| 质粒名称: | phCMV-GALV-MTR |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 163612 |
| 存储实验室: | Daniel Hodson |
|
载体骨架基本信息 |
|
| 载体名称: | phCMV-ECO-ENV |
| 载体出品公司: | Addgene 15802 |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 4764 bp |
| 完整质粒大小: | 6825 bp |
| 载体类型: | 哺乳细胞表达 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | GaLV MTR envelope |
| 插入大小: | 2061 bp |
| 启动子 | CMV |
| 克隆方法: | Gibson 克隆 |
| 5' 测序引物 | CTGGTCATCATCCTGCCTTT |
| 3' 测序引物 | TTTTGGCAGAGGGAAAAAGA |
|
文献引用方法 |
|
| 材料和方法部分: | phCMV-GALV-MTR was a gift from Daniel Hodson (Addgene plasmid # 163612 ; http://n2t.net/addgene:163612 ; RRID:Addgene_163612) |
| 参考文献部分: | Genetic modification of primary human B cells to model high-grade lymphoma. Caeser R, Di Re M, Krupka JA, Gao J, Lara-Chica M, Dias JML, Cooke SL, Fenner R, Usheva Z, Runge HFP, Beer PA, Eldaly H, Pak HK, Park CS, Vassiliou GS, Huntly BJP, Mupo A, Bashford-Rogers RJM, Hodson DJ. Nat Commun. 2019 Oct 4;10(1):4543. doi: 10.1038/s41467-019-12494-x. 10.1038/s41467-019-12494-x PubMed 31586074 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS phCMV-GALV-MTR 6825 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Retro and Lentiviral transduction of primary human germinal center B
cells.
ACCESSION .
VERSION .
KEYWORDS phCMV-GALV-MTR
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6825)
AUTHORS Caeser R, Di Re M, Krupka JA, Gao J, Lara-Chica M, Dias JML, Cooke
SL, Fenner R, Usheva Z, Runge HFP, Beer PA, Eldaly H, Pak HK, Park
CS, Vassiliou GS, Huntly BJP, Mupo A, Bashford-Rogers RJM, Hodson DJ
TITLE Genetic modification of primary human B cells to model high-grade
lymphoma.
JOURNAL Nat Commun. 2019 Oct 4;10(1):4543. doi: 10.1038/s41467-019-12494-x.
PUBMED 31586074
REFERENCE 2 (bases 1 to 6825)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..6825
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 86..465
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 466..669
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 620..640
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind 666..690
/label=LNCX
/note="Human CMV promoter, forward primer"
intron 781..1353
/label=-beta-globin intron
/note="intron from rabbit beta-globin gene"
primer_bind complement(3519..3538)
/label=Bglob-pA-R
/note="Rabbit beta-globin polyA region, reverse primer"
polyA_signal 3584..3639
/label=-beta-globin poly(A) signal
/note="rabbit beta-globin polyadenylation signal (Gil and
Proudfoot, 1987)"
primer_bind complement(3638..3657)
/label=rbglobpA-R
/note="Rabbit beta-globin polyA, reverse primer. Also
called rb-glob-pA-term-R"
primer_bind complement(3997..4013)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(3997..4013)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(4010..4032)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 4021..4037
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(4045..4075)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 4090..4111
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(4228..4245)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(4399..4987)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(4479..4498)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(5158..6018)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 5781..5800
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(6019..6123)
/gene="bla"
/label=AmpR promoter
rep_origin complement(6149..6604)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(6286..6307)
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind 6498..6517
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 6731..6753
/label=M13/pUC Forward
/note="In lacZ gene"
primer_bind 6745..6762
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind 6746..6762
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 6772..6791
/label=T7
/note="T7 promoter, forward primer"
promoter 6772..6790
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
1 agagagcttg gcccattgca tacgttgtat ccatatcata atatgtacat ttatattggc
61 tcatgtccaa cattaccgcc atgttgacat tgattattga ctagttatta atagtaatca
121 attacggggt cattagttca tagcccatat atggagttcc gcgttacata acttacggta
181 aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat
241 gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg
301 taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac
361 gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt
421 cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg
481 cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc
541 attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt
601 aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata
661 agcagagctc gtttagtgaa ccgtcagatc gcctggagac gccatccacg ctgttttgac
721 ctccatagaa gacaccggga ccgatccagc ctccggtcga ccgatcctga gaacttcagg
781 gtgagtttgg ggacccttga ttgttctttc tttttcgcta ttgtaaaatt catgttatat
841 ggagggggca aagttttcag ggtgttgttt agaatgggaa gatgtccctt gtatcaccat
901 ggaccctcat gataattttg tttctttcac tttctactct gttgacaacc attgtctcct
961 cttattttct tttcattttc tgtaactttt tcgttaaact ttagcttgca tttgtaacga
1021 atttttaaat tcacttttgt ttatttgtca gattgtaagt actttctcta atcacttttt
1081 tttcaaggca atcagggtat attatattgt acttcagcac agttttagag aacaattgtt
1141 ataattaaat gataaggtag aatatttctg catataaatt ctggctggcg tggaaatatt
1201 cttattggta gaaacaacta caccctggtc atcatcctgc ctttctcttt atggttacaa
1261 tgatatacac tgtttgagat gaggataaaa tactctgagt ccaaaccggg cccctctgct
1321 aaccatgttc atgccttctt ctctttccta cagctcctgg gcaacgtgct ggttgttgtg
1381 ctgtctcatc attttggcaa agaattccgc gggcggccgc tctagaggat ccgctagact
1441 gacatggtat tgctgcctgg gtccatgctt ctcacctcaa acctgcacca ccttcggcac
1501 cagatgagtc ctgggagctg gaaaagactg atcatcctct taagctgcgt attcggcggc
1561 ggcgggacga gtctgcaaaa taagaacccc caccagccca tgaccctcac ttggcaggta
