| 质粒名称: | pN565 |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 49990 |
| 存储实验室: | Christopher Voigt |
|
载体骨架基本信息 |
|
| 载体名称: | pIncW |
| 载体出品公司: | -- |
| 空载体大小: | -- |
| 完整质粒大小: | 9856 bp |
| 载体修饰: | 质粒pIncW（pSa，SpR）通过缺失osa、nuc1、Tn21整合酶基因和ORF18从pEXT21（pSa、SpR）产生（Temme等人，PNAS 2012，PMID 22509035）。 |
| 载体类型: | 细菌表达，合成生物学 |
| 筛选标记: | -- |
| 载体抗性: | 大观霉素，50μg/mL |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH10B |
| 拷贝数: | 低拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | T7 RNAP* |
| 物种来源: | Bacteriophage T7 |
| 插入大小: | -- |
| 基因突变: | R632S |
| GenBank编号 | -- |
| 启动子: | pTac-symmetric |
| 标签/融合蛋白: | umuD降级标签（主干上的N终端） |
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | -- |
| 3’克隆位点: | -- |
| 5’测序引物 | aaggcgcactcccgttctgg |
| 3’测序引物 | attaccgcctttgagtgagc |
|
文献引用方法 |
|
| 材料和方法部分: | pN565 was a gift from Christopher Voigt (Addgene plasmid # 49990 ; http://n2t.net/addgene:49990 ; RRID:Addgene_49990) |
| 参考文献部分: | Design of orthogonal genetic switches based on a crosstalk map of sigmas, anti-sigmas, and promoters. Rhodius VA, Segall-Shapiro TH, Sharon BD, Ghodasara A, Orlova E, Tabakh H, Burkhardt DH, Clancy K, Peterson TC, Gross CA, Voigt CA. Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58. 10.1038/msb.2013.58 PubMed 24169405 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pN565 9856 bp ds-DNA circular SYN 09-FEB-2023
DEFINITION expresses an attenuated T7 RNAP under an isopropyl
beta-d-1-thiogalactopyranoside (IPTG) inducible control.
ACCESSION .
VERSION .
KEYWORDS pN565
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9856)
AUTHORS Rhodius VA, Segall-Shapiro TH, Sharon BD, Ghodasara A, Orlova E,
Tabakh H, Burkhardt DH, Clancy K, Peterson TC, Gross CA, Voigt CA
TITLE Design of orthogonal genetic switches based on a crosstalk map of
sigmas, anti-sigmas, and promoters.
JOURNAL Mol Syst Biol. 2013 Oct 29;9:702. doi: 10.1038/msb.2013.58.
PUBMED 24169405
REFERENCE 2 (bases 1 to 9856)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Feb 9, 2023 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..9856
/organism="synthetic DNA construct"
/mol_type="other DNA"
misc_feature 2776..2796
/label=BioBrick suffix
/note="universal suffix for all parts"
terminator 2832..2863
/label=tonB terminator
/note="bidirectional E. coli tonB-P14 transcription
terminator"
CDS 3725..4696
/codon_start=1
/gene="repA"
/product="replication protein of plasmid pSa"
/label=RepA
/translation="MPKNNKAPGHRINEIIKTSLALEMEDAREAGLVGYMARCLVQATM
PHTDPKTSYFERTNGIVTLSIMGKPSIGLPYGSMPRTLLAWICTEAVRTKDPVLNLGRS
QSEFLQRLGMHTDGRYTATLRNQAQRLFSSMISLAGEQGNDFGIENVVIAKRAFLFWNP
KRPEDRALWDSTLTLTGDFFEEVTRSPVPIRIDYLHALRQSPLAMDIYTWLTYRVFLLR
AKGRPFVQIPWVALQAQFGSSYGSRARNSPELDDKARERAERAALASFKYNFKKRLREV
LIVYPEASDCIEDDGECLRIKSTRLHVTRAPGKGARIGPPPT"
rep_origin 4733..5168
/label=pSa ori
/note="origin of replication from bacterial plasmid pSa"
CDS 6843..7634
/codon_start=1
/gene="aadA"
/product="aminoglycoside adenylyltransferase (Murphy,
1985)"
/label=SmR
/note="confers resistance to spectinomycin and
streptomycin"
/translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
VILEARQAYLGQEEDRLASRTDQLEEFVHYVKGEITKVVGK"
terminator 7911..7940
/label=T3Te terminator
/note="phage T3 early transcription terminator"
CDS complement(8043..9125)
/codon_start=1
/gene="lacI"
/product="lac repressor"
/label=lacI
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
/translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVYDQTPINSIIFSH
EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
ALADSLMQLARQVSRLESGQ"
primer_bind 9087..9106
/label=LacI-R
/note="LacI, reverse primer"
promoter complement(9126..9203)
/gene="lacI (mutant)"
/label=lacIq promoter
/note="In the lacIq allele, a single base change in the
promoter boosts expression of the lacI gene about 10-fold."
