质粒名称: | pX260-U6-DR-BB-DR-Cbh-NLS-hSpCas9-NLS-H1-shorttracr-PGK-puro |
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出品公司: | 爱迪基因 |
目录编号: | 42229 |
存储实验室: | Feng Zhang |
载体骨架基本信息 |
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载体名称: | pUC ori vector |
载体抗性: | 氨苄霉素 |
载体类型: | 哺乳细胞表达,CRISPR |
筛选标记: | 嘌呤霉素 |
高拷贝/低拷贝: | 高拷贝 |
生长条件: | 37 ℃ |
插入基因信息 |
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插入基因名称: | humanized S. pyogenes Cas9 |
插入大小: | 4272 bp |
标签/融合蛋白: | 3xFLAG(N端) |
文献引用方法 |
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材料和方法部分: | pX260-U6-DR-BB-DR-Cbh-NLS-hSpCas9-NLS-H1-shorttracr-PGK-puro was a gift from Feng Zhang (Addgene plasmid # 42229) |
参考文献部分: | Multiplex Genome Engineering Using CRISPR/Cas Systems. Cong L, Ran FA, Cox D, Lin S, Barretto R, Habib N, Hsu PD, Wu X, Jiang W, Marraffini LA, Zhang F. Science. 2013 Jan 3. 10.1126/science.1231143 PubMed 23287718 |
pX260-U6-DR-BB-DR-Cbh-NLS-hSpCas9-NLS-H1-shorttracr-PGK-puro
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载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pX260-U6-DR-BB-DR-Cbh-NLS-hSpCas9-NLS-H1-shorttracr-PGK-puro Sequencing Result 10203 bp DNA SYN DEFINITION pX260-U6-DR-BB-DR-Cbh-NLS-hSpCas9-NLS-H1-shorttracr-PGK-puro Sequencing Result ACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors. FEATURES Location/Qualifiers source 1..10203 /organism="pX260-U6-DR-BB-DR-Cbh-NLS-hSpCas9-NLS-H1-shorttracr-PGK-puro Sequencing Result" /mol_type="other DNA" misc_feature complement(168..437) /label="CAG_enhancer" misc_feature complement(641..660) /label="LKO1_5_primer" rep_origin complement(878..1497) /label="pBR322_origin" gene complement(1652..2512) /label="Ampicillin" /gene="Ampicillin" CDS complement(1652..2512) /label="ORF frame 3" promoter complement(2554..2582) /label="AmpR_promoter" misc_feature complement(2741..2763) /label="pGEX_3_primer" rep_origin complement(3031..3337) /label="f1_origin" CDS complement(3538..4485) /label="ORF frame 1" CDS 3576..4253 /label="ORF frame 3" gene complement(3627..4226) /label="puro (variant)" /gene="puro (variant)" CDS complement(3627..4226) /label="ORF frame 2" misc_feature complement(4314..4333) /label="mPGK_F_primer" misc_feature 4707..4730 /label="MSCV_rev_primer" misc_feature 4826..4859 /label="loxP" misc_feature complement(4935..4956) /label="EGFP_C_primer" misc_feature complement(5179..5198) /label="H1_primer" terminator complement(5354..5557) /label="bGH_PA_terminator" misc_feature 5543..5560 /label="BGH_rev_primer" CDS