| 质粒名称: | pcDNA-3xflagGREB1a |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 112221 |
| 存储实验室: | Craig Burd |
|
载体骨架基本信息 |
|
| 载体名称: | pcDNA3.1 + hygro |
| 载体出品公司: | -- |
| 空载体大小: | -- |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | 潮霉素 |
| 载体抗性: | 氨苄霉素100 μg/mL |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | GREB1a |
| 物种来源: | H. sapiens (human) |
| 插入大小: | 5846 bp |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子: | -- |
| 标签/融合蛋白: | 3x标志（插入件上的N端子） |
| 克隆方法: | Gibson 克隆 |
| 5’克隆位点: | -- |
| 3’克隆位点: | -- |
| 5’测序引物 | T7 or CMV |
| 3’测序引物 | BGH R |
|
文献引用方法 |
|
| 材料和方法部分: | pcDNA-3xflagGREB1a was a gift from Craig Burd (Addgene plasmid # 112221 ; http://n2t.net/addgene:112221 ; RRID:Addgene_112221) |
| 参考文献部分: | GREB1 isoforms regulate proliferation independent of ERalpha co-regulator activities in breast cancer. Haines CN, Braunreiter KM, Mo XM, Burd CJ. Endocr Relat Cancer. 2018 Jul;25(7):735-746. doi: 10.1530/ERC-17-0496. Epub 2018 Apr 25. 10.1530/ERC-17-0496 PubMed 29695586 |
买家导航
pcDNA3xflagGREB1a载体基本信息
pcDNA3xflagGREB1a载体图谱质粒图谱和多克隆位点信息
pcDNA3xflagGREB1a载体简介
pcDNA3xflagGREB1a载体序列
LOCUS pcDNA-3xflagGREB1a 11457 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION mammalian expression of flag tagged GREB1a.
ACCESSION .
VERSION .
KEYWORDS pcDNA-3xflagGREB1a
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 11457)
AUTHORS Haines CN, Braunreiter KM, Mo XM, Burd CJ
TITLE GREB1 isoforms regulate proliferation independent of ERalpha
co-regulator activities in breast cancer.
JOURNAL Endocr Relat Cancer. 2018 Jul;25(7):735-746. doi:
10.1530/ERC-17-0496. Epub 2018 Apr 25.
PUBMED 29695586
REFERENCE 2 (bases 1 to 11457)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..11457
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 1..380
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 381..584
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 535..555
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind 629..648
/label=T7
/note="T7 promoter, forward primer"
promoter 629..647
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
regulatory 662..671
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 671..736
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
primer_bind complement(6645..6662)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 6651..6875
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 6921..7349
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(7008..7027)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 7218..7239
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind complement(7358..7378)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 7363..7692
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 7543..7678
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 7605..7624
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
CDS 7741..8766
/codon_start=1
/gene="aph(4)-Ia"
/product="aminoglycoside phosphotransferase from E. coli"
/label=HygR
/note="confers resistance to hygromycin"
/translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
E"
polyA_signal 8896..9017
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(8933..8952)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 8987..9006
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind complement(9066..9082)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(9066..9082)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(9079..9101)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 9090..9106
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(9114..9144)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 9159..9180
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(9297..9314)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(9468..10056)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(9548..9567)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(10227..11087)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 10850..10869
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(11088..11192)
/gene="bla"
/label=AmpR promoter