1621 ctgtcccaaa ctggagacgt tgtctgggat acaaaggcag tccagccccc ttggacttgg
1681 tggcccacac ttaaacctga tgtatgtgcc ttggcggcta gtcttgagtc ctgggatatc
1741 ccgggaaccg atgtctcgtc ctctaaacga gtcagacctc cggactcaga ctatactgcc
1801 gcttataagc aaatcacctg gggagccata gggtgcagct accctcgggc taggactaga
1861 atggcaagct ctaccttcta cgtatgtccc cgggatggcc ggaccctttc agaagctaga
1921 aggtgcgggg ggctagaatc cctatactgt aaagaatggg attgtgagac cacggggacc
1981 ggttattggc tatctaaatc ctcaaaagac ctcataactg taaaatggga ccaaaatagc
2041 gaatggactc aaaaatttca acagtgtcac cagaccggct ggtgtaaccc ccttaaaata
2101 gatttcacag acaaaggaaa attatccaag gactggataa cgggaaaaac ctggggatta
2161 agattctatg tgtctggaca tccaggcgta cagttcacca ttcgcttaaa aatcaccaac
2221 atgccagctg tggcagtagg tcctgacctc gtccttgtgg aacaaggacc tcctagaacg
2281 tccctcgctc tcccacctcc tcttccccca agggaagcgc caccgccatc tctccccgac
2341 tctaactcca cagccctggc gactagtgca caaactccca cggtgagaaa aacaattgtt
2401 accctaaaca ctccgcctcc caccacaggc gacagacttt ttgatcttgt gcagggggcc
2461 ttcctaacct taaatgctac caacccaggg gccactgagt cttgctggct ttgtttggcc
2521 atgggccccc cttattatga agcaatagcc tcatcaggag aggtcgccta ctccaccgac
2581 cttgaccggt gccgctgggg gacccaagga aagctcaccc tcactgaggt ctcaggacac
2641 gggttgtgca taggaaaggt gccctttacc catcagcatc tctgcaatca gaccctatcc
2701 atcaattcct ccggagacca tcagtatctg ctcccctcca accatagctg gtgggcttgc
2761 agcactggcc tcaccccttg cctctccacc tcagttttta atcagactag agatttctgt
2821 atccaggtcc agctgattcc tcgcatctat tactatcctg aagaagtttt gttacaggcc
2881 tatgacaatt ctcaccccag gactaaaaga gaggctgtct cacttaccct agctgtttta
2941 ctggggttgg gaatcacggc gggaataggt actggttcaa ctgccttaat taaaggacct
3001 atagacctcc agcaaggcct gacaagcctc cagatcgcca tagatgctga cctccgggcc
3061 ctccaagact cagtcagcaa gttagaggac tcactgactt ccctgtccga ggtagtgctc
3121 caaaatagga gaggccttga cttgctgttt ctaaaagaag gtggcctctg tgcggcccta
3181 aaggaagagt gctgttttta catagaccac tcaggtgcag tacgggactc catgaaaaaa
3241 ctcaaagaaa aactggataa aagacagtta gagcgccaga aaagccaaaa ctggtatgaa
3301 ggatggttca ataactcccc ttggtttacc accttgatat ctaccattat gggacccctc
3361 attgtactcc tattgatttt gctcttcgga ccctgcattc ttaatcgatt agtccaattt
3421 gttaaagaca ggatatcagt ggtccaggct ctagttttga ctcaacaata tcaccagctg
3481 aagcctatag agtacgagcc atagctcgag ggcccagatc tttttccctc tgccaaaaat
3541 tatggggaca tcatgaagcc ccttgagcat ctgacttctg gctaataaag gaaatttatt
3601 ttcattgcaa tagtgtgttg gaattttttg tgtctctcac tcggaaggac atatgggagg
3661 gcaaatcatt taaaacatca gaatgagtat ttggtttaga gtttggcaac atatgcccat
3721 atgctggctg ccatgaacaa aggttggcta taaagaggtc atcagtatat gaaacagccc
3781 cctgctgtcc attccttatt ccatagaaaa gccttgactt gaggttagat tttttttata
3841 ttttgttttg tgttattttt ttctttaaca tccctaaaat tttccttaca tgttttacta
3901 gccagatttt tcctcctctc ctgactactc ccagtcatag ctgtccctct tctcttatgg
3961 agatccctcg acggatcggc cgcaattcgt aatcatgtca tagctgtttc ctgtgtgaaa
4021 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
4081 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
4141 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
4201 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
4261 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
4321 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
4381 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
4441 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
4501 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
4561 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
4621 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
4681 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
4741 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
4801 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
4861 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
4921 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
4981 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
5041 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
5101 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
5161 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
5221 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
5281 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
5341 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
5401 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
5461 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
5521 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
5581 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
5641 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
5701 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
5761 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
5821 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
5881 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
5941 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
6001 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
6061 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
6121 gcgcacattt ccccgaaaag tgccacctaa attgtaagcg ttaatatttt gttaaaattc
6181 gcgttaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc
6241 ccttataaat caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag
6301 agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc
6361 gatggcccac tacgtgaacc atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa
6421 gcactaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg
6481 aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt
6541 gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc
6601 gcgtcccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct
6661 tcgctattac gccagctggc gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg
6721 ccagggtttt cccagtcacg acgttgtaaa acgacggcca gtgagcgcgc gtaatacgac
6781 tcactatagg gcgaattgga gctccaccgc ggtggcggcc gctct
//
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