promoter 9722..9750
/label=tac promoter
/note="strong E. coli promoter; hybrid between the trp and
lac UV5 promoters"
protein_bind 9756..9775
/label=lac operator (symmetric)
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG). The
symmetric lac operator was optimized for tight binding of
lac repressor."
ORIGIN
1 gtgttgttta tcaagcctgc ggatctccgc gaaattgtga cttttccgct atttagcgat
61 cttgttcagt gtggctttcc ttcaccggca gcagattacg ttgaacagcg catcgatctg
121 ggtggcaaca cgattaacat cgctaagaac gacttctctg acatcgaact ggctgctatc
181 ccgttcaaca ctctggctga ccattacggt gagcgtttag ctcgcgaaca gttggccctt
241 gagcatgagt cttacgagat gggtgaagca cgcttccgca agatgtttga gcgtcaactt
301 aaagctggtg aggttgcgga taacgctgcc gccaagcctc tcatcactac cctactccct
361 aagatgattg cacgcatcaa cgactggttt gaggaagtga aagctaagcg cggcaagcgc
421 ccgacagcct tccagttcct gcaagaaatc aagccggaag ccgtagcgta catcaccatt
481 aagaccactc tggcttgcct aaccagtgct gacaatacaa ccgttcaggc tgtagcaagc
541 gcaatcggtc gggccattga ggacgaggct cgcttcggtc gtatccgtga ccttgaagct
601 aagcacttca agaaaaacgt tgaggaacaa ctcaacaagc gcgtagggca cgtctacaag
661 aaagcattta tgcaagttgt cgaggctgac atgctctcta agggtctact cggtggcgag
721 gcgtggtctt cgtggcataa ggaagactct attcatgtag gagtacgctg catcgagatg
781 ctcattgagt caaccggaat ggttagctta caccgccaaa atgctggcgt agtaggtcaa
841 gactctgaga ctatcgaact cgcacctgaa tacgctgagg ctatcgcaac ccgtgcaggt
901 gcgctggctg gcatctctcc gatgttccaa ccttgcgtag ttcctcctaa gccgtggact
961 ggcattactg gtggtggcta ttgggctaac ggtcgtcgtc ctctggcgct ggtgcgtact
1021 cacagtaaga aagcactgat gcgctacgaa gacgtttaca tgcctgaggt gtacaaagcg
1081 attaacattg cgcaaaacac cgcatggaaa atcaacaaga aagtcctagc ggtcgccaac
1141 gtaatcacca agtggaagca ttgtccggtc gaggacatcc ctgcgattga gcgtgaagaa
1201 ctcccgatga aaccggaaga catcgacatg aatcctgagg ctctcaccgc gtggaaacgt
1261 gctgccgctg ctgtgtaccg caaggacaag gctcgcaagt ctcgccgtat cagccttgag
1321 ttcatgcttg agcaagccaa taagtttgct aaccataagg ccatctggtt cccttacaac
1381 atggactggc gcggtcgtgt ttacgctgtg tcaatgttca acccgcaagg taacgatatg
1441 accaaaggac tgcttacgct ggcgaaaggt aaaccaatcg gtaaggaagg ttactactgg
1501 ctgaaaatcc acggtgcaaa ctgtgcgggt gtcgacaagg ttccgttccc tgagcgcatc
1561 aagttcattg aggaaaacca cgagaacatc atggcttgcg ctaagtctcc actggagaac
1621 acttggtggg ctgagcaaga ttctccgttc tgcttccttg cgttctgctt tgagtacgct
1681 ggggtacagc accacggcct gagctataac tgctcccttc cgctggcgtt tgacgggtct