primer_bind complement(11267..11286)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
ORIGIN
1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag
601 aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag acccaagctg
661 ggccaccatg gactacaaag accatgacgg tgattataaa gatcatgata tcgattacaa
721 ggatgacgat gacaagctag cgtttaaact taagcttggt accatgggaa attcttacgc
781 tggacagctg aagacgacac gctttgaaga ggtcttgcac aattccatcg aggcatccct
841 gcggtccaac aacctggtgc ccaggcccat cttttcccag ctgtacctgg aagctgagca
901 gcagcttgcc gctctagaag gtggtagccg agtggacaat gaggaagagg aagaagaggg
961 agaaggaggg ctggaaacaa atggcccccc aaaccctttc cagctgcacc ctctgcctga
1021 aggatgctgt accacagacg ggttttgcca ggccgggaag gacctgcgcc ttgtctccat
1081 ttccaacgag cccatggatg tccctgcggg ctttctcctc gtgggggtca agtcccccag
1141 cctgccggac catctcctgg tgtgcgccgt tgacaagagg ttcttgccag atgacaatgg
1201 ccacaatgct cttcttggtt tctctgggaa ttgtgttggc tgtggaaaga aaggcttctg
1261 ttacttcacg gaattctcca atcatataaa tctgaaactg accactcaac ccaagaagca
1321 gaaacacttg aagtattacc tggtccgtaa tgcacaaggg actctaacca aaggaccttt
1381 aatctgttgg aaaggctcag agtttagaag ccggcagatc cccgccagta cttgttccag
1441 ttccctcttc ccagccctgg agagcacggc tgccttcccc agcgagcccg ttcctgggac
1501 gaaccccagc atcctgatgg gagctcagca ggcaggacca gcttctgatc acccctcact
1561 aaacgcagca atgggtccgg ctgttttcaa cggcaaagat tccccgaagt gccaacaact
1621 ggcaaagaat aacctgttgg ccctgccgcg accatcggct ttaggtatct tgtcaaactc
1681 cgggcccccc aaaaaacgcc acaaagggtg gtctccagaa tctccatcag ctccagatgg
1741 tggctgcccc caaggtggtg ggaacagagc taagtatgag agcgcaggca tgtcctgcgt
1801 gccgcaggtt ggcttggtgg gaccagcttc agtcaccttt ccagtggtgg cctctggaga
1861 accagtgtct gttcctgaca acttgctgaa aatatgcaag gccaagccag tgatatttaa
1921 aggccatggg aacttccctt acctctgtgg gaacctgaat gacgtcgtgg tcagccccct
1981 cttgtacacg tgctaccaga attcccagtc tgtctcacgg gcatacgagc agtacggcgc
2041 ctctgccatc cagcccatct ccgaggagat gcagctcctg cttaccgtct actacctggt
2101 ccagctggcc gcggaccagg tgcccttgat ggaggacctg gagcagatct tcctgcgctc
2161 ttggcgcgag tcgcacctga ccgagatccg gcagtaccag caggcgccgc cgcagccctt
2221 cccgcccgcg cccagcgccg cggcacccgt gacctccgcg cagctgccct ggctggccag
2281 cctggccgcc agctcctgca acgacagcgt gcacgtcatc gagtgtgctt actccctggc
2341 cgagggcctc tccgagatgt tccggctgtt ggtcgagggc aagcttgcca agaccaacta
2401 cgtggtcatc atctgcgcct gccgcagcgc ggccatcgac tcctgcatcg ccgtcaccgg
2461 aaaataccaa gcccggattc tttccgagag ccttctcact cctgcggagt accagaagga
2521 agtcaattac gagctggtta cggggaaggt agactcgctg ggggccttct ttagcaccct
2581 ctgtccagag ggtgacattg acattttgct ggacaaattt caccaggaaa atcaaggcca
2641 tatttcttcc tcactcgctg cctcttctgt cactaaagca gcatccctgg atgtcagtgg
2701 gacaccggtg tgcacaagtt acaatctgga gccacacagc atccggccct tccagctggc
2761 agtagcgcag aagctcctct cccatgtgtg ttccattgcg gattccagca cccaaaatct
2821 ggacctggga tcctttgaga aggtggactt tctcatttgc attcccccct cagaagtgac
2881 ctaccagcag actctgctcc acgtgtggca ttcaggggtt ttgctggagc ttggtctgaa
2941 gaaagagcac atgacgaagc agagggtgga acagtatgtt ctgaagctag acacggaggc
3001 acagacaaaa tttaaggctt ttctgcaaaa ctccttccag aacccgcata cactttttgt
3061 cctaatccat gaccatgcgc actgggatct tgtgagtagc actgttcata acctctattc
3121 tcaaagtgac ccgtcggtgg gattggtgga ccgattgctc aactgcaggg aggtgaagga
3181 ggcccccaac attgtgacac ttcacgtgac ctccttcccg tatgcactgc agacacagca
3241 caccctcatc agcccctaca acgagatcca ctggcctgcc tcctgcagta atggagtgga
3301 cttatatcat gaaaataaga agtacttcgg gctgtcggag tttattgaat ccaccctttc
3361 aggacacagc ctccccttgc tcagatacga tagctccttt gaggccatgg tcactgcatt
3421 aggaaaaagg ttcccccgcc tgcacagcgc ggtgatcagg acctttgttc tcgtgcagca
3481 ctacgcggcc gccctgatgg ccgtaagcgg cctcccgcag atgaagaact acacgtcggt
3541 ggagacgctg gagatcacgc agaacctcct caactccccg aagcagtgcc cctgcggcca
3601 cgggctcatg gtcctgctgc gggtgccctg ttcgcccctg gcggtggtgg cctatgagcg
3661 gctggcccac gtgcgggccc ggctggcgct ggaggagcac tttgagatca tcctgggcag
3721 tcccagctca ggcgtcaccg tggggaagca cttcgtaaag cagctcagga tgtggcagaa
3781 aatcgaggat gtggagtgga gaccccagac ttacttggag ctggagggtc tgccttgcat
3841 cctgatcttc agtgggatgg acccgcatgg ggagtccttg ccgaggtctt tgaggtactg
3901 tgacctgcga ttgataaact cctcctgctt ggtgagaaca gccttggagc aggagctggg