complement(5585..9856) /label="ORF frame 3" CDS 7220..9877 /label="ORF frame 2" misc_feature complement(9758..9781) /label="SV40 NLS" misc_feature complement(9761..9781) /label="SV40 NLS" misc_feature complement(9761..9775) /label="NLS" misc_feature complement(9788..9811) /label="FLAG" misc_feature complement(9812..9832) /label="FLAG" misc_feature complement(9833..9853) /label="FLAG" ORIGIN 1 TCTCCGCCTC GCCCCGCCCC GCCCCTCGCC CCGCCCCCCC CCCCCCCCCG CCCCCATCGC 61 TGCACAAAAT AATTAAAAAA TAAATAAATA CAAAATTGGG GGTGGGGAGG GGGGGGAGAT 121 GGGGAGAGTG AAGCAGAACG TGGGGCTCAC CTCGACCATG GTAATAGCGA TGACTAATAC 181 GTAGATGTAC TGCCAAGTAG GAAAGTCCCA TAAGGTCATG TACTGGGCAC AATGCCAGGC 241 GGGCCATTTA CCGTCATTGA CGTCAATAGG GGGCGTACTT GGCATATGAT ACACTTGATG 301 TACTGCCAAG TGGGCAGTTT ACCGTAAATA CTCCACCCAT TGACGTCAAT GGAAAGTCCC 361 TATTGGCGTT ACTATTGACG TCAATGGGCG GGGGTCGTTG GGCGGTCAGC CAGGCGGGCC 421 ATTTACCGTA AGTTATGTAA CGGGTACCTC TAGAGCCATT TGTCTGCAGA ATTGGCGCAC 481 GCGCTAGAAA AAGTTTTGGG ACCATTCAAA ACAGCATAGC TCTAAAACGT CTTCTCGAAG 541 ACCCGTTTTG GGACCATTCA AAACAGCATA GCTCTAAAAC CCGGTGTTTC GTCCTTTCCA 601 CAAGATATAT AAAGCCAAGA AATCGAAATA CTTTCAAGTT ACGGTAAGCA TATGATAGTC 661 CATTTTAAAA CATAATTTTA AAACTGCAAA CTACCCAAGA AATTATTACT TTCTACGTCA 721 CGTATTTTGT ACTAATATCT TTGTGTTTAC AGTCAAATTA ATTCCAATTA TCTCTCTAAC 781 AGCCTTGTAT CGTATATGCA AATATGAAGG AATCATGGGA AATAGGCCCT CACATGTGAG 841 CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA 901 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC 961 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG 1021 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC 1081 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG 1141 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC 1201 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA 1261 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG 1321 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA 1381 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG 1441 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT 1501 CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT 1561 TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT 1621 AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG CTTAATCAGT GAGGCACCTA 1681 TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG ACTCCCCGTC GTGTAGATAA 1741 CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC AATGATACCG CGGCTTCCAC 1801 GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC CGGAAGGGCC GAGCGCAGAA 1861 GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA TTGTTGCCGG GAAGCTAGAG 1921 TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC CATTGCTACA GGCATCGTGG 1981 TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG TTCCCAACGA TCAAGGCGAG 2041 TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC CTTCGGTCCT CCGATCGTTG 2101 TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT GGCAGCACTG CATAATTCTC 2161 TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG TGAGTACTCA ACCAAGTCAT 2221 TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC GGCGTCAATA CGGGATAATA 2281 CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG AAAACGTTCT TCGGGGCGAA 2341 AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT GTAACCCACT CGTGCACCCA 2401 ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG GTGAGCAAAA ACAGGAAGGC 2461 AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG TTGAATACTC ATACTCTTCC 2521 TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG 2581 AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC 2641 CTGACGTCTA AGAAACCATT ATTATCATGA CATTAACCTA TAAAAATAGG CGTATCACGA 2701 GGCCCTTTCG TCTCGCGCGT TTCGGTGATG ACGGTGAAAA CCTCTGACAC ATGCAGCTCC 2761 CGGAGACGGT CACAGCTTGT CTGTAAGCGG ATGCCGGGAG CAGACAAGCC CGTCAGGGCG 2821 CGTCAGCGGG TGTTGGCGGG TGTCGGGGCT GGCTTAACTA TGCGGCATCA GAGCAGATTG 2881 TACTGAGAGT GCACCATAAA ATTGTAAACG TTAATATTTT GTTAAAATTC GCGTTAAATT 2941 TTTGTTAAAT CAGCTCATTT TTTAACCAAT AGACCGAAAT CGGCAAAATC CCTTATAAAT 3001 CAAAAGAATA GCCCGAGATA GAGTTGAGTG TTGTTCCAGT TTGGAACAAG AGTCCACTAT 3061 TAAAGAACGT GGACTCCAAC GTCAAAGGGC GAAAAACCGT CTATCAGGGC GATGGCCCAC 3121 TACGTGAACC ATCACCCAAA TCAAGTTTTT TGGGGTCGAG GTGCCGTAAA GCACTAAATC 3181 GGAACCCTAA AGGGAGCCCC CGATTTAGAG CTTGACGGGG AAAGCCGGCG AACGTGGCGA 3241 GAAAGGAAGG GAAGAAAGCG AAAGGAGCGG GCGCTAAGGC GCTGGCAAGT GTAGCGGTCA 3301 CGCTGCGCGT AACCACCACA CCCGCCGCGC TTAATGCGCC GCTACAGGGC GCGTACTATG 3361 GTTGCTTTGA CGTATGCGGT GTGAAATACC GCACAGATGC GTAAGGAGAA AATACCGCAT 3421 CAGGCGCCGC TCCAATCCTT CCATTCAAAA TGTAGGTACT CTGTTCTCAC CCTTCTTAAC 3481 AAAGTATGAC AGGAAAAACT TCCATTTTAG TGGACATCTT TATTGTTTAA TAGATCATCA 3541 ATTTCTGCAT CCCGGGGATC TGATATCATC GATGCATGGG GTCGTGCGCT CCTTTCGGTC 3601 GGGCGCTGCG GGTCGTGGGG CGGGCGTCAG GCACCGGGCT TGCGGGTCAT GCACCAGGTG 3661 CGCGGTCCTT CGGGCACCTC GACGTCGGCG GTGACGGTGA AGCCGAGCCG CTCGTAGAAG 3721 GGGAGGTTGT GGGGCGCGGA GGTCTCCAGG AAGGCGGGCA CCCCGGCGCG CTCGGCCGCC 3781 TCCACTCCGG GGAGCACGAC GGCGCTGCCC AGACCCTTGC CCTGGTGGTC GGGCGAGACG 3841 CCGACGGTGG CCAGGAACCA CGCGGGCTCC TTGGGCCGGT GCGGCGCCAG GAGGCCTTCC 