| 质粒名称: | punc-119c |
|---|---|
| 出品公司: | addgene |
| 目录编号: | 21595 |
| 存储实验室: | Al Fisher |
|
载体骨架基本信息 |
|
| 载体名称: | R6K origin PCR fragment |
| 载体出品公司: | Epicentre |
| 空载体大小: | 300bp |
| 完整质粒大小: | 3521 bp |
| 载体类型: | Worm Expression ; Adds unc-119 marker to Amp resistant plasmids |
| 筛选标记: | sunc-119 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | Pir116 |
| 拷贝数: | 低拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | unc-119 / kanamycin resistance |
| 物种来源: | C. elegans (nematode) |
| 插入大小: | 3200 bp |
| 基因突变: | N/A |
| GenBank编号 | NM_066998 |
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | BamHI(未破坏) |
| 3’克隆位点: | BamHi (未破坏) |
| 5’测序引物 | GCACTTTTCGGGGAAATGTGC |
| 3’测序引物 | GGTCTGACAGTTACCAATGCTTAATC |
|
文献引用方法 |
|
| 材料和方法部分: | punc-119c was a gift from Al Fisher (Addgene plasmid # 21595 ; http://n2t.net/addgene:21595 ; RRID:Addgene_21595) |
| 参考文献部分: | Retrofitting Ampicillin Resistant Vectors by Recombination for use in Generating C. elegans Transgenic Animals by Bombardment. Ferguson AA, Fisher AL. Plasmid. 2009 Jun 8. ():. 10.1016/j.plasmid.2009.06.001 PubMed 19520111 |
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载体基本信息
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载体简介
载体序列
LOCUS punc-119c 3521 bp ds-DNA circular SYN 13-MAY-2021
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS punc-119c
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 3521)
AUTHORS Ferguson AA, Fisher AL
TITLE Retrofitting Ampicillin Resistant Vectors by Recombination for use
in Generating C. elegans Transgenic Animals by Bombardment.
JOURNAL Plasmid. 2009 Jun 8. ():.
PUBMED 19520111
REFERENCE 2 (bases 1 to 3521)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..3521
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind 64..80
/label=pBluescriptKS
/note="For pBluescript vector"
primer_bind 65..81
/label=KS primer
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(2244..2260)
/label=SK primer
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(2244..2260)
/label=pBluescriptSK
/note="For pBluescript vector"
primer_bind complement(2352..2371)
/label=pENTR-R
/note="pENTR vectors, reverse primer"
CDS 2363..3178
/codon_start=1
/gene="aph(3')-Ia"
/product="aminoglycoside phosphotransferase"
/label=KanR
/note="confers resistance to kanamycin in bacteria or G418
(Geneticin(R)) in eukaryotes"
/translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
primer_bind complement(2436..2455)
/label=Kan-R
/note="Kanamycin resistance gene, reverse primer"
ORIGIN
1 ggatcccccg gggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
61 aactcgaggt cgacggtatc gataagcttc agtaaaagaa gtagaatttt atagtttttt
121 ttctgtttga aaaattctcc ccatcaatgt tctttcaaat aaatacatca ctaatgcaaa
181 gtattctata acctcatatc taaattcttc aaaatcttaa catatcttat cattgcttta
241 agtcaaacgt aacattaaaa aaaatgtttt ggaaaatgtg tcaagtctct caaaattcag
301 ttttttaaac cactcctata gtcctatagt cctatagtta cccatgaaat ccttatatat
361 tactgtaaaa tgtttcaaaa accattggca aattgccaga actgaaaatt tccggcaaat
421 tggggaaccg gcaaattgcc aatttgctga atttgccgga aacggtaatt gccgaaagtt
481 tttgacacga aaatggcaaa ttgtggtttt aaaatttttt tttttggaaa tttcagaatt
541 tcaattttaa tcggcaaaac tgtaggcatc ctaagaatgt tcctacatct attttgaaaa
601 gtaagcgaat tctatgaaaa tgtctaaaga aaatggggaa acaatttcaa aaaggcacag
661 tttcaatggt ttccgaatta tactaaatcc ctctaaaaac ttccggcaaa ttgatatccg
