| 质粒名称: | pTarget_CAST |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 127926 |
| 存储实验室: | Feng Zhang |
|
载体骨架基本信息 |
|
| 载体名称: | pACYC |
| 载体出品公司: | -- |
| 空载体大小: | -- |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | -- |
| 筛选标记: | -- |
| 载体抗性: | 氯霉素,25 μg/mL |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 低拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | PSP1 target (GGTT PAM) |
| 物种来源: | -- |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子: | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | -- |
| 5’克隆位点: | -- |
| 3’克隆位点: | -- |
| 5’测序引物 | -- |
| 3’测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pTarget_CAST was a gift from Feng Zhang (Addgene plasmid # 127926 ; http://n2t.net/addgene:127926 ; RRID:Addgene_127926) |
| 参考文献部分: | RNA-guided DNA insertion with CRISPR-associated transposases. Strecker J, Ladha A, Gardner Z, Schmid-Burgk JL, Makarova KS, Koonin EV, Zhang F. Science. 2019 Jul 5;365(6448):48-53. doi: 10.1126/science.aax9181. Epub 2019 Jun 6. 10.1126/science.aax9181 PubMed 31171706 |
买家导航
pTargetCAST载体基本信息
pTargetCAST载体图谱质粒图谱和多克隆位点信息
pTargetCAST载体简介
pTargetCAST载体序列
LOCUS pTarget_CAST 2899 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Target plasmid containing PSP1.
ACCESSION .
VERSION .
KEYWORDS pTarget_CAST
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 2899)
AUTHORS Strecker J, Ladha A, Gardner Z, Schmid-Burgk JL, Makarova KS, Koonin
EV, Zhang F
TITLE RNA-guided DNA insertion with CRISPR-associated transposases.
JOURNAL Science. 2019 Jul 5;365(6448):48-53. doi: 10.1126/science.aax9181.
Epub 2019 Jun 6.
PUBMED 31171706
REFERENCE 2 (bases 1 to 2899)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..2899
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind complement(288..305)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(422..967)
/direction=LEFT
/label=p15A ori
/note="Plasmids containing the medium-copy-number p15A
origin of replication can be propagated in E. coli cells
that contain a second plasmid with the ColE1 origin.
"
primer_bind complement(502..521)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind complement(1583..1602)
/label=pBRrevBam
/note="pBR322 vectors, tet region, downstream of BamHI,
reverse primer"
CDS complement(2033..2692)
/codon_start=1
/gene="cat"
/product="chloramphenicol acetyltransferase"
/label=CmR
/note="confers resistance to chloramphenicol"
/translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
primer_bind 2607..2626
/label=CAT-R
/note="Chloramphenicol resistance gene, reverse primer"
promoter complement(2693..2795)
/label=cat promoter
/note="promoter of the E. coli cat gene encoding
chloramphenicol acetyltransferase"
ORIGIN
1 ggcgcaaagt gcgtcgggtg atgctgccaa cttactgatt tagtgtatga tggtgttttt
61 gaggtgctcc agtggcttct gtttctatca gctgtccctc ctgttcagct actgacgggg
121 tggtgcgtaa cggcaaaagc accgccggac atcagcgcta gcggagtgta tactggctta
181 ctatgttggc actgatgagg gtgtcagtga agtgcttcat gtggcaggag aaaaaaggct
241 gcaccggtgc gtcagcagaa tatgtgatac aggatatatt ccgcttcctc gctcactgac
301 tcgctacgct cggtcgttcg actgcggcga gcggaaatgg cttacgaacg gggcggagat
