| 质粒名称: | pc3XB-ZF102 |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 13236 |
| 存储实验室: | Daniel Voytas |
|
载体骨架基本信息 |
|
| 载体名称: | pc3XB |
| 载体出品公司: | Modified Invitrogen pcDNA3 |
| 空载体大小: | 5400 bp |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | 新霉素（G418） |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | XL1 Blue |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | ZF102 |
| 物种来源: | -- |
| 插入大小: | 90 bp |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子: | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | XbaI (未破坏) |
| 3’克隆位点: | BamHI (未破坏) |
| 5’测序引物 | T7 |
| 3’测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pc3XB-ZF102 was a gift from Daniel Voytas (Addgene plasmid # 13236 ; http://n2t.net/addgene:13236 ; RRID:Addgene_13236) |
买家导航
pc3XBZF102载体基本信息
pc3XBZF102载体图谱质粒图谱和多克隆位点信息
pc3XBZF102载体简介
pc3XBZF102载体序列
LOCUS pc3XB-ZF102 5466 bp ds-DNA circular SYN 13-MAY-2021
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pc3XB-ZF102
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5466)
TITLE Zinc Finger Consortium Modular Assembly Kit v1.0B
REFERENCE 2 (bases 1 to 5466)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..5466
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind complement(44..63)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
enhancer 235..614
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 615..818
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 769..789
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind 863..882
/label=T7
/note="T7 promoter, forward primer"
promoter 863..881
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(1056..1073)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 1062..1286
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 1332..1760
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(1419..1438)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 1629..1650
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind complement(1769..1789)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 1774..2103
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 1954..2089
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 2016..2035
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
CDS 2174..2968
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(2228..2247)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 2838..2857
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
polyA_signal 3142..3263
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(3179..3198)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 3233..3252
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind complement(3312..3328)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(3312..3328)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(3325..3347)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 3336..3352
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(3360..3390)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 3405..3426
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(3543..3560)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(3714..4299)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(3794..3813)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(4470..5330)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 5093..5112
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(5331..5435)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
901 gtttaaacgg gccctctaga cccggggaga agccttacaa atgcccagaa tgtggaaaga
961 gttttagcag gaatgatgca ctcacagaac accagagaac acataccggt ggatcctctg
1021 cacagtagta agcttaagtt taaaccgctg atcagcctcg actgtgcctt ctagttgcca
1081 gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac
1141 tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat
1201 tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca
1261 tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctctag
1321 ggggtatccc cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
1381 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
1441 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
1501 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
1561 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
1621 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
1681 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
1741 acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc
1801 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg
1861 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
1921 tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
1981 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
2041 tctgcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc
2101 aaaaagctcc cggccgggag cttgtatatc cattttcgga tctgatcaag agacaggatg
2161 aggatcgttt cgcatgattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt
2221 ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt
2281 gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc
2341 cctgaatgaa ctgcaggacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc
2401 ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga
2461 agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat
2521 ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca
2581 agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga
2641 tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc
2701 gcgcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat
2761 catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga
2821 ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg
2881 ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt
2941 ctatcgcctt cttgacgagt tcttctgagc gggactctgg ggttcgaaat gaccgaccaa
3001 gcgacgccca acctgccatc acgagatttc gattccaccg ccgccttcta tgaaaggttg
3061 ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg
3121 ctggagttct tcgcccaccc caacttgttt attgcagctt ataatggtta caaataaagc
3181 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
3241 tccaaactca tcaatgtatc ttatcatgtc tgtataccgt cgacctctag ctagagcttg
3301 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
3361 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
3421 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
3481 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
3541 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
3601 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
3661 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
3721 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
3781 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
3841 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
3901 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
3961 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
4021 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
4081 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
4141 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
4201 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tttttttgtt
4261 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
4321 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
4381 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
4441 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
4501 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
4561 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
4621 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
4681 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
4741 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
4801 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
4861 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
4921 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
4981 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
5041 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
5101 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
5161 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
5221 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
5281 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
5341 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
5401 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
5461 gacgtc
//
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