| 质粒名称: | pSFV3-lacZ |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 92074 |
| 存储实验室: | Henrik Garoff |
|
载体骨架基本信息 |
|
| 载体名称: | pSFV3 |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | -- |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | LacZ 表达质粒，用于哺乳动物细胞的径流转录和 RNA 转染 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | -- |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | lacZ |
| 物种来源: | -- |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | -- |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pSFV3-lacZ was a gift from Henrik Garoff (Addgene plasmid # 92074 ; http://n2t.net/addgene:92074 ; RRID:Addgene_92074) |
| 参考文献部分: | A new generation of animal cell expression vectors based on the Semliki Forest virus replicon. Liljestrom P, Garoff H. Biotechnology (N Y). 1991 Dec;9(12):1356-61. PubMed 1370252 |
买家导航
pSFV3lacZ载体基本信息
pSFV3lacZ载体图谱质粒图谱和多克隆位点信息
pSFV3lacZ载体简介
pSFV3lacZ载体序列
LOCUS pSFV3-lacZ 14110 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION This is an expression plasmid for production of recombinant genomic
SFV RNA encoding beta-galactosidase..
ACCESSION .
VERSION .
KEYWORDS pSFV3-lacZ
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 14110)
AUTHORS Liljestrom P, Garoff H
TITLE A new generation of animal cell expression vectors based on the
Semliki Forest virus replicon.
JOURNAL Biotechnology (N Y). 1991 Dec;9(12):1356-61.
PUBMED 1370252
REFERENCE 2 (bases 1 to 14110)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..14110
/organism="synthetic DNA construct"
/mol_type="other DNA"
CDS 7430..10477
/codon_start=1
/gene="lacZ"
/product="beta-galactosidase"
/label=lacZ
/translation="VVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRS
LNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPP
FVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQDSRLPSEF
DLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATR
FNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIIDERGGYADRV
TLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIENGLLLLNGKP
LLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGL
YVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNESGHGA
NHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLS
LPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSLIKYDENGNP
WSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQTIEVTSEYL
FRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQ
PNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQ
SGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWKAAGHYQAEA
ALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDVEVASDTPHP
ARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPYVFPSENGLR
CGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDD
SWSPSVSAEFQLSAGRYHYQLVWCQK"
primer_bind complement(7432..7454)
/label=M13/pUC Forward
/note="In lacZ gene"
primer_bind complement(11409..11426)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(11580..12168)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(11660..11679)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(12339..13199)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 12962..12981
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(13200..13304)
/gene="bla"
/label=AmpR promoter
primer_bind 13372..13390
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
primer_bind complement(13428..13450)
