| 质粒名称: | pminiTol2 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 31829 |
| 存储实验室: | Stephen Ekker |
|
载体骨架基本信息 |
|
| 载体名称: | pDB739 |
| 载体抗性: | 氨苄霉素 |
| 空载体大小: | 3459 bp |
| 载体类型: | 克隆载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
文献引用方法 |
|
| 材料和方法部分: | pminiTol2 was a gift from Stephen Ekker (爱迪基因 plasmid # 31829) |
| 参考文献部分: | Harnessing a high cargo-capacity transposon for genetic applications in vertebrates. Balciunas D, Wangensteen KJ, Wilber A, Bell J, Geurts A, Sivasubbu S, Wang X, Hackett PB, Largaespada DA, McIvor RS, Ekker SC.. PLoS Genet. 2006 Nov 10;2(11):e169. Epub 2006 Aug 28. |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pminiTol2 3459 bp ds-DNA circular SYN 10-JAN-2018
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pminiTol2
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 3459)
AUTHORS Balciunas D, Wangensteen KJ, Wilber A, Bell J, Geurts A, Sivasubbu
S, Wang X, Hackett PB, Largaespada DA, McIvor RS, Ekker SC.
TITLE Harnessing a high cargo-capacity transposon for genetic applications
in vertebrates.
JOURNAL PLoS Genet. 2006 Nov 10;2(11):e169. Epub 2006 Aug 28.
COMMENT This file is created by Vector NTI
http://www.biofeng.com/
COMMENT ORIGDB|GenBank
COMMENT VNTAUTHORNAME|biofeng.com|
COMMENT VNTNAME|pminiTol2|
FEATURES Location/Qualifiers
source 1..3459
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter complement(584..602)
/label=SP6 promoter
/note="promoter for bacteriophage SP6 RNA polymerase"
primer_bind complement(585..602)
/label=SP6
/note="SP6 promoter, forward primer"
primer_bind complement(620..636)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(620..636)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(633..655)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 644..660
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(668..698)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 713..734
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(851..868)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(1022..1610)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(1102..1121)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(1781..2641)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 2404..2423
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(2642..2746)
/gene="bla"
/label=AmpR promoter
rep_origin complement(2824..3279)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(2961..2982)
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind 3173..3192
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 3405..3427
/label=M13/pUC Forward
/note="In lacZ gene"
primer_bind 3419..3436
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind 3420..3436
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 3443..3
/label=T7
/note="T7 promoter, forward primer"
promoter 3443..2
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
1 gggcgaattg ggcccagagg tgtaaagtac ttgagtaatt ttacttgatt actgtactta
61 agtattattt ttggggattt ttactttact tgagtacaat taaaaatcaa tacttttact
121 tttacttaat tacatttttt tagaaaaaaa agtacttttt actccttaca attttattta
181 cagtcaaaaa gtacttattt tttggagatc acttcattct attttccctt gctattacca
241 aaccaattga attgcgctga tgcccagttt aatttaaata gatctggcca tctagagcgg
301 ccgcgcgcac tagtgaattc catggatatc aagcttaaac aagaatctct agttttcttt
361 cttgctttta cttttacttc cttaatactc aagtacaatt ttaatggagt acttttttac
421 ttttactcaa gtaagattct agccagatac ttttactttt aattgagtaa aattttccct
481 aagtacttgt actttcactt gagtaaaatt tttgagtact ttttacacct ctgctcgacc
541 atatgggaga gctcccaacg cgttggatgc atagcttgag tattctatag tgtcacctaa
601 atagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
661 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga
721 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
781 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
841 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
901 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
961 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
1021 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
1081 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
1141 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
1201 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
1261 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
1321 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
1381 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
1441 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
1501 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
1561 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
1621 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
1681 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
1741 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
1801 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
1861 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
1921 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
1981 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
2041 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
2101 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
2161 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
2221 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
2281 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
2341 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
2401 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
2461 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
2521 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
2581 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
2641 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
2701 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
2761 aagtgccacc tgatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc
2821 aggaaattgt aagcgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct
2881 cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagaccg
2941 agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact
3001 ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac
3061 cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga
3121 gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga
3181 aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca
3241 ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc cattcgccat tcaggctgcg
3301 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
3361 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
3421 taaaacgacg gccagtgaat tgtaatacga ctcactata
//
