pX260-U6-DR-BB-DR-Cbh-NLS-hSpCas9-NLS-H1-shorttracr-PGK-puro

价格:1200元

联系方式:I47-825O-882O

相关技术服务:质粒构建    基因合成    质粒大提

买家导航

载体基本信息

质粒名称: pX260-U6-DR-BB-DR-Cbh-NLS-hSpCas9-NLS-H1-shorttracr-PGK-puro 
出品公司: 爱迪基因
目录编号: 42229
存储实验室: Feng Zhang

载体骨架基本信息
载体名称: pUC ori vector
载体抗性: 氨苄霉素
载体类型: 哺乳细胞表达,CRISPR
筛选标记: 嘌呤霉素
高拷贝/低拷贝: 高拷贝
生长条件: 37 ℃

插入基因信息
插入基因名称: humanized S. pyogenes Cas9
插入大小: 4272 bp
标签/融合蛋白: 3xFLAG(N端)

文献引用方法
材料和方法部分: pX260-U6-DR-BB-DR-Cbh-NLS-hSpCas9-NLS-H1-shorttracr-PGK-puro was a gift from Feng Zhang (Addgene plasmid # 42229)
参考文献部分: Multiplex Genome Engineering Using CRISPR/Cas Systems. Cong L, Ran FA, Cox D, Lin S, Barretto R, Habib N, Hsu PD, Wu X, Jiang W, Marraffini LA, Zhang F. Science. 2013 Jan 3. 10.1126/science.1231143 PubMed 23287718