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind 13550..13569
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 13784..13803
/label=pBRrevBam
/note="pBR322 vectors, tet region, downstream of BamHI,
reverse primer"
promoter 14094..2
/label=SP6 promoter
/note="promoter for bacteriophage SP6 RNA polymerase"
primer_bind 14094..1
/label=SP6
/note="SP6 promoter, forward primer"
ORIGIN
1 gatggcggat gtgtgacata cacgacgcca aaagattttg ttccagctcc tgccacctcc
61 gctacgcgag agattaacca cccacgatgg ccgccaaagt gcatgttgat attgaggctg
121 acagcccatt catcaagtct ttgcagaagg catttccgtc gttcgaggtg gagtcattgc
181 aggtcacacc aaatgaccat gcaaatgcca gagcattttc gcacctggct accaaattga
241 tcgagcagga gactgacaaa gacacactca tcttggatat cggcagtgcg ccttccagga
301 gaatgatgtc tacgcacaaa taccactgcg tatgccctat gcgcagcgca gaagaccccg
361 aaaggctcga tagctacgca aagaaactgg cagcggcctc cgggaaggtg ctggatagag
421 agatcgcagg aaaaatcacc gacctgcaga ccgtcatggc tacgccagac gctgaatctc
481 ctaccttttg cctgcataca gacgtcacgt gtcgtacggc agccgaagtg gccgtatacc
541 aggacgtgta tgctgtacat gcaccaacat cgctgtacca tcaggcgatg aaaggtgtca
601 gaacggcgta ttggattggg tttgacacca ccccgtttat gtttgacgcg ctagcaggcg
661 cgtatccaac ctacgccaca aactgggccg acgagcaggt gttacaggcc aggaacatag
721 gactgtgtgc agcatccttg actgagggaa gactcggcaa actgtccatt ctccgcaaga
781 agcaattgaa accttgcgac acagtcatgt tctcggtagg atctacattg tacactgaga
841 gcagaaagct actgaggagc tggcacttac cctccgtatt ccacctgaaa ggtaaacaat
901 cctttacctg taggtgcgat accatcgtat catgtgaagg gtacgtagtt aagaaaatca
961 ctatgtgccc cggcctgtac ggtaaaacgg tagggtacgc cgtgacgtat cacgcggagg
1021 gattcctagt gtgcaagacc acagacactg tcaaaggaga aagagtctca ttccctgtat
1081 gcacctacgt cccctcaacc atctgtgatc aaatgactgg catactagcg accgacgtca
1141 caccggagga cgcacagaag ttgttagtgg gattgaatca gaggatagtt gtgaacggaa
1201 gaacacagcg aaacactaac acgatgaaga actatctgct tccgattgtg gccgtcgcat
1261 ttagcaagtg ggcgagggaa tacaaggcag accttgatga tgaaaaacct ctgggtgtcc
1321 gagagaggtc acttacttgc tgctgcttgt gggcatttaa aacgaggaag atgcacacca
1381 tgtacaagaa accagacacc cagacaatag tgaaggtgcc ttcagagttt aactcgttcg
1441 tcatcccgag cctatggtct acaggcctcg caatcccagt cagatcacgc attaagatgc
1501 ttttggccaa gaagaccaag cgagagttaa tacctgttct cgacgcgtcg tcagccaggg
1561 atgctgaaca agaggagaag gagaggttgg aggccgagct gactagagaa gccttaccac
1621 ccctcgtccc catcgcgccg gcggagacgg gagtcgtcga cgtcgacgtt gaagaactag
1681 agtatcacgc aggtgcaggg gtcgtggaaa cacctcgcag cgcgttgaaa gtcaccgcac
1741 agccgaacga cgtactacta ggaaattacg tagttctgtc cccgcagacc gtgctcaaga
1801 gctccaagtt ggcccccgtg caccctctag cagagcaggt gaaaataata acacataacg
1861 ggagggccgg cggttaccag gtcgacggat atgacggcag ggtcctacta ccatgtggat
1921 cggccattcc ggtccctgag tttcaagctt tgagcgagag cgccactatg gtgtacaacg
1981 aaagggagtt cgtcaacagg aaactatacc atattgccgt tcacggaccg tcgctgaaca
2041 ccgacgagga gaactacgag aaagtcagag ctgaaagaac tgacgccgag tacgtgttcg
2101 acgtagataa aaaatgctgc gtcaagagag aggaagcgtc gggtttggtg ttggtgggag
2161 agctaaccaa ccccccgttc catgaattcg cctacgaagg gctgaagatc aggccgtcgg
2221 caccatataa gactacagta gtaggagtct ttggggttcc gggatcaggc aagtctgcta
2281 ttattaagag cctcgtgacc aaacacgatc tggtcaccag cggcaagaag gagaactgcc
2341 aggaaatagt taacgacgtg aagaagcacc gcgggaaggg gacaagtagg gaaaacagtg
2401 actccatcct gctaaacggg tgtcgtcgtg ccgtggacat cctatatgtg gacgaggctt
2461 tcgcttgcca ttccggtact ctgctggccc taattgctct tgttaaacct cggagcaaag
