| 质粒名称: | pMXs-c-Myc |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 13375 |
| 存储实验室: | Shinya Yamanaka |
|
载体骨架基本信息 |
|
| 载体名称: | pMXs |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 4600 bp |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳动物表达，逆转录病毒 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | myelocytomatosis oncogene,c-Myc |
| 物种来源: | M. musculus (mouse) |
| 插入大小: | 1320 bp |
| 基因突变: | None |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | pMX-S1811 |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pMXs-c-Myc was a gift from Shinya Yamanaka (Addgene plasmid # 13375 ; http://n2t.net/addgene:13375 ; RRID:Addgene_13375) |
| 参考文献部分: | Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. Takahashi K, Yamanaka S. Cell. 2006 Aug 25. 126(4):663-76. 10.1016/j.cell.2006.07.024 PubMed 16904174 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pMXs-c-Myc 6087 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION Retroviral expression of mouse c-Myc.
ACCESSION .
VERSION .
KEYWORDS pMXs-c-Myc
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6087)
AUTHORS Takahashi K, Yamanaka S
TITLE Induction of pluripotent stem cells from mouse embryonic and adult
fibroblast cultures by defined factors.
JOURNAL Cell. 2006 Aug 25. 126(4):663-76.
PUBMED 16904174
REFERENCE 2 (bases 1 to 6087)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..6087
/organism="synthetic DNA construct"
/mol_type="other DNA"
LTR 120..589
/note="long terminal repeat from Moloney murine leukemia
virus"
protein_bind 906..930
/gene="mutant version of attB"
/label=attB2
/bound_moiety="BP Clonase(TM)"
/note="recombination site for the Gateway(R) BP reaction"
protein_bind complement(2287..2311)
/gene="mutant version of attB"
/label=attB1
/bound_moiety="BP Clonase(TM)"
/note="recombination site for the Gateway(R) BP reaction"
misc_feature 2362..2736
/label=pol region
/note="Moloney murine leukemia virus (MMLV) pol region
containing the splice acceptor site"
primer_bind complement(2408..2424)
/label=pBMN 5'
/note="MMLV sequence, for inserts in pBMN retroviral
vector"
CDS complement(2746..3162)
/label=gag (truncated)
/note="truncated Moloney murine leukemia virus (MMLV) gag
gene lacking the start codon"
primer_bind complement(2762..2778)
/label=pBABE 5'
/note="Psi packaging signal, forward primer for pBABE
vectors"
primer_bind complement(2794..2816)
/label=pLXSN 5'
/note="Murine stem cell virus, forward primer. Also called
MSCV"
misc_feature 3221..3578
/label=MMLV Psi
/note="packaging signal of Moloney murine leukemia virus
(MMLV)"
promoter 4189..4293
/gene="bla"
/label=AmpR promoter
CDS 4294..5154
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(4512..4531)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 5318..5906
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 5807..5826
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 6060..6077
/label=L4440
/note="L4440 vector, forward primer"
ORIGIN
1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcaactatg
61 gccatttaat gtaaatactt aagaaaaaaa accaaattaa ttttgataca tgctgcatgt
121 gaaagacccc cgctgacggg tagtcaatca ctcagaggag accctcccaa ggaacagcga
181 gaccacaagt cggatgcaac tgcaagaggg tttattggat acacgggtac ccgggcgact
241 cagtcaatcg gaggactggc gcgccgagtg aggggttgtg ggctctttta ttgagctcgg
301 ggagcagaag cgcgcgaaca gaagcgagaa gcgaactgat tggttagttc aaataaggca
361 cagggtcatt tcaggtcctt ggggcaccct ggaaacatct gatggttctc tagaaactgc
421 tgagggctgg accgcatctg gggaccatct gttcttggcc ctgagccggg gcaggaactg
481 cttaccacag atatcctgtt tggcccatat tcagctgttc catctgttct tggccctgag
541 ccggggcagg aactgcttac cacagatatc ctgtttggcc catattcagc tgttccatct
601 gttcctgacc ttgatctgaa cttctctatt ctcagttatg tatttttcca tgccttgcaa
661 aatggcgtta cttaagctag cttgccaaac ctacaggtgg ggtctttcat tccccccttt
721 ttctggagac taaataaaat cttttatttt atcgtcgacc actgtgctgg cggccgctcg
781 agtttaaata gtacataatg gatttcctta cgcgaaatac gggcagacat ggcctgcccg
841 gttattatta tttttgacac cagaccaact ggtaatggta gcgaccggcg ctcagctgga
901 atatcaccac tttgtacaag aaagctgggt cggcgcgccc acccttttat gcaccagagt
961 ttcgaagctg ttcgagtttg tgtttcaact gttctcgtcg tttcctcaat aagtcctttt
1021 cagaggtgag cttgtgctcg tctgcttgaa tggacaggat gtaggcggtg gcttttttga
1081 ggatcactac cttgggggcc ttttcgttgt tttccaattc agggatctgg tcacgcaggg
1141 caaaaaagct gcgcttcagc tcgttcctcc tctgacgttc caagacgttg tgtgtccgcc
1201 tcttgtcgtt ttcctccgtg tctgaggacc tggggctgga gcacttgcgg ttgttgctga
1261 tctgcttcag gaccctgcca ctgtccaact tggccctctt ggcagctgga tagtccttcc
1321 ttgtggaggg gggtgcggcg tagttgtgct ggtgagtgga gacgtggcac ctcttgagga
1381 ccagtgggct gtgcggaggt ttgctgtggc ctcgggatgg agatgagccc gactccgacc
1441 tcttggcagg ggtttgcctc ttctccacag acaccacatc aatttcttcc tcatcttctt
1501 gctcttcttc agagtcgctg ctggtggtgg gcggtgtctc ctcatgcagc actaggggct
1561 cagggctggc ccgtggggag gactcggagg acagcagcga gtccgaggaa ggagagaagg
1621 ccgtggaatc ggacgaggta caggatttgg gcgagctgct gtcgttgagc gggtagggaa