载体图谱质粒图谱和多克隆位点信息

pX260-U6-DR-BB-DR-Cbh-NLS-hSpCas9-NLS-H1-shorttracr-PGK-puro载体图谱

载体简介

载体序列

LOCUS       pX260-U6-DR-BB-DR-Cbh-NLS-hSpCas9-NLS-H1-shorttracr-PGK-puro Sequencing Result	10203 bp 	DNA	SYN
DEFINITION  pX260-U6-DR-BB-DR-Cbh-NLS-hSpCas9-NLS-H1-shorttracr-PGK-puro Sequencing Result
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..10203
                     /organism="pX260-U6-DR-BB-DR-Cbh-NLS-hSpCas9-NLS-H1-shorttracr-PGK-puro Sequencing Result"
                     /mol_type="other DNA"
     misc_feature    complement(168..437)
                     /label="CAG_enhancer"
     misc_feature    complement(641..660)
                     /label="LKO1_5_primer"
     rep_origin      complement(878..1497)
                     /label="pBR322_origin"
     gene            complement(1652..2512)
                     /label="Ampicillin"
                     /gene="Ampicillin"
     CDS             complement(1652..2512)
                     /label="ORF frame 3"
     promoter        complement(2554..2582)
                     /label="AmpR_promoter"
     misc_feature    complement(2741..2763)
                     /label="pGEX_3_primer"
     rep_origin      complement(3031..3337)
                     /label="f1_origin"
     CDS             complement(3538..4485)
                     /label="ORF frame 1"
     CDS             3576..4253
                     /label="ORF frame 3"
     gene            complement(3627..4226)
                     /label="puro (variant)"
                     /gene="puro (variant)"
     CDS             complement(3627..4226)
                     /label="ORF frame 2"
     misc_feature    complement(4314..4333)
                     /label="mPGK_F_primer"
     misc_feature    4707..4730
                     /label="MSCV_rev_primer"
     misc_feature    4826..4859
                     /label="loxP"
     misc_feature    complement(4935..4956)
                     /label="EGFP_C_primer"
     misc_feature    complement(5179..5198)
                     /label="H1_primer"
     terminator      complement(5354..5557)
                     /label="bGH_PA_terminator"
     misc_feature    5543..5560
                     /label="BGH_rev_primer"
     CDS             complement(5585..9856)
                     /label="ORF frame 3"
     CDS             7220..9877
                     /label="ORF frame 2"
     misc_feature    complement(9758..9781)
                     /label="SV40 NLS"
     misc_feature    complement(9761..9781)
                     /label="SV40 NLS"
