| 质粒名称: | pWPT-GFP |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 12255 |
| 存储实验室: | Didier Trono |
|
载体骨架基本信息 |
|
| 载体名称: | pWPT |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 8754 bp |
| 完整质粒大小: | -- |
| 载体修饰: | 人细胞色素c氧化酶亚基VIII（human cytochrome C oxidase subunit VIII） |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | 新霉素（G418） |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | EGFP |
| 物种来源: | -- |
| 插入大小: | 716bp |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | 人细胞色素c氧化酶亚基VIII（N端） |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | AgeI(未破坏) |
| 3' 克隆位点: | NotI (未破坏) |
| 5' 测序引物 | CMV-F |
| 3' 测序引物 | SV40pA-R |
|
文献引用方法 |
|
| 材料和方法部分: | pWPT-GFP was a gift from Didier Trono (Addgene plasmid # 12255 ; http://n2t.net/addgene:12255 ; RRID:Addgene_12255) |
| 参考文献部分: | -- |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pWPT-GFP 9470 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pWPT-GFP
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9470)
TITLE Trono Lab Constitutive Lentiviral Plasmids
REFERENCE 2 (bases 1 to 9470)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..9470
/organism="synthetic DNA construct"
/mol_type="other DNA"
LTR 2..635
/label=3' LTR
/note="3' long terminal repeat (LTR) from HIV-1"
misc_feature 682..807
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 1304..1537
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
misc_feature 2033..2150
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
promoter 2206..2417
/label=EF-1-alpha core promoter
/note="core promoter for human elongation factor
EF-1-alpha"
regulatory 2470..2479
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 2476..3192
/codon_start=1
/product="enhanced GFP"
/label=EGFP
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
primer_bind complement(2521..2542)
/label=EGFP-N
/note="EGFP, reverse primer"
primer_bind complement(2782..2801)
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
primer_bind 3129..3150
/label=EGFP-C
/note="EGFP, forward primer"
primer_bind complement(3270..3286)
/label=KS primer
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(3271..3287)
/label=pBluescriptKS
/note="For pBluescript vector"
misc_feature 3300..3888
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(3353..3373)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(3771..3782)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
protein_bind 4032..4065
/label=loxP
/bound_moiety="Cre recombinase"
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
LTR 4085..4265
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
promoter complement(4290..4308)
/label=SP6 promoter
/note="promoter for bacteriophage SP6 RNA polymerase"
primer_bind complement(4291..4308)
/label=SP6
/note="SP6 promoter, forward primer"
primer_bind complement(4598..4617)
/label=pBRrevBam
/note="pBR322 vectors, tet region, downstream of BamHI,
reverse primer"
primer_bind complement(4831..4850)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 4950..4972
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind complement(5010..5028)
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
promoter 5096..5200
/gene="bla"
/label=AmpR promoter
CDS 5201..6061
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(5419..5438)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 6232..6820
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 6721..6740
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 6974..6991
/label=L4440
/note="L4440 vector, forward primer"
primer_bind complement(7061..7081)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 7066..7395
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 7246..7381
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 7308..7327
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
intron 8529..8594
/label=small t intron
/note="SV40 (simian virus 40) small t antigen intron"
CDS 8724..8744
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
polyA_signal 9169..9303
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(9206..9225)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 9260..9279
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
ORIGIN
1 ttggaagggc taattcactc ccaaagaaga caagatatcc ttgatctgtg gatctaccac
61 acacaaggct acttccctga ttagcagaac tacacaccag ggccaggggt cagatatcca
121 ctgacctttg gatggtgcta caagctagta ccagttgagc cagataaggt agaagaggcc
181 aataaaggag agaacaccag cttgttacac cctgtgagcc tgcatgggat ggatgacccg
241 gagagagaag tgttagagtg gaggtttgac agccgcctag catttcatca cgtggcccga
301 gagctgcatc cggagtactt caagaactgc tgatatcgag cttgctacaa gggactttcc
361 gctggggact ttccagggag gcgtggcctg ggcgggactg gggagtggcg agccctcaga
421 tcctgcatat aagcagctgc tttttgcctg tactgggtct ctctggttag accagatctg
481 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
541 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
601 cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa
