| 质粒名称: | pXPR_047 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 107145 |
| 存储实验室: | David Root |
|
载体骨架基本信息 |
|
| 载体名称: | pXPR_047 |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | -- |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳动物表达，慢病毒，CRISPR |
| 筛选标记: | 嘌呤霉素 |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | GFP + sgRNA for GFP |
| 物种来源: | Synthetic |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pXPR_047 was a gift from David Root (Addgene plasmid # 107145 ; http://n2t.net/addgene:107145 ; RRID:Addgene_107145) |
买家导航
pXPR047载体基本信息
pXPR047载体图谱质粒图谱和多克隆位点信息
pXPR047载体简介
pXPR047载体序列
LOCUS pXPR_047 8543 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION measures Cas9 activity.
ACCESSION .
VERSION .
KEYWORDS pXPR_047
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8543)
TITLE David Root CRISPR plasmids
REFERENCE 2 (bases 1 to 8543)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..8543
/organism="synthetic DNA construct"
/mol_type="other DNA"
protein_bind 69..90
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 105..135
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 143..159
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 148..170
/label=M13/pUC Reverse
/note="In lacZ gene"
primer_bind 167..183
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 167..183
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind 202..222
/label=T3
/note="T3 promoter, forward primer"
promoter 204..222
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
promoter 250..476
/label=RSV promoter
/note="Rous sarcoma virus enhancer/promoter"
LTR 477..657
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
misc_feature 704..829
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 1322..1555
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
CDS 1740..1784
/codon_start=1
/product="antigenic peptide corresponding to amino acids
655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
et al., 2013)"
/label=gp41 peptide
/note="recognized by the 2H10 single-chain llama nanobody"
/translation="KNEQELLELDKWASL"
promoter 1962..2202
/label=U6 promoter
/note="RNA polymerase III promoter for human U6 snRNA"
primer_bind 1962..1982
/label=hU6-F
/note="Human U6 promoter, forward primer"
primer_bind 2133..2152
/label=LKO.1 5'
/note="Human U6 promoter, forward primer"
misc_feature 2364..2481
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
promoter 2540..3040
/label=hPGK promoter
/note="human phosphoglycerate kinase 1 promoter"
primer_bind 2959..2977
/label=hPGK-F
/note="Human PGK promoter, forward primer"
primer_bind complement(3052..3071)
/label=Puro-R
/note="Puromycin resistance gene, reverse primer. Also
called puro-variant-R"
primer_bind 3548..3568
/label=Puro-F
/note="Puromycin resistance gene, forward primer"
CDS 3688..3753
/codon_start=1
/product="2A peptide from foot-and-mouth disease virus
polyprotein"
/label=F2A
/note="Eukaryotic ribosomes fail to insert a peptide bond
between the Gly and Pro residues, yielding separate
polypeptides."
/translation="VKQTLNFDLLKLAGDVESNPGP"
CDS 3760..4476
/codon_start=1
/product="enhanced GFP"
/label=EGFP
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
CDS 3760..4476
/codon_start=1
/product="the original enhanced GFP (Yang et al., 1996)"
/label=EGFP
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
primer_bind complement(3805..3826)
/label=EGFP-N
/note="EGFP, reverse primer"
primer_bind complement(4066..4085)
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
primer_bind 4413..4434
/label=EGFP-C
/note="EGFP, forward primer"
misc_feature 4645..5233
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(4698..4718)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(5116..5127)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
LTR 5305..5538
/label=3' LTR (Delta-U3)
/note="self-inactivating 3' long terminal repeat (LTR) from
HIV-1"
polyA_signal 5616..5737
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(5653..5672)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 5707..5726
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
rep_origin 5777..5912
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 5839..5858
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
primer_bind complement(5932..5951)
/label=T7
/note="T7 promoter, forward primer"
promoter complement(5933..5951)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(5961..5978)
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind complement(5961..5977)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(5970..5992)
/label=M13/pUC Forward
/note="In lacZ gene"
rep_origin 6119..6574
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(6206..6225)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 6416..6437
/label=F1ori-F
/note="F1 origin, forward primer"
promoter 6600..6704
/gene="bla"
/label=AmpR promoter