     misc_feature    complement(9761..9775)
                     /label="NLS"
     misc_feature    complement(9788..9811)
                     /label="FLAG"
     misc_feature    complement(9812..9832)
                     /label="FLAG"
     misc_feature    complement(9833..9853)
                     /label="FLAG"
ORIGIN
    1 TCTCCGCCTC GCCCCGCCCC GCCCCTCGCC CCGCCCCCCC CCCCCCCCCG CCCCCATCGC
   61 TGCACAAAAT AATTAAAAAA TAAATAAATA CAAAATTGGG GGTGGGGAGG GGGGGGAGAT
  121 GGGGAGAGTG AAGCAGAACG TGGGGCTCAC CTCGACCATG GTAATAGCGA TGACTAATAC
  181 GTAGATGTAC TGCCAAGTAG GAAAGTCCCA TAAGGTCATG TACTGGGCAC AATGCCAGGC
  241 GGGCCATTTA CCGTCATTGA CGTCAATAGG GGGCGTACTT GGCATATGAT ACACTTGATG
  301 TACTGCCAAG TGGGCAGTTT ACCGTAAATA CTCCACCCAT TGACGTCAAT GGAAAGTCCC
  361 TATTGGCGTT ACTATTGACG TCAATGGGCG GGGGTCGTTG GGCGGTCAGC CAGGCGGGCC
  421 ATTTACCGTA AGTTATGTAA CGGGTACCTC TAGAGCCATT TGTCTGCAGA ATTGGCGCAC
  481 GCGCTAGAAA AAGTTTTGGG ACCATTCAAA ACAGCATAGC TCTAAAACGT CTTCTCGAAG
  541 ACCCGTTTTG GGACCATTCA AAACAGCATA GCTCTAAAAC CCGGTGTTTC GTCCTTTCCA
  601 CAAGATATAT AAAGCCAAGA AATCGAAATA CTTTCAAGTT ACGGTAAGCA TATGATAGTC
  661 CATTTTAAAA CATAATTTTA AAACTGCAAA CTACCCAAGA AATTATTACT TTCTACGTCA
  721 CGTATTTTGT ACTAATATCT TTGTGTTTAC AGTCAAATTA ATTCCAATTA TCTCTCTAAC
  781 AGCCTTGTAT CGTATATGCA AATATGAAGG AATCATGGGA AATAGGCCCT CACATGTGAG
  841 CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA
  901 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC
  961 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG
 1021 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC
 1081 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG
 1141 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC
 1201 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA
 1261 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG
 1321 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA
 1381 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG
 1441 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT
 1501 CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT
 1561 TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT
 1621 AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG CTTAATCAGT GAGGCACCTA
 1681 TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG ACTCCCCGTC GTGTAGATAA
 1741 CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC AATGATACCG CGGCTTCCAC
 1801 GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC CGGAAGGGCC GAGCGCAGAA
 1861 GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA TTGTTGCCGG GAAGCTAGAG