661 gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg
721 gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag
781 aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg
841 gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg
901 gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc
961 tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga
1021 tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac
1081 accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag
1141 caagcggccg gccgctgatc ttcagacctg gaggaggaga tatgagggac aattggagaa
1201 gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg
1261 caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct ttgttccttg
1321 ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg acggtacagg
1381 ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg gctattgagg
1441 cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag gcaagaatcc
1501 tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt tgctctggaa
1561 aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa tctctggaac
1621 agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat tacacaagct
1681 taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa caagaattat
1741 tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat tggctgtggt
1801 atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata gtttttgctg
1861 tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt cagacccacc
1921 tcccaacccc gaggggaccc gacaggcccg aaggaataga agaagaaggt ggagagagag
1981 acagagacag atccattcga ttagtgaacg gatctcgacg gtatcggtta acttttaaaa
2041 gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag
2101 acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt ccgatcacga
2161 gactagcctc gagaagcttg atcgatggct ccggtgcccg tcagtgggca gagcgcacat
2221 cgcccacagt ccccgagaag ttggggggag gggtcggcaa ttgaaccggt gcctagagaa
2281 ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg
2341 gtgggggaga accgtatata agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt
2401 ttgccgccag aacacaggtg tcgtgacgcg gatccaggcc taagcttacg cgtcctagcg
2461 ctaccggtcg ccaccatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc
2521 ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag
2581 ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc
2641 gtgccctggc ccaccctcgt gaccaccctg acctacggcg tgcagtgctt cagccgctac
2701 cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag
2761 gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc
2821 gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc
2881 aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcatggcc
2941 gacaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacat cgaggacggc
3001 agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg
3061 ctgcccgaca accactacct gagcacccag tccgccctga gcaaagaccc caacgagaag
3121 cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac
3181 gagctgtaca agtccggact cagatctcga ctagctagta gctagctagc tagtcgagct
3241 caacttcgaa ttcgatatca agcttatcgc gataccgtcg acctcgaggg aattccgata
3301 atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc
3361 cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta
3421 tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt
3481 ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg
3541 gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta
3601 ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt
3661 tgggcactga caattccgtg gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg
3721 cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca
3781 atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc
3841 gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcat cgggaattcg
3901 agctcggtac ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta
3961 aaagaaaagg ggggactgga agggctaatt cactcccaac gaagacaaga tgggatcaat
4021 tcaccatggg aataacttcg tatagcatac attatacgaa gttatgctgc tttttgcttg
4081 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
4141 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
4201 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
4261 tagcagcatc tagaattaat tccgtgtatt ctatagtgtc acctaaatcg tatgtgtatg
4321 atacataagg ttatgtatta attgtagccg cgttctaacg acaatatgta caagcctaat
4381 tgtgtagcat ctggcttact gaagcagacc ctatcatctc tctcgtaaac tgccgtcaga
4441 gtcggtttgg ttggacgaac cttctgagtt tctggtaacg ccgtcccgca cccggaaatg
4501 gtcagcgaac caatcagcag ggtcatcgct agccagatcc tctacgccgg acgcatcgtg
4561 gccggcatca ccggcgccac aggtgcggtt gctggcgcct atatcgccga catcaccgat
4621 ggggaagatc gggctcgcca cttcgggctc atgagcgctt gtttcggcgt gggtatggtg
4681 gcaggccccg tggccggggg actgttgggc gccatctcct tgcatgcacc attccttgcg
4741 gcggcggtgc tcaacggcct caacctacta ctgggctgct tcctaatgca ggagtcgcat
4801 aagggagagc gtcgaatggt gcactctcag tacaatctgc tctgatgccg catagttaag
4861 ccagccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc
4921 atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc
4981 gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt tataggttaa
5041 tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg
5101 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
5161 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
5221 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
5281 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
5341 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
5401 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga
5461 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
5521 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
5581 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
5641 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
5701 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
5761 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
5821 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
5881 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
5941 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
6001 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
6061 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
6121 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
6181 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
6241 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
6301 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
6361 gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc
6421 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
6481 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
6541 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
6601 actgagatac ctacagcgtg agcattgaga aagcgccacg cttcccgaag ggagaaaggc
6661 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
6721 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
6781 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
6841 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
6901 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg
6961 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc
7021 gcctctcccc gcgcgttggc cgattcatta atgcagctgt ggaatgtgtg tcagttaggg
7081 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
7141 tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
7201 catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact
7261 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
7321 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
7381 ctaggctttt gcaaaaagct tggacacaag acaggcttgc gagatatgtt tgagaatacc
7441 actttatccc gcgtcaggga gaggcagtgc gtaaaaagac gcggactcat gtgaaatact
7501 ggtttttagt gcgccagatc tctataatct cgcgcaacct attttcccct cgaacacttt
7561 ttaagccgta gataaacagg ctgggacact tcacatgagc gaaaaataca tcgtcacctg
7621 ggacatgttg cagatccatg cacgtaaact cgcaagccga ctgatgcctt ctgaacaatg
7681 gaaaggcatt attgccgtaa gccgtggcgg tctgtaccgg gtgcgttact ggcgcgtgaa
7741 ctgggtattc gtcatgtcga taccgtttgt atttccagct acgatcacga caaccagcgc
7801 gagcttaaag tgctgaaacg cgcagaaggc gatggcgaag gcttcatcgt tattgatgac
7861 ctggtggata ccggtggtac tgcggttgcg attcgtgaaa tgtatccaaa agcgcacttt
7921 gtcaccatct tcgcaaaacc ggctggtcgt ccgctggttg atgactatgt tgttgatatc
7981 ccgcaagata cctggattga acagccgtgg gatatgggcg tcgtattcgt cccgccaatc
8041 tccggtcgct aatcttttca acgcctggca ctgccgggcg ttgttctttt taacttcagg
8101 cgggttacaa tagtttccag taagtattct ggaggctgca tccatgacac aggcaaacct
8161 gagcgaaacc ctgttcaaac cccgctttaa acatcctgaa acctcgacgc tagtccgccg
8221 ctttaatcac ggcgcacaac cgcctgtgca gtcggccctt gatggtaaaa ccatccctca
8281 ctggtatcgc atgattaacc gtctgatgtg gatctggcgc ggcattgacc cacgcgaaat
8341 cctcgacgtc caggcacgta ttgtgatgag cgatgccgaa cgtaccgacg atgatttata
8401 cgatacggtg attggctacc gtggcggcaa ctggatttat gagtgggccc cggatctttg
8461 tgaaggaacc ttacttctgt ggtgtgacat aattggacaa actacctaca gagatttaaa
8521 gctctaaggt aaatataaaa tttttaagtg tataatgtgt taaactactg attctaattg
8581 tttgtgtatt ttagattcca acctatggaa ctgatgaatg ggagcagtgg tggaatgcct
8641 ttaatgagga aaacctgttt tgctcagaag aaatgccatc tagtgatgat gaggctactg
8701 ctgactctca acattctact cctccaaaaa agaagagaaa ggtagaagac cccaaggact
8761 ttccttcaga attgctaagt tttttgagtc atgctgtgtt tagtaataga actcttgctt
8821 gctttgctat ttacaccaca aaggaaaaag ctgcactgct atacaagaaa attatggaaa
8881 aatattctgt aacctttata agtaggcata acagttataa tcataacata ctgttttttc
8941 ttactccaca caggcataga gtgtctgcta ttaataacta tgctcaaaaa ttgtgtacct
9001 ttagcttttt aatttgtaaa ggggttaata aggaatattt gatgtatagt gccttgacta
9061 gagatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa aaacctccca
9121 cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa cttgtttatt
9181 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
9241 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg
9301 atcaactgga taactcaagc taaccaaaat catcccaaac ttcccacccc ataccctatt
9361 accactgcca attacctagt ggtttcattt actctaaacc tgtgattcct ctgaattatt
9421 ttcattttaa agaaattgta tttgttaaat atgtactaca aacttagtag
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