1741 tgctctggca tccagcactt ctccgcgatg ctccgagatg aggtaggtgg tcgcgcggtt
1801 aacttgcttc ctagtgaaac cgttcaggac atctacggga ttgttgctaa gaaagtcaac
1861 gagattctac aagcagacgc aatcaatggg accgataacg aagtagttac cgtgaccgat
1921 gagaacactg gtgaaatctc tgagaaagtc aagctgggca ctaaggcact ggctggtcaa
1981 tggctggctt acggtgttac tcgcagtgtg actaagagtt cagtcatgac gctggcttac
2041 gggtccaaag agttcggctt ccgtcaacaa gtgctggaag ataccattca gccagctatt
2101 gattccggca agggtctgat gttcactcag ccgaatcagg ctgctggata catggctaag
2161 ctgatttggg aatctgtgag cgtgacggtg gtagctgcgg ttgaagcaat gaactggctt
2221 aagtctgctg ctaagctgct ggctgctgag gtcaaagata agaagactgg agagattctt
2281 cgcaagcgtt gcgctgtgca ttgggtaact cctgatggtt tccctgtgtg gcaggaatac
2341 aagaagccta ttcagacgcg cttgaacctg atgttcctcg gtcagttccg cttacagcct
2401 accattaaca ccaacaaaga tagcgagatt gatgcacaca aacaggagtc tggtatcgct
2461 cctaactttg tacacagcca agacggtagc caccttcgta agactgtagt gtgggcacac
2521 gagaagtacg gaatcgaatc ttttgcactg attcacgact ccttcggtac gattccggct
2581 gacgctgcga acctgttcaa agcagtgcgc gaaactatgg ttgacacata tgagtcttgt
2641 gatgtactgg ctgatttcta cgaccagttc gctgaccagt tgcacgagtc tcaattggac
2701 aaaatgccag cacttccggc taaaggtaac ttgaacctcc gtgacatctt agagtcggac
2761 ttcgcgttcg cgtaatacta gtagcggccg ctgcaggagt cactaagggt tagttagtta
2821 gattagcaga aagtcaaaag cctccgaccg gaggcttttg actaaaactt cccttggggt
2881 tatcattggg gctcactcaa aggcggtaat cagataaaaa aaatccttag ctttcgctaa
2941 ggatgatttc tgctagagct gtcagaccag agctccaaac caacgtttta tctataccgc
3001 tacagggtat ttaattccta tttaatctgc gctagaatga ggcatgttta accgaatctg
3061 acgttttccc tgcaaatgcc aaaatactat gcctatctcc gggtttcgcg tgacggccaa
3121 gacccggaaa accaaaaata cggtttgctc gaatacgcga acgccaaagg cttcgcgccg
3181 ctacagatcg aggaagaaat tgccagcaga gcaaaggact ggcgcaagcg caagctcgga
3241 gcaatcatcg aaaaggccga gcgtggcgac gtgctactga cgccggagat tacgcgcatt
3301 gccggttccg ccctcgccgc cttggaaatt ctcaaagcgg cgagcgagcg cggcctaatc
3361 gtccatgtga ccaaacagaa gatcatcatg gacggcagcc tacaaagcga catcatggca
3421 accgtgcttg gcttggctgc acagatcgag cggcatttca ttcaggcacg taccaccgag
3481 gcgctacaag tcgccagaga gcgcggcaag acgctcgggc gacccaaggg cagcaaatcg
3541 agcgccttga agctggacag ccgtattgat gaagtacagg catacgtgaa ccttggcttg
3601 ccgcaaagtc gcgcagccga gttgttaggc gtcagccctc acaccttgcg cctgttcatc
3661 aaacgccgga acatcaaacc cacaaacact agaccaacca tcaccatgcc ggggagggaa
3721 caacatgcct aagaacaaca aagcccccgg ccatcgtatc aacgagatca tcaagacgag
