| 质粒名称: | pYPQ141-ZmUbi-RZ-Lb |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 86197 |
| 存储实验室: | Yiping Qi |
|
载体骨架基本信息 |
|
| 载体名称: | pYPQ141 |
| 载体抗性: | 大观霉素 |
| 载体类型: | CRISPR |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | gRNA cloning site,Lb Cpf1 gRNA cloning site for ribozyme cleavage |
| 物种来源: | Lachnospiraceae bacterium ND2006 |
| 启动子 | Maize ubiquitin 1 |
| 克隆方法: | Gateway 克隆 |
| 5' 测序引物 | ttcccagtcacgacgttgtaaaac |
| 3' 测序引物 | catggtcatagctgtttcctg |
|
文献引用方法 |
|
| 材料和方法部分: | pYPQ141-ZmUbi-RZ-Lb was a gift from Yiping Qi (Addgene plasmid # 86197 ; http://n2t.net/addgene:86197 ; RRID:Addgene_86197) |
| 参考文献部分: | A CRISPR-Cpf1 system for efficient genome editing and transcriptional repression in plants. Tang X, Lowder LG, Zhang T, Malzahn AA, Zheng X, Voytas DF, Zhong Z, Chen Y, Ren Q, Li Q, Kirkland ER, Zhang Y, Qi Y. Nat Plants. 2017 Feb 17;3:17018. doi: 10.1038/nplants.2017.18. 10.1038/nplants.2017.18 PubMed 28211909 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pYPQ141-ZmUbi-RZ-Lb 4991 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION LbCpf1 Gateway gRNA entry plasmid using Zea mays Ubi promoter and
ribozyme processing.
ACCESSION .
VERSION .
KEYWORDS pYPQ141-ZmUbi-RZ-Lb
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4991)
AUTHORS Tang X, Lowder LG, Zhang T, Malzahn AA, Zheng X, Voytas DF, Zhong Z,
Chen Y, Ren Q, Li Q, Kirkland ER, Zhang Y, Qi Y
TITLE A CRISPR-Cpf1 system for efficient genome editing and
transcriptional repression in plants.
JOURNAL Nat Plants. 2017 Feb 17;3:17018. doi: 10.1038/nplants.2017.18.
PUBMED 28211909
REFERENCE 2 (bases 1 to 4991)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..4991
/organism="synthetic DNA construct"
/mol_type="other DNA"
protein_bind complement(1217..1316)
/gene="mutant version of attL"
/label=attL2
/bound_moiety="LR Clonase(TM)"
/note="recombination site for the Gateway(R) LR reaction"
promoter complement(1334..1352)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
promoter complement(1369..1387)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(1392..1408)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(1392..1408)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
CDS 1839..2630
/codon_start=1
/gene="aadA"
/product="aminoglycoside adenylyltransferase (Murphy,
1985)"
/label=SmR
/note="confers resistance to spectinomycin and
streptomycin"
/translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
rep_origin 2723..3311
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 3212..3231
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 3465..3482
/label=L4440
/note="L4440 vector, forward primer"
terminator 3641..3668
/label=rrnB T2 terminator
/note="transcription terminator T2 from the E. coli rrnB
gene"
terminator 3760..3846
/gene="Escherichia coli rrnB"
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
primer_bind 3895..3917
/label=M13/pUC Forward
/note="In lacZ gene"
primer_bind 3909..3926
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind 3910..3926
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
protein_bind 3941..4036
/gene="mutant version of attL"
/label=attL5
/bound_moiety="LR Clonase(TM)"
/note="recombination site for the Gateway(R) LR reaction"
ORIGIN
1 cctccgcttc aaggtacgcc gctcgtcctc cccccccccc cctctctacc ttctctagat
61 cggcgttccg gtccatggtt agggcccggt agttctactt ctgttcatgt ttgtgttaga
121 tccgtgtttg tgttagatcc gtgctgctag cgttcgtaca cggatgcgac ctgtacgtca
181 gacacgttct gattgctaac ttgccagtgt ttctctttgg ggaatcctgg gatggctcta
241 gccgttccgc agacgggatc gatttcatga ttttttttgt ttcgttgcat agggtttggt
301 ttgccctttt cctttatttc aatatatgcc gtgcacttgt ttgtcgggtc atcttttcat
361 gctttttttt gtcttggttg tgatgatgtg gtctggttgg gcggtcgttc tagatcggag
421 tagaattaat tctgtttcaa actacctggt ggatttatta attttggatc tgtatgtgtg
481 tgccatacat attcatagtt acgaattgaa gatgatggat ggaaatatcg atctaggata
541 ggtatacatg ttgatgcggg ttttactgat gcatatacag agatgctttt tgttcgcttg
601 gttgtgatga tgtggtgtgg ttgggcggtc gttcattcgt tctagatcgg agtagaatac
661 tgtttcaaac tacctggtgt atttattaat tttggaactg tatgtgtgtg tcatacatct
721 tcatagttac gagtttaaga tggatggaaa tatcgatcta ggataggtat acatgttgat
781 gtgggtttta ctgatgcata tacatgatgg catatgcagc atctattcat atgctctaac
841 cttgagtacc tatctattat aataaacaag tatgttttat aattattttg atcttgatat
901 acttggatga tggcatatgc agcagctata tgtggatttt tttagccctg ccttcatacg
961 ctatttattt gcttggtact gtttcttttg tcgatgctca ccctgttgtt tggtgttact
1021 tctgcaggga tccaaattac tgatgagtcc gtgaggacga aacgagtaag ctcgtctaat
