| 质粒名称: | pREDSIX |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 74103 |
| 存储实验室: | Hans-Martin Fischer |
|
载体骨架基本信息 |
|
| 载体名称: | pUCBM21 |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | -- |
| 完整质粒大小: | -- |
| 载体修饰: | 见Ledermann等人，附录。环境微生物学，2016年 |
| 载体类型: | 结合细菌载体 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | mCherry |
| 物种来源: | Synthetic |
| 插入大小: | 708 bp |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | -- |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pREDSIX was a gift from Hans-Martin Fischer (Addgene plasmid # 74103 ; http://n2t.net/addgene:74103 ; RRID:Addgene_74103) |
| 参考文献部分: | Versatile vectors for efficient mutagenesis of Bradyrhizobium diazoefficiens and other Alphaproteobacteria. Ledermann R, Strebel S, Kampik C, Fischer HM. Appl Environ Microbiol. 2016 Feb 26. pii: AEM.04085-15. 10.1128/AEM.04085-15 PubMed 26921431 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pREDSIX 5348 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION mCherry-encoding vector for marker replacement mutagenesis in
Bradyrhizobium diazoefficiens and other Alphaproteobacteria.
ACCESSION .
VERSION .
KEYWORDS pREDSIX
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5348)
AUTHORS Ledermann R, Strebel S, Kampik C, Fischer HM
TITLE Versatile vectors for efficient mutagenesis of Bradyrhizobium
diazoefficiens and other Alphaproteobacteria.
JOURNAL Appl Environ Microbiol. 2016 Feb 26. pii: AEM.04085-15.
PUBMED 26921431
REFERENCE 2 (bases 1 to 5348)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..5348
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind complement(73..90)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(244..832)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(324..343)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(1757..2128)
/codon_start=1
/gene="traJ"
/product="oriT-recognizing protein"
/label=traJ
/translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
KQDELGKVMMGVVRPRAEP"
oriT 2161..2270
/note="incP origin of transfer"
primer_bind complement(3216..3235)
/label=pBRrevBam
/note="pBR322 vectors, tet region, downstream of BamHI,
reverse primer"
CDS complement(3372..4232)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 3995..4014
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(4233..4337)
/gene="bla"
/label=AmpR promoter
CDS 4620..5327
/codon_start=1
/product="monomeric derivative of DsRed fluorescent protein
(Shaner et al., 2004)"
/label=mCherry
/note="mammalian codon-optimized"
/translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
EGRHSTGGMDELYK"
primer_bind complement(4765..4783)
/label=mCherry-R
/note="mCherry, reverse primer"
primer_bind 5033..5052
/label=mCherry-F
/note="mCherry, forward primer"
ORIGIN
1 ctgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
61 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
121 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
181 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
241 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
301 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
361 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
421 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
481 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
541 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
601 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
661 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
721 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
781 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
841 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
901 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
961 taaatcaatc taaagtatat atgagtaaac ttggtctgac aggtttaaac tctagacaat
1021 tgcctaggat gcatgctagc cttaagattt aaatgttaat taatccggtt tattgactac
1081 cggaagcagt gtgaccgtgt gcttctcaaa tgcctgaggc cagtttgctc aggctctccc
1141 cgtggaggta ataattgacg atatgatcca gccgaccagg ctttccacgc ccgcgtgccg
1201 ctccatgtcg ttcgcgcggt tctcggaaac gcgctgccgc gtttcgtgat tgtcacgctc
1261 aagcccgtag tcccgttcga gcgtcgcgca gaggtcagcg agggcgcggt aggcccgata
1321 cggctcatgg atggtgtttc gggtcgggtg aatcttgttg atggcgatat ggatgtgcag
1381 gttgtcggtg tcgtgatgca cggcactgac gcgctgatgc tcggcgaagc caagcccagc
1441 gcagatgcgg tcctcaatcg cgcgcaacgt ctccgcgtcg ggcttctctc ccgcgcggaa
1501 gctaaccagc aggtgatagg tcttgtcggc ctcggaacgg gtgttgccgt gctgggtcgc
