| 质粒名称: | pMXs-hSOX2 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 17218 |
| 存储实验室: | Shinya Yamanaka |
|
质粒骨架基本信息 |
|
| 质粒名称: | pMXs |
| 质粒抗性: | 氨苄霉素 |
| 空质粒大小: | 4600 bp |
| 质粒类型: | 哺乳细胞表达 |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | SOX2 |
| 物种来源: | H. sapiens (human) |
| 插入大小: | 960 bp |
| 基因突变: | None |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | attB1(未破坏) |
| 3' 克隆位点: | attB2(未破坏) |
| 5' 测序引物 | pMX-S1811 |
| 3' 测序引物 | |
|
文献引用方法 |
|
| 材料和方法部分: | pMXs-hSOX2 was a gift from Shinya Yamanaka (Addgene plasmid # 17218) |
| 参考文献部分: | Induction of pluripotent stem cells from adult human fibroblasts by defined factors. Takahashi K, Tanabe K, Ohnuki M, Narita M, Ichisaka T, Tomoda K, Yamanaka S. Cell. 2007 Nov 30. 131(5):861-72. 10.1016/j.cell.2007.11.019 PubMed 18035408 |
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载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pMXs-hSOX2 Sequencing Result 5721 bp DNA SYN
DEFINITION pMXs-hSOX2 Sequencing Result
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
FEATURES Location/Qualifiers
source 1..5721
/organism="pMXs-hSOX2 Sequencing Result"
/mol_type="other DNA"
misc_feature complement(130..636)
/label="3MoMuLV_LTR"
misc_feature complement(130..594)
/label="5_LTR2"
misc_feature complement(831..855)
/label="attB2"
CDS complement(872..1825)
/label="ORF frame 3"
misc_feature complement(1846..1870)
/label="attB1"
misc_feature complement(1919..3200)
/label="psi_plus_pack2"
misc_feature complement(1967..1983)
/label="pBMN_5_primer"
misc_feature complement(2236..2715)
/label="gag"
misc_feature complement(2321..2337)
/label="pBABE_5_primer"
misc_feature complement(2353..2375)
/label="pLXSN_5_primer"
misc_feature complement(2353..2375)
/label="MSCV_primer"
misc_feature complement(3200..3751)
/label="5_LTR2"
misc_feature complement(3200..3793)
/label="3MoMuLV_LTR"
promoter 3858..3886
/label="AmpR_promoter"
gene 3928..4788
/label="Ampicillin"
/gene="Ampicillin"
CDS 3928..4788
/label="ORF frame 1"
rep_origin 4936..5555
/label="pBR322_origin"
ORIGIN
1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgCAACTATG
61 GCCATTTAAT GTAAATACTT AAGAAAAAAA ACCAAATTAA TTTTGATACA TGCTGCATGT
121 GAAAGACCCC CGCTGACGGG TAGTCAATCA CTCAGAGGAG ACCCTCCCAA GGAACAGCGA
181 GACCACAAGT CGGATGCAAC TGCAAGAGGG TTTATTGGAT ACACGGGTAC CCGGGCGACT
241 CAGTCAATCG GAGGACTGGC GCGCCGAGTG AGGGGTTGTG GGCTCTTTTA TTGAGCTCGG
301 GGAGCAGAAG CGCGCGAACA GAAGCGAGAA GCGAACTGAT TGGTTAGTTC AAATAAGGCA
361 CAGGGTCATT TCAGGTCCTT GGGGCACCCT GGAAACATCT GATGGTTCTC TAGAAACTGC
421 TGAGGGCTGG ACCGCATCTG GGGACCATCT GTTCTTGGCC CTGAGCCGGG GCAGGAACTG
481 CTTACCACAG ATATCCTGTT TGGCCCATAT TCAGCTGTTC CATCTGTTCC TGACCTTGAT
541 CTGAACTTTT CTATTCTCAG TTATGTATTT TTCCATGCCT TGCAAAATGG CGTTACTTAA
601 GCTAGCTTGC CAAACCTACA GGTGGGGTCT TTCATTCCCC CCTTTTTCTG GAGACTAAAT
661 AAAATCTTTT ATTTTATCGT CGACCACTGT GCTGGCGGCC GCTCGAGTTT AAATAGTACA