 1921 TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC CATTGCTACA GGCATCGTGG
 1981 TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG TTCCCAACGA TCAAGGCGAG
 2041 TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC CTTCGGTCCT CCGATCGTTG
 2101 TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT GGCAGCACTG CATAATTCTC
 2161 TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG TGAGTACTCA ACCAAGTCAT
 2221 TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC GGCGTCAATA CGGGATAATA
 2281 CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG AAAACGTTCT TCGGGGCGAA
 2341 AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT GTAACCCACT CGTGCACCCA
 2401 ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG GTGAGCAAAA ACAGGAAGGC
 2461 AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG TTGAATACTC ATACTCTTCC
 2521 TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG
 2581 AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC
 2641 CTGACGTCTA AGAAACCATT ATTATCATGA CATTAACCTA TAAAAATAGG CGTATCACGA
 2701 GGCCCTTTCG TCTCGCGCGT TTCGGTGATG ACGGTGAAAA CCTCTGACAC ATGCAGCTCC
 2761 CGGAGACGGT CACAGCTTGT CTGTAAGCGG ATGCCGGGAG CAGACAAGCC CGTCAGGGCG
 2821 CGTCAGCGGG TGTTGGCGGG TGTCGGGGCT GGCTTAACTA TGCGGCATCA GAGCAGATTG
 2881 TACTGAGAGT GCACCATAAA ATTGTAAACG TTAATATTTT GTTAAAATTC GCGTTAAATT
 2941 TTTGTTAAAT CAGCTCATTT TTTAACCAAT AGACCGAAAT CGGCAAAATC CCTTATAAAT
 3001 CAAAAGAATA GCCCGAGATA GAGTTGAGTG TTGTTCCAGT TTGGAACAAG AGTCCACTAT
 3061 TAAAGAACGT GGACTCCAAC GTCAAAGGGC GAAAAACCGT CTATCAGGGC GATGGCCCAC
 3121 TACGTGAACC ATCACCCAAA TCAAGTTTTT TGGGGTCGAG GTGCCGTAAA GCACTAAATC
 3181 GGAACCCTAA AGGGAGCCCC CGATTTAGAG CTTGACGGGG AAAGCCGGCG AACGTGGCGA
 3241 GAAAGGAAGG GAAGAAAGCG AAAGGAGCGG GCGCTAAGGC GCTGGCAAGT GTAGCGGTCA
 3301 CGCTGCGCGT AACCACCACA CCCGCCGCGC TTAATGCGCC GCTACAGGGC GCGTACTATG
 3361 GTTGCTTTGA CGTATGCGGT GTGAAATACC GCACAGATGC GTAAGGAGAA AATACCGCAT
 3421 CAGGCGCCGC TCCAATCCTT CCATTCAAAA TGTAGGTACT CTGTTCTCAC CCTTCTTAAC
 3481 AAAGTATGAC AGGAAAAACT TCCATTTTAG TGGACATCTT TATTGTTTAA TAGATCATCA
 3541 ATTTCTGCAT CCCGGGGATC TGATATCATC GATGCATGGG GTCGTGCGCT CCTTTCGGTC
 3601 GGGCGCTGCG GGTCGTGGGG CGGGCGTCAG GCACCGGGCT TGCGGGTCAT GCACCAGGTG
 3661 CGCGGTCCTT CGGGCACCTC GACGTCGGCG GTGACGGTGA AGCCGAGCCG CTCGTAGAAG
 3721 GGGAGGTTGT GGGGCGCGGA GGTCTCCAGG AAGGCGGGCA CCCCGGCGCG CTCGGCCGCC
 3781 TCCACTCCGG GGAGCACGAC GGCGCTGCCC AGACCCTTGC CCTGGTGGTC GGGCGAGACG
 3841 CCGACGGTGG CCAGGAACCA CGCGGGCTCC TTGGGCCGGT GCGGCGCCAG GAGGCCTTCC
 3901 ATCTGTTGCT GCGCGGCCAG CCGGGAACCG CTCAACTCGG CCATGCGCGG GCCGATCTCG