3781 cctcgcgctc gaaatggagg atgcccgcga agctggctta gtcggctaca tggcccgttg
3841 ccttgtgcaa gcgaccatgc cccacaccga ccccaagacc agctactttg agcgcaccaa
3901 tggcatcgtc accttgtcga tcatgggcaa gccgagcatc ggcctgccct acggttctat
3961 gccgcgcacc ttgcttgctt ggatatgcac cgaggccgtg cgaacgaaag accccgtgtt
4021 gaaccttggc cggtcgcaat cggaatttct acaaaggctc ggaatgcaca ccgatggccg
4081 ttacacggcc acccttcgca atcaggcgca acgcctgttt tcatccatga tttcgcttgc
4141 cggcgagcaa ggcaatgact tcggcattga gaacgtcgtc attgccaagc gcgcttttct
4201 attctggaat cccaagcggc cagaagatcg ggcgctatgg gatagcaccc tcaccctcac
4261 aggcgatttc ttcgaggaag tcacccgctc accggttcct atccgaatcg actacctgca
4321 tgccttgcgg cagtctccgc ttgcgatgga catttacacg tggctgacct atcgcgtgtt
4381 cctgttgcgg gccaagggcc gccccttcgt gcaaatccct tgggtcgccc tgcaagcgca
4441 attcggctca tcctatggca gccgcgcacg caactcgccc gaactggacg ataaggcccg
4501 agagcgggca gagcgggcag cactcgccag cttcaaatac aacttcaaaa agcgcctacg
4561 cgaagtgttg attgtctatc ccgaggcaag cgactgcatc gaagatgacg gcgaatgcct
4621 gcgcatcaaa tccacacgcc tgcatgtcac ccgcgcaccc ggcaagggcg ctcgcatcgg
4681 cccccctccg acttgaccag gccaacgcta cgcttggctt ggtcaagcct tcccatccaa
4741 cagcccgccg tcgagcgggc ttttttatcc ccggaagcct gtggatagag ggtagttatc
4801 cacgcgaaac cgctaatgcc ccgcaaagcc ttgattcacg gggctttccg gcccgctcca
4861 aaaactatcc acgtgaaatc gctaatcagg gtacgtgaaa tcgctaatcg gagtacgtga
4921 aatcgctaat aaggtcacgt gaaatcgcta atcaaaaagg cacgtgagaa cgctaatagc
4981 cctttcagat caacagcttg caaacacccc tcgctccggc aagtagttac agcaagtagt
5041 atgttcaatt agcttttcaa ttatgaatat atatatcaat tattggtcgc ccttggcttg
5101 tggacaatgc gctacgcgca ccggctccgc ccgtggacaa ccgcaagcgg ttgcccaccg
5161 tcgagcgcct ttgcccacaa cccggcggcc gcaacagatc gttttataaa tttttttttt
5221 tgaaaaagaa aaagcccgaa aggcggcaac ctctcgggct tctggatttc cgatcaacgc
5281 aggagtcgtt cggaaagtag ctgttccaga attataggcg cagagacacc agattccaag
5341 atggctctgt taaattgttg tagtatgtag tatcatacaa catactacag tacagaggcc
5401 cgcaagaatg gcaatcacta aacaagacat ttggcgagca gccgacgaac tggacgccga
5461 aggcatccgg cccactttgg ccgccgtgcg caagaaactc ggaagcggta gcttcacaac
5521 catttccgat gcaatggctg aatggaaaaa ccgcaagacc gccaccctgc cctcatcaga
5581 cccattgccg gttgcagtca acgagcatct tgccgagctt ggcaatgcgc tatgggctat
5641 cgccctggcg cacgccaacg cccggtttga cgaagatcgg aaacagatcg aggccgacaa
5701 agcggccatc agccagcagc ttgccgaagc aatcgaacta gccgacacct tcacccgcga
5761 aaacgaccag ctccgcgaac gagtgaatca gctcgaacct atggaacgcg agcgcgacaa
