2341 gaacgcctgg gtgaaggtgg tggaggagaa ggccttcagc cccgaggtga tccccatgtt
2401 cagcgccctg agcgagggag ccacccccca ggacctgaac accatgctga acaccgtggg
2461 cggccaccag gccgccatgc agatgctgaa ggagaccatc aacgaggagg ccgccgagtg
2521 ggacagggtg caccccgtgc acgccggccc catcgccccc ggccagatga gggagccccg
2581 cggcagcgac atcgccggca ccaccagcac cctgcaggag cagatcggct ggatgaccaa
2641 caaccccccc atccccgtgg gcgaaatcta caagaggtgg atcatcctgg gcctgaacaa
2701 gatcgtgagg atgtacagcc ccaccagcat cctggatatc aggcagggcc ccaaagagcc
2761 cttcagggac tacgtggaca ggttctacaa gaccctgcgc gccgagcagg ccagccagga
2821 ggtgaagaac tggatgaccg agaccctgct ggtgcagaac gccaaccccg actgcaagac
2881 catcctgaag gccctgggac ccgccgccac cctggaggag atgatgaccg cctgccaggg
2941 cgtgggcggc cccggccaca aggccagggt gctggccgag gccatgagcc aggtgaccaa
3001 caccgccacc atcatgatgc agaggggcaa cttcaggaac cagaggaaga tggtgaagtg
3061 cttcaactgc ggcaaggagg gccacaccgc caggaactgc cgcgccccca ggaagaaggg
3121 ctgctggaag tgcggcaagg agggccacca gatgaaggac tgcaccgaga ggcaggctaa
3181 ttttttaggg aagatctggc cttcctacaa gggaaggcca gggaattttc ttcagagcag
3241 accagagcca acagccccac catttcttca gagcagacca gagccaacag ccccaccaga
3301 agagagcttc aggtctgggg tagagacaac aactccccct cagaagcagg agccgataga
3361 caaggaactg tatcctttaa cttccctcag atcactcttt ggcaacgacc cctcgtcaca
3421 ataaagatcg gtggccagct gaaggaggcc ctgctggaca ccggcgccga cgacaccgtg
3481 ctggaggaga tgagcctgcc cggcaggtgg aagcccaaga tgatcggcgg catcggcggc
3541 ttcatcaagg tgaggcagta cgaccagatc ctgatcgaga tctgcggcca caaggccatc
3601 ggcaccgtgc tggtgggacc tacacctgtg aacatcatcg gcaggaacct gctgacccag
3661 atcggctgca ccctgaactt ccccatcagc cccatcgaga ccgtgcccgt gaagctgaag
3721 cccggcatgg acggccctaa ggtgaagcag tggcccctga ccgaggagaa gatcaaggcc
3781 ctggtggaga tctgcaccga gatggagaag gagggcaaga tcagcaagat cggccccgag
3841 aacccctaca acacccccgt gttcgccatc aagaagaagg acagcaccaa gtggaggaag
3901 ctggtggact tcagggagct gaacaagagg acccaggact tctgggaggt gcagctgggc
3961 atcccccacc ccgccggcct gaagaagaag aagagcgtga ccgtgctgga cgtgggcgac
4021 gcctacttca gcgtgcccct ggacgaggac ttcaggaagt ataccgcctt caccatcccc
4081 agcatcaaca acgagacccc cggcatccgc taccagtaca acgtgctgcc ccagggctgg
4141 aagggcagcc ccgccatctt ccagagcagc atgacaaaga tcctggagcc cttcaagaag
4201 cagaaccccg acatcgtgat ctatcagtac atggacgacc tgtacgtggg cagcgacctg
4261 gagatcggcc agcacaggac caagatcgag gagctgaggc agcacctgct gaggtggggc
4321 ctgaccaccc ccgacaagaa gcaccagaag gagcccccat tcctgtggat gggctacgag
4381 ctgcaccccg acaagtggac cgtgcagccc atcgtgctgc ccgagaagga cagctggacc
4441 gtgaacgaca ttcagaagct ggtgggcaag ctgaactggg ccagccagat ctaccctggc
4501 atcaaggtga ggcagctgtg caagctgctg aggggcacaa aggctctgac cgaggtgatc
4561 cccctgaccg aggaggccga gctggagctg gccgagaaca gggagatcct gaaggagccc
4621 gtgcacggcg tgtactacga ccccagcaag gacctgatcg ccgagatcca gaagcagggc
4681 cagggccagt ggacctacca gatctaccag gagcccttca agaacctgaa gaccggcaag
4741 tacgcccgca tgcgcggcgc ccacaccaac gacgtgaagc agctgaccga ggccgtgcag
4801 aagatcacca ccgagagcat cgtgatctgg ggcaagactc ctaagttcaa gctgcccatc
4861 cagaaggaga cctgggagac ctggtggacc gagtactggc aggccacctg gattcccgag
4921 tgggagttcg tgaacacccc tcccctggtg aagctgtggt atcagctgga gaaggagccc
4981 atcgtgggcg ccgagacctt ctacgtggac ggcgccgcca acagggagac caagctgggc
5041 aaggccggct acgtgaccaa caagggccgc cagaaggtgg tgcccctgac caacaccacc
5101 aaccagaaga ccgagctgca ggctatctac ctggccctgc aggactcagg cctggaggtg
5161 aacatcgtga ccgacagcca gtacgccctg ggcatcatcc aggcccagcc cgacaagagc
5221 gagagcgagc tggtgaacca gatcatcgag cagctgatca agaaggagaa ggtgtacctg
5281 gcctgggtgc ccgcccacaa gggcatcggc ggcaacgagc aggtggacaa gctggtgagc
5341 gccggcatca ggaagatcct gttcctggac ggcatcgaca aggcccagga cgagcacgag
5401 aagtaccaca gcaactggag ggctatggct agcgacttca acctgcctcc cgtggtggct
5461 aaggagatcg tggccagctg cgacaagtgc cagctgaagg gcgaggccat gcacggccag
5521 gtggactgca gccccggcat ctggcagctg gtttgcaccc acctggaggg caaggtgatc
5581 ctggtggccg tgcacgtggc ctccggctac atcgaggccg aggtgatccc cgccgagacc
5641 ggccaggaga ccgcctactt cctgctgaag ctggccggcc gctggcccgt gaagaccatc
5701 cacaccgaca acggcagcaa cttcaccagc gccaccgtga aggctgcctg ctggtgggcc
5761 ggcatcaagc aggagttcgg catcccctac aacccccagt ctcagggcgt ggtggagagc
5821 atgaacaagg agctgaagaa gatcatcggc caggtgaggg accaggccga gcacctgaag
5881 accgccgtgc agatggccgt gttcatccac aacttcaaga ggaagggcgg catcggcggc
5941 tacagcgccg gcgagaggat cgtggacatc atcgccaccg acatccagac caaggagctg
6001 cagaagcaga tcaccaagat ccagaacttc agggtgtact acagggacag caggaaccct
6061 ctgtggaagg gccccgccaa gctgctgtgg aagggcgagg gcgccgtggt gatccaggac
6121 aacagcgaca tcaaggtggt gcccaggagg aaggccaaga tcatcaggga ctacggcaag
6181 cagatggccg gcgacgactg cgtggcctcc aggcaggacg aggactgata aggatccctc