 3961 GCGAACACCG CCCCCGCTTC GACGCTCTCC GGCGTGGTCC AGACCGCCAC CGCGGCGCCG
 4021 TCGTCCGCGA CCCACACCTT GCCGATGTCG AGCCCGACGC GCGTGAGGAA GAGTTCTTGC
 4081 AGCTCGGTGA CCCGCTCGAT GTGGCGGTCC GGATCGACGG TGTGGCGCGT GGCGGGGTAG
 4141 TCGGCGAACG CGGCGGCGAG GGTGCGTACG GCCCTGGGGA CGTCGTCGCG GGTGGCGAGG
 4201 CGCACCGTGG GCTTGTACTC GGTCATGGTA AGCTTCAGCT GCTCGAGATC TAGATGGATG
 4261 CAGGTCGAAA GGCCCGGAGA TGAGGAAGAG GAGAACAGCG CGGCAGACGT GCGCTTTTGA
 4321 AGCGTGCAGA ATGCCGGGCC TCCGGAGGAC CTTCGGGCGC CCGCCCCGCC CCTGAGCCCG
 4381 CCCCTGAGCC CGCCCCCGGA CCCACCCCTT CCCAGCCTCT GAGCCCAGAA AGCGAAGGAG
 4441 CAAAGCTGCT ATTGGCCGCT GCCCCAAAGG CCTACCCGCT TCCATTGCTC AGCGGTGCTG
 4501 TCCATCTGCA CGAGACTAGT GAGACGTGCT ACTTCCATTT GTCACGTCCT GCACGACGCG
 4561 AGCTGCGGGG CGGGGGGGAA CTTCCTGACT AGGGGAGGAG TAGAAGGTGG CGCGAAGGGG
 4621 CCACCAAAGA ACGGAGCCGG TTGGCGCCTA CCGGTGGATG TGGAATGTGT GCGAGGCCAG
 4681 AGGCCACTTG TGTAGCGCCA AGTGCCCAGC GGGGCTGCTA AAGCGCATGC TCCAGACTGC
 4741 CTTGGGAAAA GCGCCTCCCC TACCCGGTAG AATTTCGAGG TCGAGATCCT AAGCTTGGCT
 4801 GGACGTAAAC TCCTCTTCAG ACCTAATAAC TTCGTATAGC ATACATTATA CGAAGTTATA
 4861 TTAAGGGTTA TTGAATATGA TCGGAATTCT TACTTGTACA GCTCGTCCAT GCCGAGAGTG
 4921 ATCCCGGCGG CGGTCACGAA CTCCAGCAGG ACCATGTGAT CGCGCTTCTC GTTGGGGTCT
 4981 TTGCTCAGGG CGGACTGGGT GCTCAGGTAG TGGTTGTCGG CGCCAAAAAG CACCGACTCG
 5041 GTGCCACTTT TTCAAGTTGA TAACGGACTA GCCTTATTTT AACTTGCTAT GCTGTTTTGA
 5101 ATGGTTCCAA CTAGATCTGT GGTCTCATAC AGAACTTATA AGATTCCCAA ATCCAAAGAC
 5161 ATTTCACGTT TATGGTGATT TCCCAGAACA CATAGCGACA TGCAAATATT GCAGGGCGCC
 5221 ACTCCCCTGT CCCTCACAGC CATCTTCCTG CCAGGGCGCA CGCGCGCTGG GTGTTCCCGC
 5281 CTAGTGACAC TGGGCCCGCG ATTCCTTGGA GCGGGTTGAT GACGTCAGCG TTCGAATTGC
 5341 GGCCGCTCCC CAGCATGCCT GCTATTCTCT TCCCAATCCT CCCCCTTGCT GTCCTGCCCC
 5401 ACCCCACCCC CCAGAATAGA ATGACACCTA CTCAGACAAT GCGATGCAAT TTCCTCATTT
 5461 TATTAGGAAA GGACAGTGGG AGTGGCACCT TCCAGGGTCA AGGAAGGCAC GGGGGAGGGG
 5521 CAAACAACAG ATGGCTGGCA ACTAGAAGGC ACAGTCGAGG CTGATCAGCG AGCTCTAGGA
 5581 ATTCTTACTT TTTCTTTTTT GCCTGGCCGG CCTTTTTCGT GGCCGCCGGC CTTTTGTCGC
 5641 CTCCCAGCTG AGACAGGTCG ATCCGTGTCT CGTACAGGCC GGTGATGCTC TGGTGGATCA
 5701 GGGTGGCGTC CAGCACCTCT TTGGTGCTGG TGTACCTCTT CCGGTCGATG GTGGTGTCAA
 5761 AGTACTTGAA GGCGGCAGGG GCTCCCAGAT TGGTCAGGGT AAACAGGTGG ATGATATTCT
 5821 CGGCCTGCTC TCTGATGGGC TTATCCCGGT GCTTGTTGTA GGCGGACAGC ACTTTGTCCA
 5881 GATTAGCGTC GGCCAGGATC ACTCTCTTGG AGAACTCGCT GATCTGCTCG ATGATCTCGT
 5941 CCAGGTAGTG CTTGTGCTGT TCCACAAACA GCTGTTTCTG CTCATTATCC TCGGGGGAGC
 6001 CCTTCAGCTT CTCATAGTGG CTGGCCAGGT ACAGGAAGTT CACATATTTG GAGGGCAGGG