361 ttcctggaag atgccaggaa gatacttaac agggaagtga gagggccgcg gcaaagccgt
421 ttttccatag gctccgcccc cctgacaagc atcacgaaat ctgacgctca aatcagtggt
481 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggcggc tccctcgtgc
541 gctctcctgt tcctgccttt cggtttaccg gtgtcattcc gctgttatgg ccgcgtttgt
601 ctcattccac gcctgacact cagttccggg taggcagttc gctccaagct ggactgtatg
661 cacgaacccc ccgttcagtc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
721 aacccggaaa gacatgcaaa agcaccactg gcagcagcca ctggtaattg atttagagga
781 gttagtcttg aagtcatgcg ccggttaagg ctaaactgaa aggacaagtt ttggtgactg
841 cgctcctcca agccagttac ctcggttcaa agagttggta gctcagagaa ccttcgaaaa
901 accgccctgc aaggcggttt tttcgttttc agagcaagag attacgcgca gaccaaaacg
961 atctcaagaa gatcatctta ttaatatacg tagtatctac gatacgtagt atctacgata
1021 cgtaggaggt tgagaagtca tttaataagg ccactgttaa acgtagatat atctacgcgt
1081 agatatatct acgatacgta gcggccgacg cgctgggcta cgtcttaatg ggcagcgccc
1141 acatacgcag cgatttccaa cagcttgagg cggaagcttt aatgcggtag tttatcacag
1201 ttaaattgct aacgcagtca ggcaccgtgt atgaaatcta acaatgcgct catcgtcatc
1261 ctcggcaccg tcaccctgga tgctgtaggc ataggcttgg ttatgccggt actgccgggc
1321 ctcttgcggg atatcgtcca ttccgacagc atcgccagtc actatggcgt gctgctagcg
1381 ctatatgcgt tgatgcaatt tctatgcgca cccgttctcg gagcactgtc cgaccgcttt
1441 ggccgccgcc cagtcctgct cgcttcgcta cttggagcca ctatcgacta cgcgatcatg
1501 gcgaccacac ccgtcctgtg gatcctctac gccggacgca tcgtggccgg catcaccggc
1561 gccacaggtg cggttgctgg cgcctatatc gccgacatca ccgatgggga agatcgggcc
1621 tgccaccata cccacgccga aacaagcgcc ctgcaccatt atgttccgga tctgcatcgc
1681 aggatgctgc tggctaccct gtggaacacc tacatctgta ttaacgaagc gctaaccgtt
1741 tttatcaggc tctgggaggc agaataaatg atcatatcgt caattattac ctccacgggg
1801 agagcctgag caaactggcc tcaggcattt gagaagcaca cggtcacact gcttccggta
1861 gtcaataaac cggtaaacca gcaatagaca taagcggcta tttaacgacc ctgccctgaa
1921 ccgacgaccg ggtcgaattt gctttcgaat ttctgccatt catccgctta ttatcactta
1981 ttcaggcgta gcaaccaggc gtttaagggc accaataact gccttaaaaa aattacgccc
2041 cgccctgcca ctcatcgcag tactgttgta attcattaag cattctgccg acatggaagc
2101 catcacaaac ggcatgatga acctgaatcg ccagcggcat cagcaccttg tcgccttgcg
2161 tataatattt gcccatggtg aaaacggggg cgaagaagtt gtccatattg gccacgttta
2221 aatcaaaact ggtgaaactc acccagggat tggctgagac gaaaaacata ttctcaataa
2281 accctttagg gaaataggcc aggttttcac cgtaacacgc cacatcttgc gaatatatgt
2341 gtagaaactg ccggaaatcg tcgtggtatt cactccagag cgatgaaaac gtttcagttt
2401 gctcatggaa aacggtgtaa caagggtgaa cactatccca tatcaccagc tcaccgtctt
2461 tcattgccat acggaattcc ggatgagcat tcatcaggcg ggcaagaatg tgaataaagg
2521 ccggataaaa cttgtgctta tttttcttta cggtctttaa aaaggccgta atatccagct
2581 gaacggtctg gttataggta cattgagcaa ctgactgaaa tgcctcaaaa tgttctttac
2641 gatgccattg ggatatatca acggtggtat atccagtgat ttttttctcc attttagctt
2701 ccttagctcc tgaaaatctc gataactcaa aaaatacgcc cggtagtgat cttatttcat
2761 tatggtgaaa gttggaacct cttacgtgcc gatcaacgtc tcattttcgc caaaagttgg
2821 cccagggctt cccggtatca acagggacac caggatttat ttattctgcg aagtgatctt
2881 ccgtcacagg tatttattc
//
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