3961 cctggctgcc tactttgtga gcaacgaggt tcccttggag aagggggcta ggaacgaggc
4021 cttggagagt gatgctgaga agctgagcag cacagacaac gaggatgagg agctggggac
4081 agaaggctct acctcggaga agagaagccc catgaaaagg gagaggtccc gctcccacga
4141 ctcagcatcc tcatccctct cctccaaggc ttccggttca gcgctcggtg gcgagtcctc
4201 ggctcagccc acagcactcc cccagggaga gcatgccagg tcgccccagc cccgtggccc
4261 cgcagaggag ggcagagccc ctggtgagaa acagaggccc cgggcaagtc aggggccacc
4321 ctcggccatc agcaggcaca gtcccgggcc gacgccccag cccgactgta gcctcaggac
4381 cggccagagg agcgtccagg tgtcggtcac ctcgtcgtgc tcccagctgt cctcctcctc
4441 gggctcatcc tcctcatccg tggcgcctgc tgccggcacg tgggtcctgc aggcctccca
4501 gtgctccttg accaaggcct gccgccagcc acccattgtc ttcttgccca agctcgtgta
4561 cgacatggtt gtgtccactg acagcagtgg cctgcccaag gccgcctccc tcctgccctc
4621 cccctcggtc atgtgggcca gctctttccg ccccctgctc agcaagacca tgacatccac
4681 cgagcagtcc ctctactacc ggcagtggac ggtgccccgg cccagccaca tggactacgg
4741 caaccgggcc gagggccgcg tggacggctt ccacccccgc aggctgctgc tcagcggccc
4801 ccctcagatc gggaagacag gtgcctacct gcagttcctc agtgtcctgt ccaggatgct
4861 tgttcggctc acagaagtgg atgtctatga cgaggaggag atcaatatca acctcagaga
4921 agaatctgac tggcattatc tccagcttag cgacccctgg ccagacctgg agctgttcaa
4981 gaagttgccc tttgactaca tcattcacga cccgaagtat gaagatgcca gcctgatttg
5041 ttcgcactat cagggtataa agagtgaaga cagagggatg tcccggaagc cggaggacct
5101 ttatgtgcgg cgtcagacgg cacggatgag actgtccaag tacgcagcgt acaacactta
5161 ccaccactgt gagcagtgcc accagtacat gggcttccac ccccgctacc agctgtatga
5221 gtccaccctg cacgcctttg ccttctctta ctccatgcta ggagaggaga tccagctgca
5281 cttcatcatc cccaagtcca aggagcacca ctttgtcttc agccaacctg gaggccagct
5341 ggagagcatg cgactacccc tcgtgacaga caagagccat gaatatataa aaagtccgac
5401 attcactcca accaccggcc gtcacgaaca tgggctcttt aatctgtacc acgcaatgga
5461 cggtgccagc catttgcacg tgctggttgt caaggaatac gagatggcaa tttataagaa
5521 atattggccc aaccacatca tgctggtgct ccccagtatc ttcaacagtg ctggagttgg
5581 tgctgctcat ttcctcatca aggagctgtc ctaccataac ctggagctcg agcggaaccg
5641 gcaggaggag ctgggaatca agccgcagga catctggcct ttcattgtga tctctgatga
5701 ctcctgcgtg atgtggaacg tggtggatgt caactctgct ggggagagaa gcagggagtt
5761 ctcctggtcg gaaaggaacg tgtctttgaa gcacatcatg cagcacatcg aggcggcccc
5821 cgacatcatg cactacgccc tgctgggcct gcggaagtgg tccagcaaga cccgggccag
5881 cgaggtgcaa gagcccttct cccgctgcca cgtgcacaac ttcatcatcc tgaacgtgga
5941 cctgacccag aacgtgcagt acaaccagaa ccggttcctg tgtgacgatg tagacttcaa
6001 cctgcgggtg cacagcgccg gcctcctgct ctgccggttc aaccgcttca gcgtgatgaa
6061 gaagcagatc gtggtgggcg gccacaggtc cttccacatc acatccaagg tgtctgataa
6121 ctctgccgcg gtcgtgccgg cccagtacat ctgtgccccg gacagcaagc acacgttcct
6181 cgcagcgccc gcccagctcc tgctggagaa gttcctgcag caccacagcc acctcttctt
6241 cccgctgtcc ctgaagaacc atgaccaccc agtgctgtct gtcgactgtt acctgaacct
6301 gggatctcag atttctgttt gctatgtgag ctccaggccc cactctttaa acatcagctg
6361 ctcggacttg ctgttcagtg ggctgctgct gtacctctgt gactcttttg tgggagctag
6421 ctttttgaaa aagtttcatt ttctgaaagg tgcgacgttg tgtgtcatct gtcaggaccg
6481 gagctcactg cgccagacgg tcgtccgcct ggagctcgag gacgagtggc agttccggct
6541 gcgcgatgag ttccagaccg ccaatgccag ggaagaccgg ccgctctttt ttctgacggg
6601 acgacacatc tgaactagag ggcccgttta aacccgctga tcagcctcga ctgtgccttc
6661 tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc
6721 cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg
6781 tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa
6841 tagcaggcat gctggggatg cggtgggctc tatggcttct gaggcggaaa gaaccagctg
6901 gggctctagg gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt
6961 ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt
7021 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct
7081 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg
7141 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga
7201 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc
7261 ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga
7321 gctgatttaa caaaaattta acgcgaatta attctgtgga atgtgtgtca gttagggtgt
7381 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
7441 gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
7501 ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg
7561 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
7621 gaggccgcct ctgcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
7681 ggcttttgca aaaagctccc gggagcttgt atatccattt tcggatctga tcagcacgtg
7741 atgaaaaagc ctgaactcac cgcgacgtct gtcgagaagt ttctgatcga aaagttcgac
7801 agcgtctccg acctgatgca gctctcggag ggcgaagaat ctcgtgcttt cagcttcgat
7861 gtaggagggc gtggatatgt cctgcgggta aatagctgcg ccgatggttt ctacaaagat
7921 cgttatgttt atcggcactt tgcatcggcc gcgctcccga ttccggaagt gcttgacatt
7981 ggggaattca gcgagagcct gacctattgc atctcccgcc gtgcacaggg tgtcacgttg
8041 caagacctgc ctgaaaccga actgcccgct gttctgcagc cggtcgcgga ggccatggat
8101 gcgatcgctg cggccgatct tagccagacg agcgggttcg gcccattcgg accgcaagga
8161 atcggtcaat acactacatg gcgtgatttc atatgcgcga ttgctgatcc ccatgtgtat
8221 cactggcaaa ctgtgatgga cgacaccgtc agtgcgtccg tcgcgcaggc tctcgatgag
8281 ctgatgcttt gggccgagga ctgccccgaa gtccggcacc tcgtgcacgc ggatttcggc
8341 tccaacaatg tcctgacgga caatggccgc ataacagcgg tcattgactg gagcgaggcg
8401 atgttcgggg attcccaata cgaggtcgcc aacatcttct tctggaggcc gtggttggct
8461 tgtatggagc agcagacgcg ctacttcgag cggaggcatc cggagcttgc aggatcgccg
8521 cggctccggg cgtatatgct ccgcattggt cttgaccaac tctatcagag cttggttgac
8581 ggcaatttcg atgatgcagc ttgggcgcag ggtcgatgcg acgcaatcgt ccgatccgga
8641 gccgggactg tcgggcgtac acaaatcgcc cgcagaagcg cggccgtctg gaccgatggc
8701 tgtgtagaag tactcgccga tagtggaaac cgacgcccca gcactcgtcc gagggcaaag
8761 gaatagcacg tgctacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc
8821 ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag
8881 ttcttcgccc accccaactt gtttattgca gcttataatg gttacaaata aagcaatagc
8941 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
9001 ctcatcaatg tatcttatca tgtctgtata ccgtcgacct ctagctagag cttggcgtaa
9061 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
9121 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
9181 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
9241 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
9301 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
9361 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
9421 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
9481 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
9541 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
9601 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
9661 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
9721 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
9781 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
9841 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
9901 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
9961 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
10021 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
10081 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
10141 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
10201 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
10261 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
10321 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
10381 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
10441 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
10501 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
10561 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
10621 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
10681 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
10741 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
10801 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
10861 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
10921 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
10981 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
11041 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
11101 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
11161 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
11221 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
11281 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
11341 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
11401 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgtt
//