CDS 6705..7565
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(6923..6942)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 7736..8324
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 8225..8244
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 8478..8495
/label=L4440
/note="L4440 vector, forward primer"
ORIGIN
1 ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc
61 aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt
121 ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat
181 gaccatgatt acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctg
241 caagcttaat gtagtcttat gcaatactct tgtagtcttg caacatggta acgatgagtt
301 agcaacatgc cttacaagga gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt
361 ggtacgatcg tgccttatta ggaaggcaac agacgggtct gacatggatt ggacgaacca
421 ctgaattgcc gcattgcaga gatattgtat ttaagtgcct agctcgatac ataaacgggt
481 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc
541 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg
601 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagtg
661 gcgcccgaac agggacttga aagcgaaagg gaaaccagag gagctctctc gacgcaggac
721 tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg cgactggtga gtacgccaaa
781 aattttgact agcggaggct agaaggagag agatgggtgc gagagcgtca gtattaagcg
841 ggggagaatt agatcgcgat gggaaaaaat tcggttaagg ccagggggaa agaaaaaata
901 taaattaaaa catatagtat gggcaagcag ggagctagaa cgattcgcag ttaatcctgg
961 cctgttagaa acatcagaag gctgtagaca aatactggga cagctacaac catcccttca
1021 gacaggatca gaagaactta gatcattata taatacagta gcaaccctct attgtgtgca
1081 tcaaaggata gagataaaag acaccaagga agctttagac aagatagagg aagagcaaaa
1141 caaaagtaag accaccgcac agcaagcggc cgctgatctt cagacctgga ggaggagata
1201 tgagggacaa ttggagaagt gaattatata aatataaagt agtaaaaatt gaaccattag
1261 gagtagcacc caccaaggca aagagaagag tggtgcagag agaaaaaaga gcagtgggaa
1321 taggagcttt gttccttggg ttcttgggag cagcaggaag cactatgggc gcagcgtcaa
1381 tgacgctgac ggtacaggcc agacaattat tgtctggtat agtgcagcag cagaacaatt
1441 tgctgagggc tattgaggcg caacagcatc tgttgcaact cacagtctgg ggcatcaagc
1501 agctccaggc aagaatcctg gctgtggaaa gatacctaaa ggatcaacag ctcctgggga
1561 tttggggttg ctctggaaaa ctcatttgca ccactgctgt gccttggaat gctagttgga
1621 gtaataaatc tctggaacag atttggaatc acacgacctg gatggagtgg gacagagaaa
1681 ttaacaatta cacaagctta atacactcct taattgaaga atcgcaaaac cagcaagaaa
1741 agaatgaaca agaattattg gaattagata aatgggcaag tttgtggaat tggtttaaca
1801 taacaaattg gctgtggtat ataaaattat tcataatgat agtaggaggc ttggtaggtt
1861 taagaatagt ttttgctgta ctttctatag tgaatagagt taggcaggga tattcaccat
1921 tatcgtttca gacccacctc ccaaccccga ggggacccag agagggccta tttcccatga
1981 ttccttcata tttgcatata cgatacaagg ctgttagaga gataattaga attaatttga
2041 ctgtaaacac aaagatatta gtacaaaata cgtgacgtag aaagtaataa tttcttgggt
2101 agtttgcagt tttaaaatta tgttttaaaa tggactatca tatgcttacc gtaacttgaa
2161 agtatttcga tttcttggct ttatatatct tgtggaaagg acgaaacacc ggtgaaccgc
2221 atcgagctga gtttaagagc tatgctggaa acagcatagc aagtttaaat aaggctagtc
2281 cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt ttgaattcta gatcttgaga
2341 caaatggcag tattcatcca caattttaaa agaaaagggg ggattggggg gtacagtgca
2401 ggggaaagaa tagtagacat aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa
2461 attacaaaaa ttcaaaattt tcgggtttat tacagggaca gcagagatcc actttggcgc
2521 cggctcgagg gggcccgggt tgcgcctttt ccaaggcagc cctgggtttg cgcagggacg
2581 cggctgctct gggcgtggtt ccgggaaacg cagcggcgcc gaccctgggt ctcgcacatt
2641 cttcacgtcc gttcgcagcg tcacccggat cttcgccgct acccttgtgg gccccccggc
2701 gacgcttcct gctccgcccc taagtcggga aggttccttg cggttcgcgg cgtgccggac
2761 gtgacaaacg gaagccgcac gtctcactag taccctcgca gacggacagc gccagggagc
2821 aatggcagcg cgccgaccgc gatgggctgt ggccaatagc ggctgctcag cagggcgcgc
2881 cgagagcagc ggccgggaag gggcggtgcg ggaggcgggg tgtggggcgg tagtgtgggc
2941 cctgttcctg cccgcgcggt gttccgcatt ctgcaagcct ccggagcgca cgtcggcagt
3001 cggctccctc gttgaccgaa tcaccgacct ctctccccag ggggatccac catgaccgag
3061 tacaagccca ccgtgcgcct cgccacccgc gacgacgtgc ccagggccgt ccgcaccctc
3121 gccgccgctt tcgccgacta ccccgccacc cgccacaccg tcgatcccga ccgccacatc
3181 gagcgggtca ccgagctgca agaactcttc ctcacccgcg tcgggctcga catcggcaag
3241 gtgtgggtcg ctgacgacgg cgccgcagtg gctgtctgga ccacacccga gagcgtcgaa
3301 gctggggcgg tgttcgccga gatcggccct cgcatggccg agttgagcgg ttcccggctg
3361 gccgctcagc aacagatgga aggcctcctg gctccacacc ggcccaagga gcccgcatgg
3421 ttcctggcca ccgtcggcgt ctctcccgac caccagggca agggtctggg cagcgccgtc
3481 gtgctccccg gagtggaggc tgccgagcgc gccggggtgc ccgccttcct ggagacctcc
3541 gcaccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc cgacgtggag
3601 gtgcccgaag gaccacgcac ctggtgcatg acccgcaagc ccggtgccct gcagggatct
3661 ggcgaagcca gacacaaaca gaaaattgtg aaacagactt tgaattttga ccttctcaag
3721 ttggcgggag acgtggagtc caacccaggg cccgctagca tggtgagcaa gggcgaggag
3781 ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag
3841 ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc
3901 atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctac
3961 ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc
4021 gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac
4081 aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag
4141 ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac
4201 agccacaacg tctatatcat ggccgacaag cagaagaacg gcatcaaggt gaacttcaag
4261 atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc
4321 cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccgcc
4381 ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc
4441 gccgggatca ctctcggcat ggacgagctg tacaagtccg gatcaggcgg aagcggcggg
4501 agtgggtcac acggctttcc tcccgaagtt gaggagcagg atgatggcac cctgccgatg
4561 tcctgcgctc aggaaagtgg catggacagg cacccagccg cgtgtgctag tgctagaatc
4621 aacgtgtaaa cgcgttaagt cgacaatcaa cctctggatt acaaaatttg tgaaagattg
4681 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct
4741 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg
4801 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact
4861 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc
4921 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc
4981 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa
5041 tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc
5101 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg
5161 gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg
5221 gccgcctccc cgcgtcgact ttaagaccaa tgacttacaa ggcagctgta gatcttagcc
5281 actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagatc
5341 tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg
5401 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
5461 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
5521 tgtggaaaat ctctagcagt acgtatagta gttcatgtca tcttattatt cagtatttat
5581 aacttgcaaa gaaatgaata tcagagagtg agaggaactt gtttattgca gcttataatg
5641 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
5701 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggctc tagctatccc
5761 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
5821 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt
5881 ccagaagtag tgaggaggct tttttggagg cctagggacg tacccaattc gccctatagt
5941 gagtcgtatt acgcgcgctc actggccgtc gttttacaac gtcgtgactg ggaaaaccct
6001 ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc
6061 gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatgggac
6121 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
6181 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
6241 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
6301 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca
6361 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
6421 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa
6481 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
6541 gcgaatttta acaaaatatt aacgcttaca atttaggtgg cacttttcgg ggaaatgtgc
6601 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
6661 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
6721 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
6781 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
6841 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
6901 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
6961 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
7021 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
7081 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
7141 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
7201 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
7261 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
7321 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
7381 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
7441 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
7501 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
7561 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
7621 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
7681 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
7741 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
7801 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
7861 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
7921 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
7981 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
8041 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
8101 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
8161 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
8221 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
8281 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
8341 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
8401 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
8461 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca
8521 aaccgcctct ccccgcgcgt tgg
//
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