3901 ATCTGTTGCT GCGCGGCCAG CCGGGAACCG CTCAACTCGG CCATGCGCGG GCCGATCTCG 3961 GCGAACACCG CCCCCGCTTC GACGCTCTCC GGCGTGGTCC AGACCGCCAC CGCGGCGCCG 4021 TCGTCCGCGA CCCACACCTT GCCGATGTCG AGCCCGACGC GCGTGAGGAA GAGTTCTTGC 4081 AGCTCGGTGA CCCGCTCGAT GTGGCGGTCC GGATCGACGG TGTGGCGCGT GGCGGGGTAG 4141 TCGGCGAACG CGGCGGCGAG GGTGCGTACG GCCCTGGGGA CGTCGTCGCG GGTGGCGAGG 4201 CGCACCGTGG GCTTGTACTC GGTCATGGTA AGCTTCAGCT GCTCGAGATC TAGATGGATG 4261 CAGGTCGAAA GGCCCGGAGA TGAGGAAGAG GAGAACAGCG CGGCAGACGT GCGCTTTTGA 4321 AGCGTGCAGA ATGCCGGGCC TCCGGAGGAC CTTCGGGCGC CCGCCCCGCC CCTGAGCCCG 4381 CCCCTGAGCC CGCCCCCGGA CCCACCCCTT CCCAGCCTCT GAGCCCAGAA AGCGAAGGAG 4441 CAAAGCTGCT ATTGGCCGCT GCCCCAAAGG CCTACCCGCT TCCATTGCTC AGCGGTGCTG 4501 TCCATCTGCA CGAGACTAGT GAGACGTGCT ACTTCCATTT GTCACGTCCT GCACGACGCG 4561 AGCTGCGGGG CGGGGGGGAA CTTCCTGACT AGGGGAGGAG TAGAAGGTGG CGCGAAGGGG 4621 CCACCAAAGA ACGGAGCCGG TTGGCGCCTA CCGGTGGATG TGGAATGTGT GCGAGGCCAG 4681 AGGCCACTTG TGTAGCGCCA AGTGCCCAGC GGGGCTGCTA AAGCGCATGC TCCAGACTGC 4741 CTTGGGAAAA GCGCCTCCCC TACCCGGTAG AATTTCGAGG TCGAGATCCT AAGCTTGGCT 4801 GGACGTAAAC TCCTCTTCAG ACCTAATAAC TTCGTATAGC ATACATTATA CGAAGTTATA 4861 TTAAGGGTTA TTGAATATGA TCGGAATTCT TACTTGTACA GCTCGTCCAT GCCGAGAGTG 4921 ATCCCGGCGG CGGTCACGAA CTCCAGCAGG ACCATGTGAT CGCGCTTCTC GTTGGGGTCT 4981 TTGCTCAGGG CGGACTGGGT GCTCAGGTAG TGGTTGTCGG CGCCAAAAAG CACCGACTCG 5041 GTGCCACTTT TTCAAGTTGA TAACGGACTA GCCTTATTTT AACTTGCTAT GCTGTTTTGA 5101 ATGGTTCCAA CTAGATCTGT GGTCTCATAC AGAACTTATA AGATTCCCAA ATCCAAAGAC 5161 ATTTCACGTT TATGGTGATT TCCCAGAACA CATAGCGACA TGCAAATATT GCAGGGCGCC 5221 ACTCCCCTGT CCCTCACAGC CATCTTCCTG CCAGGGCGCA CGCGCGCTGG GTGTTCCCGC 5281 CTAGTGACAC TGGGCCCGCG ATTCCTTGGA GCGGGTTGAT GACGTCAGCG TTCGAATTGC 5341 GGCCGCTCCC CAGCATGCCT GCTATTCTCT TCCCAATCCT CCCCCTTGCT GTCCTGCCCC 5401 ACCCCACCCC CCAGAATAGA ATGACACCTA CTCAGACAAT GCGATGCAAT TTCCTCATTT 5461 TATTAGGAAA GGACAGTGGG AGTGGCACCT TCCAGGGTCA AGGAAGGCAC GGGGGAGGGG 5521 CAAACAACAG ATGGCTGGCA ACTAGAAGGC ACAGTCGAGG CTGATCAGCG AGCTCTAGGA 5581 ATTCTTACTT TTTCTTTTTT GCCTGGCCGG CCTTTTTCGT GGCCGCCGGC CTTTTGTCGC 5641 CTCCCAGCTG AGACAGGTCG ATCCGTGTCT CGTACAGGCC GGTGATGCTC TGGTGGATCA 5701 GGGTGGCGTC CAGCACCTCT TTGGTGCTGG TGTACCTCTT CCGGTCGATG GTGGTGTCAA 5761 AGTACTTGAA GGCGGCAGGG GCTCCCAGAT TGGTCAGGGT AAACAGGTGG ATGATATTCT 5821 CGGCCTGCTC TCTGATGGGC TTATCCCGGT GCTTGTTGTA GGCGGACAGC ACTTTGTCCA 5881 GATTAGCGTC GGCCAGGATC ACTCTCTTGG AGAACTCGCT GATCTGCTCG ATGATCTCGT 5941 CCAGGTAGTG CTTGTGCTGT TCCACAAACA GCTGTTTCTG CTCATTATCC TCGGGGGAGC 6001 CCTTCAGCTT CTCATAGTGG