2521 tggtgttatg cggagacccc aagcaatgcg gattcttcaa tatgatgcag cttaaggtga
2581 acttcaacca caacatctgc actgaagtat gtcataaaag tatatccaga cgttgcacgc
2641 gtccagtcac ggccatcgtg tctacgttgc actacggagg caagatgcgc acgaccaacc
2701 cgtgcaacaa acccataatc atagacacca caggacagac caagcccaag ccaggagaca
2761 tcgtgttaac atgcttccga ggctgggcaa agcagctgca gttggactac cgtggacacg
2821 aagtcatgac agcagcagca tctcagggcc tcacccgcaa aggggtatac gccgtaaggc
2881 agaaggtgaa tgaaaatccc ttgtatgccc ctgcgtcgga gcacgtgaat gtactgctga
2941 cgcgcactga ggataggctg gtgtggaaaa cgctggccgg cgatccctgg attaaggtcc
3001 tatcaaacat tccacagggt aactttacgg ccacattgga agaatggcaa gaagaacacg
3061 acaaaataat gaaggtgatt gaaggaccgg ctgcgcctgt ggacgcgttc cagaacaaag
3121 cgaacgtgtg ttgggcgaaa agcctggtgc ctgtcctgga cactgccgga atcagattga
3181 cagcagagga gtggagcacc ataattacag catttaagga ggacagagct tactctccag
3241 tggtggcctt gaatgaaatt tgcaccaagt actatggagt tgacctggac agtggcctgt
3301 tttctgcccc gaaggtgtcc ctgtattacg agaacaacca ctgggataac agacctggtg
3361 gaaggatgta tggattcaat gccgcaacag ctgccaggct ggaagctaga cataccttcc
3421 tgaaggggca gtggcatacg ggcaagcagg cagttatcgc agaaagaaaa atccaaccgc
3481 tttctgtgct ggacaatgta attcctatca accgcaggct gccgcacgcc ctggtggctg
3541 agtacaagac ggttaaaggc agtagggttg agtggctggt caataaagta agagggtacc
3601 acgtcctgct ggtgagtgag tacaacctgg ctttgcctcg acgcagggtc acttggttgt
3661 caccgctgaa tgtcacaggc gccgataggt gctacgacct aagtttagga ctgccggctg
3721 acgccggcag gttcgacttg gtctttgtga acattcacac ggaattcaga atccaccact
3781 accagcagtg tgtcgaccac gccatgaagc tgcagatgct tgggggagat gcgctacgac
3841 tgctaaaacc cggcggcatc ttgatgagag cttacggata cgccgataaa atcagcgaag
3901 ccgttgtttc ctccttaagc agaaagttct cgtctgcaag agtgttgcgc ccggattgtg
3961 tcaccagcaa tacagaagtg ttcttgctgt tctccaactt tgacaacgga aagagaccct
4021 ctacgctaca ccagatgaat accaagctga gtgccgtgta tgccggagaa gccatgcaca
4081 cggccgggtg tgcaccatcc tacagagtta agagagcaga catagccacg tgcacagaag
4141 cggctgtggt taacgcagct aacgcccgtg gaactgtagg ggatggcgta tgcagggccg
4201 tggcgaagaa atggccgtca gcctttaagg gagcagcaac accagtgggc acaattaaaa
4261 cagtcatgtg cggctcgtac cccgtcatcc acgctgtagc gcctaatttc tctgccacga
4321 ctgaagcgga aggggaccgc gaattggccg ctgtctaccg ggcagtggcc gccgaagtaa
4381 acagactgtc actgagcagc gtagccatcc cgctgctgtc cacaggagtg ttcagcggcg
4441 gaagagatag gctgcagcaa tccctcaacc atctattcac agcaatggac gccacggacg
4501 ctgacgtgac catctactgc agagacaaaa gttgggagaa gaaaatccag gaagccattg
4561 acatgaggac ggctgtggag ttgctcaatg atgacgtgga gctgaccaca gacttggtga
4621 gagtgcaccc ggacagcagc ctggtgggtc gtaagggcta cagtaccact gacgggtcgc
4681 tgtactcgta ctttgaaggt acgaaattca accaggctgc tattgatatg gcagagatac
4741 tgacgttgtg gcccagactg caagaggcaa acgaacagat atgcctatac gcgctgggcg
4801 aaacaatgga caacatcaga tccaaatgtc cggtgaacga ttccgattca tcaacacctc
4861 ccaggacagt gccctgcctg tgccgctacg caatgacagc agaacggatc gcccgcctta
4921 ggtcacacca agttaaaagc atggtggttt gctcatcttt tcccctcccg aaataccatg
4981 tagatggggt gcagaaggta aagtgcgaga aggttctcct gttcgacccg acggtacctt
5041 cagtggttag tccgcggaag tatgccgcat ctacgacgga ccactcagat cggtcgttac
5101 gagggtttga cttggactgg accaccgact cgtcttccac tgccagcgat accatgtcgc
5161 tacccagttt gcagtcgtgt gacatcgact cgatctacga gccaatggct cccatagtag
5221 tgacggctga cgtacaccct gaacccgcag gcatcgcgga cctggcggca gatgtgcacc
5281 ctgaacccgc agaccatgtg gacctcgaga acccgattcc tccaccgcgc ccgaagagag