5821 gctggccgac cagcttgccg aagtgaaacg ccgcagcggc gaagaactaa accgctgcat
5881 ggaaaagctc acccaacgcg ataacgaggc tatcgaggcc cgcaaacagg ccaaggaggc
5941 catcgagcgc gccgccagtc tgcaaggtca ggtggaagcc ctcaaagagc aggtcgccaa
6001 tctcacagcc gtcttgaaaa caggaggcaa acaatgaaaa gcgcccttgc cgcccttcgc
6061 gcggtcgcgg ccgctgtcgt cctaatcgtc agtgtgcccg cttgggccga cttccggggt
6121 gaagtcgtcc gaatccttga cggtgacact atcgacgttt tggtgaaccg tcagaccatc
6181 cgcgtgagat tggccgatat tgacgcaccg gaaagcggcc aagccttcgg ctcccgtgct
6241 cgccaacggc tcgccgactt gacctttcgc caagaggttc aagtgaccga aaaagaggtt
6301 gatcggtatg gccgcactct tggggtcgtt tacgcgccgt tgcaataccc cggcggccaa
6361 acacaactca ccaacatcaa tgcgatcatg gttcaagaag gcatggcctg ggcttaccgt
6421 tattacggca aaccaaccga cgcgcagatg tacgagtatg aaaaagaggc ccgccgccaa
6481 cggctcggcc tttggtcaga cccgaatgct caggagcctt ggaaatggcg tcgcgcctcg
6541 aaaaatgcca cgaactgaca ccgggcacgc cccttgttcg acgcgccgca ggcacgtcga
6601 atttaccgcc gggacgcccc tcgtcccgac acttccagat cgccatagcg cacagcgcct
6661 cgagcggtgg taacggcgca gtggcggttt tcatggcttg ttatgactgt ttttttgggg
6721 tacagtctat gcctcgggca tccaagcagc aagcgcgtta cgccgtgggt cgatgtttga
6781 tgttatggag cagcaacgat gttacgcagc agggcagtcg ccctaaaaca aagttaaaca
6841 tcatgaggga agcggtgatc gccgaagtat cgactcaact atcagaggta gttggcgtca
6901 tcgagcgcca tctcgaaccg acgttgctgg ccgtacattt gtacggctcc gcagtggatg
6961 gcggcctgaa gccacacagt gatattgatt tgctggttac ggtgaccgta aggcttgatg
7021 aaacaacgcg gcgagctttg atcaacgacc ttttggaaac ttcggcttcc cctggagaga
7081 gcgagattct ccgcgctgta gaagtcacca ttgttgtgca cgacgacatc attccgtggc
7141 gttatccagc taagcgcgaa ctgcaatttg gagaatggca gcgcaatgac attcttgcag
7201 gtatcttcga gccagccacg atcgacattg atctggctat cttgctgaca aaagcaagag
7261 aacatagcgt tgccttggta ggtccagcgg cggaggaact ctttgatccg gttcctgaac
7321 aggatctatt tgaggcgcta aatgaaacct taacgctatg gaactcgccg cccgactggg
7381 ctggcgatga gcgaaatgta gtgcttacgt tgtcccgcat ttggtacagc gcagtaaccg
7441 gcaaaatcgc gccgaaggat gtcgctgccg actgggcaat ggagcgcctg ccggcccagt
7501 atcagcccgt catacttgaa gctagacagg cttatcttgg acaagaagaa gatcgcttgg
7561 cctcgcgcac agatcagttg gaagaatttg tccactacgt gaaaggcgag atcaccaagg
7621 tagtcggcaa ataatgtcta acaattcgtt caagccgacg ccgcttcgcg gcgcggctta
7681 actcaagcgt tagatgcact aagcacataa ttgctcacag ccaaactatc aggtcaagtc
7741 tgcttttatt atttttaagc gtgcataata agccctacac aaatggtacc ctccggcaaa