721 taaaagagca aatccgcatt tttgccgaaa attaaaattt ccgacaaatc ggcaaaccgg
781 caatttggcg aaatttgccg gaacgattgc cgcccacccc tgttccagag gttcaaactg
841 gtagcaaagc tcaaaatttc tcaaattctc caattttttt ttgaattttg gcagtgtacc
901 aaaatgacat tcagtcatat tggtttatta tagatttatt tagataaaat cctaaatgat
961 tctaccttta aagatgccca ctttaaaagt aatgactcaa acttcaaatt gctctaagat
1021 tctattgaat taccatcttt tcctctcatt ttctctcact gtctatttca tcacaaattc
1081 atccctctct cctctcttct ctctccctct ctctctcttt ctctttgctc atcatctgtc
1141 attttgtccg ttcctctctc tgcgccctca gcgttcccca cactctctcg cttctctttt
1201 cctagacgtc ttcttttttc atcttcttca gcctttttcg ccattttcca tctctgtcaa
1261 tcattacgga cgacccccat taatttttgg atgtgctctg cgagcaagag cacacggaac
1321 tcatttattt tttgttggaa tttctttttc attttagtta gttaaaagct acaacagcat
1381 atatgaaggc agagcaacaa caacaatcga tcgcacccgg ctcggcaacc ttcccgtctc
1441 agatgccccg acccccaccg gtaaccgaac aggctataac caccgaggcg gagcttctcg
1501 cgaaaaatca aattacacca aatgatgtgc tagctcttcc aggaatcact caaggattcc
1561 tatgctcccc atcggcaaac gtctataaca ttgagttcac caaattccaa atccgtgacc
1621 tcgacacgga acacgtgctc ttcgaaattg caaaaccgga aaatgagacg gaagagaatc
1681 tgcaggcgca agccgaatcg gcaagatatg tccgatatcg atttgcgccg aattttctga
1741 aattaaagac ggtcggcgcg acggtcgagt tcaaggtcgg cgatgtgccg atcacacatt
1801 ttcgaatgat tgaacggcac tttttcaagg atcgtttatt gaaatgcttt gattttgaat
1861 ttggattctg tatgccaaat tcgagaaaca actgtgaaca tatctatgaa tttccacaac
1921 tttcacaaca actcatggac gacatgatca ataatccaaa cgagacccgc tccgatagct
1981 tctattttgt ggagaataag ctcgtaatgc acaataaggc cgactactcg tatgatgcat
2041 aatttcccgc cgatggaata aaactaataa tttttctcgc aatttataga aatcatgtga
2101 ttttcctacc gctaaattta aaatttccta atttttcact atatttctca caaaatgcat
2161 tccgaaaact ctcatttttc agcttttcaa tacaaatccc aataaatgat ttttcaaata
2221 taaaaaaacg aattctgcag cccgatccac tagttctaga attgtgtctc aaaatctctg
2281 atgttacatt gcacaagata aaaatatatc atcatgaaca ataaaactgt ctgcttacat
2341 aaacagtaat acaaggggtg ttatgagcca tattcaacgg gaaacgtctt gctcgaggcc
2401 gcgattaaat tccaacatgg atgctgattt atatgggtat aaatgggctc gcgataatgt
2461 cgggcaatca ggtgcgacaa tctatcgatt gtatgggaag cccgatgcgc cagagttgtt
2521 tctgaaacat ggcaaaggta gcgttgccaa tgatgttaca gatgagatgg tcagactaaa
2581 ctggctgacg gaatttatgc ctcttccgac catcaagcat tttatccgta ctcctgatga
2641 tgcatggtta ctcaccactg cgatccccgg aaaaacagca ttccaggtat tagaagaata
2701 tcctgattca ggtgaaaata ttgttgatgc gctggcagtg ttcctgcgcc ggttgcattc
2761 gattcctgtt tgtaattgtc cttttaacag cgatcgcgta tttcgtctcg ctcaggcgca
2821 atcacgaatg aataacggtt tggttgatgc gagtgatttt gatgacgagc gtaatggctg
2881 gcctgttgaa caagtctgga aagaaatgca taaacttttg ccattctcac cggattcagt
2941 cgtcactcat ggtgatttct cacttgataa ccttattttt gacgagggga aattaatagg
3001 ttgtattgat gttggacgag tcggaatcgc agaccgatac caggatcttg ccatcctatg
3061 gaactgcctc ggtgagtttt ctccttcatt acagaaacgg ctttttcaaa aatatggtat
3121 tgataatcct gatatgaata aattgcagtt tcatttgatg ctcgatgagt ttttctaatc
3181 tagagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gacccccggg
3241 ggatccgaag atcagcagtt caacctgttg atagtacgta ctaagctctc atgtttcacg
3301 tactaagctc tcatgtttaa cgtactaagc tctcatgttt aacgaactaa accctcatgg
3361 ctaacgtact aagctctcat ggctaacgta ctaagctctc atggctaacg tactaagctc
3421 tcatgtttga acaataaaat taatataaat cagcaactta aatagcctct aaggttttaa
3481 gttttataag aaaaaaaaga atatataagg cttttaaagc t
//
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