5341 ctgcatacct tgcctcccgc gcggcggagc gaccggtgcc ggcgccgaga aagccgacgc
5401 ctgccccaag gactgcgttt aggaacaagc tgcctttgac gttcggcgac tttgacgagc
5461 acgaggtcga tgcgttggcc tccgggatta ctttcggaga cttcgacgac gtcctgcgac
5521 taggccgcgc gggtgcatat attttctcct cggacactgg cagcggacat ttacaacaaa
5581 aatccgttag gcagcacaat ctccagtgcg cacaactgga tgcggtccag gaggagaaaa
5641 tgtacccgcc aaaattggat actgagaggg agaagctgtt gctgctgaaa atgcagatgc
5701 acccatcgga ggctaataag agtcgatacc agtctcgcaa agtggagaac atgaaagcca
5761 cggtggtgga caggctcaca tcgggggcca gattgtacac gggagcggac gtaggccgca
5821 taccaacata cgcggttcgg tacccccgcc ccgtgtactc ccctaccgtg atcgaaagat
5881 tctcaagccc cgatgtagca atcgcagcgt gcaacgaata cctatccaga aattacccaa
5941 cagtggcgtc gtaccagata acagatgaat acgacgcata cttggacatg gttgacgggt
6001 cggatagttg cttggacaga gcgacattct gcccggcgaa gctccggtgc tacccgaaac
6061 atcatgcgta ccaccagccg actgtacgca gtgccgtccc gtcacccttt cagaacacac
6121 tacagaacgt gctagcggcc gccaccaaga gaaactgcaa cgtcacgcaa atgcgagaac
6181 tacccaccat ggactcggca gtgttcaacg tggagtgctt caagcgctat gcctgctccg
6241 gagaatattg ggaagaatat gctaaacaac ctatccggat aaccactgag aacatcacta
6301 cctatgtgac caaattgaaa ggcccgaaag ctgctgcctt gttcgctaag acccacaact
6361 tggttccgct gcaggaggtt cccatggaca gattcacggt cgacatgaaa cgagatgtca
6421 aagtcactcc agggacgaaa cacacagagg aaagacccaa agtccaggta attcaagcag
6481 cggagccatt ggcgaccgct tacctgtgcg gcatccacag ggaattagta aggagactaa
6541 atgctgtgtt acgccctaac gtgcacacat tgtttgatat gtcggccgaa gactttgacg
6601 cgatcatcgc ctctcacttc cacccaggag acccggttct agagacggac attgcatcat
6661 tcgacaaaag ccaggacgac tccttggctc ttacaggttt aatgatcctc gaagatctag
6721 gggtggatca gtacctgctg gacttgatcg aggcagcctt tggggaaata tccagctgtc
6781 acctaccaac tggcacgcgc ttcaagttcg gagctatgat gaaatcgggc atgtttctga
6841 ctttgtttat taacactgtt ttgaacatca ccatagcaag cagggtactg gagcagagac
6901 tcactgactc cgcctgtgcg gccttcatcg gcgacgacaa catcgttcac ggagtgatct
6961 ccgacaagct gatggcggag aggtgcgcgt cgtgggtcaa catggaggtg aagatcattg
7021 acgctgtcat gggcgaaaaa cccccatatt tttgtggggg attcatagtt tttgacagcg
7081 tcacacagac cgcctgccgt gtttcagacc cacttaagcg cctgttcaag ttgggtaagc
7141 cgctaacagc tgaagacaag caggacgaag acaggcgacg agcactgagt gacgaggtta
7201 gcaagtggtt ccggacaggc ttgggggccg aactggaggt ggcactaaca tctaggtatg
7261 aggtagaggg ctgcaaaagt atcctcatag ccatggccac cttggcgagg gacattaagg
7321 cgtttaagaa attgagagga cctgttatac acctctacgg cggtcctaga ttggtgcgtt
7381 aatacacaga attctgatta tagcgcacta ttatagcacc atggatcccg tcgttttaca
7441 acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc
7501 tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg
7561 cagcctgaat ggcgaatggc gctttgcctg gtttccggca ccagaagcgg tgccggaaag
7621 ctggctggag tgcgatcttc ctgaggccga tactgtcgtc gtcccctcaa actggcagat
7681 gcacggttac gatgcgccca tctacaccaa cgtaacctat cccattacgg tcaatccgcc
7741 gtttgttccc acggagaatc cgacgggttg ttactcgctc acatttaatg ttgatgaaag
7801 ctggctacag gaaggccaga cgcgaattat ttttgatggc gttaactcgg cgtttcatct
7861 gtggtgcaac gggcgctggg tcggttacgg ccaggacagt cgtttgccgt ctgaatttga
7921 cctgagcgca tttttacgcg ccggagaaaa ccgcctcgcg gtgatggtgc tgcgttggag
7981 tgacggcagt tatctggaag atcaggatat gtggcggatg agcggcattt tccgtgacgt
8041 ctcgttgctg cataaaccga ctacacaaat cagcgatttc catgttgcca ctcgctttaa
8101 tgatgatttc agccgcgctg tactggaggc tgaagttcag atgtgcggcg agttgcgtga