721 TAATGGATTT CCTTACGCGA AATACGGGCA GACATGGCCT GCCCGGTTAT TATTATTTTT
781 GACACCAGAC CAACTGGTAA TGGTAGCGAC CGGCGCTCAG CTGGAATATC ACCACTTTGT
841 ACAAGAAAGC TGGGTCGGCG CGCCCACCCT TTCACATGTG TGAGAGGGGC AGTGTGCCGT
901 TAATGGCCGT GCCGGGCACC GGGCCGCTCT GGTAGTGCTG GGACATGTGA AGTCTGCTGG
961 GGGCGGCGGG TTCCGGCACC TCGGCGCCGG GGAGATACAT GCTGATCATG TCCCGGAGGT
1021 CCCCGGCCTG GCAGGGCGCC CTGGAGTGGG AGGAAGAGGT AACCACAGGG GGGCTGGAGC
1081 TGGCCTCGGA CTTGACCACC GAACCCATGG AGCCAAGAGC CATGCCAGGG GTGCCCTGCT
1141 GCGAGTAGGA CATGCTGTAG GTGGGCGAGC CGTTCATGTA GGTCTGCGAG CTGGTCATGG
1201 AGTTGTACTG CAGGGCGCTC ACGTCGTAGC GGTGCATGGG CTGCATCTGC GCTGCGCCGT
1261 GCGCATTGAG GCCCGGGTGC TGCGGGTAGC CCAGCTGGTC CTGCATCATG CTGTAGCTGC
1321 CGTTGCTCCA GCCGTTCATG TGCGCGTAAC TGTCCATGCG CTGGTTCACG CCCGCGCCCA
1381 GGCCGGCGCC CACCCCGACC CCGCTCGCCA TGCTATTGCC GCCGGGGGCC AGCAGCCCGC
1441 CGGGCAGCGT GTACTTATCC TTCTTCATGA GCGTCTTGGT TTTCCGCCGG GGCCGGTATT
1501 TATAATCCGG GTGCTCCTTC ATGTGCAGCG CTCGCAGCCG CTTAGCCTCG TCGATGAACG
1561 GCCGCTTCTC CGTCTCCGAC AAAAGTTTCC ACTCGGCGCC CAGGCGCTTG CTGATCTCCG
1621 AGTTGTGCAT CTTGGGGTTC TCCTGGGCCA TCTTGCGCCG CTGCCCGCGG GACCACACCA
1681 TGAAGGCATT CATGGGCCGC TTGACGCGGT CCGGGCTGTT TTTCTGGTTG CCGCCGGCCG
1741 CCGCCGCGGT GGAGTTGCCG CCGCCGCCCC CCGAAGTTTG CTGCGGGCCC GGCGGCTTCA
1801 GCTCCGTCTC CATCATGTTG TACATGGTGA AGGGGGCGGC CGCGGAGCCT GCTTTTTTGT
1861 ACAAACTTGT GATTTCCCGT ACCACCACAC TGGGATCCTT AATTAACTAG CTAGATCCGG
1921 CAGTCTAGAG GATGGTCCAC CCCCGGGGTC GGCAGCCTTC ACGTGGGCGG CGTGTATCCA
1981 AGCTGCGATG CCGTCTACTT TGAGGGCGGT GGGGGTGGTC AGCAGGACTG TGTAAGGTCC
2041 TTTCCAGCGA GGTTCTAGGT TCTTAGTCTG GTGTCGGCGG ACCCACACTG TGTCGCCGAC
2101 TCGGTAAGGG TGAGGTACCA CCGGTCGGTC CAGTTGTTCT TGGTAGGCTG CCGCCAGAGG
2161 TCTCCAGACT TCGTGCTGGA CTAAGTAGAG AGCCTGTAAG TGAGCTTGGA GAGAGGGGCT
2221 GTTAGTAACT CTTGTCATGT CAGGGTCAGG GAAGTTTACA AGGGGCGGGG GTGCCCCATA
2281 TAAGATCTCA TATGGCCATA TGGGGGCGCC TAGAGAAGGA GTGAGGGCTG GATAAAGGGA
2341 GGATCGAGGC GGGGTCGAAC GAGGAGGTTC AAGGGGGAGA GACGGGGCGG ATGGAGGAAG
2401 AGGAGGCGGA GGCTTAGGGT GTACAAAGGG CTTGACCCAG GGAGGGGGGT CAAAAGCCAA
2461 GGCTTCCCAG GTCACGATGT AGGGGACCTG GTCTGGGTGT CCATGCGGGC CAGGTGAAAA
2521 GACCTTGATC TTAACCTGGG TGATGAGGTC TCGGTTAAAG GTGCCGTCTC GCGGCCATCC
2581 GACGTTAAAG GTTGGCCATT CTGCAGAGCA GAAGGTAACC CAACGTCTCT TCTTGACATC
2641 TACCGACTGG TTGTGAGCGA TCCGCTCGAC ATCTTTCCAG TGACCTAAGG TCAAACTTAA
2701 GGGAGTGGTA ACAGTCTGGC CCTAATTTTC AGACAAATAC AGAAACACAG TCAGACAGAG
2761 ACAACACAGA ACGATGCTGC AGCAGACAAG ACGCGCGGCG CGGCTTCGGT CCCAAACCGA
2821 AAGCAAAAAT TCAGACGGAG GCGGGAACTG TTTTAGGTTC TCGTCTCCTA CCAGAACCAC
2881 ATATCCCTCC TATTAGGGGG GTGCACCAAA GAGTCCAAAA CGATCGGGAT TTTTGGACTC
2941 AGGTCGGGCC ACAAAAACGG CCCCCGAAGT CCCTGGGACG TCTCCCAGGG TTGCGGCCGG
3001 GTGTTCCGAA CTCGTCAGTT CCACCACGGG TCCGCCAGAT ACAGAGCTAG TTAGCTAACT
3061 AGTACCGACG CAGGCGCATA AAATCAGTCA TAGACACTAG ACAATCGGAC AGACACAGAT
3121 AAGTTGCTGG CCAGCTTACC TCCCGGTGGT GGGTCGGTGG TCCCTGGGCA GGGGTCTCCC
3181 GATCCCGGAC GAGCCCCCAA ATGAAAGACC CCCGCTGACG GGTAGTCAAT CACTCAGAGG