1561 catcacctcg gccatgacag cgggcagggt gtttgcctcg cagttcgtga cgcgcacgtg
1621 acccaggcgc tcggtcttgc cttgctcgtc ggtgatgtac ttcaccagct ccgcgaagtc
1681 gctcttcttg atggagcgca tggggacgtg cttggcaatc acgcgcaccc cccggccgtt
1741 ttagcggcta aaaaagtcat ggctctgccc tcgggcggac cacgcccatc atgaccttgc
1801 caagctcgtc ctgcttctct tcgatcttcg ccagcagggc gaggatcgtg gcatcaccga
1861 accgcgccgt gcgcgggtcg tcggtgagcc agagtttcag caggccgccc aggcggccca
1921 ggtcgccatt gatgcgggcc agctcgcgga cgtgctcata gtccacgacg cccgtgattt
1981 tgtagccctg gccgacggcc agcaggtagg ccgacaggct catgccggcc gccgccgcct
2041 tttcctcaat cgctcttcgt tcgtctggaa ggcagtacac cttgataggt gggctgccct
2101 tcctggttgg cttggtttca tcagccatcc gcttgccctc atctgttacg ccggcggtag
2161 ccggccagcc tcgcagagca ggattcccgt tgagcaccgc caggtgcgaa taagggacag
2221 tgaagaagga acacccgctc gcgggtgggc ctacttcacc tatcctgccc ggctgacgcc
2281 gttggataca ccaaggaaag tctacacgaa ccctttggca aaatcctgta tatcgtgcga
2341 aaaaggatgg atataccgaa aaaatcgcta taatgacccc gaagcagggt tatgcagcgg
2401 aaaagcgctg cttccctgct gttttgtgga atatctaccg actggaaaca ggcaaatgca
2461 ggaaattact gaactgaggg gacaggcgag agacgatgcc aaagagctac accgacgagc
2521 tggccgagtg ggttgaatcc cgcgcggcca agaagcgccg gcgtgatgag gctgcggttg
2581 cgttcctggc ggtgagggcg gatgtcgagg cggcgttagc gtccggctat gcgctcgtca
2641 ccatttggga gcacatgcgg gaaacgggga aggtcaagtt ctcctacgag acgttccgct
2701 cgcacgccag gcggcacatc aaggccaagc ccgccgatgt gcccgcaccg caggccaagg
2761 ctgcggaacc cgcgccggca cccaagacgc cggagccacg gcggccgaag caggggggca
2821 aggctgaaaa gccggccccc gctgcggccc cgaccggctt caccttcaac ccaacaccgg
2881 acaaaaagga tctactgtaa tggcgaaaat tcacatggtt ttgcagggca agggcggggt
2941 cggcaagtcg gccatcgccg cgatcattgc gcagtacaag atggacaagg ggcagacacc
3001 cttgtgcatc gacaccgacc cggtgaacgc gacgttcgag ggctacaagg ccctgaacgt
3061 ccgccggctg aacatcatgg ccggcgacga aattaactcg cgcaacttcg acaccctggt
3121 cgagctgatt gcgccgacca aggatgacgt ggtgatcctc tacgccggac gcatcgtggc
3181 cggcatcacc ggcgccacag gtgcggttgc tggcgcctat atcgccgaca tcaccgatgg
3241 ggaagatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg
3301 caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca
3361 gcctcgcgtc gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
3421 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
3481 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
3541 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
3601 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
3661 agtttgcgca acgttgttgc cattgctgca ggcatcgtgg tgtcacgctc gtcgtttggt
3721 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
3781 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
3841 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
3901 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
3961 cgaccgagtt gctcttgccc ggcgtcaaca cgggataata ccgcgccaca tagcagaact
4021 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
4081 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
4141 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
4201 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
4261 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
4321 caaatagggg ttccgcgcac atttccaatt cgcccttagt gaagacaaag gtaagcttgc
4381 tcgatctcgg cgagcgccgc ggcggcgccc cggaggcgca gcggctctac gagcaggtcg
4441 gcgaggccgc gtcctgaccc ctcattcggc gcgatccgcc ctgccggcaa acctgccgct
4501 tgatcatcgt cgcacaagcc tctaatcggt ccgcgccttt acgcccggct tatggccggt
4561 cccgcggtag gattgcacga agcttagatc ttgactagaa ataagaagga gatataatta
4621 tggtgagcaa gggcgaggag gataacatgg ccatcatcaa ggagttcatg cgcttcaagg
4681 tgcacatgga gggctccgtg aacggccacg agttcgagat cgagggcgag ggcgagggcc
4741 gcccctacga gggcacccag accgccaagc tgaaggtgac caagggtggc cccctgccct
4801 tcgcctggga catcctgtcc cctcagttca tgtacggctc caaggcctac gtgaagcacc
4861 ccgccgacat ccccgactac ttgaagctgt ccttccccga gggcttcaag tgggagcgcg
4921 tgatgaactt cgaggacggc ggcgtggtga ccgtgaccca ggactcctcc ctgcaggacg
4981 gcgagttcat ctacaaggtg aagctgcgcg gcaccaactt cccctccgac ggccccgtaa
5041 tgcagaagaa gaccatgggc tgggaggcct cctccgagcg gatgtacccc gaggacggcg
5101 ccctgaaggg cgagatcaag cagaggctga agctgaagga cggcggccac tacgacgctg
5161 aggtcaagac cacctacaag gccaagaagc ccgtgcagct gcccggcgcc tacaacgtca
5221 acatcaagtt ggacatcacc tcccacaacg aggactacac catcgtggaa cagtacgaac
5281 gcgccgaggg ccgccactcc accggcggca tggacgagct gtacaaggaa ttgaagctta
5341 gatcttga
//
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