| 质粒名称: | pTRKH3-slpGFP |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 27168 |
| 存储实验室: | Michela Lizier |
|
载体骨架基本信息 |
|
| 载体名称: | pTRKH3 |
| 载体出品公司: | -- |
| 空载体大小: | 7493 bp |
| 完整质粒大小: | 8560bp |
| 载体修饰: | -- |
| 载体类型: | 细菌表达 |
| 筛选标记: | -- |
| 载体抗性: | 红霉素，200μg/mL |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 低拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | 表面（S）层蛋白启动子区与修饰的绿色荧光蛋白5融合 |
| 物种来源: | 乳酸乳球菌及其合成构建体 |
| 插入大小: | 1065 bp |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子: | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | SalI (未破坏) |
| 3’克隆位点: | BamHI (未破坏) |
| 5’测序引物 | AGTGAAAAGTTCTTCTCCTT |
| 3’测序引物 | GCGGCACGACTTCTTCAAGAGC |
|
文献引用方法 |
|
| 材料和方法部分: | pTRKH3-slpGFP was a gift from Michela Lizier (Addgene plasmid # 27168 ; http://n2t.net/addgene:27168 ; RRID:Addgene_27168) |
| 参考文献部分: | Comparison of expression vectors in Lactobacillus reuteri strains. Lizier M, Sarra PG, Cauda R, Lucchini F. FEMS Microbiol Lett. 2010 Jul 1. 308(1):8-15. 10.1111/j.1574-6968.2010.01978.x PubMed 20455948 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pTRKH3-slpGFP 8560 bp ds-DNA circular SYN 21-JAN-2022
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pTRKH3-slpGFP
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8560)
AUTHORS Lizier M, Sarra PG, Cauda R, Lucchini F
TITLE Comparison of expression vectors in Lactobacillus reuteri strains.
JOURNAL FEMS Microbiol Lett. 2010 Jul 1. 308(1):8-15.
PUBMED 20455948
REFERENCE 2 (bases 1 to 8560)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Jan 21, 2022 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..8560
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind 5753..5772
/label=CAT-R
/note="Chloramphenicol resistance gene, reverse primer"
promoter complement(5839..5941)
/label=cat promoter
/note="promoter of the E. coli cat gene encoding
chloramphenicol acetyltransferase"
primer_bind complement(6333..6350)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(6467..7012)
/direction=LEFT
/label=p15A ori
/note="Plasmids containing the medium-copy-number p15A
origin of replication can be propagated in E. coli cells
that contain a second plasmid with the ColE1 origin.
"
primer_bind complement(6547..6566)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
promoter 7124..7152
/label=tet promoter
/note="E. coli promoter for tetracycline efflux protein
gene"
CDS complement(7513..8229)
/codon_start=1
/gene="mgfp5"
/product="GFP with folding enhancement mutations"
/label=mgfp5
/note="suitable for expression in plants due to removal of
a cryptic intron"
/translation="MSKGEELFTGVVPILVELDGDVNGYKFSVSGEGEGDATYGKLTLK
FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDG
NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKA
NFKTRHNIEDGGVQLADHYQQNTPIGDDPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
FVTAAGITHGMDELYK"
primer_bind complement(7556..7576)
/label=GFP-F
/note="GFP, forward primer. Does NOT anneal to EGFP"
primer_bind 8171..8199
/label=GFP-R
/note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
1 atgcccttga gagccttcaa cccagtcagc tccttccggt gggcgcgggg catgactatc
61 gtcgccgcac ttatgactgt cttctttatc atgcaactcg taggacaggt gccggcagcg
121 ctctgggtca ttttcggcga ggaccgcttt cgctggagcg cgacgatgat cggcctgtcg
181 cttgcggtat tcggaatctt gcacgccctc gctcaagcct tcgtcactgg tcccgccacc
241 aaacgtttcg gcgagaagca ggccattatc gccggcatgg cggccgacgc gctgggctac
301 gtcttgctgg cgttcgcgac gcgaggctgg atggccttcc ccattatgat tcttctcgct
361 tccggcggca tcgggatgcc cgcgttgcag gccatgctgt ccaggcaggt agatgacgac
421 catcagggac agcttcaagg atcgctcgcg gctcttacca gcctaacttc gatcattgga
481 ccgctgatcg tcacggcgat ttatgccgcc tcggcgagca catggaacgg gttggcatgg
541 attgtaggcg ccgccctata ccttgtctgc ctccccgcgt tgcgtcgcgg tgcatggagc
601 cgggccacct cgacctgaat ggaagccggc ggcacctcgc taacggattc accactccaa
661 gaattggagc caatcaattc ttgcggagaa ctgtgaatgc gcaaaccaac ccttggcaga
721 acatatccat cgcgtccgcc atctccagca gccgcacgcg gcgcatctcg gtcgagtcta
781 gaatcgatac gattttgaag tggcaacaga taaaaaaaag cagtttaaaa ttgttgctga
841 acttttaaaa caagcaaata caatcattgt cgcaacagat agcgacagag aaggcgaaaa
901 cattgcctgg tcgatcattc ataaagcaaa tgccttttct aaagataaaa cgtataaaag
961 actatggatc aatagtttag aaaaagatgt gatccgtagc ggttttcaaa atttgcaacc
1021 aggaatgaat tactatccct tttatcaaga agcgcacaaa aagaaaaacg aaatgataca
1081 ccaatcagtg caaaaaaaga tataatggga gataagacgg ttcgtgttcg tgctgacttg
1141 caccatatca taaaaatcga aacagcaaag aatggcggaa acgtaaaaga agttatggaa
1201 ataagactta gaagcaaact taagagtgtg ttgatagtgc agtatcttaa aattttgtat
1261 aataggaatt gaagttaaat tagatgctaa aaatttgtaa ttaagaagga gtgattacat
1321 gaacaaaaat ataaaatatt ctcaaaactt tttaacgagt gaaaaagtac tcaaccaaat
1381 aataaaacaa ttgaatttaa aagaaaccga taccgtttac gaaattggaa caggtaaagg
1441 gcatttaacg acgaaactgg ctaaaataag taaacaggta acgtctattg aattagacag
1501 tcatctattc aacttatcgt cagaaaaatt aaaactgaat actcgtgtca ctttaattca
1561 ccaagatatt ctacagtttc aattccctaa caaacagagg tataaaattg ttgggagtat
1621 tccttaccat ttaagcacac aaattattaa aaaagtggtt tttgaaagcc atgcgtctga
1681 catctatctg attgttgaag aaggattcta caagcgtacc ttggatattc accgaacact
1741 agggttgctc ttgcacactc aagtctcgat tcagcaattg cttaagctgc cagcggaatg
1801 ctttcatcct aaaccaaaag taaacagtgt cttaataaaa cttacccgcc ataccacaga
1861 tgttccagat aaatattgga agctatatac gtactttgtt tcaaaatggg tcaatcgaga