CTGGCCAGGT ACAGGAAGTT CACATATTTG GAGGGCAGGG 6061 CCAGTTCGTT TCCCTTCTGC AGTTCGCCGG CAGAGGCCAG CATTCTCTTC CGGCCGTTTT 6121 CCAGCTCGAA CAGGGAGTAC TTAGGCAGCT TGATGATCAG GTCCTTTTTC ACTTCTTTGT 6181 AGCCCTTGGC TTCCAGAAAG TCGATGGGAT TCTTCTCGAA GCTGCTTCTT TCCATGATGG 6241 TGATCCCCAG CAGCTCTTTC ACACTCTTCA GTTTCTTGGA CTTGCCCTTT TCCACTTTGG 6301 CCACCACCAG CACAGAATAG GCCACGGTGG GGCTGTCGAA GCCGCCGTAC TTCTTAGGGT 6361 CCCAGTCCTT CTTTCTGGCG ATCAGCTTAT CGCTGTTCCT CTTGGGCAGG ATAGACTCTT 6421 TGCTGAAGCC GCCTGTCTGC ACCTCGGTCT TTTTCACGAT ATTCACTTGG GGCATGCTCA 6481 GCACTTTCCG CACGGTGGCA AAATCCCGGC CCTTATCCCA CACGATCTCC CCGGTTTCGC 6541 CGTTTGTCTC GATCAGAGGC CGCTTCCGGA TCTCGCCGTT GGCCAGGGTA ATCTCGGTCT 6601 TGAAAAAGTT CATGATGTTG CTGTAGAAGA AGTACTTGGC GGTAGCCTTG CCGATTTCCT 6661 GCTCGCTCTT GGCGATCATC TTCCGCACGT CGTACACCTT GTAGTCGCCG TACACGAACT 6721 CGCTTTCCAG CTTAGGGTAC TTTTTGATCA GGGCGGTTCC CACGACGGCG TTCAGGTAGG 6781 CGTCGTGGGC GTGGTGGTAG TTGTTGATCT CGCGCACTTT GTAAAACTGG AAATCCTTCC 6841 GGAAATCGGA CACCAGCTTG GACTTCAGGG TGATCACTTT CACTTCCCGG ATCAGCTTGT 6901 CATTCTCGTC GTACTTAGTG TTCATCCGGG AGTCCAGGAT CTGTGCCACG TGCTTTGTGA 6961 TCTGCCGGGT TTCCACCAGC TGTCTCTTGA TGAAGCCGGC CTTATCCAGT TCGCTCAGGC 7021 CGCCTCTCTC GGCCTTGGTC AGATTGTCGA ACTTTCTCTG GGTAATCAGC TTGGCGTTCA 7081 GCAGCTGCCG CCAGTAGTTC TTCATCTTCT TCACGACCTC TTCGGAGGGC ACGTTGTCGC 7141 TCTTGCCCCG GTTCTTGTCG CTTCTGGTCA GCACCTTGTT GTCGATGGAG TCGTCCTTCA 7201 GAAAGCTCTG AGGCACGATA TGGTCCACAT CGTAGTCGGA CAGCCGGTTG ATGTCCAGTT 7261 CCTGGTCCAC GTACATATCC CGCCCATTCT GCAGGTAGTA CAGGTACAGC TTCTCGTTCT 7321 GCAGCTGGGT GTTTTCCACG GGGTGTTCTT TCAGGATCTG GCTGCCCAGC TCTTTGATGC 7381 CCTCTTCGAT CCGCTTCATT CTCTCGCGGC TGTTCTTCTG TCCCTTCTGG GTGGTCTGGT 7441 TCTCTCTGGC CATTTCGATC ACGATGTTCT CGGGCTTGTG CCGGCCCATC ACTTTCACGA 7501 GCTCGTCCAC CACCTTCACT GTCTGCAGGA TGCCCTTCTT AATGGCGGGG CTGCCGGCCA 7561 GATTGGCAAT GTGCTCGTGC AGGCTATCGC CCTGGCCGGA CACCTGGGCT TTCTGGATGT 7621 CCTCTTTAAA GGTCAGGCTG TCGTCGTGGA TCAGCTGCAT GAAGTTTCTG TTGGCGAAGC 7681 CGTCGGACTT CAGGAAATCC AGGATTGTCT TGCCGGACTG CTTGTCCCGG ATGCCGTTGA 7741 TCAGCTTCCG GCTCAGCCTG CCCCAGCCGG TGTATCTCCG CCGCTTCAGC TGCTTCATCA 7801 CTTTGTCGTC GAACAGGTGG GCATAGGTTT TCAGCCGTTC CTCGATCATC TCTCTGTCCT 7861 CAAACAGTGT CAGGGTCAGC ACGATATCTT CCAGAATGTC CTCGTTTTCC TCATTGTCCA 7921 GGAAGTCCTT GTCCTTGATA ATTTTCAGCA GATCGTGGTA TGTGCCCAGG GAGGCGTTGA 7981 ACCGATCTTC CACGCCGGAG ATTTCCACGG AGTCGAAGCA CTCGATTTTC TTGAAGTAGT 8041 CCTCTTTCAG CTGCTTCACG GTCACTTTCC GGTTGGTCTT GAACAGCAGG TCCACGATGG 8101 CCTTTTTCTG CTCGCCGCTC AGGAAGGCGG GCTTTCTCAT