8161 ctacctacgg gtaacagttt ctttatggca gggtgaaacg caggtcgcca gcggcaccgc
8221 gcctttcggc ggtgaaatta tcgatgagcg tggtggttat gccgatcgcg tcacactacg
8281 tctgaacgtc gaaaacccga aactgtggag cgccgaaatc ccgaatctct atcgtgcggt
8341 ggttgaactg cacaccgccg acggcacgct gattgaagca gaagcctgcg atgtcggttt
8401 ccgcgaggtg cggattgaaa atggtctgct gctgctgaac ggcaagccgt tgctgattcg
8461 aggcgttaac cgtcacgagc atcatcctct gcatggtcag gtcatggatg agcagacgat
8521 ggtgcaggat atcctgctga tgaagcagaa caactttaac gccgtgcgct gttcgcatta
8581 tccgaaccat ccgctgtggt acacgctgtg cgaccgctac ggcctgtatg tggtggatga
8641 agccaatatt gaaacccacg gcatggtgcc aatgaatcgt ctgaccgatg atccgcgctg
8701 gctaccggcg atgagcgaac gcgtaacgcg aatggtgcag cgcgatcgta atcacccgag
8761 tgtgatcatc tggtcgctgg ggaatgaatc aggccacggc gctaatcacg acgcgctgta
8821 tcgctggatc aaatctgtcg atccttcccg cccggtgcag tatgaaggcg gcggagccga
8881 caccacggcc accgatatta tttgcccgat gtacgcgcgc gtggatgaag accagccctt
8941 cccggctgtg ccgaaatggt ccatcaaaaa atggctttcg ctacctggag agacgcgccc
9001 gctgatcctt tgcgaatacg cccacgcgat gggtaacagt cttggcggtt tcgctaaata
9061 ctggcaggcg tttcgtcagt atccccgttt acagggcggc ttcgtctggg actgggtgga
9121 tcagtcgctg attaaatatg atgaaaacgg caacccgtgg tcggcttacg gcggtgattt
9181 tggcgatacg ccgaacgatc gccagttctg tatgaacggt ctggtctttg ccgaccgcac
9241 gccgcatcca gcgctgacgg aagcaaaaca ccagcagcag tttttccagt tccgtttatc
9301 cgggcaaacc atcgaagtga ccagcgaata cctgttccgt catagcgata acgagctcct
9361 gcactggatg gtggcgctgg atggtaagcc gctggcaagc ggtgaagtgc ctctggatgt
9421 cgctccacaa ggtaaacagt tgattgaact gcctgaacta ccgcagccgg agagcgccgg
9481 gcaactctgg ctcacagtac gcgtagtgca accgaacgcg accgcatggt cagaagccgg
9541 gcacatcagc gcctggcagc agtggcgtct ggcggaaaac ctcagtgtga cgctccccgc
9601 cgcgtcccac gccatcccgc atctgaccac cagcgaaatg gatttttgca tcgagctggg
9661 taataagcgt tggcaattta accgccagtc aggctttctt tcacagatgt ggattggcga
9721 taaaaaacaa ctgctgacgc cgctgcgcga tcagttcacc cgtgcaccgc tggataacga
9781 cattggcgta agtgaagcga cccgcattga ccctaacgcc tgggtcgaac gctggaaggc
9841 ggcgggccat taccaggccg aagcagcgtt gttgcagtgc acggcagata cacttgctga
9901 tgcggtgctg attacgaccg ctcacgcgtg gcagcatcag gggaaaacct tatttatcag
9961 ccggaaaacc taccggattg atggtagtgg tcaaatggcg attaccgttg atgttgaagt
10021 ggcgagcgat acaccgcatc cggcgcggat tggcctgaac tgccagctgg cgcaggtagc
10081 agagcgggta aactggctcg gattagggcc gcaagaaaac tatcccgacc gccttactgc
10141 cgcctgtttt gaccgctggg atctgccatt gtcagacatg tataccccgt acgtcttccc
10201 gagcgaaaac ggtctgcgct gcgggacgcg cgaattgaat tatggcccac accagtggcg
10261 cggcgacttc cagttcaaca tcagccgcta cagtcaacag caactgatgg aaaccagcca
10321 tcgccatctg ctgcacgcgg aagaaggcac atggctgaat atcgacggtt tccatatggg
10381 gattggtggc gacgactcct ggagcccgtc agtatcggcg gaattccagc tgagcgccgg
10441 tcgctaccat taccagttgg tctggtgtca aaaataataa taaccgggca ggggggatcc
10501 cgggtaatta attgacgttt tacggccgcc ggtggcgccc gcgcccggcg gcccgtcctt
10561 ggccgttgca ggccactccg gtggctcccg tcgtccccga cttccaggcc cagcagatgc
10621 agcaactcat cagcgccgta aatgcgctga caatgagaca gaacgcaatt gctcctgcta
10681 ggcctcccaa accaaagaag aagaagacaa ccaaaccaaa gccgaaaacg cagcccaaga
10741 agatcaacgg aaaaacgcag cagcaaaaga agaaagacaa gcaagccgac aagaagaaga
10801 agaaacccgg aaaaagagaa agaatgtgca tgaagattga aaatgactgt atcttcgtat
10861 gcggctagcc acagtaacgt agtgtttcca gacatgtcgg gcaccgcact atcatgggtg
10921 cagaaaatct cgggtggtct gggggccttc gcaatcggcg ctatcctggt gctggttgtg