1921 atatcgtcaa ctgtttacta aaaatcagtt tcatcaagca atgaaacacg ccaaagtaaa
1981 caatttaagt accgttactt atgagcaagt attgtctatt tttaatagtt atctattatt
2041 taacgggagg aaataattct atgagtcgct tttgtaaatt tggaaagtta cacgttacta
2101 aagggaatgt agataaatta ttaggtatac tactgacagc ttccaaggag ctaaagaggt
2161 ccctagcgct cttatcatgg ggaagctcgg atcatatgca agacaaaata aactcgcaac
2221 agcacttgga gaaatgggac gaatcgagaa aaccctcttt acgctggatt acatatctaa
2281 taaagccgta aggagacggg ttcaaaaagg tttaaataaa ggagaagcaa tcaatgcatt
2341 agctagaatt atattttttg gacaacgtgg agaatttaga gaacgtgctc tccaagacca
2401 gttacaaaga gctagtgcac taaacataat tattaacgct ataagtgtgt ggaacactgt
2461 atatatggaa aaagccgtag aagaattaaa agcaagagga gaatttagag aagatttaat
2521 gccatatgcg tggccgttag gatgggaaca tatcaatttt cttggagaat acaaatttga
2581 aggattacat gacactgggc aaatgaattt acgtccttta cgtataaaag agccgtttta
2641 ttcttaatat aacggctctt tttatagaaa aaatccttag cgtggttttt ttccgaaatg
2701 ctggcggtac cccaagaatt agaaatgagt agatcaaatt attcacgaat agaatcagga
2761 aaatcagatc caaccataaa aacactagaa caaattgcaa agttaactaa ctcaacgcta
2821 gtagtggatt taatcccaaa tgagccaaca gaaccagaac cagaaacaga atcagaacaa
2881 gtaacattgg atttagaaat ggaagaagaa aaaagcaatg acttcgtgtg aataatgcac
2941 gaaatcgttg cttatttttt ttaaaagcgg tatactagat ataacgaaac aacgaactga
3001 atagaaacga aaaaagagcc atgacacatt tataaaatgt ttgacgacat tttataaatg
3061 catagcccga taagattgcc aaaccaacgc ttatcagtta gtcagatgaa ctcttccctc
3121 gtaagaagtt atttaattaa ctttgtttga agacggtata taaccgtact atcattatat
3181 agggaaatca gagagttttc aagtatctaa gctactgaat ttaagaattg ttaagcaatc
3241 aatcggaaat cgtttgattg ctttttttgt attcatttat agaaggtgga gtttgtatga
3301 atcatgatga atgtaaaact tatataaaaa atagtttatt ggagataaga aaattagcaa
3361 atatctatac actagaaacg tttaagaaag agttagaaaa gagaaatatc tacttagaaa
3421 caaaatcaga taagtatttt tcttcggagg gggaagatta tatatataag ttaatagaaa
3481 ataacaaaat aatttattcg attagtggaa aaaaattgac ttataaagga aaaaaatctt
3541 tttcaaaaca tgcaatattg aaacagttga atgaaaaagc aaaccaagtt aattaaacaa
3601 cctattttat aggatttata ggaaaggaga acagctgaat gaatatccct tttgttgtag
3661 aaactgtgct tcatgacggc ttgttaaagt acaaatttaa aaatagtaaa attcgctcaa
3721 tcactaccaa gccaggtaaa agcaaagggg ctatttttgc gtatcgctca aaatcaagca
3781 tgattggcgg tcgtggtgtt gttctgactt ccgaggaagc gattcaagaa aatcaagata
3841 catttacaca ttggacaccc aacgtttatc gttatggaac gtatgcagac gaaaaccgtt
3901 catacacgaa aggacattct gaaaacaatt taagacaaat caataccttc tttattgatt
3961 ttgatattca cacggcaaaa gaaactattt cagcaagcga tattttaaca accgctattg
4021 atttaggttt tatgcctact atgattatca aatctgataa aggttatcaa gcatattttg
4081 ttttagaaac gccagtctat gtgacttcaa aatcagaatt taaatctgtc aaagcagcca