3241 AGACCCTCCC AAGGAACAGC GAGACCACAA GTCGGATGCA ACTGCAAGAG GGTTTATTGG
3301 ATACACGGGT ACCCGGGCGA CTCAGTCAAT CGGAGGACTG GCGCGCCGAG TGAGGGGTTG
3361 TGGGCTCTTT TATTGAGCTC GGGGAGCAGA AGCGCGCGAA CAGAAGCGAG AAGCGAACTG
3421 ATTGGTTAGT TCAAATAAGG CACAGGGTCA TTTCAGGTCC TTGGGGCACC CTGGAAACAT
3481 CTGATGGTTC TCTAGAAACT GCTGAGGGCT GGACCGCATC TGGGGACCAT CTGTTCTTGG
3541 CCCTGAGCCG GGGCAGGAAC TGCTTACCAC AGATATCCTG TTTGGCCCAT ATTCAGCTGT
3601 TCCATCTGTT CTTGGCCCTG AGCCGGGGCA GGAACTGCTT ACCACAGATA TCCTGTTTGG
3661 CCCATATTCA GCTGTTCCAT CTGTTCCTGA CCTTGATCTG AACTTTTCTA TTCTCAGTTA
3721 TGTATTTTTC CATGCCTTGC AAAATGGCGT TACTTAAGCT AGCTTGCCAA ACCTACAGGT
3781 GGGGTCTTTC ATTATGCAGG TGGCACTTTT CGGGGAAATG TGCGCGGAAC CCCTATTTGT
3841 TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA GACAATAACC CTGATAAATG
3901 CTTCAATAAT ATTGAAAAAG GAAGAGTATG AGTATTCAAC ATTTCCGTGT CGCCCTTATT
3961 CCCTTTTTTG CGGCATTTTG CCTTCCTGTT TTTGCTCACC CAGAAACGCT GGTGAAAGTA
4021 AAAGATGCTG AAGATCAGTT GGGTGCACGA GTGGGTTACA TCGAACTGGA TCTCAACAGC
4081 GGTAAGATCC TTGAGAGTTT TCGCCCCGAA GAACGTTTTC CAATGATGAG CACTTTTAAA
4141 GTTCTGCTAT GTGGCGCGGT ATTATCCCGT ATTGACGCCG GGCAAGAGCA ACTCGGTCGC
4201 CGCATACACT ATTCTCAGAA TGACTTGGTT GAGTACTCAC CAGTCACAGA AAAGCATCTT
4261 ACGGATGGCA TGACAGTAAG AGAATTATGC AGTGCTGCCA TAACCATGAG TGATAACACT
4321 GCGGCCAACT TACTTCTGAC AACGATCGGA GGACCGAAGG AGCTAACCGC TTTTTTGCAC
4381 AACATGGGGG ATCATGTAAC TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA TGAAGCCATA
4441 CCAAACGACG AGCGTGACAC CACGATGCCT GTAGCAATGG CAACAACGTT GCGCAAACTA
4501 TTAACTGGCG AACTACTTAC TCTAGCTTCC CGGCAACAAT TAATAGACTG GATGGAGGCG
4561 GATAAAGTTG CAGGACCACT TCTGCGCTCG GCCCTTCCGG CTGGCTGGTT TATTGCTGAT
4621 AAATCTGGAG CCGGTGAGCG TGGGTCTCGC GGTATCATTG CAGCACTGGG GCCAGATGGT
4681 AAGCCCTCCC GTATCGTAGT TATCTACACG ACGGGGAGTC AGGCAACTAT GGATGAACGA
4741 AATAGACAGA TCGCTGAGAT AGGTGCCTCA CTGATTAAGC ATTGGTAACT GTCAGACCAA
4801 GTTTACTCAT ATATACTTTA GATTGATTTG CGGCCGGCCG CAAAAGGATC TAGGTGAAGA
4861 TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT
4921 CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT
4981 GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC
5041 TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTTC
5101 TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC
5161 TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG
5221 GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT
5281 CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG
5341 AGCTATGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG
5401 GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT
5461 ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG
5521 GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT
5581 GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA
5641 TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT
5701 CAGTGAGCGA GGAAGCGGAA G
//
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