 6061 CCAGTTCGTT TCCCTTCTGC AGTTCGCCGG CAGAGGCCAG CATTCTCTTC CGGCCGTTTT
 6121 CCAGCTCGAA CAGGGAGTAC TTAGGCAGCT TGATGATCAG GTCCTTTTTC ACTTCTTTGT
 6181 AGCCCTTGGC TTCCAGAAAG TCGATGGGAT TCTTCTCGAA GCTGCTTCTT TCCATGATGG
 6241 TGATCCCCAG CAGCTCTTTC ACACTCTTCA GTTTCTTGGA CTTGCCCTTT TCCACTTTGG
 6301 CCACCACCAG CACAGAATAG GCCACGGTGG GGCTGTCGAA GCCGCCGTAC TTCTTAGGGT
 6361 CCCAGTCCTT CTTTCTGGCG ATCAGCTTAT CGCTGTTCCT CTTGGGCAGG ATAGACTCTT
 6421 TGCTGAAGCC GCCTGTCTGC ACCTCGGTCT TTTTCACGAT ATTCACTTGG GGCATGCTCA
 6481 GCACTTTCCG CACGGTGGCA AAATCCCGGC CCTTATCCCA CACGATCTCC CCGGTTTCGC
 6541 CGTTTGTCTC GATCAGAGGC CGCTTCCGGA TCTCGCCGTT GGCCAGGGTA ATCTCGGTCT
 6601 TGAAAAAGTT CATGATGTTG CTGTAGAAGA AGTACTTGGC GGTAGCCTTG CCGATTTCCT
 6661 GCTCGCTCTT GGCGATCATC TTCCGCACGT CGTACACCTT GTAGTCGCCG TACACGAACT
 6721 CGCTTTCCAG CTTAGGGTAC TTTTTGATCA GGGCGGTTCC CACGACGGCG TTCAGGTAGG
 6781 CGTCGTGGGC GTGGTGGTAG TTGTTGATCT CGCGCACTTT GTAAAACTGG AAATCCTTCC
 6841 GGAAATCGGA CACCAGCTTG GACTTCAGGG TGATCACTTT CACTTCCCGG ATCAGCTTGT
 6901 CATTCTCGTC GTACTTAGTG TTCATCCGGG AGTCCAGGAT CTGTGCCACG TGCTTTGTGA
 6961 TCTGCCGGGT TTCCACCAGC TGTCTCTTGA TGAAGCCGGC CTTATCCAGT TCGCTCAGGC
 7021 CGCCTCTCTC GGCCTTGGTC AGATTGTCGA ACTTTCTCTG GGTAATCAGC TTGGCGTTCA
 7081 GCAGCTGCCG CCAGTAGTTC TTCATCTTCT TCACGACCTC TTCGGAGGGC ACGTTGTCGC
 7141 TCTTGCCCCG GTTCTTGTCG CTTCTGGTCA GCACCTTGTT GTCGATGGAG TCGTCCTTCA
 7201 GAAAGCTCTG AGGCACGATA TGGTCCACAT CGTAGTCGGA CAGCCGGTTG ATGTCCAGTT
 7261 CCTGGTCCAC GTACATATCC CGCCCATTCT GCAGGTAGTA CAGGTACAGC TTCTCGTTCT
 7321 GCAGCTGGGT GTTTTCCACG GGGTGTTCTT TCAGGATCTG GCTGCCCAGC TCTTTGATGC
 7381 CCTCTTCGAT CCGCTTCATT CTCTCGCGGC TGTTCTTCTG TCCCTTCTGG GTGGTCTGGT
 7441 TCTCTCTGGC CATTTCGATC ACGATGTTCT CGGGCTTGTG CCGGCCCATC ACTTTCACGA
 7501 GCTCGTCCAC CACCTTCACT GTCTGCAGGA TGCCCTTCTT AATGGCGGGG CTGCCGGCCA
 7561 GATTGGCAAT GTGCTCGTGC AGGCTATCGC CCTGGCCGGA CACCTGGGCT TTCTGGATGT
 7621 CCTCTTTAAA GGTCAGGCTG TCGTCGTGGA TCAGCTGCAT GAAGTTTCTG TTGGCGAAGC
 7681 CGTCGGACTT CAGGAAATCC AGGATTGTCT TGCCGGACTG CTTGTCCCGG ATGCCGTTGA
 7741 TCAGCTTCCG GCTCAGCCTG CCCCAGCCGG TGTATCTCCG CCGCTTCAGC TGCTTCATCA
 7801 CTTTGTCGTC GAACAGGTGG GCATAGGTTT TCAGCCGTTC CTCGATCATC TCTCTGTCCT
 7861 CAAACAGTGT CAGGGTCAGC ACGATATCTT CCAGAATGTC CTCGTTTTCC TCATTGTCCA
 7921 GGAAGTCCTT GTCCTTGATA ATTTTCAGCA GATCGTGGTA TGTGCCCAGG GAGGCGTTGA
 7981 ACCGATCTTC CACGCCGGAG ATTTCCACGG AGTCGAAGCA CTCGATTTTC TTGAAGTAGT
 8041 CCTCTTTCAG CTGCTTCACG GTCACTTTCC GGTTGGTCTT GAACAGCAGG TCCACGATGG
 8101 CCTTTTTCTG CTCGCCGCTC AGGAAGGCGG GCTTTCTCAT TCCCTCGGTC ACGTATTTCA