4141 aaataatttc gcaaaatatc cgagaatatt ttggaaagtc tttgccagtt gatctaacgt
4201 gtaatcattt tggtattgct cgcataccaa gaacggacaa tgtagaattt tttgatccta
4261 attaccgtta ttctttcaaa gaatggcaag attggtcttt caaacaaaca gataataagg
4321 gctttactcg ttcaagtcta acggttttaa gcggtacaga aggcaaaaaa caagtagatg
4381 aaccctggtt taatctctta ttgcacgaaa cgaaattttc aggagaaaag ggtttaatag
4441 ggcgtaataa cgtcatgttt accctctctt tagcctactt tagttcaggc tattcaatcg
4501 aaacgtgcga atataatatg tttgagttta ataatcgatt agatcaaccc ttagaagaaa
4561 aagaagtaat caaaattgtt agaagtgcct attcagaaaa ctatcaaggg gctaataggg
4621 aatacattac cattctttgc aaagcttggg tatcaagtga tttaaccagt aaagatttat
4681 ttgtccgtca agggtggttt aaattcaaga aaaaaagaag cgaacgtcaa cgtgttcatt
4741 tgtcagaatg gaaagaagat ttaatggctt atattagcga aaaaagcgat gtatacaagc
4801 cttatttagt gacgaccaaa aaagagatta gagaagtgct aggcattcct gaacggacat
4861 tagataaatt gctgaaggta ctgaaggcga atcaggaaat tttctttaag attaaaccag
4921 gaagaaatgg tggcattcaa cttgctagtg ttaaatcatt gttgctatcg atcattaaag
4981 taaaaaaaga agaaaaagaa agctatataa aggcgctgac aaattctttt gacttagagc
5041 atacattcat tcaagagact ttaaacaagc tagcagaacg ccctaaaacg gacacacaac
5101 tcgatttgtt tagctatgat acaggctgaa aataaaaccc gcactatgcc attacattta
5161 tatctatgat acgtgtttgt tttttctttg ctgtttagcg aatgattagc agaaatatac
5221 agagtaagat tttaattaat tattaggggg agaaggagag agtagcccga aaacttttag
5281 ttggcttgga ctgaacgaag tgagggaaag gctactaaaa cgtcgagggg cagtgagagc
5341 gaagcgaaca cttgattttt taattttcta tcttttatag gtcattagag tatacttatt
5401 tgtcctataa actatttagc agcataatag atttattgaa taggtcattt aagttgagca
5461 tattagagga ggaaaatctt ggagaaatat ttgaagaacc cgattacatg gattggatta
5521 gttcttgtgg ttacgtggtt tttaactaaa agtagtgaat ttttgatttt tggtgtgtgt
5581 gtcttgttgt tagtatttgc tagtcaaagt gattaaatag aattccggat gagcattcat
5641 caggcgggca agaatgtgaa taaaggccgg ataaaacttg tgcttatttt tctttacggt
5701 ctttaaaaag gccgtaatat ccagctgaac ggtctggtta taggtacatt gagcaactga
5761 ctgaaatgcc tcaaaatgtt ctttacgatg ccattgggat atatcaacgg tggtatatcc
5821 agtgattttt ttctccattt tagcttcctt agctcctgaa aatctcgata actcaaaaaa
5881 tacgcccggt agtgatctta tttcattatg gtgaaagttg gaacctctta cgtgccgatc
5941 aacgtctcat tttcgccaaa agttggccca gggcttcccg gtatcaacag ggacaccagg
6001 atttatttat tctgcgaagt gatcttccgt cacaggtatt tattcggcgc aaagtgcgtc
6061 gggtgatgct gccaacttac tgatttagtg tatgatggtg tttttgaggt gctccagtgg
6121 cttctgtttc tatcagctgt ccctcctgtt cagctactga cggggtggtg cgtaacggca
6181 aaagcaccgc cggacatcag cgctagcgga gtgtatactg gcttactatg ttggcactga
6241 tgagggtgtc agtgaagtgc ttcatgtggc aggagaaaaa aggctgcacc ggtgcgtcag
6301 cagaatatgt gatacaggat atattccgct tcctcgctca ctgactcgct acgctcggtc