7801 aaaacgggca aggtgtcacc accctgccct ttttctttaa aaccgaaaag attacttcgc
7861 gtttgccacc tgacgtctaa gaaaaggaat attcagcaat ttgcccgtgc cgaagaaagg
7921 cccacccgtg aaggtgagcc agtgagttga ttgctacgta attagttagt tagcccttag
7981 tgactcgaat tcttggcagc ttctagagca attcgcgcta acttacatta attgcgttgc
8041 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
8101 aacgcgcggg gagaggcggt ttgcgtattg ggcgccaggg tggtttttct tttcaccagt
8161 gagacgggca acagctgatt gcccttcacc gcctggccct gagagagttg cagcaagcgg
8221 tccacgctgg tttgccccag caggcgaaaa tcctgtttga tggtggttaa cggcgggata
8281 taacatgagc tgtcttcggt atcgtcgtat cccactaccg agatatccgc accaacgcgc
8341 agcccggact cggtaatggc gcgcattgcg cccagcgcca tctgatcgtt ggcaaccagc
8401 atcgcagtgg gaacgatgcc ctcattcagc atttgcatgg tttgttgaaa accggacatg
8461 gcactccagt cgccttcccg ttccgctatc ggctgaattt gattgcgagt gagatattta
8521 tgccagccag ccagacgcag acgcgccgag acagaactta atgggcccgc taacagcgcg
8581 atttgctggt gacccaatgc gaccagatgc tccacgccca gtcgcgtacc gtcttcatgg
8641 gagaaaataa tactgttgat gggtgtctgg tcatagacat caagaaataa cgccggaaca
8701 ttagtgcagg cagcttccac agcaatggca tcctggtcat ccagcggata gttaatgatc
8761 agcccactga cgcgttgcgc gagaagattg tgcaccgccg ctttacaggc ttcgacgccg
8821 cttcgttcta ccatcgacac caccacgctg gcacccagtt gatcggcgcg agatttaatc
8881 gccgcgacaa tttgcgacgg cgcgtgcagg gccagactgg aggtggcaac gccaatcagc
8941 aacgactgtt tgcccgccag ttgttgtgcc acgcggttgg gaatgtaatt cagctccgcc
9001 atcgccgctt ccactttttc ccgcgttttc gcagaaacgt ggctggcctg gttcaccacg
9061 cgggaaacgg tctgataaga gacaccggca tactctgcga catcgtataa cgttactggt
9121 ttcacattca ccaccctgaa ttgactctct tccgggcgct atcatgccat accgcgaaag
9181 gttttgcacc attcgatggt gtcaacgtaa atgcatgccg cttcgccttc gcgcgcgaat
9241 tggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag
9301 tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca
9361 tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct
9421 gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc
9481 gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgagatccgg
9541 gcttatcgac tgcacggtgc accaatgctt ctggcgtcag gcagccatcg gaagctgtgg
9601 tatggctgtg caggtcgtaa atcactgcat aattcgtgtc gctcaaggcg cactcccgtt
9661 ctggataatg ttttttgcgc cgacatcata acggttctgg caaatattct gaaatgagct
9721 gttgacaatt aatcatcggc tcgtataatg tgtggaattg tgagcgctca caatttacta
9781 gatgcagttt tattctctcg ccagcactgt aataggcact aatatccaaa ccagtagctc
9841 aattggagtc gtctat
//