1081 ttctactaag tgtagatgag acggagctca gtctgaccgc ggcgtctctg gccggcatgg
1141 tcccagcctc ctcgctggcg ccggctgggc aacatgcttc ggcatggcga atgggacggt
1201 acccggccgg aattcgaccc agctttcttg tacaaagttg gcattataaa aaataattgc
1261 tcatcaattt gttgcaacga acaggtcact atcagtcaaa ataaaatcat tatttgccat
1321 ccagctgata tcccctatag tgagtcgtat tacatggtca tagcatcccc tatagtgagt
1381 cgtattacat ggtcatagct gtttcctggc agctctggcc cgtgtctcaa aatctctgat
1441 gttacattgc acaagataaa aatatatcat catgcctcct ctggaccagc caggacagaa
1501 atgcctcgac ttcgctgcta cccaaggttg ccgggtgacg cacaccgtgg aaacggatga
1561 aggcacgaac ccagtggaca taagcctgtt cggttcgtaa gctgtaatgc aagtagcgta
1621 tgcgctcacg caactggtcc agaaccttga ccgaacgcag cggtggtaac ggcgcagtgg
1681 cggttttcat ggcttgttat gactgttttt ttggggtaca gtctatgcct cgggcatcca
1741 agcagcaagc gcgttacgcc gtgggtcgat gtttgatgtt atggagcagc aacgatgtta
1801 cgcagcaggg cagtcgccct aaaacaaagt taaacattat gagggaagcg gtgatcgccg
1861 aagtatcgac tcaactatca gaggtagttg gcgtcatcga gcgccatctc gaaccgacgt
1921 tgctggccgt acatttgtac ggctccgcag tggatggcgg cctgaagcca cacagtgata
1981 ttgatttgct ggttacggtg accgtaaggc ttgatgaaac aacgcggcga gctttgatca
2041 acgacctttt ggaaacttcg gcttcccctg gagagagcga gattctccgc gctgtagaag
2101 tcaccattgt tgtgcacgac gacatcattc cgtggcgtta tccagctaag cgcgaactgc
2161 aatttggaga atggcagcgc aatgacattc ttgcaggtat cttcgagcca gccacgatcg
2221 acattgatct ggctatcttg ctgacaaaag caagagaaca tagcgttgcc ttggtaggtc
2281 cagcggcgga ggaactcttt gatccggttc ctgaacagga tctatttgag gcgctaaatg
2341 aaaccttaac gctatggaac tcgccgcccg actgggctgg cgatgagcga aatgtagtgc
2401 ttacgttgtc ccgcatttgg tacagcgcag taaccggcaa aatcgcgccg aaggatgtcg
2461 ctgccgactg ggcaatggag cgcctgccgg cccagtatca gcccgtcata cttgaagcta
2521 gacaggctta tcttggacaa gaagaagatc gcttggcctc gcgcgcagat cagttggaag
2581 aatttgtcca ctacgtgaaa ggcgagatca ccaaggtagt cggcaaataa ccctcgagcc
2641 acccatgacc aaaatccctt aacgtgagtt acgcgtcgtt ccactgagcg tcagaccccg
2701 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
2761 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
2821 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
2881 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
2941 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
3001 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
3061 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
3121 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
3181 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
3241 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
3301 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
3361 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
3421 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
3481 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
3541 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
3601 atacgcgtac cgcgagccag gaagagtttg tagaaacgca aaaaggccat ccgtcaggat
3661 ggccttctgc ttagtttgat gcctggcagt ttatggcggg cgtcctgccc gccaccctcc
3721 gggccgttgc ttcacaacgt tcaaatccgc tcccggcgga tttgtcctac tcaggagagc
3781 gttcaccgac aaacaacaga taaaacgaaa ggcccagtct tccgactgag cctttcgttt
3841 tatttgatgc ctggcagttc cctactctcg cgttaacgct tgcatggatg ttttcccagt
3901 cacgacgttg taaaacgacg gccagtctta agctcgggcc caataatgat tttattttga
3961 ctgatagtga cctgttcgtt gcaacaaatt gatgagcaat gcttttttat aatgccaact
4021 ttgtatacaa aagttgcccc atggcgttcc ctctagagag ataatgagca ttgcatgtct
4081 aagttataaa aaattaccac atattttttt tgtcacactt gtttgaagtg cagtttatct
4141 atctttatac atatatttaa actttactct acgaataata taatctatag tactacaata
4201 atatcagtgt tttagagaat catataaatg aacagttaga catggtctaa aggacaattg
4261 agtattttga caacaggact ctacagtttt atctttttag tgtgcatgtg ttctcctttt
4321 tttttgcaaa tagcttcacc tatataatac ttcatccatt ttattagtac atccatttag
4381 ggtttagggt taatggtttt tatagactaa tttttttagt acatctattt tattctattt
4441 tagcctctaa attaagaaaa ctaaaactct attttagttt ttttatttaa taatttagat
4501 ataaaataga ataaaataaa gtgactaaaa attaaacaaa taccctttaa gaaattaaaa
4561 aaactaagga aacatttttc ttgtttcgag tagataatgc cagcctgtta aacgccgtcg
4621 acgagtctaa cggacaccaa ccagcgaacc agcagcgtcg cgtcgggcca agcgaagcag
4681 acggcacggc atctctgtcg ctgcctctgg acccctctcg agagttccgc tccaccgttg
4741 gacttgctcc gctgtcggca tccagaaatt gcgtggcgga gcggcagacg tgagccggca
4801 cggcaggcgg cctcctcctc ctctcacggc accggcagct acgggggatt cctttcccac
4861 cgctccttcg ctttcccttc ctcgcccgcc gtaataaata gacaccccct ccacaccctc
4921 tttccccaac ctcgtgttgt tcggagcgca cacacacaca accagatctc ccccaaatcc
4981 acccgtcggc a
//