6241 gagcatgcat ctagagggcc ctattctata gtgtcaccta aatgctagag ctcgctgatc
6301 agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc
6361 cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc
6421 gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg
6481 ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga
6541 ggcggaaaga accagctggg gctctagggg gtatccccac gcgccctgta gcggcgcatt
6601 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc
6661 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
6721 agctctaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
6781 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
6841 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
6901 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
6961 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt ctgtggaatg
7021 tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
7081 tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa
7141 gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta actccgccca
7201 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
7261 ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag tagtgaggag
7321 gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata tccattttcg
7381 gatctgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg
7441 caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa
7501 tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg
7561 tcaagaccga cctgtccggt gccctgaatg aactgcagga cgaggcagcg cggctatcgt
7621 ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa
7681 gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc
7741 ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg
7801 ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg
7861 aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg
7921 aactgttcgc caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg
7981 gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact
8041 gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg
8101 ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc
8161 ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga gcgggactct
8221 ggggttcgaa atgaccgacc aagcgacgcc caacctgcca tcacgagatt tcgattccac
8281 cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat
8341 cctccagcgc ggggatctca tgctggagtt cttcgcccac cccaacttgt ttattgcagc
8401 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
8461 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctgtatacc
8521 gtcgacctct agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg
8581 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg
8641 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
8701 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
8761 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
8821 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
8881 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
8941 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
9001 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
9061 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
9121 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
9181 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
9241 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
9301 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
9361 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
9421 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
9481 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
9541 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
9601 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
9661 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
9721 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
9781 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
9841 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
9901 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
9961 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
10021 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
10081 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
10141 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
10201 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
10261 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
10321 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
10381 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
10441 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
10501 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
10561 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
10621 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
10681 cacatttccc cgaaaagtgc cacctgacgt c
//