TCCCTCGGTC ACGTATTTCA 8161 CTTTGGTCAG CTCGTTATAC ACGGTGAAGT ACTCGTACAG CAGGCTGTGC TTGGGCAGCA 8221 CCTTCTCGTT GGGCAGGTTC TTATCGAAGT TGGTCATCCG CTCGATGAAG CTCTGGGCGG 8281 AAGCGCCCTT GTCCACCACT TCCTCGAAGT TCCAGGGGGT GATGGTTTCC TCGCTCTTTC 8341 TGGTCATCCA GGCGAATCTG CTGTTTCCCC TGGCCAGAGG GCCCACGTAG TAGGGGATGC 8401 GGAAGGTCAG GATCTTCTCG ATCTTTTCCC GGTTGTCCTT CAGGAATGGG TAAAAATCTT 8461 CCTGCCGCCG CAGAATGGCG TGCAGCTCTC CCAGGTGGAT CTGGTGGGGG ATGCTGCCGT 8521 TGTCGAAGGT CCGCTGCTTC CGCAGCAGGT CCTCTCTGTT CAGCTTCACG AGCAGTTCCT 8581 CGGTGCCGTC CATCTTTTCC AGGATGGGCT TGATGAACTT GTAGAACTCT TCCTGGCTGG 8641 CTCCGCCGTC AATGTAGCCG GCGTAGCCGT TCTTGCTCTG GTCGAAGAAA ATCTCTTTGT 8701 ACTTCTCAGG CAGCTGCTGC CGCACGAGAG CTTTCAGCAG GGTCAGGTCC TGGTGGTGCT 8761 CGTCGTATCT CTTGATCATA GAGGCGCTCA GGGGGGCCTT GGTGATCTCG GTGTTCACTC 8821 TCAGGATGTC GCTCAGCAGG ATGGCGTCGG ACAGGTTCTT GGCGGCCAGA AACAGGTCGG 8881 CGTACTGGTC GCCGATCTGG GCCAGCAGGT TGTCCAGGTC GTCGTCGTAG GTGTCCTTGC 8941 TCAGCTGCAG TTTGGCATCC TCGGCCAGGT CGAAGTTGCT CTTGAAGTTG GGGGTCAGGC 9001 CCAGGCTCAG GGCAATCAGG TTTCCGAACA GGCCATTCTT CTTCTCGCCG GGCAGCTGGG 9061 CGATCAGATT TTCCAGCCGT CTGCTCTTGC TCAGTCTGGC AGACAGGATG GCCTTGGCGT 9121 CCACGCCGCT GGCGTTGATG GGGTTTTCCT CGAACAGCTG GTTGTAGGTC TGCACCAGCT 9181 GGATGAACAG CTTGTCCACG TCGCTGTTGT CGGGGTTCAG GTCGCCCTCG ATCAGGAAGT 9241 GGCCCCGGAA CTTGATCATG TGGGCCAGGG CCAGATAGAT CAGCCGCAGG TCGGCCTTGT 9301 CGGTGCTGTC CACCAGTTTC TTTCTCAGGT GGTAGATGGT GGGGTACTTC TCGTGGTAGG 9361 CCACCTCGTC CACGATGTTG CCGAAGATGG GGTGCCGCTC GTGCTTCTTA TCCTCTTCCA 9421 CCAGGAAGGA CTCTTCCAGT CTGTGGAAGA AGCTGTCGTC CACCTTGGCC ATCTCGTTGC 9481 TGAAGATCTC TTGCAGATAG CAGATCCGGT TCTTCCGTCT GGTGTATCTT CTTCTGGCGG 9541 TTCTCTTCAG CCGGGTGGCC TCGGCTGTTT CGCCGCTGTC GAACAGCAGG GCTCCGATCA 9601 GGTTCTTCTT GATGCTGTGC CGGTCGGTGT TGCCCAGCAC CTTGAATTTC TTGCTGGGCA 9661 CCTTGTACTC GTCGGTGATC ACGGCCCAGC CCACAGAGTT GGTGCCGATG TCCAGGCCGA 9721 TGCTGTACTT CTTGTCGGCT GCTGGGACTC CGTGGATACC GACCTTCCGC TTCTTCTTTG 9781 GGGCCATCTT ATCGTCATCG TCTTTGTAAT CAATATCATG ATCCTTGTAG TCTCCGTCGT 9841 GGTCCTTATA GTCCATGGTG GCACCGGTCC AACCTGAAAA AAAGTGATTT CAGGCAGGTG 9901 CTCCAGGTAA TTAAACATTA ATACCCCACC AACCAACCAT CCCTTAAACC CTTACCTCTT 9961 GCTCAGCTAA TTACAGCCCG GAGGAGAAGG GCCGTCCCGC CCGCTCACCT GTGGGAGTAA 10021 CGCGGTCAGT CAGAGCCGGG GCGGGCGGCG CGAGGCGGCG GCGGAGCGGG GCACGGGGCG 10081 AAGGCAGCGC GCAGCGACTC CCGCCCGCCG CGCGCTTCGC TTTTTATAGG GCCGCCGCCG 10141 CCGCCGCCTC GCCATAAAAG GAAACTTTCG GAGCGCGCCG CTCTGATTGG CTGCCGCCGC 10201 ACC //
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