10981 gtcacttgca ttgggctccg cagataagtt agggtaggca atggcattga tatagcaaga
11041 aaattgaaaa cagaaaaagt tagggtaagc aatggcatat aaccataact gtataacttg
11101 taacaaagcg caacaagacc tgcgcaattg gccccgtggt ccgcctcacg gaaactcggg
11161 gcaactcata ttgacacatt aattggcaat aattggaagc ttacataagc ttaattcgac
11221 gaataattgg atttttattt tattttgcaa ttggttttta atatttccaa aaaaaaaaaa
11281 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaact
11341 agtctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
11401 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
11461 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
11521 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
11581 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
11641 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
11701 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
11761 gtggcgcttt ctcaatgctc gcgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
11821 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
11881 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
11941 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
12001 aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc
12061 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
12121 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
12181 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
12241 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
12301 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
12361 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
12421 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
12481 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
12541 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
12601 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
12661 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
12721 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
12781 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
12841 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
12901 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
12961 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
13021 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
13081 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
13141 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
13201 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
13261 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
13321 gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt
13381 atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg
13441 cagctcccgg agacggtcac agcttctgtc taagcggatg ccgggagcag acaagcccgt
13501 cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc ttaactatgc ggcatcagag
13561 cagattgtac tgagagtgca ccatatcgac gctctccctt atgcgactcc tgcattagga
13621 agcagcccag tactaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca
13681 aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac
13741 aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat
13801 aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga
13861 ggatctggct agcgatgacc ctgctgattg gttcgctgac catttccggg gtgcggaacg
13921 gcgttaccag aaactcagaa ggttcgtcca accaaaccga ctctgacggc agtttacgag
13981 agagatgata gggtctgctt cagtaagcca gatgctacac aattaggctt gtacatattg
14041 tcgttagaac gcggctacaa ttaatacata accttatgta tcatacacat acgatttagg
14101 tgacactata
//