6361 gttcgactgc ggcgagcgga aatggcttac gaacggggcg gagatttcct ggaagatgcc
6421 aggaagatac ttaacaggga agtgagaggg ccgcggcaaa gccgtttttc cataggctcc
6481 gcccccctga caagcatcac gaaatctgac gctcaaatca gtggtggcga aacccgacag
6541 gactataaag ataccaggcg tttccccctg gcggctccct cgtgcgctct cctgttcctg
6601 cctttcggtt taccggtgtc attccgctgt tatggccgcg tttgtctcat tccacgcctg
6661 acactcagtt ccgggtaggc agttcgctcc aagctggact gtatgcacga accccccgtt
6721 cagtccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggaaagacat
6781 gcaaaagcac cactggcagc agccactggt aattgattta gaggagttag tcttgaagtc
6841 atgcgccggt taaggctaaa ctgaaaggac aagttttggt gactgcgctc ctccaagcca
6901 gttacctcgg ttcaaagagt tggtagctca gagaaccttc gaaaaaccgc cctgcaaggc
6961 ggttttttcg ttttcagagc aagagattac gcgcagacca aaacgatctc aagaagatca
7021 tcttattaat cagataaaat atttctagat ttcagtgcaa tttatctctt caaatgtagc
7081 acctgaagtc agccccatac gatataagtt gtaattctca tgtttgacag cttatcatcg
7141 ataagcttta atgcggtagt ttatcacagt taaattgcta acgcagtcag gcaccgtgta
7201 tgaaatctaa caatgcgctc atcgtcatcc tcggcaccgt caccctggat gctgtaggca
7261 taggcttggt tatgccggta ctgccgggcc tcttgcggga tatcgtccat tccgacagca
7321 tcgccagtca ctatggcgtg ctgctagcgc tatatgcgtt gatgcaattt ctatgcgcac
7381 ccgttctcgg agcactgtcc gaccgctttg gccgccgccc agtcctgctc gcttcgctac
7441 ttggagccac tatcgactac gcgatcatgg cgaccacacc cgtcctgtgg atcccccggg
7501 ctgcaggtcg agttatttgt atagttcatc catgccatgt gtaatcccag cagccgttac
7561 aaactcaaga aggaccatgt ggtctctctt ttcgttggga tctttcgaaa gggcagattg
7621 tgtggacagg taatggttgt ctggtaaaag gacagggtca tcgccaattg gagtattttg
7681 ttgataatga tcagcgagtt gcacgccgcc gtcttcgatg ttgtggcggg tcttgaagtt
7741 ggctttgatg ccgttctttt gcttgtcggc catgatgtat acgttgtggg agttgtagtt
7801 gtattccaac ttgtggccga ggatgtttcc gtcctccttg aaatcgattc ccttaagctc
7861 gatcctgttg acgagggtgt ctccctcaaa cttgacttca gcacgtgtct tgtagttccc
7921 gtcgtccttg aagaagatgg tcctctcctg cacgtatccc tcaggcatgg cgctcttgaa
7981 gaagtcgtgc cgcttcatat gatctggata tcttgaaaag cattgaacac cataagtgag
8041 agtagtgaca agtgttggcc atggaacagg tagttttcca gtagtgcaaa taaatttaag
8101 ggtaagtttt ccgtatgttg catcaccttc accctctcca ctgacagaaa atttgtaccc
8161 attaacatca ccatctaatt caacaagaat tgggacaact ccagtgaaaa gttcttctcc
8221 tttactcatg gtggctgtgg atctgctaac agtagataca gcagaagcag caactggagc
8281 aacagcaagt aaagcagcag cagcagcgct aacgattctt aaatttttct tcatgtggtc
8341 ttttcctcct tgaaatataa aaaaatgtaa taggccatag gttcagcatg tgataggagc
8401 ctataatgta caaaaccctt caaagcaaga tccatgagtc tataactctc atgaggtagt
8461 agtcttgctg aagaatcagc atgcgtacag gcaacacgcc tgaagcacgt cctattactt
8521 accacgaatt cgatatcaag cttatcgata ccgtcgaccg
//
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