 8161 CTTTGGTCAG CTCGTTATAC ACGGTGAAGT ACTCGTACAG CAGGCTGTGC TTGGGCAGCA
 8221 CCTTCTCGTT GGGCAGGTTC TTATCGAAGT TGGTCATCCG CTCGATGAAG CTCTGGGCGG
 8281 AAGCGCCCTT GTCCACCACT TCCTCGAAGT TCCAGGGGGT GATGGTTTCC TCGCTCTTTC
 8341 TGGTCATCCA GGCGAATCTG CTGTTTCCCC TGGCCAGAGG GCCCACGTAG TAGGGGATGC
 8401 GGAAGGTCAG GATCTTCTCG ATCTTTTCCC GGTTGTCCTT CAGGAATGGG TAAAAATCTT
 8461 CCTGCCGCCG CAGAATGGCG TGCAGCTCTC CCAGGTGGAT CTGGTGGGGG ATGCTGCCGT
 8521 TGTCGAAGGT CCGCTGCTTC CGCAGCAGGT CCTCTCTGTT CAGCTTCACG AGCAGTTCCT
 8581 CGGTGCCGTC CATCTTTTCC AGGATGGGCT TGATGAACTT GTAGAACTCT TCCTGGCTGG
 8641 CTCCGCCGTC AATGTAGCCG GCGTAGCCGT TCTTGCTCTG GTCGAAGAAA ATCTCTTTGT
 8701 ACTTCTCAGG CAGCTGCTGC CGCACGAGAG CTTTCAGCAG GGTCAGGTCC TGGTGGTGCT
 8761 CGTCGTATCT CTTGATCATA GAGGCGCTCA GGGGGGCCTT GGTGATCTCG GTGTTCACTC
 8821 TCAGGATGTC GCTCAGCAGG ATGGCGTCGG ACAGGTTCTT GGCGGCCAGA AACAGGTCGG
 8881 CGTACTGGTC GCCGATCTGG GCCAGCAGGT TGTCCAGGTC GTCGTCGTAG GTGTCCTTGC
 8941 TCAGCTGCAG TTTGGCATCC TCGGCCAGGT CGAAGTTGCT CTTGAAGTTG GGGGTCAGGC
 9001 CCAGGCTCAG GGCAATCAGG TTTCCGAACA GGCCATTCTT CTTCTCGCCG GGCAGCTGGG
 9061 CGATCAGATT TTCCAGCCGT CTGCTCTTGC TCAGTCTGGC AGACAGGATG GCCTTGGCGT
 9121 CCACGCCGCT GGCGTTGATG GGGTTTTCCT CGAACAGCTG GTTGTAGGTC TGCACCAGCT
 9181 GGATGAACAG CTTGTCCACG TCGCTGTTGT CGGGGTTCAG GTCGCCCTCG ATCAGGAAGT
 9241 GGCCCCGGAA CTTGATCATG TGGGCCAGGG CCAGATAGAT CAGCCGCAGG TCGGCCTTGT
 9301 CGGTGCTGTC CACCAGTTTC TTTCTCAGGT GGTAGATGGT GGGGTACTTC TCGTGGTAGG
 9361 CCACCTCGTC CACGATGTTG CCGAAGATGG GGTGCCGCTC GTGCTTCTTA TCCTCTTCCA
 9421 CCAGGAAGGA CTCTTCCAGT CTGTGGAAGA AGCTGTCGTC CACCTTGGCC ATCTCGTTGC
 9481 TGAAGATCTC TTGCAGATAG CAGATCCGGT TCTTCCGTCT GGTGTATCTT CTTCTGGCGG
 9541 TTCTCTTCAG CCGGGTGGCC TCGGCTGTTT CGCCGCTGTC GAACAGCAGG GCTCCGATCA
 9601 GGTTCTTCTT GATGCTGTGC CGGTCGGTGT TGCCCAGCAC CTTGAATTTC TTGCTGGGCA
 9661 CCTTGTACTC GTCGGTGATC ACGGCCCAGC CCACAGAGTT GGTGCCGATG TCCAGGCCGA
 9721 TGCTGTACTT CTTGTCGGCT GCTGGGACTC CGTGGATACC GACCTTCCGC TTCTTCTTTG
 9781 GGGCCATCTT ATCGTCATCG TCTTTGTAAT CAATATCATG ATCCTTGTAG TCTCCGTCGT
 9841 GGTCCTTATA GTCCATGGTG GCACCGGTCC AACCTGAAAA AAAGTGATTT CAGGCAGGTG
 9901 CTCCAGGTAA TTAAACATTA ATACCCCACC AACCAACCAT CCCTTAAACC CTTACCTCTT
 9961 GCTCAGCTAA TTACAGCCCG GAGGAGAAGG GCCGTCCCGC CCGCTCACCT GTGGGAGTAA
10021 CGCGGTCAGT CAGAGCCGGG GCGGGCGGCG CGAGGCGGCG GCGGAGCGGG GCACGGGGCG
10081 AAGGCAGCGC GCAGCGACTC CCGCCCGCCG CGCGCTTCGC TTTTTATAGG GCCGCCGCCG
10141 CCGCCGCCTC GCCATAAAAG GAAACTTTCG GAGCGCGCCG CTCTGATTGG CTGCCGCCGC
10201 ACC
//

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