1681 agaccactga ggggtcaatg cactcggacg cggcggcggt gaggtcctgc aggtacaggc
1741 tggaggtgga gcagacgctg tgcccgcggg cggggctcag gctggtgctg tctttgcgcg
1801 cagcctggta ggaggccagc ttctccgaga ccagcttggc agcggctgag aaaccgctcc
1861 acatacagtc ctggatgatg atgttcttga tgaaggtctc gtcgtcagga tcgcagatga
1921 agctctggtt caccatgtct cctccaagta actcggtcat catctccagc tgatcggcgg
1981 tggagaagtt gccaccgccg ccgtcatcgt cttcccttgg ggagaaggac gtagcgaccg
2041 caacatagga tggagagcag agcccggagc ggcggctcgg ggacaggggc ggggtgggaa
2101 gcagctcgaa tttcttccag atatcctcac tgggcgcggg cggctgcagc tcgctctgct
2161 gttgctggtg atagaaattc tcttcctcgt cgcagatgaa atagggctgt acggagtcgt
2221 agtcgaggtc atagttcctg ttggtgaagt tcacgttgag gggcatggtg aagggggcgg
2281 ccgcggagcc tgcttttttg tacaaacttg tgatttcccg taccaccaca ctgggatcct
2341 taattaacta gctagatccg gcagtctaga ggatggtcca cccccggggt cggcagcctt
2401 cacgtgggcg gcgtgtatcc aagctgcgat gccgtctact ttgagggcgg tgggggtggt
2461 cagcaggact gtgtaaggtc ctttccagcg aggttctagg ttcttagtct ggtgtcggcg
2521 gacccacact gtgtcgccga ctcggtaagg gtgaggtacc accggtcggt ccagttgttc
2581 ttggtaggct gccgccagag gtctccagac ttcgtgctgg actaagtaga gagcctgtaa
2641 gtgagcttgg agagaggggc tgttagtaac tcttgtcatg tcagggtcag ggaagtttac
2701 aaggggcggg ggtgccccat ataagatctc atatggccat atgggggcgc ctagagaagg
2761 agtgagggct ggataaaggg aggatcgagg cggggtcgaa cgaggaggtt caagggggag
2821 agacggggcg gatggaggaa gaggaggcgg aggcttaggg tgtacaaagg gcttgaccca
2881 gggagggggg tcaaaagcca aggcttccca ggtcacgatg taggggacct ggtctgggtg
2941 tccatgcggg ccaggtgaaa agaccttgat cttaacctgg gtgatgaggt ctcggttaaa
3001 ggtgccgtct cgcggccatc cgacgttaaa ggttggccat tctgcagagc agaaggtaac
3061 ccaacgtctc ttcttgacat ctaccgactg gttgtgagcg atccgctcga catctttcca
3121 gtgacctaag gtcaaactta agggagtggt aacagtctgg ccctaatttt cagacaaata
3181 cagaaacaca gtcagacaga gacaacacag aacgatgctg cagcagacaa gacgcgcggc
3241 gcggcttcgg tcccaaaccg aaagcaaaaa ttcagacgga ggcgggaact gttttaggtt
3301 ctcgtctcct accagaacca catatccctc ctattagggg ggtgcaccaa agagtccaaa
3361 acgatcggga tttttggact caggtcgggc cacaaaaacg gcccccgaag tccctgggac
3421 gtctcccagg gttgcggccg ggtgttccga actcgtcagt tccaccacgg gtccgccaga
3481 tacagagcta gttagctaac tagtaccgac gcaggcgcat aaaatcagtc atagacacta
3541 gacaatcgga cagacacaga taagttgctg gccagcttac ctcccggtgg tgggtcggtg
3601 gtccctgggc aggggtctcc cgatcccgga cgagccccca aatgaaagac ccccgctgac
3661 gggtagtcaa tcactcagag gagaccctcc caaggaacag cgagaccaca agtcggatgc
3721 aactgcaaga gggtttattg gatacacggg tacccgggcg actcagtcaa tcggaggact
3781 ggcgccccga gtgaggggtt gtgggctctt ttattgagct cggggagcag aagcgcgcga
3841 acagaagcga gaagcgaact gattggttag ttcaaataag gcacagggtc atttcaggtc
3901 cttggggcac cctggaaaca tctgatggtt ctctagaaac tgctgagggc tggaccgcat
3961 ctggggacca tctgttcttg gccctgagcc ggggcaggaa ctgcttacca cagatatcct
4021 gtttggccca tattcagctg ttccatctgt tcctgacctt gatctgaact tttctattct
4081 cagttatgta tttttccatg ccttgcaaaa tggcgttact taagctagct tgccaaacct
4141 acaggtgggg tctttcatta tgcaggtggc acttttcggg gaaatgtgcg cggaacccct
4201 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
4261 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
4321 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
4381 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
4441 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
4501 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc
4561 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
4621 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
4681 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
4741 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
4801 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
4861 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
4921 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
4981 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
5041 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
5101 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
5161 gaccaagttt actcatatat actttagatt gatttgcggc cggccgcaaa aggatctagg
5221 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
5281 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
5341 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
5401 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
5461 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
5521 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
5581 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
5641 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
5701 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
5761 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
5821 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
5881 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
5941 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
6001 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
6061 gcgagtcagt gagcgaggaa gcggaag
//
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